Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (4): 669-678.doi: 10.3864/j.issn.0578-1752.2017.04.007

• PLANT PROTECTION • Previous Articles     Next Articles

Identification of Homeobox Transcription Factor Family in Genome-Wide and Expression Pattern Analysis of the Members in Setosphaeria turcica

ZHAO Jie1, ZHAO LiQing1, GONG XiaoDong1, FENG ShengZe1, LIU XingChen1, ZHENG YaNan1, LI ZhiYong2, SUN HaiYue1, WANG DongXue1, HAN JianMin1, GU ShouQin1, DONG JinGao1   

  1. 1Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, Hebei; 2Millet Research Institute, Hebei Academy of agriculture and Forestry, Shijiazhuang 050000
  • Received:2016-10-24 Online:2017-02-16 Published:2017-02-16

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Abstract: 【Objective】 The objectives of this study are to identify Homeobox transcription factor family from Setosphaeria turcica genome, and to predict the locations of their corresponding coding proteins in the cells, gene structure and evolutionary characteristics, as well as to explore their expression patterns at different growth and development stages in S. turcica. 【Method】Homeobox transcription factor family was identified based on S. turcica genome database and bioinformatics methods. Phylogenetic tree were created using the MEGA5.0 program. Gene structure characteristics were analyzed by GSDS software, which was a gene structure display server online (http://gsds1.cbi.pku.edu.cn/index.php). Amino acid sequence characteristics of the genes in Homeobox transcription factor family were compared using software Clustal X 1.83. The secondary structures of homeodomain proteins were predicted by employing software SOMPA. Three-dimensional structures of Homeobox transcription factor family were doped out using Software I-TASSER. Homeobox genes expression patterns were detected at different developmental stages based on quantitative real-time PCR (qRT-PCR) analysis. 【Result】 A total of 8 Homeobox genes were systematically identified from S. turcica genome and classified into 4 groups according to the gene structure and phylogenetic tree. Subcellular localization showed that all of the predicted proteins located in the cell nuclei. The family members contained conserved Homeobox domains and typical “helix-turn-helix” secondary structures. Different gene family members took on different expression levels at different development stages in S. turcica. The expression level of StHTF1 was significantly higher at mycelium, spore and appressorium formation stages than other stages. StHTF3, StHTF4 had the highest expression level at spore formation stage than other stages in S. turcica, while StHTF6 was the highest at germ tube formation stage. Moreover, StHTF2, StHTF5, StHTF7 and StHTF8 appeared higher expression level at appressorium formation stage than other genes. 【Conclusion】 Homeobox transcription factor family in S. turcica contains 8 genes which are classed into 4 categories and located in the nuclei. The proteins encoded by the genes contain conserved Homeobox domain and “helix-turn-helix” spatial structure. Homeobox gene family members in S. turcica appeared diverse expression profiles which will lay the foundation for identifying the functions of the gene family.

Key words: Setosphaeria turcica, Homeobox transcription factor family, system evolution, gene expression

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