外源玉米素对低温弱光胁迫下辣椒光合参数、抗氧化系统及玉米素合成相关基因表达的影响

doi:10.3864/j.issn.0578-1752.2025.19.012

Abstract

Abstract:

【Objective】The objective of this study is to explore the effects of exogenous zeatin (ZT) application at different concentrations on the tolerance and expression of ZT biosynthesis-related genes in pepper (Capsicum annuum) under low- temperature combined with low-light stress, and to reveal the mechanism of zeatin regulating the tolerance of pepper to low-temperature and low-light stress.【Method】The ‘Yangjiao No. 2’ pepper variety was used as the test material. After the seedlings had grown six true leaves, the leaves were sprayed four times with ZT solutions at concentrations of 0, 50, 100, 150, 200, and 250 μmol·L^-1. Then, the seedlings were transferred to a low-temperature combined with low-light environment simulated by an artificial climate chamber (temperature: 10 ℃/5 ℃ (day/night), light intensity: 100 μmol·m^-2·s^-1, photoperiod: 12 h/12 h (day/night)) for 7 d. Growth indices, chlorophyll content, photosynthetic parameters, chlorophyll fluorescence parameters, osmoregulatory substance levels (soluble sugars, soluble proteins, and proline), electrical conductivity (EC), reactive oxygen species (ROS) content, antioxidant enzyme activities (POD, SOD, and CAT), expression of ZT biosynthesis-related genes and endogenous ZT content were measured.【Result】Exogenous ZT application significantly enhanced plant height, stem diameter, root length, and both aboveground and underground fresh weights under stress conditions. Additionally, ZT treatment significantly increased chlorophyll content, photosynthetic parameters and chlorophyll fluorescence parameters. It also elevated the levels of osmoregulatory substances and the activities of key antioxidant enzymes, while reducing ROS accumulation. Furthermore, exogenous ZT induced the upregulation of ZT biosynthesis-related genes, including CaCKX6, CaCYP375A1, CaZOX, and CaZOG, and increased the content of endogenous ZT in pepper, which improved the low-temperature combined with low-light tolerance. Among the tested concentrations, 150 μmol·L^-1 ZT was identified as the most effective concentration.【Conclusion】Exogenous application of 150 μmol·L^-1 ZT effectively enhanced osmoregulatory substance content, stabilized chlorophyll fluorescence parameters, increased antioxidant enzyme activity, induced the upregulation of ZT biosynthesis-related genes and the production of endogenous ZT, thereby improving the resistance of pepper to low-temperature combined with low-light and alleviating the adverse impacts of low-temperature combined with low-light stress in pepper.

Key words: exogenous zeatin, low-temperature combined with low-light, pepper (Capsicum annuum), photosynthetic parameter, antioxidant system, gene expression

ZHANG Jie, HU ChenXi, QI JianBo, ZHANG YongTai, CHEN YiBo, ZHANG YongJi. Effects of Exogenous Zeatin on Photosynthetic Parameter, Antioxidant System and Expression of Genes Related to Zeatin Synthesis in Pepper Under Low-Temperature Combined with Low-Light Stress[J].Scientia Agricultura Sinica, 2025, 58(19): 3959-3969.

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References 36

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引物名称Primer name	引物序列Primer sequence (5′→3′)		用途Usage
RT-CaCKX6	F: TCCATCCGAAATCCGTTGC	R: CTTGCGCCTGGCCTAGAAG	表达量分析Expression level analysis
RT-CaCYP375A1	F: ATGGCAGGATAAAGTTCGT	R: CACTGTGATGTATGGCAAG	表达量分析Expression level analysis
RT-CaZOX	F: CTACTAAGAAGAGGCAACC	R: TATCAAGCCATCTCAAGCA	表达量分析Expression level analysis
RT-CaZOG	F: TGGCTCATTCGTCCACAGG	R: CAAGCAGCTATTGGCACCC	表达量分析Expression level analysis
Actin	F: TGTCCATCTGCTCTCTGTTG	R: CACCCCAAGCACAATAAGAC	内参基因Reference gene

指标Index	CK	T1	T2	T3	T4	T5	T6
株高Plant height (cm)	9.33±0.17a	8.12±0.13d	8.95±0.14b	9.26±0.09ab	9.32±0.18a	8.87±0.09b	8.36±0.05bc
茎粗Stem diameter (mm)	2.30±0.08a	2.07±0.04e	2.18±0.02c	2.27±0.02b	2.31±0.09a	2.20±0.02c	2.13±0.02de
根长Root length (cm)	19.85±1.12a	10.29±0.78e	15.29±0.84d	18.30±0.70b	19.87±1.19a	18.17±1.05b	15.62±1.49c
地上部分鲜重 Aboveground fresh weight (g)	5.19±0.28a	4.14±0.06g	4.40±0.14e	4.72±0.12c	4.99±0.15ab	4.67±0.08cd	4.22±0.08f
地下部分鲜重 Underground fresh weight (g)	2.42±0.05a	1.87±0.09e	2.25±0.08c	2.36±0.08b	2.43±0.08a	2.37±0.04b	2.11±0.07d

Effects of Exogenous Zeatin on Photosynthetic Parameter, Antioxidant System and Expression of Genes Related to Zeatin Synthesis in Pepper Under Low-Temperature Combined with Low-Light Stress

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