Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (1): 161-169.doi: 10.3864/j.issn.0578-1752.2014.01.017

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

GDF-10 Gene Polymorphism and Its Association with Production Traits in Gannan Yak

 LI  Tian-Ke-1, 2 , LIANG  Chun-Nian-1, 2 , LANG  Xia-1, 2 , PEI  Jie-1, 2 , WU  Xiao-Yun-1, 2 , CHU  Min-1, 2 , QIN  Wen-1, 2 , YAN  Ping-1, 2   

  1. 1.Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences，Lanzhou 730050;
    2.Key Laboratory of Yak Breeding Engineering，Lanzhou 730050)
  • Received:2013-05-22 Online:2014-01-01 Published:2013-06-20

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Abstract: 【Objective】Growth differentiation factor-10 (GDF-10) is a member of the TGF-beta super family and the bone morphogenetic protein family (BMP). It is originally identified by PCR using degenerate oligonucleotides based on the BMP-3 sequence. Studies suggested that the GDF-10 gene plays a key role in skeletal morphogenesis. The objective of this study was to identify the polymorphisms of GDF-10 gene and their correlation with production traits was investigated for the purpose of the breeding of Gannan yak. 【Method】 Thirty samples were randomly selected from genomic DNA of 298 Gannan yak to construct DNA pool. Nine primers were designed to amplify the exons 1, 2 and 3 of GDF-10 gene. Effective PCR production was purified by DNA gel extraction kit and direct sequencing subsequently. BLAST and Chromas were used to screen the SNPs in GDF-10 gene by the wave height of sequencing map. The genotypes were determined by high-resolution melting curre (HRM) and the frequency of alleles was estimated. The association between genotypes and body measurement was evaluated by software SPSS13.0. Three SNPs (T132C, G672T and G1204A) of GDF-10 gene were identified in the population, which were at extrons 1,extron 2, and extron 3, respectively. The PHASE and SHEsis softwares were used to analyze matching chain disequilibrium and haplotype analysis, respectively. The SPSS17.0 software was used for association analysis. 【Result】Three SNPs, 12116 (G/A), 12152 (C/T) and 13041 (T/C) were discovered in exon 3. Through population genetics analysis, the results showed that three locals were at low polymorphic status (PIC＜0.25). The ?2 tests showed that the locus of 13041 (T/C) was not all in the status of Hardy-Weinberg equilibrium (P＜0.05), the other two loci were all in the status of Hardy-Weinberg equilibrium (P＞0.05). Analysis of matching chain disequilibrium and haplotype analysis show that there is a weak chain of balance between the three locals. Analysis of association of polymorphism with body measurement trait at all mutation loci show that there were significant effects on body height, body length, weight and herrt girth (P＜0.05 or P＜0.001) and there is no effects on tube girth (P＞0.05). Seven haplotype combinations were found in 298 Gannan yak, the ATC and ATT have significant effects on body measurement traits and weight. 【Conclusion】 The results suggest that three SNPs and two haplotype combinations might have potential effects on production traits in the above mentioned yak populations and could be used for marker-assisted selection.

Key words: yak , GDF-10 gene , polymorphism , HRM , haplotypes , body measurement traits

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