牦牛早期胚胎核旁斑点形成及对后续发育的影响

doi:10.3864/j.issn.0578-1752.2023.06.014

Abstract

Abstract:

【Objective】 The aim of present study was to identify of paraspeckle formation stages during the early embryonic development in yak (Bos grunniens). Furthermore, the long non-coding RNAs (LncRNAs) involved in paraspeckle formation were determined, and the effects and regulatory mechanism of their formation on the subsequent developmental ability of yak embryos were studied. 【Method】 The yak embryos were produced by in vitro fertilization (IVF), DAPI staining of embryonic nuclei combined with paraspeckle protein 1 (PSPC1) mRNA detection were done by quantitative real-time fluorescence PCR (qRT-PCR) at different stages in order to confirm paraspeckle formation. PSPC1 protein in embryos was verified by immunofluorescence technique. The levels of encoding nuclear paraspeckle assembly transcript 1 (NEAT1), coactivator associated arginine methyl transferase 1 (CARM1) and non-POU domain containing octamer-binding protein (p54nrb) mRNAs were also detected by qRT-PCR at different stages. The mRNA level of PSPC1 in zygote was inhibited by RNA interference technology, and the developmental rate of embryos in subsequent stages was compared. The blastocyst quality was evaluated by analyzing the number of total cells, trophoblast cells (TE) and inner cell mass (ICM). B-cell lymphoma/leukemia-2 (Bcl-2) and b-cell lymphoma/leukemia associated X protein (Bax) in blastocysts form in the control and PSPC1 mRNA interference groups was detected. 【Result】 (1) Paraspeckle could be observed in the nuclei of embryos at all different stages; however, nuclei could be more clearly seen at 2-cell stage and 4-cell stage. The PSPC1 mRNA was higher in yak embryos from 2-cell to morula stage, which was the highest in embryos at 4-cell embryos and morula. The fluorescence intensity of PSPC1 protein was the strongest in embryos from those stages. (2) The levels of NEAT1, CARM1 and p54nrb mRNA were higher from 2-cell to morula stage than that from other stages. NEAT1 and p54nrb were found to be highest in embryos at 4-cell stage, while CARM1 was not significantly different from 2-cell to morula stage (P>0.05). (3) The developmental rates of morula and blastocyst in PSPC1 mRNA interference group were reduced, which was more significantly reduced in morula rate. The total number of blastocyst cells in PSPC1 mRNA interference group was significantly lower than that in the control group, which was mainly caused by ICM reduction. There was no significant difference in number of TE between the two groups. (4) The levels of Bax mRNA and protein were enhanced in blastocyst form PSPC1 mRNA interference group, while the levels of Bcl-2 mRNA and protein were reduced in blastocyst, and the cell lysis was observed in ICM. 【Conclusion】 The paraspeckle was formed at 2-cell to morula stage transition in the yak embryo, which was more prominent in 4-cell stage. The expression of PSPC1, NEAT1, CRAM1 and p54nrb in the stages of paraspeckle formation were on high levels. Interference with PSPC1 mRNA in yak zygotes resulted in decreased developmental ability of subsequent embryo. The blastocyst quality was also reduced by inducing apoptosis of inner cell mass, which was also involved in the regulation of cell fate determination in early embryo development.

Key words: yak, paraspeckle, cell fate, apoptosis, LncRNAs

PAN YangYang, WANG JingLei, WANG Meng, WANG LiBin, ZHANG Qian, CHEN Rui, ZHANG TianTian, CUI Yan, XU GengQuan, FAN JiangFeng, YU SiJiu. Formation and Function of Paraspeckle During Pre-implantation Embryos Development in Yak[J].Scientia Agricultura Sinica, 2023, 56(6): 1189-1203.

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References 37

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基因 Gene	正义链序列 Sense sequence	反义链序列 Antisense sequence
siRNA1	AAAUGCUUGCUCUAGUAGCUC	GCUACUAGAGCAAGCAUUUUC
siRNA2	AGGUUUUGCUGCAAAUUCCAC	GGAAUUUGCAGCAAAACCUCC
siRNA3	UUCUCUUUCCUUAUGAUACUG	GUAUCAUAAGGAAAGAGAACA

基因 Gene	引物序列 Primer sequence（5'→3'）	T_m (℃)	产物大小 Amplicon size (bp)	GenBank登录号 GenBank accession No.
NEAT1	F:GAACTTGTCAATACCAGCAGC	56	291	XR_001351403.1
NEAT1	R: CAACAACTTTCCCGTCTTACC	56	291	XR_001351403.1
PSPC1	F :GGACATCACCGAGGACGACT	60	145	NM_001075277.1
PSPC1	R: GCCATCCAGCTCTGCTTTTG	60	145	NM_001075277.1
P54nrb	F:CTTTATCCGCTTGGAAACAC	55	209	NM_001046554.1
P54nrb	R:CCACAATGACTACAGCCCTC	55	209	NM_001046554.1
CARM1	F:GTCATTCATCATCACCCTG	50	274	XM_024994894.1
CARM1	R:AATCTTGTCCTTGAAGTCTGT	50	274	XM_024994894.1
Bax	F:TTTGCTTCAGGGTTTCATC	59	174	NM173894.1
Bax	R:CAGCTGCGATCATCCTCT	59	174	NM173894.1
Bcl-2	F: CTGCACCTGACGCCCTTCAC	62	236	NM001166486.1
Bcl-2	R: GCGTCCCAGCCTCCGTTGT	62	236	NM001166486.1
β-actin	F:CTTCAACACCCCTGCCAT	60	238	JF830811
β-actin	R: CTCGGCTGTGGTGGTGAAG	60	238	JF830811

组别 Group	4细胞胚胎数 No. of 4-cell embryos	桑葚胚（发育率） Morula (Development rate, %)	囊胚率（发育率） Blastocyst (Development rate, %)
Control	113	85（75.22 ± 3.32a）	35（41.17 ± 2.23%a）
siRNA1	115	67（58.26 ± 1.86b）	22（32.83 ± 1.54%b）

组别 Group	总细胞数量 Total no. of cells	TE细胞数量 No. of TE cells	ICM细胞数量 No. of ICM cells
Control	102.45 ± 3.08a	81.40 ± 5.02a	21.07 ± 0.58a
siRNA1	98.80 ± 2.65b	80.00 ± 1.56a	18.80 ± 1.45b

Formation and Function of Paraspeckle During Pre-implantation Embryos Development in Yak

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