Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (1): 161-169.doi: 10.3864/j.issn.0578-1752.2014.01.017

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

GDF-10 Gene Polymorphism and Its Association with Production Traits in Gannan Yak

 LI  Tian-Ke-1, 2 , LIANG  Chun-Nian-1, 2 , LANG  Xia-1, 2 , PEI  Jie-1, 2 , WU  Xiao-Yun-1, 2 , CHU  Min-1, 2 , QIN  Wen-1, 2 , YAN  Ping-1, 2   

  1. 1.Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences，Lanzhou 730050;
    2.Key Laboratory of Yak Breeding Engineering，Lanzhou 730050)
  • Received:2013-05-22 Online:2014-01-01 Published:2013-06-20

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Abstract: 【Objective】Growth differentiation factor-10 (GDF-10) is a member of the TGF-beta super family and the bone morphogenetic protein family (BMP). It is originally identified by PCR using degenerate oligonucleotides based on the BMP-3 sequence. Studies suggested that the GDF-10 gene plays a key role in skeletal morphogenesis. The objective of this study was to identify the polymorphisms of GDF-10 gene and their correlation with production traits was investigated for the purpose of the breeding of Gannan yak. 【Method】 Thirty samples were randomly selected from genomic DNA of 298 Gannan yak to construct DNA pool. Nine primers were designed to amplify the exons 1, 2 and 3 of GDF-10 gene. Effective PCR production was purified by DNA gel extraction kit and direct sequencing subsequently. BLAST and Chromas were used to screen the SNPs in GDF-10 gene by the wave height of sequencing map. The genotypes were determined by high-resolution melting curre (HRM) and the frequency of alleles was estimated. The association between genotypes and body measurement was evaluated by software SPSS13.0. Three SNPs (T132C, G672T and G1204A) of GDF-10 gene were identified in the population, which were at extrons 1,extron 2, and extron 3, respectively. The PHASE and SHEsis softwares were used to analyze matching chain disequilibrium and haplotype analysis, respectively. The SPSS17.0 software was used for association analysis. 【Result】Three SNPs, 12116 (G/A), 12152 (C/T) and 13041 (T/C) were discovered in exon 3. Through population genetics analysis, the results showed that three locals were at low polymorphic status (PIC＜0.25). The ?2 tests showed that the locus of 13041 (T/C) was not all in the status of Hardy-Weinberg equilibrium (P＜0.05), the other two loci were all in the status of Hardy-Weinberg equilibrium (P＞0.05). Analysis of matching chain disequilibrium and haplotype analysis show that there is a weak chain of balance between the three locals. Analysis of association of polymorphism with body measurement trait at all mutation loci show that there were significant effects on body height, body length, weight and herrt girth (P＜0.05 or P＜0.001) and there is no effects on tube girth (P＞0.05). Seven haplotype combinations were found in 298 Gannan yak, the ATC and ATT have significant effects on body measurement traits and weight. 【Conclusion】 The results suggest that three SNPs and two haplotype combinations might have potential effects on production traits in the above mentioned yak populations and could be used for marker-assisted selection.

Key words: yak , GDF-10 gene , polymorphism , HRM , haplotypes , body measurement traits

[1]张容昶. 中国的牦牛. 甘肃: 甘肃科学技术出版社, 1989.

Zhang R C. China: The Yak. Gansu: Gansu Science and Technology Publishing House, 1989. (in Chinese)

[2]陆仲璘. 牦牛育种及高原肉牛业. 甘肃名族出版社, 1994.

Lu Z L. Yak Breeding and Beef Raising on Tibetan Pleteau. Gansu Nationalities Publishing House, 1994. (in Chinese)

[3]Hino J, Takao M, Takeshita N, Konno Y, Nishizawa T. cDNA cloning and genomic structure of human bone morphogenetic protein-3B (BMP-3b). Biochemical and Biophysical Research Communications, 1996, 223(2): 304-310.

[4]Cunningham N S, Jenkins N A, Gilbert D J. Copeland N G. Growth differentiation factor-10:a new member of the transforming growth factor-beta superfamily related to bone morphogenetic protein-3. Growth Factors, 1995, 12(2): 99-109.

[5]Zhao R, Lawler A M, Lee S J. Characterization of GDF-10 expression patterns and null mice. Developmental Biology, 1999, 212: 68-79.

[6]Hino J, Matsuo H, Kangawa K. Bone morphogenetic protein-3b (BMP-3b)gene expression is correlated with differentiation in rat calvarial osteoblasts. Biochemical and Biophysical Research Communications, 1999, 256(2): 419-424.

[7]Hino J, Nishimatsu S, Nagai T, Matsuo H. Coordination of BMP-3b and cerberus isrequired for head formation of Xenopus embryos. Developmental Biology, 2003, 260(1): 138-157.

[8]Eugene G, Hales B F. Retinoic acid receptor gamma-induced misregulation of chondrogenesis in the murine limb bud in vitro. Toxicological Sciences, 2008, 106(1): 223-232.

[9]Adoligbe C, Zan L, Farougou S. Bovine GDF10 gene polymorphism analysis and its association with body measurement traits in Chinese indigenous cattle. Molecular Biology Reports, 2012, 39(4): 4067-4075.

[10]Rothschild M F, Soller M. Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe Newsletter for Agricultural Genomics, 1997, 8: 13-20.

[11]Shin S C, Chung E R. Association of SNP marker in the leptin gene with carcass and meat quality traits in Korean cattle. Asian-Australas Animal Science, 2007, 20: 1-6.

[12]Emma J G, Stuart E D, Jagadish P. Functional gene analysis suggests different acetogen populations in the bovine rumen and tammar walla by forestomach. Applied and Environmental Microbiology, 2010, 76(23): 7785-7795.

[13]Li M, Chen Q, Sun G, Shi X, Zhao Q, Zhang C, Zhou J, Qin N. Characterization and expression of bone morphogenetic protein 4 gene in postnatal pigs. Molecular Biology Reports, 2010, 37(5): 2369-2377.

[14]Chu M, Jia L, Zhang Y, Jin M. Polymorphisms of coding region of BMPR-IB gene and their relationship with litter size in sheep. Molecular Biology Reports, 2011, 38(6): 4071-4076.

[15]Chu M X, Lu L, Feng T, Di R. Polymorphism of bone mor -phogenetic protein 4 gene and its relationship with litter size of Jining Grey goats. Molecular Biology Reports, 2010, 37(8): 3921-3929.

[16]Chu M X, Zhao X H, Zhang Y J. Polymorphisms of BMPR-IB gene and their relationship with litter size in goats. Molecular Biology Report , 2010, 37(8): 4033-4039.

[17]李爱民, 马云, 杨东英, 蓝贤勇.ANGPTL6基因的3个多态位点与其生长性状的关联性分析.中国农业科学, 2012, 45(11): 2306-2314.

Li A M, Ma Y, Yang D Y, Lan X Y. 3 Polymorphisms of BMPR-IB gene and its relationship with body measurement traits. Scientia Agricultura Sinica, 2012, 45(11): 2306-2314. (in Chinese)

[18]Michael L, Robert P, Robert P. Genotyping of single-nucleotide polymorphisms by high- resolution melting of small amplicons. Molecular Diagnosticsand Genetics, 2004, 50(7): 1156-1164.

[19]Ye M H, Chen J L, Zhao G P. Associations of A-FABP and H-FABP markers with the content of intramuscu -lar fat in Beijing-you chicken, Animal Biotechnology, 2010, 21: 14-24.

[20]Cameron N, Steven F. Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons. Nucleic Acids Research, 2008, 1093(10): 1-8.

[21]Nei M, Roychoudhury A K. Sampling variances of heterozygosity and genetic distance. Genetics, 1974, 76(2): 379-390.

[22]Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America, 1979, 76(10): 5269-5273.

[23]Botstein D, White R L, Skolnick M, Davis R. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 1980, 32(3): 314-331.

[24]Takao M, Hino J, Takeshita N, Konno Y. Identification of rat bone morphogenetic protein-3b (BMP-3b), a new member of BMP-3. Biochemical and Biophysical Research Communications, 1996, 219: 656-662.

[25]Kingsley D M. The TGF-p superfamily: new members,new receptors, and new genetic tests of function in different organisms. Genes and Development, 1994 8: 133-146.

[26]Wozney J M, Rosen V, Celeste A J, Mitsock L M. Novel regulators of bone formation: molecular clones and activities. Science, 1988,242: 1528-1534.

[27]Liu Y F, Zan L S, Li K, Zhao S P. A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds. Molecular Biology Reports, 2010, 37: 429-434 .

[28]Feng T, Geng C X, Lang X Z. Polymorphisms of caprine GDF9 gene and their association with litter size in Jining Grey goats. Molecular Biology Reports, 2011, 38: 5189-5197.
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