Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 3921-3931.doi: 10.3864/j.issn.0578-1752.2012.19.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Validation of QTL for Oil Content in a Population of Worldwide Rapeseed Cultivars by Association Analysis

 SUN  Zhong-Yong, CHENG  Shuang, WANG  Ji-Bian, HUANG  Ji-Xiang, CHEN  Fei, NI  Xi-Yuan, ZHAO  Jian-Yi   

  1. 1.浙江省农业科学院作物与核技术利用研究所，杭州 310021
    2.杭州师范大学生命与环境科学学院，杭州 310036
    3.浙江大学农业与生物技术学院，杭州 310058
  • Received:2012-05-02 Online:2012-10-01 Published:2012-07-25

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Abstract: 【Objective】 The objective of this study is to validate the genetic stability of 7 major QTL for oil content，which were detected in authors’ previous researches, and to assess the feasibility of marker assisted selection for improving seed oil content using closely linked functional makers.【Method】Fourteen lipid related candidate gene markers located in the 7 QTL regions were chosen to amplify 81 core collections of Brassica napus and then a linkage disequilibrium was estimated by marker-trait association analysis. Meanwhile, the allelic distribution among 81 world-wide cultivars in each QTL was investigated.【Result】By association analysis, significant differences in oil content were detected between favorable and unfavorable allele genotypes in each of six linked markers flanking four QTL (OilA1, OilA5, OilA7 and OilC8-1), respectively. Over 5 percentage in oil content could be increased by assembling favorable alleles together through four QTL linked markers Ra2E04，ZAAS919，ZAAS828 and ZAAS441. According to the marker genotypes, in the loci of OilA1 and OilC8-1, higher than 80% of European cultivars have carried positive alleles from “Sollux”, while 80% and 60% Chinese materials contain unfavorable alleles. On OilA5 and OilA7, almost all the tested European varieties did not show favorable alleles from “Gaoyou” and for Chinese materials, also only around 20%-30% cultivars carrying these positive alleles.【Conclusion】Six orthologous gene markers (corresponding to At3g51830, At2g42450, At2g44620,At1g73600, At1g73480 and At1g13560), which closely link to OilA1, OilA5, OilA7 and OilC8-1 might be functional important in the accumulation of oil content in rapeseed. When favorable alleles from three QTL including OilA7 or all four selected QTL combined together, the oil content could be increased more than 5 percentages. The results showed that the positive alleles in screened out 4 QTL loci are potentially interesting for present breeding programs in China, while the Chinese alleles from OilA5 and OilA7 should be also attractive to rapeseed breeders in European countries.

Key words: Brassica napus, oil content, association analysis, marker assistant selection (MAS)

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