Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (20): 4279-4283.doi: 10.3864/j.issn.0578-1752.2011.20.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

The Expression of FcRn mRNA in Mammary Gland Epithelial Cells of Holstein Cows Treated with Different Concentrations of Thyroxine, Insulin, and Glucagon in Vitro

 HU  Xiao-Li, ZHANG  Chun-Lin, WANG  Jia-Qi, ZHAO  Sheng-Guo, ZHANG  Yu-Tao, BU  Deng-Pan, ZHOU  Ling-Yun   

  1. 1.中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室
  • Received:2010-08-30 Online:2011-10-15 Published:2010-11-26

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Abstract: 【Objective】The mRNA expression of FcRn was studied in Holstein Cows mammary gland epithelial cells treated with hormone by real-time PCR. 【Method】 FcRn mRNA expression of in vitro cultured mammary epithelial cells treated with different concentrations of thyroxine hormone (0.01, 0.1, 1 μmol•L-1), insulin (0.005, 0.1, 0.5 μmol•L-1) and glucagon (0.01, 0.1, 1 μmol•L-1), respectively, was determined.【Result】The results showed that the mRNA abundance of FcRn increased with the higher dose of thyroxine and glucagon significantly (P＜0.05) in a certain range. However, the mRNA expression of FcRn was significantly lower when treated with 0.005 μmol•L-1 and 0.5 μmol•L-1 insulin than 0.1μmol•L-1 (P＜0.05).【Conclusion】Thyroxine, insulin and glucagon can obviously change the level of FcRn mRNA, which provide a basis for further research of the change and influence of FcRn.

Key words: HolsteinCows, mammaryepithelialcells, hormone, real-timePCR, mRNA

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