Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (20): 4284-4294.doi: 10.3864/j.issn.0578-1752.2011.20.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Pyramiding Effect and Preliminary Verification of Candidate Genes for Intramuscular Fat Traits in Chickens

 CHANG  Guo-Bin, LIU  Xiang-Ping, CHEN  Rong, LUAN  De-Qin, WANG  Ke-Hua, ZHANG  Ying, MA  Teng, ZHOU  Wei, DAI  Ai-Qin, CHEN  Guo-Hong   

  1. 1.扬州大学动物科学与技术学院
    2.中国农业科学院家禽研究所
  • Received:2011-04-14 Online:2011-10-15 Published:2011-07-14

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Abstract: 【Objective】This paper was designed to analyze the pyramiding effect and preliminary verification of A-FABP、Ex-FABP, which are the key candidate genes for intramuscular fat (IMF) traits in chickens, and to provide the basis of exploration and utilization of effective molecular markers on chicken fatty trait. 【Method】Single nucleotide polymorphisms (SNPs) in all exons of Ex-FABP and A-FABP were detected with PCR-SSCP technology, and association between genotype and IMF content in breast muscle was conducted. Developmental expression pattern of singular and pyramiding genotypes in segregating progeny population were studied with fluorescence quantitative real-time PCR, and then preliminary verification of genetic effects of pyramiding genotypes were conducted.【Result】The results showed that, there is A/G mutation in exon 3 of A-FABP and exon 4 of Ex-FABP gene, respectively, and three genotypes were found totally. AA (A-FABP) and BB (Ex-FABP) were the dominant genotypes associated with high IMF content in breast muscle, and AABB was the dominant pyramiding genotype. The results of Q-PCR from segregating progeny population confirmed that there was no significant difference between male and female in expression pattern of two genes. In breast muscle, expression of two genes reached top point at 4 weeks old, decreased rapidly at 8 weeks old and rise slowly at 12 weeks old. Expression level of AABB genotype was the highest in all the pyramiding genotype individuals, and ABAB genotype was lowest, which was consistent with the results of singular genes’ expression and the difference in IMF contents. Thus, it proved that there was pyramiding effects in the two genes, and the AABB genotype had close association with the high IMF content in breast muscle.【Conclusion】A-FABP and Ex-FABP gene and their pyramiding genotype could be used as available candidate markers on IMF content trait in chicken.

Key words: chicken, intramuscularfat, genotype, pyramidingeffect, verification

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