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Table of Content

    15 October 2011, Volume 44 Issue 20
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Status and Trends of Rice Science Based on Bibliometrics
    WU Ya-Wen, XIA Xiao-Dong, ZHI Gui-Ye, GE Lei, HE Jian-Mei, E Zhi-Guo, LI Jian, WANG Lei
    Scientia Agricultura Sinica. 2011, 44(20):  4129-4141.  doi:10.3864/j.issn.0578-1752.2011.20.001
    Abstract ( 1042 )   PDF (722KB) ( 2247 )   Save
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    【Objective】 Analyses of status and trends of rice science in the world based on bibliometrics were performed for providing information to rice researchers and decision makers. 【Method】 The published rice articles from China, the United States, the United Kingdom, Japan and India as well as other countries during 1998 and 2009 based on the Scopus database were analyzed. 【Result】 A total of 42 307 rice articles were identified from more than 100 countries, meanwhile, the number of cited times for each article and the H indices for each country, affiliations and authors were retrieved. 【Conclusion】 The output of rice articles in the world and the rates of rice articles to the total number of world articles increased year by year, especially in China. In the five countries under study, the cited rate and the H indices of the rice articles, as well as the high cited article rates from the United States and the United Kingdom were the highest, those from Japan were higher than the average values of the world, and those from China and India were below the average values of the world. There were more core authors in Japan, China and the United States. The effects of the rice articles from the International Rice Research Institute, Zhejiang University in China, National Institute of Agrobiological Sciences in Japan and Cornell University in USA were stronger. The research hotspot in rice was molecular biology.
    Research on Heat and Drought Tolerance in Rice (Oryza sativa L.) by Overexpressing Transcription Factor OsbZIP60
    YU Xu, NIU Xiang-Li, YANG Sheng-Hui, LI Yu-Xiang, LIU Liang-Liang, TANG Wei, LIU Yong-Sheng
    Scientia Agricultura Sinica. 2011, 44(20):  4142-4149.  doi:10.3864/j.issn.0578-1752.2011.20.002
    Abstract ( 974 )   PDF (889KB) ( 1586 )   Save
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    【Objective】Dissect biological function of transcription factor OsbZIP60 by transgenic technology, making preparation for developing stress tolerance rice to reduce the hazard caused by heat and drought stresses.【Method】The overexpression vector containing the full-length coding region of OsbZIP60 gene was constructed and introduced into rice cv. Nipponbare by Agrobacterium-mediated genetic transformation. The biological function of OsbZIP60 was analyzed by observing the phenotypes of transgenic and wild type plants under stress conditions. The expression pattern of OsbZIP60 and its expression level in transgenic line was analyzed by semi-quantitative RT-PCR and real-time quantitative PCR.【Result】The expression level of OsbZIP60 in overexpression transgenic lines is distinctly increased compared to that of WT plants. OsbZIP60 can be induced by heat, and it’s further up-regulated in transgenic lines under stress condition. OsbZIP60 is constitutively expressed in rice and has the highest abundance in leaves. Transgenic plants showed significant enhanced stress tolerance. Detached aerial parts of transgenic plants also showed a slower water loss rate compared to that of WT plants.【Conclusion】Transcription factor OsbZIP60 plays an important role in response to heat and drought. Overexpression of OsbZIP60 might be utilized in genetic improvement of heat and drought tolerance in rice.
    Cloning and Expression Analysis of A Glutathione-S-transferase Gene in The Latex of Hevea brasiliensis (para rubber tree)
    FAN Yu-Jie, LIN Fei-Peng, AN Ze-Wei, TANG Chao-Rong
    Scientia Agricultura Sinica. 2011, 44(20):  4150-4158.  doi:10.3864/j.issn.0578-1752.2011.20.003
    Abstract ( 719 )   PDF (591KB) ( 1080 )   Save
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    【Objcetive】The objective of this study is to clone the cDNA and genomic clone of a glutathione-S-transferase (GST) from rubber tree, and characterize its structural and phylogenetic characteristics, investigate its expression patterns under various abiotic stresses and hormone treatments. 【Method】Combined with the latex EST database, RT-PCR and PCR were used to clone the full length cDNA and genomic DNA of a Hevea GST, respectively. On-line bioinformatics tools were used to predict the conserved functional domains and subcellular localization. Clustal X was used to construct the phylogenetic tree. Real-time RT-PCR (qRT-PCR) was used to characterize the expression patterns of the Hevea GST gene under various treatments (tapping, wounding, chilling, hormone and tapping panel dryness). 【Result】The full-length cDNA (793 bp) encoding a 25.4 kD (219 aa) Tau GST protein was firstly cloned from the latex of H. brasiliensis, named HbGSTU1 and deposited in GenBank (JF729318). The genomic DNA (1 313 bp) of HbGSTU1 was also cloned, which contains one intron (520 bp) and two exons. HbGSTU1 protein contained the Tau GST-specific N-terminal domain (G site) and the C-terminal domain (H site). HbGSTU1 was predicted to be a cytoplasmic protein, which shared the highest amino acid identity (85%) with a GST from Ricinus communis. The qRT-PCR results show that HbGSTU1 expression was regulated by tapping, wounding, 4℃ chilling, 2,4-D and Ethrel, but not by the three hormones JA, SA and ABA. 【Conclusion】HbGSTU1 is a typical Tau GST protein, and is implicated in stress-responses in Hevea tree, which may serve as a target gene for Hevea molecular breeding.
    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY
    Studies on the Characteristics of Dry Matter Production and Accumulation of Rice Varieties with Different Productivity Levels
    MA Qun, YANG Xiong, LI Min, LI Guo-Ye, ZHANG Hong-Cheng, DAI Qi-Gen, HUO Zhong-Yang, XU Ke, WEI Hai-Yan, GAO Hui
    Scientia Agricultura Sinica. 2011, 44(20):  4159-4169.  doi:10.3864/j.issn.0578-1752.2011.20.004
    Abstract ( 863 )   PDF (406KB) ( 953 )   Save
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    【Objective】 The characteristics of dry matter and photosynthetic production of different rice varieties under their best-fit nitrogen levels were studied. 【Method】 The field experiment was carried out with 50 early-maturing late Japonica rice varieties, adopted in the region of Yangtze River, under seven nitrogen levels (0, 150.0, 187.5, 225.0, 262.5, 300.0, 337.5 kg•hm-2) to investigate the difference of dry matter accumulation. The highest value of dry matter accumulation under different nitrogen levels was defined as the most productivity of N fertilizer group (MPNG). Then according to MPNG, rice varieties could be classified into four types including low type, middle type, high type and top type. On this basis, the dry matter accumulation, LAI, photosynthetic potential, crop growth rate and net assimilation rate of the four types rice varieties were studied. 【Result】 The main results were as follows: In the range of 0-337.5 kg•hm-2 nitrogen application, the dry matter accumulation at jointing and heading of 50 rice varieties were significantly increased, while the dry matter accumulation at maturity increased firstly and reduced afterward with the  nitrogen application increasing. Population dry matter accumulation at jointing stage decreased as the productivity level increasing, for the dry matter accumulation of top and high levels were increasing much greater than middle and low levels after jointing, there was a little difference among the 4 productivity levels at heading stage. The dry matter accumulation of top productivity level was significantly higher than the other three productivity level varieties at maturity. There was a significant negative correlation between dry matter accumulation and MPNG at early growth stages, while there was a significant positive correlation between them at the middle and later stages of rice growth. The dry matter accumulation at sowing-jointing stage decreased as the productivity level increasing, while it was increased at jointing-heading and heading-maturity stage. The ratio at each stage changed accordantly with the dry matter accumulation. The economic coefficient of different productivity level varieties significantly increased as the productivity level increasing. Before jointing, the differences of leaf area index and photosynthetic potential of 4 productivity level varieties were tiny, while the population growth rate and the net assimilation rate had a significant degressive tendency; at Jointing-heading and heading-maturity stage, leaf area index, photosynthetic potential, the population growth rate and the net assimilation rate were all increased as productivity level increasing. 【Conclusion】 The dry matter production of top level varieties at heading-maturity stage have obvious advantages, they have high leaf area index, photosynthetic potential, population growth rate and net assimilation rate. 
    Effect of Daily Minimum Temperatures on Sucrose Metabolism of Cotton Fiber During Secondary Wall Thickening in Xinjiang
    TIAN Jing-Shan, BAI Yu-Lin, HU Xiao-Bing, LUO Hong-Hai, ZHANG Ya-Li, ZHAO Rui-Hai, ZHANG Wang-Feng
    Scientia Agricultura Sinica. 2011, 44(20):  4170-4179.  doi:10.3864/j.issn.0578-1752.2011.20.005
    Abstract ( 769 )   PDF (497KB) ( 527 )   Save
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    【Objective】 It is of great significance to explore the technical approach to reveal underlying mechanisms contributing to cotton fiber strength changes through analyzing the impact of minimum temperature of averages daily on sucrose metabolism, cellulose deposition, and strength formation in Xinjiang.【Method】The impact of different temperatures on the involved substances content in sucrose metabolism during cotton fiber development, and the changes of key enzymes in sucrose metabolism and their correlation with cellulose deposition, were studied through setting different sowing dates. 【Result】 Under normal sowing date, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) in the boll fiber in the middle of plant were high. As a result, sucrose converted completely and cellulose accumulated sustainably and steadily. Thus, cellulose content had high and strength increase. Under postponing sowing date, the boll development suffered low temperature at night. The activity of sucrose phosphate synthase decreased, and the activities of acid invertase (AI) and alkaline invertase (NI) increased. Consequently, fructose increased significantly and cellulose deposition was influenced. 【Conclusion】 During the cotton fiber thickening period, <15.3℃ of average daily minimum temperature influenced the sucrose metabolism enzyme activities, consequently, conversion efficiency of sucrose decreased. Thus, the circulation rate of fructose was lower and fructose was enriched in fibers.
    Trend Analysis of Typical Phenophases of Major Crops Under Climate Change in the Three Provinces of Northeast China
    LI Zheng-Guo, YANG Peng, TANG Hua-Jun, WU Wen-Bin, CHEN Zhong-Xin, ZHOU Qing-Bo, ZOU Jin-Qiu, ZHANG Li
    Scientia Agricultura Sinica. 2011, 44(20):  4180-4189.  doi:10.3864/j.issn.0578-1752.2011.20.006
    Abstract ( 827 )   PDF (606KB) ( 1078 )   Save
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    【Objective】 The objective of this research is to analyze the phenophases (including the stages of seeding, heading and maturity) changes of rice, maize, spring wheat and soybean in three provinces of Northeast China. 【Method】Annual slope change rate (θ) was computed to evaluate the temporal trend and the relationship between agroclimate and phenophases. 【Result】Over the past 20 years, the timing of first effective temperature date (T≥10℃) was advanced, the first frost date was postponed, the growth period was continually extended and the accumulated temperature (T≥10℃) was increased in most area of the three provinces. Under this background, the temporal trend of phenophases of both rice and maize showed an advanced seeding stage (0.04<θ<0.55 d?a-1 and 0.04<θ<0.35 d?a-1), a postponed maturity stage (0.09<θ<0.35 d?a-1 and 0.23<θ<0.38 d?a-1) and an extended length of growth period (0.31<θ<1.26 d?a-1 and 0.11<θ<0.57 d?a-1). By contrast, temporal trend of soybean phenophases was different and characterized by an advanced seeding stage (0.01<θ<0.61 d?a-1), a maturity stage (0.18<θ<0.19 d?a-1), and a shortened growth period (0.06<θ<0.17 d?a-1). As for spring wheat, there was no obvious temporal change for the phenophases.【Conclusion】Climate change resulted in the increase of temperature suitability for crops, which could benefit from an early seeding stage, a late maturity stage and a long growth period.
    PLANT PROTECTION
    Genetic Diversity of Phytophthora sojae in China Based on RFLP
    XIAO Jiang-Tao, MIAO Miao, GAO Kun, XIANG Gui-Lin, YANG Shuai, DONG Sha-Meng, WANG Yuan-Chao, WANG Ke-Rong
    Scientia Agricultura Sinica. 2011, 44(20):  4190-4198.  doi:10.3864/j.issn.0578-1752.2011.20.007
    Abstract ( 922 )   PDF (580KB) ( 748 )   Save
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    【Objective】 The objective of this study is to explore the genetic diversity among Phytophthora sojae strains from soybean-growing regions in China and America. 【Method】 Restriction fragment length polymorphism (RFLP) was used to examine the genetic relationships among strains of P. sojae collected from 5 different regions. Nei’s genetic distances among the strains of P. sojae were calculated, and cluster analysis using UPGMA method was carried out by using the software package NTSYSpc V2.10. The Popgene V1.32 was used for calculation of genetic identity. 【Result】 RFLP probe PS127558 was selected and 25 fragments were detected, of which 24 was polymorphic, leading to the identification of 2 multilocus RFLP groups amongst the strains from Xinjiang and Inner Mongolia. RFLP group A which also exists in Heilongjiang occurred in over 88.2% of all samples collected from Xinjiang and 100% from Inner Mongolia, respectively. Genetic identity among populations of Xinjiang and Heilongjiang or Inner Mongolia and Heilongjiang is very high. Shannon’s information index revealed that the Heilongjiang populations have a higher genetic diversity than that of the Xinjiang and Inner Mongolia ones. RFLP probe PS127558 detected 17 fragments and 12 of which were polymorphic, among the 22 Fujian P. sojae strains, which lead to the distinction of 2 multilocus RFLP groups. Genetic identity is very low among the populations of Fujian and any of the other regions. The P. sojae strains between Heilongjiang and America show a high level of genetic identity, and several strains with shared DNA fingerprints were also found between Heilongjiang and America.【Conclusion】 P. sojae in Xinjiang and Inner Mongolia might have been introduced from Heilongjiang, and that in Fujian might belong to a separate evolutionary branch or originated from other area. In addition, exchange of P. sojae strains also occured between Heilongjiang and America.
    Development of Duplex PCR Assay for Detection of Clavibacter michiganensis subsp. sepedonicus and  Pectobacterium atroseptica from Potato
    HAN Guang-Tao, YANG Zhi-Hui, ZHU Jie-Hua, ZHAO Dong-Mei, HAN Yan-Qing
    Scientia Agricultura Sinica. 2011, 44(20):  4199-4206.  doi:10.3864/j.issn.0578-1752.2011.20.008
    Abstract ( 727 )   PDF (574KB) ( 863 )   Save
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    【Objective】The objective of this study is to develop a duplex PCR system to simultaneously and reliably detect Clavibacter michiganensis subsp. sepedonicus and Pectobacterium atroseptica. 【Method】 Choosing the cellulose A gene sequence encoded by the native plasmid pCS1 of C. michiganensis subsp. sepedonicus which was published on the GenBank and comparing it with the nucleotide sequence of closely-related species and some pathogens of potato, a specific pair of primers, CMS1/CMS2, was designed and synthesized. A duplex PCR system had been established under the optimized PCR parameters using the combining primers CMS1/CMS2 and ECA1f/ECA2r which was a specific pair of PCR primers to detect P. atroseptica. 【Result】 Using CMS1/CMS2 primers, a single unique PCR band of 913 bp was amplified from C. michiganensis subsp. sepedonicus. The detection sensitivity was 100 fg•μL-1 of DNA and 105 CFU•mL-1 of bacteria. Under the duplex PCR system, the 913 and 690 bp PCR bands from P. atroseptica could be specifically amplified. The detection sensitivity was 600 fg•μL-1 of DNA and 5×105 CFU•mL-1 of bacteria.【Conclusion】The duplex PCR system could simultaneously and rapidly detect the two pathogens.
    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Characteristics of Nutrient Input/Output and Nutrient Balance in Different Regions of China
    LI Shu-Tian, JIN Ji-Yun
    Scientia Agricultura Sinica. 2011, 44(20):  4207-4229.  doi:10.3864/j.issn.0578-1752.2011.20.009
    Abstract ( 1573 )   PDF (667KB) ( 3033 )   Save
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    Clearance of nutrient input/output and balance in farmland in different regions of China is critical for nutrient management, scientific distribution and application of fertilizer resources, and improving nutrient use efficiency, etc. Based on existed literatures this paper systematically analyzed and discussed the current nutrient resources and application status, estimated the amount of manures, crop straws and their potential amount of nutrients NPK. The characteristics of nutrient input, output and balance in different regions of China were analyzed and evaluated.  Strategies and suggestions on nutrient management were discussed based on the existed issues of nutrient input and output.
    Effects of Long-term Fertilization on Diversity of Ammonia-Oxidizing Archaea Communities and Abundance in Dry Highland Soil of Loess Plateau
    WU Chuan-Dong, XIN Liang, LI Xiu-Ying, WANG Bao-Li, QU Dong
    Scientia Agricultura Sinica. 2011, 44(20):  4230-4239.  doi:10.3864/j.issn.0578-1752.2011.20.010
    Abstract ( 849 )   PDF (541KB) ( 862 )   Save
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    【Objective】In order to improve the efficiency of nitrogen utilization and explicate the function of ammonia- oxidizing archaea (AOA) under the changes of soil quality in the Loess Plateau, the community structure diversity and abundance of AOA were studied. 【Method】 The influence of long-term fertilization treatments including CK, M, NM, PM and NPM on soil AOA community structure diversity and amoA gene copy numbers were analyzed by restriction fragment length polymorphism (PCR-RFLP) and real-time PCR.【Result】From the clone libraries of the different fertilization treatments, there were 25, 18, 29, 20 and 30 restriction endonuclease types, respectively. The α diversity indices indicated that there was a pronounced difference among five fertilizer treatments. The OTUs was the highest in NPM treatment and the lowest diversity in M treatment. The rescaled distance matrix tree indicated that the different fertilization had the largest convergence coefficient of AOA community types with the CK treatment soil, so the different fertilization led to significant changes of AOA communities. The amoA gene copy numbers of AOA changes were different among the treatments, whereas the highest copy numbers were detected in the NPM treatment, and had a pronounced difference with other fertilizer treatments. All preponderant sequences of AOA fell within soils/fresh water sediments based on phylogenetic tree of amoA gene amino acid sequences analysis.【Conclusion】Long-term fertilization resulted in changes of AOA community diversity and abundance.
    Calibration of SCS-CN Initial Abstraction Ratio of a Typical Small Watershed in the Loess Hilly-Gully Region
    ZHOU Shu-Mei, LEI Ting-Wu
    Scientia Agricultura Sinica. 2011, 44(20):  4240-4247.  doi:10.3864/j.issn.0578-1752.2011.20.011
    Abstract ( 945 )   PDF (468KB) ( 713 )   Save
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    【Objective】 The initial abstraction ratio, as one of the basic inputs of SCS-CN method, plays an important role in watershed rainfall-runoff simulation. In this study, attempt was made to determine the initial abstraction ratio of a typical small watershed in the Loess hilly-gully region, in order to supply some references for regional applicability assessment of SCS-CN method. 【Method】In SCS-CN method, the proportion of initial abstraction (Ia) to the maximum retention (S) is defined as initial abstraction ratio, which was believed to have a standard value of 0.2. However, many studies indicate that variations of the ratio exist in different regions. In this study, 14 typical rainstorms in 1987-2006 in Qiaozi-West watershed were selected to calculate the initial abstraction ratio using Back Calculation (BC) and Event Analysis (EA) methods.【Result】Results showed that most of the values calculated by the two methods were lower than the standard value of 0.2. Moreover, the value (0.17) determined by EA method was a little higher than that (0.1) by BC method. By assigning 0.1, 0.17 and 0.2 to initial abstraction ratio, the runoff depth of each study event was calculated, respectively. Through five evaluation criteria of Pearson correlation coefficient (r), model efficiency coefficient (E), relative error (RE), absolute error (AE) and curve fitting, 0.1 was found to be the appropriate value for the watershed initial abstraction ratio. The results indicated that the derived initial abstraction ratio by the two methods differed markedly from the standard value. This may be due to the discrepancies in geologic, geomorphologic and climatic circumstances between the loess hilly-gully region and the originally intended areas of SCS-CN method. 【Conclusion】 It is essential to calibrate the initial abstraction ratio using locally observed data prior to SCS-CN method application.
    HORTICULTURE
    Photosynthetic Response to the Root-Knot Nematode Meloidogyne incognita in Resistant Cultivar Sour Cucumber (Cucumis hystrix Chakr.)
    YE De-You, QIAN Chun-Tao, CHEN Jin-Feng
    Scientia Agricultura Sinica. 2011, 44(20):  4248-4257.  doi:10.3864/j.issn.0578-1752.2011.20.012
    Abstract ( 838 )   PDF (548KB) ( 646 )   Save
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    【Objective】Sour cucumber shows a high resistance to the root-knot nematode Meloidogyne incognita. To make clear on the photosynthetic responsive characteristics of its resistance will provide a theoretical basis for isolation and functional identification of resistance gene. 【Method】 The effects of nematode infection on photosynthesis, chlorophyll fluorescence parameters and physiological indices related of cucumber leaves resistant and susceptible to M. incognita were investigated by using seedling inoculation in greenhouse.【Result】 Nematode infection induced a significantly lower decrease of cucumber leaf chlorophyll (Chl) content in resistant Sour cucumber than in susceptible Beijingjietou. Decreased extents of Chl content with nitrogen content reduction in Sour cucumber leaves were less than in Beijingjietou. Net photosynthetic rate(Pn), stomatal conductance (Gs) decreased in two varieties leaves after nematode infection, intercellular CO2 concentration (Ci) of leaves decreased in Sour cucumber, while its value have a sharp rise in Beijingjietou. Gs reduction in response to relative water content (RWC) declining in Sour cucumber leaves was more sensitive than Beijingjietou. Nematode infection led to a lower reduction of actual photochemical efficiency (ΦPSⅡ) and photochemical quenching (qP), while induced a significantly higher increase of non-photochemical quenching (qN) in Sour cucumber than in Beijingjietou. Nematode infection has little effect on the initial slope of Pn/Ci in Sour cucumber, leaving a sharp decrease in Beijingjietou. 【Conclusion】 The results indicated that there is a lower decrease of chlorophyll content in Sour cucumber, so as to maintain a higher net photosynthetic rate in resistant compared with susceptible ones. All these did not cause significant effect on the plant growth of Sour cucumber.
    Establishment of Walnut Paternity Test
    ZHOU Bei-Bei, MA Qing-Guo, WANG Hua, WANG Wei, ZHANG Mei-Yong, PEI Dong
    Scientia Agricultura Sinica. 2011, 44(20):  4258-4264.  doi:10.3864/j.issn.0578-1752.2011.20.013
    Abstract ( 888 )   PDF (312KB) ( 961 )   Save
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    【Objective】A simple, rapid and efficient walnut paternity method was established to resolve the problems of unknown paternal and confused parents in breeding ,which provided reference for other varieties. 【Method】'Luguo-2' was used as material. Phenotypic traits and SSR markers were conducted to analyse parentage relationship between 'Luguo-2' and its alleged parents.【Result】The phenotypes with statistics of eight nut stable expressional characters failed to get parent-child correlation results. In genotype results, the heredity of 'Shangsong-6', 'Luxiang' and 'Luguo-2' accords with Mendelian genetic regularity, there may be a paternity. PI and RCP wre analyzed by using the results of 10 SSR primer pairs, and the cumulative RCP was 99.997%. In addition, the appraisal result between ‘Slz-40’ and its known parent was consistent with the reality. 【Conclusion】'Shangsong-6' and 'Luxiang' were the parents of 'Luguo-2' with SSR clustering results supporting the conclusion. The parents of 'Luguo-2' were identified by SSR genotypes and thus providing an effective method to search and identify the parents of other trees and fruit varieties.
    Establishment of Synthetic Assessing System for the Quality of Spray Cut Chrysanthemum
    HAN Yong, YE Yan-Ping, CHEN Fa-Di, FANG Wei-Min, CHEN Su-Mei, YAO Jian-Jun, GUAN Zhi-Yong, WANG Chun-Xin
    Scientia Agricultura Sinica. 2011, 44(20):  4265-4271.  doi:10.3864/j.issn.0578-1752.2011.20.014
    Abstract ( 975 )   PDF (305KB) ( 696 )   Save
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    【Objective】The present study was to establish a synthetic system for assessing the quality of spray cut chrysanthemum, and to offer technological support for selecting, introducing, and cross breeding of spray cut chrysanthemum.【Method】Field experiments were conducted to observe and count quality factors of 160 varieties of spray cut chrysanthemum in 2009 and 2010. A synthetic assessing system was established by means of expert consultation, analytic hiberarchy process and K-Means cluster method.【Result】The factors affecting the quality of spray cut chrysanthemum were analyzed, and 11 factors were selected. The weight of the 11 factors was quantitatively evaluated by analytic hierarchy process. Plant character, flower color and branch length had the most important effect, and weight was 0.228, 0.150, and 0.132, respectively. One hundred and sixty varieties of spray cut chrysanthemum were then clustered into 4 grades by K-Means cluster method. There were 41 varieties graded excellent, 71 graded good, 35 graded moderate and 13 graded poor,the occupancy was 25.63%, 44.38%, 21.88% and 8.13%, respectively.【Conclusion】The evaluation result was consistent with production practice, and the system constructed by adopting expert consultation, analytic hiberarchy process and cluster method can be used to evaluate and classify for spray cut chrysanthemum varieties.
    STORAGE·FRESH-KEEPING·PROCESSING
    Analysis on Characteristics of Near Infrared Spectra of Beef According to Regions and Feeding Periods
    CAI Xian-Feng, GUO Bo-Li, WEI Yi-Min, SUN Shu-Min, ZHAO Duo-Yong, WEI Shuai
    Scientia Agricultura Sinica. 2011, 44(20):  4272-4278.  doi:10.3864/j.issn.0578-1752.2011.20.015
    Abstract ( 920 )   PDF (402KB) ( 708 )   Save
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    【Objective】The feasibility of using near infrared spectroscopy(NIRS) to differentiate beef from different regions and feeding periods was discussed, and the objective was to provide analytical methods and theoretical support for geographical origin assignment and authentication of beef. 【Method】 Eighteen cattle muscle samples obtained from designed migration and feeding model experiment in Taipusiqi (Inner Mongolia Autonomous Region), Yangling Zone(Shaanxi province) and Nanyang City (Henan province) were scanned by DA7200 NIR spectrometer, and the differences of NIR spectra were analyzed. Principle component analysis was used to analyze the spatial distribution of samples. 【Result】 NIR spectra of beef samples were closely related to factors such as region, feed and feeding practice, and there were significant differences in NIR spectra of samples from different regions and feeding periods. The results indicated that samples could be differentiated according to their regions and feeding periods. 【Conclusion】NIR spectra of beef are significantly affected by regions and feeding periods, which have to be taken into account for geographical origin assignment and authentication. It is feasible to differentiate beef from different regions and feeding periods using NIRS analysis.
    ANIMAL SCIENCE·RESOURCE INSECT
    The Expression of FcRn mRNA in Mammary Gland Epithelial Cells of Holstein Cows Treated with Different Concentrations of Thyroxine, Insulin, and Glucagon in Vitro
    HU Xiao-Li, ZHANG Chun-Lin, WANG Jia-Qi, ZHAO Sheng-Guo, ZHANG Yu-Tao, BU Deng-Pan, ZHOU Ling-Yun
    Scientia Agricultura Sinica. 2011, 44(20):  4279-4283.  doi:10.3864/j.issn.0578-1752.2011.20.016
    Abstract ( 736 )   PDF (265KB) ( 521 )   Save
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    【Objective】The mRNA expression of FcRn was studied in Holstein Cows mammary gland epithelial cells treated with hormone by real-time PCR. 【Method】 FcRn mRNA expression of in vitro cultured mammary epithelial cells treated with different concentrations of thyroxine hormone (0.01, 0.1, 1 μmol•L-1), insulin (0.005, 0.1, 0.5 μmol•L-1) and glucagon (0.01, 0.1, 1 μmol•L-1), respectively, was determined.【Result】The results showed that the mRNA abundance of FcRn increased with the higher dose of thyroxine and glucagon significantly (P<0.05) in a certain range. However, the mRNA expression of FcRn was significantly lower when treated with 0.005 μmol•L-1 and 0.5 μmol•L-1 insulin than 0.1μmol•L-1 (P<0.05).【Conclusion】Thyroxine, insulin and glucagon can obviously change the level of FcRn mRNA, which provide a basis for further research of the change and influence of FcRn.
    Pyramiding Effect and Preliminary Verification of Candidate Genes for Intramuscular Fat Traits in Chickens
    CHANG Guo-Bin, LIU Xiang-Ping, CHEN Rong, LUAN De-Qin, WANG Ke-Hua, ZHANG Ying, MA Teng, ZHOU Wei, DAI Ai-Qin, CHEN Guo-Hong
    Scientia Agricultura Sinica. 2011, 44(20):  4284-4294.  doi:10.3864/j.issn.0578-1752.2011.20.017
    Abstract ( 673 )   PDF (555KB) ( 673 )   Save
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    【Objective】This paper was designed to analyze the pyramiding effect and preliminary verification of A-FABP、Ex-FABP, which are the key candidate genes for intramuscular fat (IMF) traits in chickens, and to provide the basis of exploration and utilization of effective molecular markers on chicken fatty trait. 【Method】Single nucleotide polymorphisms (SNPs) in all exons of Ex-FABP and A-FABP were detected with PCR-SSCP technology, and association between genotype and IMF content in breast muscle was conducted. Developmental expression pattern of singular and pyramiding genotypes in segregating progeny population were studied with fluorescence quantitative real-time PCR, and then preliminary verification of genetic effects of pyramiding genotypes were conducted.【Result】The results showed that, there is A/G mutation in exon 3 of A-FABP and exon 4 of Ex-FABP gene, respectively, and three genotypes were found totally. AA (A-FABP) and BB (Ex-FABP) were the dominant genotypes associated with high IMF content in breast muscle, and AABB was the dominant pyramiding genotype. The results of Q-PCR from segregating progeny population confirmed that there was no significant difference between male and female in expression pattern of two genes. In breast muscle, expression of two genes reached top point at 4 weeks old, decreased rapidly at 8 weeks old and rise slowly at 12 weeks old. Expression level of AABB genotype was the highest in all the pyramiding genotype individuals, and ABAB genotype was lowest, which was consistent with the results of singular genes’ expression and the difference in IMF contents. Thus, it proved that there was pyramiding effects in the two genes, and the AABB genotype had close association with the high IMF content in breast muscle.【Conclusion】A-FABP and Ex-FABP gene and their pyramiding genotype could be used as available candidate markers on IMF content trait in chicken.
    Promoting Effect of IGF-I on Prehierarchical Follicle Development in Laying Chickens
    JIA Yu-Dong, YAN Fei-Fei, ZENG Wei-Dong, ZHANG Cai-Qiao
    Scientia Agricultura Sinica. 2011, 44(20):  4295-4301.  doi:10.3864/j.issn.0578-1752.2011.20.018
    Abstract ( 722 )   PDF (465KB) ( 815 )   Save
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    【Objective】The aim of the experiment is to investigate the effect of IGF-I on prehierarchical follicle development from laying chickens.【Method】IGF-I receptor (IGF-IR) expression in granulosa and theca cells was studied in the prehierarchical follicles of laying chickens via immunohistochemistry and RT-PCR. In addition, the effects of IGF-I on small yellow follicle (SYF) development were investigated. 【Result】The expression of IGF-IR gradually increased in both granulosa and theca layers from the prehierarchical follicles. Moreover, IGF-IR displayed the highest expression in SYF. Compared with control, treatment with IGF-I and FSH alone or in combination significantly increased cell density, thickness of granulosa and theca layers, stimulated the expression IGF-IR in both granulosa and theca layer from SYF. Furthermore, IGF-I and FSH displayed an additive effect. 【Conclusion】 These results indicate that IGF-I may increase follicular cell number, thickness of granulosa and theca layers and stimulated IGF-IR mRNA expression in SYF. In addition, IGF-I exhibited synergistic effect with FSH to enhance prehierarchical follicular cell proliferation. Therefore, these results may facilitate delineating the mechanism of intra-ovarian endocrine regulation of poultry follicle development.
    VETERINARY SCIENCE
    Generation and Identification of the Monoclonal Antibody Against Fumonisin B1
    WANG Ying, ZHENG Hao, HE Cheng-Hua, ZHANG Ai-Hua, FENG Lu-Lu, HUANG Chao-Ying, ZHANG Hai-Bin
    Scientia Agricultura Sinica. 2011, 44(20):  4302-4308.  doi:10.3864/j.issn.0578-1752.2011.20.019
    Abstract ( 769 )   PDF (438KB) ( 645 )   Save
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    【Objective】 Mycotoxins can lead to serious food security issues. This study focused on the generation of the specific monoclonal antibody (McAb) against fumonisin B1. 【Method】 The artificial antigen of fumonisin B1 was prepared and a hybridoma cell line was screened with method of hybridoma. Monoclonal antibody was generated by murine ascites and identified. 【Result】 A hybridoma cell line of F3 was screened. The antibody secreted by F3 was classified into IgG1 and the light chain type was κ; the results of 10% SDS-PAGE showed that the molecular weight of high chain was 50 kD and the light chain was 25 kD, respectively. The titer of McAb was 1:3 200 in supernatant of F3 and 1:51 200 in ascites with indirect enzyme-linked immunosorbent assay. The affinity was 1.60×10-8 mol•L-1 and the value of IC50 was 3.589 ng•mL-1. There were no cross reactions to other mycotoxins. The high specificity of antibody was identified by western blotting. 【Conclusion】The McAb against FB1 in this experiment are of sensitivity and specificity.
    Effects of Different Immune Status on Blood Biochemical Parameters and Cellular Immune Response in Broiler Chickens
    FENG Yan, YANG Xiao-Jun, WANG Xiao-Fei, QIN Ding-Kui, YIN Rui-Qing, YAO Jun-Hu
    Scientia Agricultura Sinica. 2011, 44(20):  4309-4316.  doi:10.3864/j.issn.0578-1752.2011.20.020
    Abstract ( 789 )   PDF (426KB) ( 844 )   Save
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    【Objective】 This experiment was conducted to determine the effects of different immune statuses on blood biochemical parameters and cellular immune response of broiler chickens. 【Method】 One hundred and eighty 1-day-old AA broilers were allocated into four groups. Each treatment had 5 replicate cages of 9 broilers per replicate pen. This study will set different immune stress models, including the control group (no vaccines, NV), conventional vaccines group (CV), immune hyperthyroidism group (LPS) and immune suppression group (CYP). The T lymphocytes and subgroups were isolated from periphery blood from immunized and unvaccinated birds and were detected by CD3/CD4/CD8 triple labelling by flow cytometric analysis and MTT assays, respectively.【Result】The results showed that different immunological statuses had no significant effect on feed conversion ratio of broiler chickens among 4 treatment (P>0.05). LPS group and CYP group had significantly lower broiler body weight gain and feed intake than NV group (P<0.05). Serum TP, GLU, and CHLO contents were no significantly different among treatments (P>0.05). On d 21 and 35, ALB of LPS and CYP groups was significantly lower than CV and NV groups (P<0.05), and ALB levels of NV and CV groups were not significantly different (P>0.05). On d 21, α-AGP of LPS group was significantly lower than CV group (P<0.05), BUN of LPS group was significantly higher than NV group (P<0.05). CPY and LPS inhibited the T, B lymphocyte proliferation, and CD4+/CD8+ T cell percentage of LPS group was significantly lower than NV group (P<0.05). 【Conclusion】 Chickens with no vaccines maintained normal growth performance and immune response. LPS stress increased the cellular immune response of chickens. Tolerance of chicken to CYP increased with age.
    Study on Polymorphisms of IRAK2 Gene and Its Association with Mastitis in Chinese Holstein Cattle
    SUN Shu-Xia, WANG Chang-Fa, ZHANG Lian-Jiang, JU Zhi-Hua, HUANG Jin-Ming, LI Jian-Bin, ZHONG Ji-Feng, ZHANG Jia-Bao, LI Qiu-Ling
    Scientia Agricultura Sinica. 2011, 44(20):  4317-4325.  doi:10.3864/j.issn.0578-1752.2011.20.021
    Abstract ( 685 )   PDF (678KB) ( 787 )   Save
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    【Objective】 The associations among five single nucleotide polymorphisms (SNPs) in interleukin-1 receptor associated kinase 2 (IRAK-2) gene and somatic cell score (SCS) in Chinese Holstein Cattle were investigated. 【Method】 A herd of 1 292 dairy cows, 129 Luxi Yellow Cattle and 32 Bohai Black Cattle from Shandong province and Tianjin were used as experimental materials. Polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP), created restriction site (CRS)-PCR and sequencing methods were applied to analyze the polymorphisms of five loci (g.28879, g.28916, g.29212, g.40035, and g.400120) in IRAK-2 gene. The associations between SNPs and SCS were analyzed by the least-squares method as applied in the GLM procedure of SAS 8.1 and haplotype analysis was performed by SHEsis software.【Result】The results of haplotype analysis of five SNPs showed that 30 different haplotypes and 71 haplotype combinations were identified. The value of SCS of individuals with H21H23 (TTAGGCTCCC) was the lowest through the result of association between polymorphisms of IRAK-2 gene and SCS. Statistical analyses revealed significant associations between genotype CC and CT in position 28 879, and between GG and AG in position 28 916. The SCS value of genotype CC and GG was lower than that of CT and AG, respectively (P<0.05), whereas no significant differences were detected among the positions 29212, 40035, 40120 and SCS (P>0.05).【Conclusion】The haplotype combination H21H23 was a favorable haplotype combination to the SCS. It may be used as a possible candidate for the molecular markers of mastitis resistance in dairy cattle breeding program.
    RESEARCH NOTES
    Effects of Elevated CO2 and Enhanced UV-B on Epidemic Components of Wheat Stripe Rust
    HAN Guo-Fei, WANG Hai-Guang, MA Zhan-Hong
    Scientia Agricultura Sinica. 2011, 44(20):  4326-4332.  doi:10.3864/j.issn.0578-1752.2011.20.022
    Abstract ( 641 )   PDF (366KB) ( 503 )   Save
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    【Objective】The objective of this research is to investigate the effects of elevated CO2 concentration and enhanced UV-B radiation on epidemic components of wheat stripe rust in climate chamber and to provide a basis for the prediction and control of the disease in future climate conditions. 【Method】 Different wheat cultivars were used as materials including Mingxian 169, Jing 9428, Jing 0045 and Jingdong 8 in this experiment. The wheat seedlings were inoculated with CYR32, one of the physiological races of the pathogen. Four treatments including elevated CO2 and enhanced UV-B radiation were conducted to find out the effects of CO2 and UV-B radiation on epidemic components of wheat stripe rust.【Result】Under high CO2 concentration conditions, the infection efficiency of wheat stripe rust on Mingxian 169, Jing 9428 and Jing 0045 was significantly increased, latent period was shortened, and lesion expansion was increased. Enhanced UV-B radiation had no significant effect on the disease epidemic components. Under the interaction of CO2 concentration and UV-B radiation, the changes of epidemic components of wheat stripe rust on the four cultivars were not significant compared with the control.【Conclusion】Elevated CO2 concentration could increase the occurrence of wheat stripe rust. However, enhanced UV-B radiation could not affect the disease significantly. The comprehensive action of elevated CO2 concentration and enhanced UV-B radiation barely had any effect on the disease.