Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3206-3213.doi: 10.3864/j.issn.0578-1752.2011.15.016

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Analysis of Glucomanno-Oligosaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis

YAO  Xi-Mei, SHI  Bo, LIU  Cheng-Cheng, LIANG  Ping, LI  Jing-Mei   

  1. 1. 中国农业科学院饲料研究所
    2. 中国农业科学院作物科学研究所
  • Received:2011-03-16 Revised:2011-05-03 Online:2011-08-01 Published:2011-05-05
  • Contact: Xi-Mei YAO E-mail:yxmycc@126.com

PDF

1578

Cited

1

Abstract: 【Objective】 The objective of this study was to explore the conditions of FACE detecting glucomanno- oligosaccharides. 【Method】 Konjac glucomanno-oligosaccharides was used as the substrate, the optimal parameters were determined by investigating the effects of 3 kinds of fluorescer, the concentration of resolving gel, the conditions of derivatization reaction and sample impurities on FACE. 【Result】 The optimal conditions of FACE used in detecting glucomanno-oligosaccharides were as follows: fluorescer ANTS, concentration of resolving gel 25%, content of ANTS 2 μL, derivation reaction temperature 37℃ and derivation reaction time 16 h.【Conclusion】These findings show that FACE has a high resolution to glucomanno- oligosaccharides, and 14 kinds of components were detected by FACE, which provides an effective method for qualitative and quantitative analysis of glucomanno-oligosaccharides.

Key words: Glucomanno-oligosaccharides, FACE, ANTS, Detecting

[1]陈小兵, 丁宏标, 乔  宇. 甘露寡糖的益生作用.免疫机制与应用技术. 中国畜牧兽医, 2005, 32(8): 6-8.

Chen X B, Ding H B, Qiao Y. The probiotic function, immune mechanism and application technology of mannan-oligosaccharide. China Animal Husbandry and Veterinary Medicine, 2005, 32(8): 6-8. (in Chinese)

[2]杨艳燕, 高  尚, 王慧平, 程  珊, 马立新. 魔芋低聚糖降低糖尿病小鼠血糖和胆固醇效应的研究. 湖北大学学报: 自然科学版, 2001, 23(3): 277-279.

Yang Y Y, Gao S, Wang H P, Cheng S, Ma L X. Studies on the effect of konjac oligosaccharides on blood sugar and serum cholesterol in the diabetic mice. Journal of Hubei University: Natural Science Edition, 2001, 23(3): 277-279. (in Chinese)

[3]陈建红, 周海燕, 吴永尧. 魔芋葡甘低聚糖抗氧化性初步研究. 天然产物研究与开发, 2006, 18: 713-716.

Chen J H, Zhou H Y,Wu Y Y. Studies on the antioxidative ability of konjac oligosaccharides. Natural Product Research and Development, 2006, 18: 713-716. (in Chinese)

[4]Mirelman D, Altmann G, Eshdat Y. Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts. Journal of Clinical Microbiology, 1980, 11(4): 328-331.

[5]许牡丹, 柯  蕾. 甘露低聚糖的酶法制备与研究进展. 食品研究与开发, 2005, 26(4): 163-166.

Xu M D, Ke L. Progress in the production of mannan- oligosaccharides by β-mannanase. Food Research and Development, 2005, 26(4): 163-166. (in Chinese)

[6]Jackson P. High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides. Methods in Enzymology, 1994, 230: 250-265.

[7]Jackson P. Fluorophore-assisted carbohydrate electrophoresis: a new technology for the analysis of glycans. Biochemical Society Transactions, 1993, 1(21): 121-125.

[8]Rudd P M, Dwek R A. Rapid, sensitive sequencing of oligosaccharides from glycoproteins. Current Opinion in Biotechnology, 1997, 8(4): 488-497.

[9]Hase S, Ikenaka T, Matsushima Y. Structure analyses of oligosaccharides by tagging of the reducing end sugar with a fluorescent compound. Biochemical and Biophysical Research Communication, 1978, 85(1): 257-263.

[10]Jackson P. The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labeled with the fluorophore 8-aminonaphthalene-1, 3, 6-tirsulphonic acid. Biochemical Journal, 1990, 270: 705-713.

[11]Kunkel J P, Jamieson J C. Quantitation of fluorophore-assisted carbohydrate electrophoresis gels: comparison with high-pH change chromatography and pulsed amperometric detection. Analytical Biochemistry, 2003, 316(1): 142-146.

[12]Huang G L, Zhang H C, Wang P G. The analysis of oligosaccharides derived from different sources by fluorophore-assisted carbohydrate electrophoresis. Food Chemistry, 2007, 101: 392-396.

[13]Kooy F K, Ma M, Beeftink H H, Eggink G, Tramper J, Boeriu C G. Quantification and characterization of enzymatically produced hyaluronan with fluorophore-assisted carbohydrate electrophoresis. Analytical Biochemistry, 2009, 384(2): 329-336.

[14]孙  静, 李健军, 黄琳娟, 王仲孚. 荧光辅助糖电泳用于果胶水解产物中寡糖的分析研究. 化学学报, 2009, 67(9): 951-956.

Sun J, Li J J, Huang L J, Wang Z F. Pectic oligosaccharide analysis by fluorophore-assisted carbohydrate electrophoresis. Acta Chimica Sinica, 2009, 67(9): 951-956. (in Chinese)

[15]王绍云. 魔芋葡甘露寡糖的酶解制备及其分离纯化的研究[D]. 北京: 中国农业科学院, 2009.

Wang S Y. Study on preparation and separation of konjac glucomanno-oligosaccharides with mannose[D]. Beijing: Chinese Academy of Agricultural Sciences, 2009. (in Chinese)

[16]Sato K, Sato K, Okubo A, Yamazaki S. Optimization of derivatization with 2-aminobenzoic acid for determination of monosaccharide composition by capillary electrophoresis. Analytical Biochemistry, 1998, 262(2): 195-197.

[17]Huang Z, Prickett T, Potts M, Helm R F. The use of the 2-aminobenzoic acid tag for oligosaccharide gel electrophoresis. Carbohydrate Research, 2000, 328(1): 77-83.

[18]Jackson P, Pluskal M G, Skea W. The use of polyacrylamide gel electrophoresis for the analysis of acidic glycans labeled with the fluorophore 2-aminoacridone. Electrophoresis, 1994, 15(7): 896-902.

[19]Goubet F, Morvan C. Evidence for several galactan synthases in flax (Linum usitassimum L.) suspension-cultured cells. Plant Cell Physiology, 1993, 34(8): 1297–1303.

[20]He L P, Sato K, Abo M, Okubo A, Yamazaki S. Separation of saccharides derivatized with 2-aminobenzoic acid by capillary electrophoresis and their structural consideration by nuclear magnetic resonance. Analytical Biochemistry, 2003, 314(1): 128-134.

[21]干  信, 张迎庆. 魔芋葡甘低聚糖醛酸双酯钠的合成. 精细化工, 2002, 19(2): 105-108.

Gan X, Zhang Y Q. Synthesis of konjac oligo hydroxypropanyl glucomannuronate sulfate sodium. Fine Chemicals, 2002, 19(2): 105-108. (in Chinese)

[22]Jackson P. Polyacrylamide gel electrophoresis of reducing saccharides labeled with the fluorophore 2-aminoacridone: subpicomolar detection using an imaging system based on a cooled charge-coupled device. Analytical Biochemistry, 1991, 196(2): 236-244.

[23]Goubet F, Jackson P, Deery J M, Dupree P. Polysaccharide analysis using carbohydrate gel electrophoresis: A method to study plant cell wall polysaccharides and polysaccharide hydrolases. Analytical Biochemistry, 2002, 300(1): 53-68.

[24]Birrell H C, Camilleri P. Analysis of 2-aminoacridone-derivatized complex oligosaccharides using micellar electrokinetic capillary chromatography and laser-induced fluorescence detection. Methods in Molecular Biology, 2003, 213: 147-160.

[25]Oonuki Y, Yoshida Y, Uchiyama Y, Asari A. Application of fluorophore-assisted carbohydrate electrophoresis to analysis of disaccharides and oligosaccharides derived from glycosaminoglycans. Analytical Biochemistry, 2005, 343(2): 212-222.
[1] LIU ChunLei, WANG Juan. Genetic Variation Analysis of New Germplasm with Combined High Yield and Salt Tolerance Developed from Derivative Materials of Pokkali [J]. Scientia Agricultura Sinica, 2025, 58(20): 4117-4130.
[2] XU JiaXin, HUA Nan, WANG YongQiang, XU Hao, LIU Zhen, ZHAO XiaoRui, LI Yue, CHEN QiWei, YE Lin. Response Surface Methodology Optimization of Water, Fertilizer, and Pesticide Coupling on Chili Pepper Growth, Photosynthetic Characteristics, and Root Rot [J]. Scientia Agricultura Sinica, 2025, 58(14): 2869-2884.
[3] LÜ JinLing, YOU Ke, WANG XiaoFei, XIAO Qiang, LI WenFeng, MA Jin, YANG Qing, ZHANG JinPing, KONG HaiJiang, CHANG YunHua. Variation Characteristics and Key Influencing Factors of Near-Surface Ambient Ammonia Concentration in Typical Cropland Areas in Henan Province [J]. Scientia Agricultura Sinica, 2025, 58(1): 127-140.
[4] ZHONG JinPing, ZHENG ZiCheng, LI TingXuan, HE XiaoLing. Effect of Phosphorus Fertilizer Application Rates on the Loss of Colloidal Phosphorus on Purple Soil Slopes [J]. Scientia Agricultura Sinica, 2024, 57(8): 1547-1559.
[5] YUAN Miao, ZHOU Juan, DANG ShiZhuo, TANG XueShen, ZHANG YaHong. Functional Analysis of VvARF18 Gene in Red Globe Grape [J]. Scientia Agricultura Sinica, 2024, 57(7): 1363-1376.
[6] LI WeiJing, WANG HongYuan, XU Yang, LI Hao, ZHAI LiMei, LIU HongBin. Microbial Community Structure Characteristics at the Water-Soil Interface in Rice-Crab Co-Culture System [J]. Scientia Agricultura Sinica, 2024, 57(18): 3551-3567.
[7] ZHANG JianTao, HUANG LuSheng, LIU GuangBin, LAN YuBin, WEN Sheng. The Dynamic Wetting and Spreading Behavior of Pesticide Droplet on Rice Leaf Surface [J]. Scientia Agricultura Sinica, 2024, 57(13): 2583-2598.
[8] YANG Xi, YOU Jun, ZHOU Rong, FANG Sheng, ZHANG YanXin, WU ZiMing, WANG LinHai. Establishment of High-Throughput Detection Method for Phytic Acid Content in Sesame Seeds and Screening of Low Phytic Acid Germplasms [J]. Scientia Agricultura Sinica, 2024, 57(12): 2282-2294.
[9] WANG XiaoBin, YAN Xiang, LI XiuYing, TU Cheng. Environmental Residues of Organosiloxane-Based Adjuvants and Its Environmental Risks for Use as Agrochemical Adjuvants [J]. Scientia Agricultura Sinica, 2024, 57(1): 142-158.
[10] SUI XinYi,ZHAO XiaoGang,CHEN PengYu,LI YaLing,WEN XiangZhen. Cloning of Alternative Splice Variants of LsPHYB in Lettuce and Its Expression Patterns Under Heat Stress [J]. Scientia Agricultura Sinica, 2022, 55(9): 1822-1830.
[11] LIU ZhenRong,ZHAO WuQi,HU XinZhong,HE LiuCheng,CHEN YueYuan. Optimization of Drying Process in Oat Noodle Production [J]. Scientia Agricultura Sinica, 2022, 55(24): 4927-4942.
[12] SU YuanYuan,ZHANG DeQuan,GU MingHui,ZHANG ChunJuan,LI ShaoBo,ZHENG XiaoChun,CHEN Li. Characterization of Chilled Mutton by ATP from Different Sources [J]. Scientia Agricultura Sinica, 2022, 55(19): 3841-3853.
[13] ZHONG YanPing,SHI LiSong,ZHOU Rong,GAO Yuan,HE YanQing,FANG Sheng,ZHANG XiuRong,WANG LinHai,WU ZiMing,ZHANG YanXin. Establishment of High Efficient Extraction and Detection Technology of Sesamin and Screening of High Sesamin Germplasm [J]. Scientia Agricultura Sinica, 2022, 55(11): 2109-2120.
[14] DU JinTing,ZHANG Yan,LI Yan,WANG JiaJia,LIAO Na,ZHONG LiHuang,LUO BiQun,LIN Jiang. Optimization and Mechanism of Ultrasonic-Assisted Two-Phase Extraction of Tea Saponin [J]. Scientia Agricultura Sinica, 2022, 55(1): 167-183.
[15] YuYan YANG,YaoWen LI,Shuang XING,MinHong ZHANG,JingHai FENG. The Temperature-Humidity Index Estimated by the Changes of Surface Temperature of Broilers at Different Ages [J]. Scientia Agricultura Sinica, 2021, 54(6): 1270-1279.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd