Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3206-3213.doi: 10.3864/j.issn.0578-1752.2011.15.016

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Analysis of Glucomanno-Oligosaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis

YAO  Xi-Mei, SHI  Bo, LIU  Cheng-Cheng, LIANG  Ping, LI  Jing-Mei   

  1. 1. 中国农业科学院饲料研究所
    2. 中国农业科学院作物科学研究所
  • Received:2011-03-16 Revised:2011-05-03 Online:2011-08-01 Published:2011-05-05
  • Contact: Xi-Mei YAO E-mail:yxmycc@126.com

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Abstract: 【Objective】 The objective of this study was to explore the conditions of FACE detecting glucomanno- oligosaccharides. 【Method】 Konjac glucomanno-oligosaccharides was used as the substrate, the optimal parameters were determined by investigating the effects of 3 kinds of fluorescer, the concentration of resolving gel, the conditions of derivatization reaction and sample impurities on FACE. 【Result】 The optimal conditions of FACE used in detecting glucomanno-oligosaccharides were as follows: fluorescer ANTS, concentration of resolving gel 25%, content of ANTS 2 μL, derivation reaction temperature 37℃ and derivation reaction time 16 h.【Conclusion】These findings show that FACE has a high resolution to glucomanno- oligosaccharides, and 14 kinds of components were detected by FACE, which provides an effective method for qualitative and quantitative analysis of glucomanno-oligosaccharides.

Key words: Glucomanno-oligosaccharides, FACE, ANTS, Detecting

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