Abstract:

【Objective】 The objective of current paper is to study the characteristics of SSR markers used for distinctness and uniformity test of wheat varieties, and to screen suitable SSR molecular markers for DUS test of wheat varieties in Henan Province. 【Method】 Eighteen wheat varieties with relative closer genetic relationship to main wheat variety Zhoumai 13 and Zhoumai 16 as experimental materials and 252 pairs of SSR molecular markers were used for distinctness and uniformity analysis in the study. The main primers were screened out by stability analysis of the high discrimination power markers. Furthermore, ten wheat lines derived from Zhoumai 13 and Zhoumai 16 and forty-one widely-used varieties during the past sixty years were used to verify the discrimination power of main primers. 【Result】 The results showed that the discrimination power of SSR molecular markers to wheat varieties had significant positive correlations to polymorphism loci. Six main primers with preferable distinctness and uniformity performance (Xwmc679, Xwmc574, Xgwm497, Xwmc388, Xwmc500 and Xgwm637) were screened from 252 SSR primers, which could distinguish completely all the sixty-nine experimental wheat materials used in the study. 【Conclusion】 Discrimination power of SSR molecular markers to wheat varieties had significant positive correlation to polymorphism loci. Six SSR primers Xwmc679, Xwmc574, Xgwm497, Xwmc388, Xwmc500 and Xgwm637 showing excellent discrimination power and stability could be used for distinctness and uniformity identification of wheat varieties in Henan Province.

Key words: wheat varieties, DUS testing, SSR