Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (12): 2608-2614 .doi: 10.3864/j.issn.0578-1752.at-2005-5771

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Genetic Relationships of Antheraea pernyi Cultivars based on RAPD Markers

YanQun Liu,Cheng Lu,Li Qin,ZhongHuai Xiang   

  1. 南开大学
  • Received:2005-07-06 Revised:1900-01-01 Online:2006-12-10 Published:2006-12-10
  • Contact: YanQun Liu

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Abstract: The genetic relationships of 68 A. pernyi cultivars were analyzed with random amplified polymorphic DNA (RAPD). A total of 296 DNA fragments were amplified with 33 primers selected among A. pernyi cultivars, 269 of which (90.88%) were polymorphic. Only nine (12.68%) of A. pernyi cultivars used had one or more cultivar-specific markers. The value of pairwise genetic distance were from 0.120 to 0.324, and most of them were in the range of 0.200 to 0.300. We found 99.05% of the cultivar pairs with pairwise genetic distance less than 0.300 and 0.95% of cultivar pairs with pairwise genetic distance exceeding 0.300. Generally, there was great genetic similarity among A. pernyi cultivars. A dendrogram was constructed by Neighbor-Joining method. The A. pernyi cultvars used were classified into 6 genetic groups. Cultivargroupings were closely related to their geographic distribution in the present.

Key words: Antheraea pernyi, Cultivar, RAPD, Genetic relationship

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