Abstract: Abstract: The antioxidative activity of Ganoderma lucidum peptides (GLP) in vitro test was studied. The reagent kits were used to determine the content of malondialdehyde (MDA). The auto-hemolysis of mice red blood cells (RBC) and the swelling of mice liver mitochondria were observed by means of spectrophotometer. The results showed that the inhibiting rate of 0.25 mg·ml-1 GLP on the auto-hemolysis of mice RBC was 62.3% and the inhibiting rate on the MDA formation in mice RBC was 91.2%. 0.35 mg ml-1 GLP could extremely significantly prevent the MDA formation in mice liver homogenate ( p<0.01) with or without free radicals inducers (Fe2+or H2O2). They had the near inhibiting rate-around 60%. When 1.00 mg·ml-1 GLP was added, the inhibiting rate on the swelling of mice liver mitochondria was 90.5% and the inhibiting rate on the MDA formation in mice liver mitochondria was 65.4% in a dose-dependent manner. Conclusion: GLP was a good antioxidant in vitro test.

Key words: Ganoderma lucidum, peptides, antioxidation