Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (12): 2577-2581 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Studies on The Developmental Patterns of IGF-Ⅰand IGF-ⅠR Gene Expression in Liver Tissue of Lamb

  

  1. 安徽农业大学动物科技学院
  • Received:2005-06-28 Revised:2005-12-08 Online:2006-12-10 Published:2006-12-10

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Abstract: Abstract：In present experiment, A Real time polymerase chain reaction (PCR) was applied to investigate the development patterns of IGF-Ⅰ, IGF-ⅠR mRNA expression in liver tissue of lamb, and GAPDH internal standards were used as control. Fifty-fourth of XJXM and HSK sheep were sampled at 2,30,60,90,120 days of age respectively (Only there have sixth of 120 days of age in XJXM sheep). The results showed that (1) IGF-ⅠmRNA expression levels in liver tissue of XJXM and HSK sheep follow the same developmental pattern. The expression levels of IGF-ⅠmRNA in liver tissue increased firstly and declined thereafter gradually. The expression of IGF-ⅠmRNA in HSK sheep was increased gradually between 2 days of age and 60 days of age, and declined gradually after 60 days of age. The expression of IGF-ⅠmRNA in XJXM sheep was increased gradually between 2 days of age and 90 days of age, and declined gradually after 90 days of age. The expression of IGF-ⅠmRNA in HSK sheep was higher than that in XJXM sheep at 30 and 60 days of age (P<0.05), and at 90 days of age (P<0.01). (2) IGF-ⅠR mRNA expression levels in liver tissue of XJXM and HSK sheep follow the same developmental pattern. The expression levels of IGF-ⅠmRNA in liver tissue declined gradually. The expression of IGF-ⅠmRNA in HSK sheep was highest at 2 days of age and declined gradually thereafter. The expression of IGF-ⅠR mRNA in HSK sheep at 2 days of age was higher than that at the other days of age(P<0.05). The expression of IGF-ⅠmRNA in XJXM sheep was highest at 2 days of age and declined gradually thereafter. The expression of IGF-ⅠR mRNA in XJXM sheep at 2 days of age was higher than that at the other days of age(P<0.05). The expression of IGF-ⅠR mRNA in HSK sheep was lower than that in XJXM sheep at 2 and 90 days of age (P<0.01). The result show that the IGF-Ⅰand IGF-ⅠR mRNA expression in liver tissue follow specific developmental pattern respectively. Level of IGF-ⅠR mRNA expression does not depend on that of IGF-ⅠmRNA expression.

Key words: Lamb, (Insulin like growth factorⅠ) IGF-Ⅰ, (Insulin like growth factorⅠreceptor) IGF-ⅠR, Liver tissue, Real time polymerase chain reaction

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