代乳粉添加单宁酸对7—28日龄湖羊羔羊胃肠道发育的影响

doi:10.3864/j.issn.0578-1752.2019.21.019

Abstract

Abstract:

【Objective】 This study was conducted to investigate the effects of tannic acid supplementation to milk replacer on the development of gastrointestinal tract of 7 to 28 day-old Hu lambs. 【Method】 Thirty 7 day-old Hu male lambs were chosen and divided into 2 groups, fifteen lambs in each group and each lamb as a duplication. Lambs were fed milk replacer with or without 0.2% tannic acid, respectively. The test lasted 21 days. Eight lambs were selected from each group randomly and slaughtered at 28 day-old. The weights of the compound stomach and the intestinal tract with and without content, and lengths of the intestinal tract were measured, then the relative quality and length were calculated. While the tissue samples from fundus gland region of the abomasum, the middle part of duodenum, jejunum and ileum were fixed in paraformaldehyde to analyse the histomorphology, and the apoptotic rate of intestinal epithelial cells as well. And the mRNA expression of occludin, ZO-1 and claudin 1 protein of duodenum, jejunum and ileum mucosa were measured.【Result】 The results showed that except relative weights of duodenum (% body weight, P=0.012; % intestinal tract weight, P=0.034; % gastrointestinal tract weight, P=0.017), relative length of jejunum and colon (% intestinal tract length, P=0.030, P=0.004), and content of colon (% body weight, P=0.039), the relative weights (% body weight, % stomach weight, % intestinal tract weight, and % gastrointestinal tract weight), relative lengths (% intestinal tract length), content of stomach and intestinal tract (% body weight, % stomach content weight, % intestinal tract content weight, and % gastrointestinal tract content weight), and mRNA expression of claudin 1 protein in intestinal tract of lambs were not affected by tannic acid (P＞0.05). However, tannic acid elongated the muscular thickness of duodenum and decreased the villus width of lamb duodenum significantly (P=0.013,P=0.001), and up-regulated mRNA expression of ZO-1 protein of jejunum (P=0.003). And there was a tendency that tannic acid up-regulated mRNA expression of occludin protein of jejunum (P=0.077), and decreased villus width and crypt depth of jejunum and the apoptotic rate of jejunum and ileum cells (P=0.073, P=0.062, P=0.097, P=0.052). 【Conclusion】 In conclusion, tannic acid decreased the relative weights of duodenum and relative length of jejunum of 7-28 day-old Hu lambs, but improved the barrier function of intestinal tract via elongating the muscular thickness of duodenum, up-regulating the mRNA expression of ZO-1 and occludin protein of jejunum, and decreasing the crypt depth of jejunum and the apoptotic rate of jejunum and ileum cells.

Key words: tannic acid, lamb, milk replacer, gastrointestinal tract, development

ZHENG Chen,LI FaDi,LI Fei,ZHOU JuWang,DUAN PengWei,LIU HuiHui,FAN HaiMiao,ZHU WeiLi,LIU Ting. Effects of Tannic Acid Addition in Milk Replacer on Development of Gastrointestinal Tract of 7 to 28 Days Old Hu Lambs[J].Scientia Agricultura Sinica, 2019, 52(21): 3924-3933.

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Figures/Tables 6

Table 1

Table 2

Effects of tannic acid on relative quality of gastrointestinal tract of lamb"

项目 Item		对照组 Control	单宁酸组 Tannic acid	SEM	P值 P value
胃室 Stomach
瘤胃 Rumen	%活体质量 % body weight	0.54	0.55	0.02	0.856
	%全胃质量 % the quality of total stomach	29.70	31.03	1.10	0.564
	%全胃肠质量 % the quality of total gastrointestinal tract	6.69	7.50	0.34	0.247
网胃 Reticulum	%活体质量 % body weight	0.16	0.16	0.01	0.864
	%全胃质量 % the quality of total stomach	9.16	9.03	0.35	0.859
	%全胃肠质量 % the quality of total gastrointestinal tract	2.04	2.19	0.09	0.426
瓣胃 Omasum	%活体质量 % body weight	0.08	0.08	0.01	0.659
	%全胃质量 % the quality of total stomach	4.08	4.59	0.32	0.450
	%全胃肠质量 % the quality of total gastrointestinal tract	0.92	1.14	0.10	0.304
皱胃 Abomasum	%活体质量 % body weight	1.04	1.00	0.05	0.683
	%全胃质量 % the quality of total stomach	57.05	55.34	1.23	0.506
	%全胃肠质量 % the quality of total gastrointestinal tract	12.77	13.40	0.40	0.448
肠道 Intestinal tract
十二指肠 Duodenum	%活体质量 % body weight	0.20^a	0.15^b	0.01	0.012
	%全肠质量 % the quality of total intestinal tract	3.21^a	2.69^b	0.12	0.034
	%全胃肠质量 % the quality of total gastrointestinal tract	2.48^a	2.04^b	0.10	0.017
空肠Jejunum	%活体质量 % body weight	4.00	3.59	0.15	0.162
	%全肠质量 % the quality of total intestinal tract	63.93	63.29	0.73	0.675
	%全胃肠质量 % the quality of total gastrointestinal tract	49.63	47.98	0.79	0.317
回肠 Ileum	%活体质量 % body weight	0.15	0.14	0.01	0.878
	%全肠质量 % the quality of total intestinal tract	2.35	2.52	0.17	0.642
	%全胃肠质量 % the quality of total gastrointestinal tract	1.83	1.91	0.14	0.772
盲肠 Cecum	%活体质量 % body weight	0.35	0.29	0.02	0.072
	%全肠质量 % the quality of total intestinal tract	5.54	5.16	0.25	0.456
	%全胃肠质量 % the quality of total gastrointestinal tract	4.30	3.90	0.19	0.321
结肠 Colon	%活体质量 % body weight	1.18	1.11	0.05	0.522
	%全肠质量 % the quality of total intestinal tract	18.85	19.64	0.53	0.477
	%全胃肠质量 % the quality of total gastrointestinal tract	14.61	14.88	0.40	0.750
直肠 Rectum	%活体质量 % body weight	0.38	0.37	0.02	0.755
	%全肠质量 % the quality of total intestinal tract	6.11	6.70	0.34	0.407
	%全胃肠质量 % the quality of total gastrointestinal tract	4.73	5.06	0.24	0.517

Table 2

Table 3

Table 4

Effects of tannic acid on relative quality of gastrointestinal tract content of lambs"

项目 Item		对照组 Control	单宁酸组 Tannic acid	SEM	P值 P value
胃室内容物 Stomach content
瘤胃内容物 Rumen content	%活体质量 % body weight	1.44	1.75	0.32	0.648
	%全胃内容物质量 % the quality of total stomach content	26.96	37.02	4.58	0.287
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	17.32	22.73	3.15	0.410
网胃内容物 Reticulum content	%活体质量 % body weight	0.27	0.20	0.06	0.553
	%全胃内容物质量 % the quality of total stomach content	4.99	3.39	0.91	0.405
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	3.51	2.13	0.71	0.358
瓣胃内容物 Omasum content	%活体质量 % body weight	0.01	0.01	0.0005	0.776
	%全胃内容物质量 % the quality of total stomach content	0.23	0.31	0.03	0.191
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	0.14	0.17	0.01	0.333
皱胃内容物 Abomasum content	%活体质量 % body weight	3.43	2.27	0.30	0.053
	%全胃内容物质量 % the quality of total stomach content	68.58	60.65	4.65	0.412
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	43.32	34.89	3.00	0.168
肠道内容物 Intestinal tract content
十二指肠内容物 Duodenum content	%活体质量 % body weight	0.15	0.09	0.02	0.111
	%全肠内容物质量 % the quality of total intestinal tract content	7.25	3.46	1.44	0.198
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	1.99	1.47	0.29	0.392
空肠内容物 Jejunum content	%活体质量 % body weight	1.26	1.10	0.18	0.670
	%全肠内容物质量 % the quality of total intestinal tract content	39.64	44.17	2.75	0.429
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	14.33	18.16	1.91	0.332
回肠内容物 Ileum content	%活体质量 % body weight	0.08	0.04	0.02	0.218
	%全肠内容物质量 % the quality of total intestinal tract content	1.75	1.77	0.32	0.978
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	0.80	0.75	0.16	0.904
盲肠内容物 Cecum content	%活体质量 % body weight	0.43	0.33	0.08	0.554
	%全肠内容物质量 % the quality of total intestinal tract content	12.85	13.20	1.82	0.928
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	4.90	4.89	0.64	0.991
结肠内容物 Colon content	%活体质量 % body weight	1.08^a	0.57^b	0.13	0.039
	%全肠内容物质量 % the quality of total intestinal tract content	33.28	25.72	2.57	0.148
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	12.53	10.96	1.42	0.596
直肠内容物 Rectum content	%活体质量 % body weight	0.17	0.23	0.04	0.449
	%全肠内容物质量 % the quality of total intestinal tract content	6.10	11.68	2.09	0.192
	%全胃肠内容物质量 % the quality of total gastrointestinal tract content	2.08	4.70	0.87	0.137

Table 4

Table 5

Table 6

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营养成分 Ingredient	含量 Concentration
干物质 Dry matter	95.68
粗蛋白质 Crude protein	24.89
粗脂肪 Ether extract	17.50
粗纤维 Crude fiber	2.16
粗灰分 Crude ash	5.81
钙 Calcium	1.02
总磷 Total phosphorus	0.59

项目Item	对照组Control	单宁酸组 Tannic acid	SEM	P值 P value
皱胃 Abomasum
黏膜厚度 Mucosa thickness (μm)	333.36	343.82	8.54	0.605
肌层厚度 Muscular thickness (μm)	548.48	278.36	52.15	0.810
十二指肠 Duodenum
绒毛高度 Villus height (μm)	361.99	425.00	21.54	0.150
绒毛宽度 Villus width (μm)	80.04^a	54.80^b	4.43	0.001
隐窝深度 Crypt depth (μm)	148.87	156.30	8.10	0.665
肌层厚度 Muscular thickness (μm)	161.93^b	208.41^a	10.01	0.013
绒隐比 V / C	2.57	2.76	0.13	0.505
空肠Jejunum
绒毛高度 Villus height (μm)	431.59	378.90	19.85	0.194
绒毛宽度 Villus width (μm)	71.13	64.74	1.79	0.073
隐窝深度 Crypt depth (μm)	173.48	143.65	8.05	0.062
肌层厚度 Muscular thickness (μm)	157.73	146.10	7.41	0.451
绒隐比 V / C	2.59	2.79	0.07	0.154
回肠 Ileum
绒毛高度 Villus height (μm)	373.81	383.77	29.23	0.883
绒毛宽度 Villus width (μm)	68.91	78.12	2.76	0.108
隐窝深度 Crypt depth (μm)	176.96	207.92	11.78	0.228
肌层厚度 Muscular thickness (μm)	274.31	302.91	46.72	0.792
绒隐比 V / C	2.19	1.85	0.12	0.194

项目Item	对照组Control	单宁酸组 Tannic acid	SEM	P值 P value
十二指肠 Duodenum
Occludin	0.86	1.16	0.10	0.124
ZO-1	0.91	1.07	0.08	0.317
Claudin 1	0.91	0.94	0.09	0.850
细胞凋亡 Apoptosis (%)	11.27	7.90	2.97	0.595
空肠Jejunum
Occludin	0.82	1.39	0.16	0.077
ZO-1	0.71^b	1.29^a	0.11	0.003
Claudin 1	0.97	1.10	0.25	0.800
细胞凋亡 Apoptosis (%)	21.09	3.99	5.13	0.097
回肠 Ileum
Occludin	0.95	1.02	0.10	0.744
ZO-1	0.88	1.11	0.10	0.249
Claudin 1	0.80	1.00	0.16	0.544
细胞凋亡 Apoptosis (%)	47.29	20.76	7.00	0.052

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Effects of Tannic Acid Addition in Milk Replacer on Development of Gastrointestinal Tract of 7 to 28 Days Old Hu Lambs

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项目Item	对照组Control	单宁酸组 Tannic acid	SEM	P值 P value
十二指肠 Duodenum	2.28	2.08	0.11	0.361
空肠Jejunum	81.90^a	80.33^b	0.37	0.030
回肠 Ileum	0.84	0.84	0.06	0.990
盲肠 Cecum	0.90	0.93	0.05	0.848
结肠 Colon	11.84^b	13.56^a	0.33	0.004
直肠 Rectum	2.23	2.26	0.13	0.886