Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (05): 1058-1063 .doi: 10.3864/j.issn.0578-1752.at-2005-5332

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Cloning Full Length of Ramie Caffeoyl-CoA 3-O-Methyltransferase cDNA and Sequence Analysis

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  • Received:2005-03-29 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10

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Abstract: 【Objective】 The full length of ramie(Boehmeria nivea) caffeoyl-CoA 3-O-methyltransferase cDNA was cloned. 【Method】 RACE (Rapid Amplification of cDNA Ends) method was used to analyze the sequence by ClustaW 1.82 software on line.【Result】 The full length of ramie caffeoyl-CoA 3-O-methyltransferase cDNA was cloned for the first time (GenBank accession number: AY 651026). The result of domain analyze showed that it is a member of O-methyltransferases family. Compared ramie mRNA sequence, mRNA CDS sequence and putative amino acid sequence CCoAOMT to other higher plants, phylogenetic tree of CCoAOMT was generated by multiple alignment. The result displayed that mRNA level of ramie can not be clustered into any other plant, but the result showed that ramie and Zea mays (ZMA242980, ZMA242981) have higher homology in mRNA CDS than other higher plants, while the amino acid sequence of ramie and populus (AJ224894, AJ224896, PTU27116) has higher homology than other plants.【Conclusion】Ramie (Boehmeria nivea) caffeoyl-CoA 3-O-methyltransferase cDNA has homology with that of other plants.

Key words: Ramie(Boehmeria nivea ), caffeoyl-CoA 3-O-methyltransferase, full length cDNA, gene cloning, Sequence analysis

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