Scientia Agricultura Sinica ›› 2004, Vol. 37 ›› Issue (10): 1571-1574 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Determination of Avermectin Residue in Earthworms by High Performance Liquid Chromatography

,   

  1. 中国农业大学动物医学院
  • Received:2003-12-04 Revised:1900-01-01 Online:2004-10-20 Published:2004-10-20

PDF

764

Cited

2

Abstract: A method for determination of avermectin B1a in earthworms was established by high performance liquid chromatography (HPLC) with fluorescence detector. Homogenized earthworms sample was extracted by acetonitrile, and cleaned up on alumina B cartridge and C18 solid-phase cartridge, followed by derivatization with 1-methylimidazole (1-MIZ) and trifluoroacetic anhydride (TFAA), and analyzed by HPLC with fluorescence detector set at 365 nm excitation and 475 nm emission wavelengths. The limit of detection of the method is 0.5 ng·g-1. The average recoveries of avermectin were 104.8%, 86,6% and 83.0%, respectively in earthworm samples fortified at 5, 50, 500 ng·g-1 and the Coefficient of variation ranged from 2.99% to 7.34%.

Key words: Avermectin, Residue, HPLC, Earthworm

[1] WANG YuTai,XU ZhiFan,LIU Jie,ZHONG GuoHua. Preparation and Application of Indoxacarb Degrading Bacteria Immobilized Sodium Alginate Microspheres [J]. Scientia Agricultura Sinica, 2022, 55(5): 920-931.
[2] ZHANG XueLin, WU Mei, HE TangQing, ZHANG ChenXi, TIAN MingHui, LI XiaoLi, HOU XiaoPan, HAO XiaoFeng, YANG QingHua, LI ChaoHai. Effects of Crop Residue Decomposition on Soil Inorganic Nitrogen and Greenhouse Gas Emissions from Fluvo-Aquic Soil and Shajiang Black Soil [J]. Scientia Agricultura Sinica, 2022, 55(4): 729-742.
[3] LI JiaYan,SUN LiangJie,MA Nan,WANG Feng,WANG JingKuan. Carbon and Nitrogen Fixation Characteristics of Maize Root and Straw Residues in Brown Soil Under High and Low Fertility [J]. Scientia Agricultura Sinica, 2022, 55(23): 4664-4677.
[4] ZHANG JinRui,REN SiYang,DAI JiZhao,DING Fan,XIAO MouLiang,LIU XueJun,YAN ChangRong,GE TiDa,WANG JingKuan,LIU Qin,WANG Kai,ZHANG FuSuo. Influence of Plastic Film on Agricultural Production and Its Pollution Control [J]. Scientia Agricultura Sinica, 2022, 55(20): 3983-3996.
[5] HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[6] HUANG Ming,WU JinZhi,LI YouJun,FU GuoZhan,ZHAO KaiNan,ZHANG ZhenWang,YANG ZhongShuai,HOU YuanQuan. Effects of Tillage Practices and Nitrogen Fertilizer Application Rates on Grain Yield, Protein Content in Winter Wheat and Soil Nitrate Residue in Dryland [J]. Scientia Agricultura Sinica, 2021, 54(24): 5206-5219.
[7] MA Yue,TIAN Yi,YUAN AiJing,WANG HaoLin,LI YongHua,HUANG TingMiao,HUANG Ning,LI Chao,DANG HaiYan,QIU WeiHong,HE Gang,WANG ZhaoHui,SHI Mei. Response of Wheat Yield and Protein Concentration to Soil Nitrate in Northern Wheat Production Region of China [J]. Scientia Agricultura Sinica, 2021, 54(18): 3903-3918.
[8] HuiMin HU,XueFeng PAN,Heng YANG,Chen CHEN,YinJi CHEN. Wheat Gluten, Gliadins and Glutenin Content Changes During Germination Based on the Methods of R5 ELISA and RP-HPLC [J]. Scientia Agricultura Sinica, 2020, 53(6): 1247-1255.
[9] XU Ying,YAN ChangYan,YANG WeiCong,ZHANG YunXiao,YU Yang,HUANG XianHui. Pharmacokinetics of Chlortetracycline Microspheres in Pigs [J]. Scientia Agricultura Sinica, 2020, 53(19): 4083-4091.
[10] LI XiaoBei,ZHAO XiaoYan,LI JianYing,CHEN Lei,ZHOU ChangYan,HE XiangWei. Residue Behavior and Dietary Intake Risk Assessment of Imidaclothiz in Pakchoi (Brassica chinensis L.) [J]. Scientia Agricultura Sinica, 2020, 53(17): 3587-3596.
[11] WeiSong ZHAO,QingGang GUO,SheZeng LI,YaJiao WANG,XiuYun LU,PeiPei WANG,ZhenHe SU,XiaoYun ZHANG,Ping MA. Control Efficacy of Broccoli Residues on Cotton Verticillium Wilt and Its Effect on Soil Bacterial Community at Different Growth Stages [J]. Scientia Agricultura Sinica, 2019, 52(24): 4505-4517.
[12] ZHANG XueLin, ZHOU YaNan, LI XiaoLi, HOU XiaoPan, AN TingTing, WANG Qun. Effects of Nitrogen Fertilizer on Crop Residue Decomposition and Nutrient Release Under Lab Incubation and Field Conditions [J]. Scientia Agricultura Sinica, 2019, 52(10): 1746-1760.
[13] LI ZhanSheng, LIU YuMei, FANG ZhiYuan, YANG LiMei, ZHUANG Mu, ZHANG YangYong, Lü HongHao. Full Length Cloning, Expression and Correlation Analysis of P450 CYP79F1 Gene with Sulforaphane Content in Different Broccoli Organs [J]. Scientia Agricultura Sinica, 2018, 51(12): 2357-2367.
[14] LAN Yue, HU Yue, WANG Yan, Guo YanZhen, ZHAO HengKe, HE Lin, QIAN Kun. Preparation of Acetochlor Microcapsules by Interfacial Polymerization and the Environmental Behavior and Control Efficacy [J]. Scientia Agricultura Sinica, 2017, 50(14): 2739-2747.
[15] LIU Yu-ting, WU Ming-yang, JIN Yan-ling, SHEN Wei-liang, FANG Yang, ZHAO Hai. Lactic Acid Fermentation by Lactobacillus rhamnosus from Sweet Potato Residue [J]. Scientia Agricultura Sinica, 2016, 49(9): 1767-1777.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd