Scientia Agricultura Sinica ›› 2004, Vol. 37 ›› Issue (10): 1564-1570 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Molecular Cloning and Sequence Analysis of IGF-I from Triangular Bream (Megalobrama terminalis)

  

  1. 浙江大学动物科技学院
  • Received:2003-05-30 Revised:2004-05-12 Online:2004-10-20 Published:2004-10-20

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Abstract: The insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of triangular bream (Megalobrama terminalis) (GenBank No.AY247412) was cloned for the first time from liver by RT-PCR .The nucleotide Sequence analysis showed that the triangular bream IGF-Ⅰ cDNA was consisted of 486 nucleotides and encoded 117 amino acids including B,C,A,D and E five domains. Analysis of E domain indicated that cloned triangular bream IGF-Ⅰbelonged to IGF-ⅠEa-2 subtype. Identity analysis showed that the IGF-Ⅰnucleotide sequence shared 99.8% homology with that of bluntnose bream, 88.8% homology with that of grass carp, 85.8% homology with that of common carp. The pre-IGF-Ⅰamine acid sequence shared 99.4% homology with that of bluntnose bream, 88.8% homology with that of grass carp, 85.4% homology with that of common carp. In the Cyprinus Carpio, the more high homology of nucleotide sequence and amino acid sequence in IGF-Ⅰshowed that the fishes have a closer relationship. These results could provide basic data for the research on triangular bream germplasm and the development and utilization of biological feed additives.

Key words: Triangular bream (Megalobrama terminalis), IGF-Ⅰ, Molecular cloning, Sequence analysis

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