Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (7): 1486-1491 .doi: 10.3864/j.issn.0578-1752.at-2005-6447

• HORTICULTURE • Previous Articles     Next Articles

Identification of 16SrDNA and Research on Acylated Homoserine Lactones Produced by Pseudomomas Isolated from the Fish

  

  1. 南京农业大学食品科技学院
  • Received:2005-12-02 Revised:1900-01-01 Online:2007-07-10 Published:2007-07-10

PDF

1058

Abstract: Three Gram-negative bacteria were isolated from the commercial fresh fish and considered to be the pseudomonas group , an important microorganism that caused the fish spoilage ,by analysis of 16SrDNA sequence.The characteristics (Protase ,siderophore(s) ,formation biofilm) involved food spoilage were assayed . N-acyl-homoserine lactones (AHLs) were the important quorum sensing molecules and regulated the expression of many characteristics in Gram-negative .Three isolates produced the AHLs incolved in quorum sensing.Thin-layer chromatography revealed presence of two AHLs in extract from the Pseudomonas fluorescens FLM05-1and Pseudomonas fluorescens FLM05-2 and the main signal molecule was N-3-oxo-octanoyl-homoserine lactone.Production siderophore , protase of FLM05-2 were significantly reduced by co-incubation with AHL degrader Pseudomonas fluorescens P3(pME6863). It was suggested that QS play an important role in the food spoilage. This was first study of production AHLs ,quorum sensing signal molecule by the pseudomonas , and new strategies were provided for food preservation based on quorum sensing

Key words: Pseudomonas, Biofilm, siderophore(s), Protase, N-acyl- homoserine lactones, Quorum sensing, Food spoilage

[1] LIAO Kai, LI Xin, SHI YanXia, XIE XueWen, LI Lei, FAN TengFei, WANG ShaoHui, LI BaoJu, CHAI ALi. Effect of Different Ventilation Methods in Plastic Sheds on the Spread of Cucumber Bacterial Angular Leaf Spot [J]. Scientia Agricultura Sinica, 2025, 58(10): 1934-1946.
[2] WANG NanWei, LI LiLi, CHEN KaiFeng, ZHOU ZhouPing, PAN Peng, GUAN Jin, XU ChengGang, LIAO Ming, ZHANG JianMin. STM2503 Regulates Biofilm Formation and Stress Adaptability of S. Typhimurium [J]. Scientia Agricultura Sinica, 2024, 57(16): 3294-3304.
[3] LUO WanZhen, WANG Dan, QI HongYue, WANG Tong, LIU Zheng, TIAN Li, DAI XiaoFeng, CHEN JinYin, MIJITI Maihemuti. Identification of Antagonistic Bacterium Strain KRS022 and Its Inhibition Effect on Verticillium dahliae [J]. Scientia Agricultura Sinica, 2023, 56(4): 649-664.
[4] WU YaJie, TAN Song, CHEN YuPing, NIU AJuan, LIU YuXin, WANG GuangYu, XU XingLian, QIU WeiFen. Effects of nuoB on the Biofilm Formation and Cellular Metabolism of Meat-Borne Pseudomonas fragi During Chilled Storage [J]. Scientia Agricultura Sinica, 2023, 56(11): 2172-2185.
[5] WANG YuTai,XU ZhiFan,LIU Jie,ZHONG GuoHua. Preparation and Application of Indoxacarb Degrading Bacteria Immobilized Sodium Alginate Microspheres [J]. Scientia Agricultura Sinica, 2022, 55(5): 920-931.
[6] TANG ZiYun,HU JianXin,CHEN Jin,LU YiXing,KONG LingLi,DIAO Lu,ZHANG FaFu,XIONG WenGuang,ZENG ZhenLing. Relationship Between Biofilm Formation and Molecular Typing of Staphylococcus aureus from Animal Origin [J]. Scientia Agricultura Sinica, 2022, 55(3): 602-612.
[7] ZHANG JinLong,ZHAO ZhiBo,LIU Wei,HUANG LiLi. The Function of Key T3SS Effectors in Pseudomonas syringae pv. actinidiae [J]. Scientia Agricultura Sinica, 2022, 55(3): 503-513.
[8] SHA YueXia, HUANG ZeYang, MA Rui. Control Efficacy of Pseudomonas alcaliphila Strain Ej2 Against Rice Blast and Its Effect on Endogenous Hormones in Rice [J]. Scientia Agricultura Sinica, 2022, 55(2): 320-328.
[9] WANG GuangYu,LI Qing,TANG WenQian,WANG HuHu,XU XingLian,QIU WeiFen. Effects of nuoB on Physiological Properties of Pseudomonas fragi and Its Spoilage Potential in Chilled Chicken [J]. Scientia Agricultura Sinica, 2021, 54(8): 1761-1771.
[10] CHEN ChaoXi,LI YuHan,TAN Min,WANG Lu,HUANG ZhiHong. Biofilm-Forming Phenotype, Antibacterial Resistance Genes, Integrase Genes and Virulence Genes Detection of Escherichia coli Isolated from Yaks and Tibetan Pigs in Northwest Sichuan Plateau [J]. Scientia Agricultura Sinica, 2021, 54(23): 5144-5162.
[11] ZHANG AiJing,LI LinQiong,WANG PengJie,GAO YuLong. Effects of Heat Stress on Cell Membrane and Membrane Protein of Escherichia coli [J]. Scientia Agricultura Sinica, 2020, 53(5): 1046-1057.
[12] YANG Jun,CHU PinPin,SONG Shuai,CAI RuJian,YANG DongXia,BIAN ZhiBiao,GOU HongChao,LI Yan,JIANG ZhiYong,LI ChunLing,YAN He. Construction of lpxM Gene Deletion Strain of Haemophilus parasuis and It's Some Biological Characteristics [J]. Scientia Agricultura Sinica, 2020, 53(16): 3394-3403.
[13] HuaFei ZHOU,HongFu YANG,KeBing YAO,YiQing ZHUANG,ZhaoLin SHU,ZhiYi CHEN. FliZ Regulated the Biofilm Formation of Bacillus subtilis Bs916 and Its Biocontrol Efficacy on Rice Sheath Blight [J]. Scientia Agricultura Sinica, 2020, 53(1): 55-64.
[14] CAI LinLin, HU HaiJing, YI XiaoKun, WANG HuHu, XU XingLian, PENG Bin. Lethal Effect of Meat-Borne Pseudomonas fluorescens to Acidic Electrolyzed Water [J]. Scientia Agricultura Sinica, 2019, 52(9): 1614-1623.
[15] GAO Shi-gang, ZENG Rong, XU Li-hui, LUO Jin-yan, CHEN Lei, DAI Fu-ming. Triplex PCR Detection for Corynespora cassiicola,Colletotrichum orbiculare and Pseudomonas syringae pv. lachrymans [J]. Scientia Agricultura Sinica, 2016, 49(16): 3119-3129.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd