外源油菜素内酯减轻SPVD对甘薯块根萌芽的抑制作用

doi:10.3864/j.issn.0578-1752.2025.22.006

Abstract

Abstract:

【Objective】This study aimed to elucidate the mechanisms by which exogenous brassinolide (BR) alleviates the inhibitory effects of sweet potato virus disease (SPVD) complex on storage roots germination in sweet potato, so as to provide the theoretical insights for mitigating SPVD impacts on sweet potato seedling production. 【Method】SPVD-infected ‘Jishu 25’ storage roots were subjected to seed soaking treatments with varying BR concentrations (0.1, 0.5 and 1.0 μmol·L^-1) or brassinazole (BRZ, 0.1 μmol·L^-1). Germination kinetics, soluble sugar content, antioxidant enzyme activities (SOD, POD and CAT), reactive oxygen species (ROS) accumulation, malondialdehyde (MDA) content, and expression levels of BR biosynthesis genes (CYP85A1 and CYP90D1) were quantified to characterize BR/BRZ-induced alterations in cellular redox homeostasis and transcriptional regulation. 【Result】SPVD significantly suppressed storage roots germination. 7 d, 14 d, and 28 d after seedling cultivation, the storage roots of sweet potato infected with SPVD germinate later, and the number and quality of seedlings were reduced. During germination initiation stage (7-14 d): Lower BR concentrations (0.1-0.5 μmol·L^-1) enhanced quality of germination, elevated soluble sugar content, upregulated antioxidant enzyme activities, reduced ROS and MDA accumulation, and stimulated CYP85A1 and CYP90D1 expression. BRZ treatment exacerbated germination inhibition, suppressed antioxidant defenses, and increased oxidative damage. During the period of rapid germination (21-28 d), 0.1 μmol·L^-1 BR sustained germination promotion, reduced soluble sugar content, maintained high antioxidant activity, minimized oxidative stress, and further amplified BR biosynthetic genes expression. BRZ consistently impaired germination, antioxidant capacity, and BR genes expression. 【Conclusion】The germination of sweet potato storage roots infected with SPVD was significantly inhibited. The appropriate concentration of BR (0.1 μmol·L^-1) significantly alleviated the inhibition of SPVD on sweet potato storage roots germination by activating the antioxidant defense system, optimizing the dynamic balance of sugar metabolism, and enhancing the expression of endogenous BR synthesis genes.

Key words: sweet potato, storage root germination, brassinolide, SPVD

DU TaiFeng, ZHOU YuanYuan, QIN Zhen, LI AiXian, WANG QingMei, ZHANG LiMing, HOU FuYun. Exogenous Brassinolide Alleviates the Inhibitory Effect of SPVD on Sweet Potato Storage Roots Germination[J].Scientia Agricultura Sinica, 2025, 58(22): 4628-4637.

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References 45

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引物名称 Primer name	引物序列 Primer sequence (5′-3′)
IbActin-F	CTGGTGTTATGGTTGGGATGG
IbActin-R	GGGGTGCCTCGGTAAGAAG
SPFMV-F	GACTGATATGAGTCTTGCGCGRTATGCG
SPFMV-R	TGTGCCTCTCCGTATCYTCTTCTTGCGT
SPCSV-F	CCCAACGTGTTTATCTATTACTAAGAGTGG
SPCSV-R	AATACTGGGGAGCTATCTTACGTTTGA

引物名称 Primer name	引物序列 Primer sequence (5′-3′)
CYP85A1-F	GCAGCTATCCGAGGAGCATT
CYP85A1-R	GCTGTGTTCTGAGCCTAGCA
CYP90D1-F	ATCTCCCTTTGGGCAACCTG
CYP90D1-R	TCTCTGGGCGGTCAGAGTAA

育苗时间 Seedling days	处理Treatment	出苗数量 Germination quantity (plant/kg)	出苗质量 Germination quality (g·kg^-1)
育苗后14 d 14 d after seedling	CK	9.61±3.16c	3.94±0.64c
	T1	39.89±4.23a	13.65±1.08a
	T2	18.70±7.18b	6.34±1.73b
	T3	8.95±1.83c	1.60±1.15d
	T4	8.20±1.90c	0.35±0.06d
育苗后21 d 21 d after seedling	CK	25.20±10.81b	33.12±5.95b
	T1	49.01±5.49a	63.82±5.92a
	T2	34.97±12.73ab	39.38±7.50b
	T3	32.34±2.36ab	34.39±5.37b
	T4	28.94±7.31b	20.73±3.44c
育苗后28 d 28 d after seedling	CK	58.15±5.29b	76.52±15.09c
	T1	84.38±5.24a	120.40±7.12a
	T2	85.54±10.22a	94.99±3.41b
	T3	50.51±17.15b	41.25±6.89d
	T4	15.20±4.33c	16.17±3.75e

Exogenous Brassinolide Alleviates the Inhibitory Effect of SPVD on Sweet Potato Storage Roots Germination

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