Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (24): 5240-5250.doi: 10.3864/j.issn.0578-1752.2021.24.007

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGRICULTURAL ECONOMY & MANAGEMENT • Previous Articles     Next Articles

Application Effect of Fungi Promoting Secondary Fermentation in Composting

WEI QiHang1,2(),FENG Yao2,MA QianQian2,LI YanLi2,LIU YuanWang2,LI ZhaoJun2,*(),REN YanFang1,*()   

  1. 1School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, Jiangsu
    2Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Beijing 100081
  • Received:2020-12-11 Accepted:2021-03-22 Online:2021-12-16 Published:2021-12-28
  • Contact: ZhaoJun LI,YanFang REN E-mail:15996166142@163.com;lizhaojun@caas.cn;yanfangren@126.com

Abstract:

【Objective】Secondary fermentation is indispensable to improve the functional value of chicken manure or other breeding wastes. This study applied the screened fungi in the secondary fermentation stage of chicken manure composting to explore the effect of the fungi on promoting the secondary fermentation, and the fungi were identified, so as to provide the theoretical basis and support for the high value and resource utilization of chicken manure. 【Method】The products of chicken manure after primary fermentation were used as raw materials, and two strains of fungi ( FCM1 and FCM3 ) were previously screened in the laboratory and were added before secondary fermentation. Three treatments were set up, including raw materials mixed with 1% (V:W,L·kg-1) sterile liquid medium (CK), raw materials mixed with 1% liquid medium containing FCM1 (F1), and raw materials mixed with 1% liquid medium containing FCM3 (F3), and some indexes were detected, including temperature, pH, electroconductibility (EC), ammonium nitrogen, nitrate nitrogen, GI and humic acid in different secondary fermentation stages. 【Result】Compared with CK, the addition of fungi could promote the temperature rise and humification process during the secondary fermentation stage. Compared with the CK, F3 treatment reached the mesophilic period 8 days earlier, but F1 treatment delayed the second fermentation of chicken manure composting. As for F3 treatment, the thermophilic stage (>50℃) lasted 13 days, GI reached 80% at 25 days earlier than that under CK, and the humification degree was relatively higher (PHA=74.58%, PHA: the ratio of humin and total humus). The correlation analysis showed that GI had significant positive correlation with pH and PHA, and negative correlation with EC. In addition, FCM3 was identified as Alternaria sp. by morphology and ITS rDNA sequencing. 【Conclusion】A strain of fungus FCM3 could promote the secondary fermentation of chicken manure composting, and improve the humification of composting materials, which was of great significance to promote the high value utilization of chicken manure.

Key words: secondary fermentation, fungi, composting maturity, high value utilization, Alternaria

Table 1

Physical and chemical properties of compost raw materials"

指标
Index
pH EC
(mS·cm-1)
含水率
Moisture content (%)
有机碳
Organic carbon (g·kg-1)
全氮
Total nitrogen (g·kg-1)
种子发芽指数
Germination index (%)
原料 Raw materials 6.29±0.09 5.94±0.11 49.47±1.90 324.70±9.45 27.39±1.35 19.35±5.64

Fig. 1

Changes of temperature during secondary fermentation"

Fig. 2

Changes of pH and EC during secondary fermentation"

Fig. 3

Changes of NH4+-N and NO3--N during secondary fermentation"

Fig. 4

Changes of GI during secondary fermentation"

Fig. 5

Changes of total humus, humic acid, fulvic acid and PHA during secondary fermentation"

Fig. 6

Changes of F3 treatment materials during secondary fermentation"

Table 2

Pearson correlation analysis of indexes in F3 treatment"

指标 Index pH EC NH4+-N NO3--N PHA GI
pH 1
EC -0.837** 1
NH4+-N 0.945** -0.725* 1
NO3--N -0.496 0.524 -0.571 1
PHA 0.920** -0.937** 0.791* -0.551 1
GI 0.982** -0.865** 0.908** -0.498 0.928** 1

Fig. 7

Colony morphology of FCM3"

Fig. 8

Phylogenetic tree of FCM3"

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