Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (20): 3595-3604.doi: 10.3864/j.issn.0578-1752.2019.20.010

• PLANT PROTECTION • Previous Articles     Next Articles

Development of 30% Clothianidin·Pyraclostrobin·Difenoconazole Flowable Concentrate for Seed-Coating and Its Application Effect in Peanut Field

HaiChao CAO1,QingShun LIU2,HaiXiu BAI3,Jun HAN4,ShiLing YANG5,Ming XUE1,Feng LIU1()   

  1. 1 College of Plant Protection, Shandong Agricultural University/Shandong Key Laboratory of Pesticide Toxicology & Application Technique, Taian 271018, Shandong
    2 Qingdao Taisheng Agriculture Technology Co., Ltd, Qingdao 266101, Shandong
    3 Shandong Medicine Technician College, Taian 271018, Shandong
    4 Shandong SINO-AGRI Union Biology Science Co., Ltd, Ji’nan 255001
    5 Xintai Agricultural and Rural Bureau, Xintai 271200, Shandong
  • Received:2019-05-15 Accepted:2019-06-27 Online:2019-10-16 Published:2019-10-28
  • Contact: Feng LIU E-mail:fliu@sdau.edu.cn

Abstract:

【Objective】 The objective of this study is to evaluate the safety of 30% clothianidin·pyraclostrobin·difenoconazole flowable concentrate for seed-coating (FS) on the growth of peanut (Arachis hypogaea) and its application potential in peanut soil-borne diseases and underground pests control.【Method】 30% clothianidin·pyraclostrobin·difenoconazole flowable concentrate for seed-coating was prepared and its pH, viscosity, suspension rate, cold and heat storage stability and the change of particle size distribution of agent after cold and heat storage tests were measured. 30% clothianidin·pyraclostrobin·difenoconazole FS was used to treat peanut seeds in the greenhouse with three doses of 200, 400 and 800 g a.i./100 kg seeds. At 14 days after sowing, the seedling emergence time and plant height, root length, fresh weight of stalk, leaf and root, and plant dry weight were measured to evaluate the safety of seed-coating for peanut seedling emergence and growth. In the field, three doses of 100, 200 and 400 g a.i./100 kg seeds were set for peanut seed-coating, and three efficacy trials in different places were conducted in two years. The 25% thiamethoxam·fludioxonil·metalaxyl-M FS (Maishuping) was selected as control agent.【Result】 The quality of 30% clothianidin·pyraclostrobin·difenoconazole flowable concentrate for seed-coating conformed to the standard GB/T17768—1999 of flowable concentrate for seed-coating of pesticide product. In the greenhouse pot experiment, the agent was safe for peanut seedling emergence and growth with 200, 400 and 800 g a.i./100 kg seeds. Field trials were conducted in 2016 with three doses of 100, 200 and 400 g a.i./100 kg seeds, the control efficacy on peanut crown rot was 95.16%, 97.98% and 98.79%, on peanut root rot was 90.97%, 92.26% and 92.90%, and against Aphis medicaginis was 79.74%, 92.48% and 94.13%, respectively, and the control efficacy on pests increased with the increase of dose. Compared with the blank control treatment, the peanut emergence rate increased by 10.25%-13.21%, and the peanut yield increased by 683.75-1 234.12 kg·hm -2. Therefore, 200 g a.i./100 kg seeds was selected as the field recommended dose in 2017. In the two field trials in 2017, which conducted in Xintai and Taian of Shandong Province, the control efficacy on peanut crown rot was 81.12% and 95.83%, on peanut root rot was 71.74% and 92.93%, and against cutworm in Xintai and on peanut stem rot in Taian was 93.33% and 87.29%, respectively. Compared with the blank control treatment, the peanut emergence rate increased by 4.23% and 10.75%, and the yield increased by 1 146.45 and 1 715.70 kg·hm -2, in Xintai and Taian trials, respectively.【Conclusion】 30% clothianidin·pyraclostrobin·difenoconazole flowable concentrate for seed-coating has good control efficacy on peanut crown rot, root rot, stem rot, A. medicaginis and cutworms, it is safe for the peanut growth and emergence and can improve the peanut yield. Therefore, the seed-coating has a good promotion prospect.

Key words: peanut (Arachis hypogaea), seed-coating agent, pesticide and fungicide mixture, soilborne disease, underground pest, formulation processing

Table 1

Physical and chemical properties of SBB formulation"

热贮析水率
Syneresis rate after
heat storage (%)
粒度分布(D50,μm) 黏度
Viscosity
(mPa.s)
悬浮率
Suspension rate (%)
pH
贮前
Before storage
热贮后
After heat storage
冷贮后
After cold storage
3.03±0.71 2.21±0.06 2.53±0.22 2.23±0.05 372.63±2.89 98.37±0.38 6.90±0.12

"

处理Treatment 用量Dosage (g a.i./100 kg seeds) 株高Plant height (cm) 根长Root length (cm)
SBB 200 15.94±0.40a 13.56±1.16a
400 15.76±0.28a 14.34±0.98a
800 15.58±0.18a 13.10±1.12a
清水Water 15.84±0.24a 14.19±1.16a

Table 3

The effects of seed-coating with SBB on fresh weight of peanut seeding stalk and leaf, root fresh weight and plant dry weight"

处理
Treatment
用量
Dosage (g a.i./100 kg seed)
茎叶鲜重
Stalk and leaf fresh weight (g)
根鲜重
Root fresh weight (g)
植株干重
Plant dry weight (g)
SBB 200 7.38±0.25a 1.23±0.06a 0.87±0.04a
400 7.86±0.31a 1.31±0.11a 0.80±0.07a
800 7.54±0.27a 1.46±0.07a 0.78±0.03a
清水Water 7.12±0.44a 1.58±0.09a 0.75±0.05a

Table 4

The control efficacy of SBB coating seeds against peanut crown rot, root rot and A. medicaginis in the fields of Liangzhuang, Taian (2016)"

药剂
Pesticide
用量
Dosage
(g a.i./
100 kg seeds)
冠腐病 Crown rot 根腐病 Root rot 花生蚜 A. medicaginis
病株数
Infected plants (plants per plot)
防治效果
Control efficacy
(%)
病株数Infected plants (plants per plot) 防治效果
Control efficacy
(%)
虫口数
Pest number (individuals per clump)
防治效果
Control efficacy
(%)
SBB 100 4.00±0.58d 95.16±0.70a 4.67±0.88bc 90.97±1.71a 50.71±2.65b 79.74±1.06b
200 1.70±0.88d 97.98±1.07a 4.00±2.31c 92.26±4.47a 18.86±3.22c 92.48±1.28a
400 1.00±0.57d 98.79±0.70a 3.67±1.45c 92.90±2.81a 14.71±4.17c 94.13±1.67a
迈舒平 Maishuping 100 19.67±2.03b 76.21±5.94b 10.67±3.18b 79.31±6.15b 75.29±24.75b 69.90±9.87b
200 12.00±1.15c 85.48±1.40b 3.00±0.58c 94.19±1.12a 16.86±3.44c 93.28±1.37a
清水Water 82.67±4.26a 51.67±2.60a 250.71±22.15a

Table 5

The control efficacy of SBB coating seeds against peanut crown rot, root rot and cutworm in the fields of Liudu, Xintai (2017)"

药剂
Pesticide
用量
Dosage
(g a.i./
100 kg seeds)
冠腐病 Crown rot 根腐病 Root rot 地老虎 Cutworm
病株数
Infected plants (plants per plot)
防治效果
Control efficacy
(%)
病株数
Infected plants (plants per plot)
防治效果
Control efficacy
(%)
被害株数
Destroyed plants (plants per plot)
防治效果
Control efficacy
(%)
SBB 200 25.00±7.64b 81.12±5.08a 26.67±7.26b 71.74±5.81a 1.67±1.67c 93.33±6.67a
迈舒平 Maishuping 200 35.00±13.23b 75.01±7.23a 35.00±7.64b 63.25±5.27a 13.33±4.41b 57.67±19.20b
清水Water 133.33±14.24a 93.33±11.67a 35.00±7.64a

Table 6

The control efficacy of SBB coating seeds against peanut crown rot, root rot and stem rot in the fields of Qiujiadian, Taian (2017)"

药剂
Pesticide
用量
Dosage
(g a.i./100 kg seeds)
冠腐病 Crown rot 根腐病 Root rot 茎腐病 Stem rot
病株数
Infected plants (plants per plot)
防治效果
Control efficacy
(%)
病株数Infected plants (plants per plot) 防治效果
Control efficacy
(%)
病株数Infected plants (plants per plot) 防治效果
Control efficacy
(%)
SBB 200 3.67±0.29b 95.83±0.84a 2.67±1.45b 92.93±4.01a 15.67±2.91c 87.29±1.94a
迈舒平 Maishuping 200 6.00±0.58b 93.15±0.16a 3.67±0.33b 91.34±0.71a 38.33±0.67b 69.23±2.60b
清水Water 88.00±10.39a 40.67±2.60a 132.00±2.89a

Fig. 1

The scene of SBB coating seeds in controlling peanut stem rot, the diseased plant and pathogen of peanut stem rot in Qiujiadian, Taian"

Fig. 2

Effect of SBB coating seeds on peanut emergence rate and yield, which counted by peanut pod fresh weight"

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