Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (18): 3959-3969.doi: 10.3864/j.issn.0578-1752.2021.18.014

• HORTICULTURE • Previous Articles     Next Articles

Effects of Tea-Litsea Cubeba Intrercropping on Soil Microbial Community Structure in Tea Plantation

HAO HaiPing1,2(),BAI HongTong1,XIA Fei1,HAO YuanPeng1,LI Hui1,CUI HongXia1,XIE XiaoMing3,SHI Lei1()   

  1. 1Key Laboratory of Plant Resources/Institute of Botany, Chinese Academy of Sciences, Beijing 100093
    2Department of Life Sciences, Langfang Normal University, Langfang 065000, Hebei
    3Qianxin Town Experimental Base, Institute of Botany, Chinese Academy of Sciences, Jiangxi I Chan Eco-Agriculture Co., Ltd, Longnan 341708, Jiangxi
  • Received:2020-11-23 Accepted:2021-01-07 Online:2021-09-16 Published:2021-09-26
  • Contact: Lei SHI E-mail:haohaiping2014@126.com;shilei@ibcas.ac.cn

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Abstract:

【Objective】 The aim of this study was to analyze the changes of soil microbial community structure and its driving factors, so as to provide data support for soil ecological effect evaluation of Tea-Litsea cubeba intercropping. 【Method】GS-MS, ICP-MS, 16S and ITS sequence analysis were used to determine the soil extract composition, soil mineral element content, soil bacteria and soil fungi community structure.【Result】 The soil microbial community structure was significantly affected by the model of Tea-Litsea cubeba intercropping. The abundance of soil functional bacteria related to N, P and Mn transformation increased significantly, while the abundance of plant pathogenic bacteria and fungi was significantly decreased. In the root distribution layer, the abundance of Burkholderia paraburkholderia was 86 times compared to control, and the abundance of Fusarium graminearum was 73.13% lower than that of the control. Tea-Litsea cubeba intercropping increased the P content of tea plantation soil. In the root distribution layer, P content increased by 76.42%. The soil of Tea-Litsea cubeba intercropping contained some antibacterial substance, including 12.80% camphor, 6.72% Alpha-terpineol, and 12.65% citronellol. Redundancy analysis (RDA) showed that the soil extraction and P in the intercropping area were the main environmental factors affecting the community structure of soil microbes.【Conclusion】 Tea-Litsea cubeba intercropping changed the abundance and community structure of soil microbes, and the camphor, Alpha-terpineol, citronellol and P were the main ecological factors that affected the soil microbial community. The selection of intercropping plants in tea plantations and other farmland systems should pay attention to the secondary metabolites that enter the soil, especially those with killing or antibacterial functional components.

Key words: Litsea cubeba, tea, intercropping, soil microbes, soil properties

Table 1

List of controls and experimental treatments"

处理编号
Treatment number		 土层 Soil layer
表土层
Top soil layer
(0—10 cm)		 根系分布层
Root distribution layer
(10—30 cm)
1 CK.1 CK.2
2 Lit.1 Lit.2

Fig. 1

Rarefaction curves of all samples were generated for microbial OTUS which contained unique sequences and were defined at 97% sequence similarities A: Rarefaction curves of soil bacteria; B: Rarefaction curves of soil fungi"

Fig. 2

Estimated number of observed OTU counts, Shannon index, Chao 1 index of soil microbiome of tea plantation across all the intercropping with Litsea cubeba plots A, B, C: The index of bacterial; D, E, F: The index of Fungi. n=3. * indicate significant difference (P<0.05). The same as below"

Fig. 3

The NMDS analysis of soil microbials A: Bacterial; B: Fungi"

Fig. 4

The relative abundance of phyla of soil microbiome in the tea-Litsea cubeba intrercropping plots A: Bacterial; B: Fungi"

Table 2

List of microbe’s functions"

微生物
Microbes		 潜在功能
Potential functions		 丰度 OTU number
CK.1 Lit.1 CK.2 Lit.2
细菌
Bacteria		 黄单胞杆菌Norank xanthomonadales 植物病原菌 Plant pathogens 218 0 493 0
伯克氏菌
Burkholderia paraburkholderia		 溶解磷;生物降解
Decomposition and release P; Biodegradation		 1 63 0 86
黄色菌 Norank xanthobacteraceae 五氯硝基苯降解 Pentachloronitrobenzene degradation 2 1523 8 851
慢生根瘤菌 Bradyrhizobium 固氮 Nitrogen fixation 269 662 315 385
土微菌 Pedomicrobium 锰氧化 Manganese oxidantion 0 241 1 214
真杆菌 Norank acidobacteria 还原硝酸盐 Nitrate reduction 1 484 3 604
栖热菌属 Acidothermus 还原硝酸盐 Nitrate reduction 18 84 121 49
浮霉状菌属
Planctomyces		 缺氧条件下氧化铵生成氮气
Amine generates nitrogen in anoxic environment		 0 78 2 63
真菌
Fungi		 镰刀霉 Fusarium graminearum 致病菌 Pathogenic bacteria 4729 695 3122 839
毛壳菌 Chaetomium 纤维素分解 Cellulose breakdown 1 624 4 255
拟康宁木霉 Trichoderma koningiopsis 生物防治菌 Biocontrol bacteria 41 1589 29 6942
山野壳菌 Paraphaeosphaeria 病原真菌 Pathogenic fungi 145 14 216 33

Table 3

Soil extract of Litsea cubeba intercropping treatment"

峰 Peak 时间 RT 面积 Area 峰面积比例 Peak area ratio (%) 名称 Name
1 4.42 108958 3.01 苯乙烯 Phenylethylene
2 6.01 135087 3.73 桉叶油醇 Cineole
3 7.17 463228 12.80 樟脑 (+)-2-Bornanone
4 7.64 243391 6.72 Alpha-松油醇 Alpha-Terpineol
5 7.99 457853 12.65 香茅醇 Citronellol
6 8.30 90066 2.49 乙酸冰片酯 Bornyl acetate
7 8.74 103561 2.86 乙酸香茅酯 Citronellol acetate
8 9.00 61857 1.71 丙酸 Propanoic acid
9 9.35 293552 8.11 羟基香茅醛 4-Octene-2,7-diol
10 11.08 48177 1.33 2-甲基十七烷 Dibuyl phthalate
11 12.10 132366 3.66 邻苯二甲酸二异丁酯 1,2-Benzenedicarboxylic acid
12 12.68 280323 7.74 邻苯二甲酸二正丁酯 Dibuyl phthalate
13 14.37 167173 4.62 棕榈酰胺 Hexadecanamide
14 16.13 1034060 28.57 油酸酰胺 Oleamide

Fig. 5

Effects of of Litsea cubeba intercroping on soil mineral nutriments"

Fig. 6

Redundancy analysis of soil microbial and environmental factors In the plot, red hollow arrows represent environmental factors; solid arrows stand for microbial community structure information; cosine of the angle between the extension lines of environmental factor and microbial species equals to the correlation coefficient between the two in numerical value"

Table 4

Redundancy analysis of soil microorganisms and environment factors"

环境因子
Name		 关联度
Explain		 Pseudo -F值
Pseudo-F		 P值
P
Ci
桉叶油醇 Cineole		 48.4 3.7 0.03*
Alp
Alpha-松油醇 Alpha-Terpineol		 48.1 3.7 0.01*
Ph
苯乙烯 Phenylethylene		 48.0 3.7 0.018*
Bo
樟脑 (+)-2-Bornanone		 48.0 3.7 0.034*
Cit
香茅醇 Citronellol		 47.8 3.7 0.028*
P
磷 Phosphorus		 47.8 3.7 0.04*
Fe
铁 Iron		 46.7 3.5 0.068
Zn
锌 Zinc		 46.1 3.4 0.06
Mn
锰 Manganese		 44.4 3.2 0.112
Mg
镁 Magnesium		 43.5 3.1 0.06
Cu
铜 Copper		 36.1 2.3 0.114
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