Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (6): 1269-1277.doi: 10.3864/j.issn.0578-1752.2020.06.018

• ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

The Relationship Between Nutrients and Biological Yield of Different Varieties of Alfalfa

ZeMin LI,Chen ZHANG,ChongYu ZHANG(),GuiGuo ZHANG()   

  1. College of Animal Science and Technology, Shandong Agricultural University/Key Laboratory of Animal Biotechnology and Disease Control & Prevention of Shandong Province, Tai’an 271018, Shandong
  • Received:2019-02-25 Accepted:2019-10-28 Online:2020-03-16 Published:2020-04-09
  • Contact: ChongYu ZHANG,GuiGuo ZHANG E-mail:17225122@163.com;zhanggg@sdau.edu.cn

Abstract:

【Objective】To determine the content and the variation degree of nutritive and the relationship between the content of various fiber components in different alfalfa varieties. Compare the biological yields of different alfalfa varieties.【Method】Two experiments were conducted. In Experiment 1, selected 16 alfalfa varieties including WL343HQ, WL353HQ, WL354HQ, WL363HQ, WL366HQ, Algonuin, Golden empress, Zhongmu No.3, Zhongmu No.6, Luduo, Luhuang No. 1, Wudi, Lufeng No. 1, Baoding, Zhongyuan804, Aohan and planted on October 2, 2016. Each variety was set up twice and randomly arranged plots. The planting area of each alfalfa variety was 18 m 2 (3 m×6 m) , row spacing was 30 cm, and 10 rows were sown. Harvested at the beginning of flowering on May 23, 2018 and determined the biological yield. In experiment 2,prepared 32 samples and determined the content of nutrients such as crude ash (ASH), crude protein (CP), crude fat (EE), crude fiber (CF),neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin (ADL) in the dry matter (DM). 【Result】 The results showed that: (1) In alfalfa DM of different varieties, the contents of ASH, NDF, ADF, HCEL, CEL and ADL were significantly different (P<0.01), but the difference of EE content was not significantly (P=0.527). The ASH content of WL363HQ alfalfa was the highest, which was 8.53%. The highest CP content of golden empress alfalfa was 27.19%. The content of EE and CF in baoding 804 alfalfa was the highest, which were 3.01% and 36.69%, respectively. The content of NDF, ADF, CEL and ADL in zhongyuan 804 alfalfa was the highest, which were 57.02%, 42.73, 34.43% and 7.92% respectively. The average values of ASH, CP, EE, CF, NDF, ADF, ADL, CEL and HCEL in alfalfa DM of different varieties were 7.83%, 21.58%, 2.51%, 33.25%, 50.12%, 36.76%, 6.98%, 29.43% and 13.36%, respectively; The coefficient of variation (CV) is between 5.16% and 12.06%. (2) There was a strong correlation between ADF and CEL, ADL content in alfalfa, and the correlation coefficients were 0.9756, 0.9955 and 0.9455. The correlation coefficients between CF and ADF, CEL, ADL content were 0.9114, 0.8849 and 0.9375, respectively. The correlation coefficient between CF (including AIA) and CF was 0.9997. The correlation coefficients between NDF and CEL, ADL, HCEL content were 0.9671, 0.9396 and 0.8736, respectively. The correlation coefficient between ADL (including AIA) and ADL was 0.9989. The correlation coefficients between RFA and ADF, CEL were 0.9826 and 0.9700. (3) There was a strong correlation between relative feeding value (RFV) and NDF content (r=0.9938), which can be used for the estimation of alfalfa RFV: RFV=287.0677-3.4709 NDF (%) n=16 P<0.01. (4) The yields of different alfalfa varieties were different. Baoding alfalfa has the highest yield of crude fat, carbohydrate, hemiculose and cellulose, which were respectively 393.59, 9 308.22, 1 976.32 and 4 208.05 kg·hm -2; The yield of golden empress's crude protein was the highest, which was 2 697.23 kg·hm -2, and the yield of Baoding's digestible energy (DE) was the highest, which was 126 814.45 MJ·hm -2. 【Conclusion】There was a strong correlation between the content of different alfalfa fiber components, and the RFV value of alfalfa was strongly correlated with the NDF component; The yields of different alfalfa varieties are different.

Key words: alfalfa, nutrients, biological yield

Table 1

Contents of various nutrients and DE、NND values in alfalfa DM of different varieties"

品种名称
Name
灰分
ASH
(%)
粗蛋白质
CP
(%)
粗脂肪
EE
(%)
粗纤维
CF
(%)
中性洗涤纤维NDF(%) 酸性洗涤纤维ADF(%) 半纤维素HCEL
(%)
纤维素CEL
(%)
木质素ADL
(%)
消化能
DE
(MJ·kg-1)
奶牛能量单位
NND
WL343HQ 7.94 23.03 2.55 29.68 47.16 33.35 13.80 26.72 6.29 10.49 1.71
WL353HQ 7.44 22.82 2.36 32.52 47.89 36.07 11.82 28.96 6.78 10.42 1.70
WL354HQ 8.23 24.09 2.73 27.39 41.65 30.25 11.41 24.62 5.30 10.95 1.79
WL363HQ 8.53 23.70 2.40 28.97 42.96 31.98 10.98 25.89 5.76 10.73 1.76
WL366HQ 7.97 20.97 2.78 32.23 46.66 34.12 12.54 26.81 6.88 10.41 1.70
阿尔冈金Algonuin 8.02 20.32 2.53 35.53 50.04 37.95 12.08 30.23 7.35 10.07 1.64
金皇后 Golden empress 7.87 27.19 2.44 31.70 51.14 36.45 14.68 28.93 7.17 10.26 1.67
中苜3号 Zhongmu No.3 7.52 18.72 2.72 35.15 53.71 39.69 14.02 32.17 7.15 9.86 1.60
中苜6号 Zhongmu No.6 7.13 18.86 2.20 36.07 50.07 36.70 13.37 29.04 7.29 10.16 1.66
鲁多Luduo 7.73 22.43 2.82 30.50 46.57 33.77 12.80 27.12 6.33 10.55 1.72
鲁黄1号 Luhuang No 1 7.24 25.08 2.44 33.39 53.48 38.05 15.43 30.50 7.25 10.12 1.65
无棣Wudi 7.85 19.99 2.02 35.38 52.52 39.14 13.38 31.19 7.57 9.87 1.60
鲁丰1号 Lufeng No 1 8.28 18.90 2.71 35.13 50.93 37.31 13.62 29.91 7.09 9.97 1.62
保定Baoding 7.43 18.37 3.01 36.69 55.59 40.47 15.11 32.18 7.88 9.70 1.57
中原804 Zhongyuan804 7.77 21.52 2.15 36.10 57.02 42.73 14.30 34.43 7.92 9.57 1.55
敖汉Aohan 8.36 19.28 2.31 35.58 54.56 40.18 14.38 32.23 7.63 9.62 1.56
SEM 0.06 0.35 0.06 0.43 0.47 0.38 0.16 0.38 0.10 0.10 0.02
P P<0.001 P<0.001 0.527 P<0.001 P<0.001 P<0.001 P<0.001 P<0.001 P<0.001 P<0.001 P<0.001

Table 2

Average value and coefficient of variation of nutrient content in alfalfa DM of different varieties (%)"

平均值
Average value
标准差
Standard deviation
变异系数
Coefficient of variation
ASH 7.83 0.40 5.16
CP 21.58 2.60 12.06
EE 2.51 0.27 10.78
CF 33.25 2.91 8.76
NDF 50.12 4.40 8.78
ADF 36.76 3.39 9.21
ADL 6.98 0.74 10.54
CEL 29.43 2.69 9.15
HCEL 13.36 1.32 9.90
RFV 113.10 15.36 13.58

Table 3

Parameters and correlation coefficient r in y=a+bx model (n=16)"

y a b x r P<
CP 42.3190 -0.4352 NDF 0.8996 0.01
CP 40.9850 -0.5548 ADF 0.8842 0.01
ADF -0.8917 0.7513 NDF 0.9756 0.01
ADF -0.0745 1.2516 CEL 0.9955 0.01
ADF 6.3668 4.3563 ADL 0.9455 0.01
CF 4.4468 0.7835 ADF 0.9114 0.01
CF 5.0984 0.9565 CEL 0.8849 0.01
CF 7.3407 3.7134 ADL 0.9375 0.01
CF 101.4800 -1.9437 NFE 0.8899 0.01
CF+AIA 0.4001 1.0195 CF 0.9997 0.01
NDF 3.6470 1.5790 CEL 0.9671 0.01
NDF 10.8958 5.6218 ADL 0.9396 0.01
NDF 13.3550 2.7524 HCEL 0.8736 0.01
NDF 80.0210 -1.5833 NDSC 0.9678 0.01
ADL -0.3358 0.2485 CEL 0.9105 0.01
ADL+AIA 0.2188 1.0192 ADL 0.9989 0.01
RFV 287.0677 -3.4709 NDF 0.9938 0.01
RFV 276.9435 -4.4568 ADF 0.9826 0.01
RFV 275.9090 -5.5315 CEL 0.9700 0.01

Table 4

Biological yield of various nutrients and DE、NND values in alfalfa of different varieties"

品种名称
Name
粗蛋白质
CP
(kg·hm-2)
粗脂肪
EE
(kg·hm-2)
碳水化合物
CHO
(kg·hm-2)
半纤维素
HCEL
(kg·hm-2)
纤维素
CEL
(kg·hm-2)
消化能
DE
(MJ·hm-2)
奶牛能量单位NND
(hm2)
WL343HQ 2253.71 250.03 6506.62 1351.02 2615.69 102681.58 17998.47
WL353HQ 2455.22 254.23 7249.90 1271.74 3116.11 112153.82 19658.98
WL354HQ 2322.58 263.25 6262.22 1099.66 2374.13 105541.02 18499.74
WL363HQ 2490.31 252.43 6869.20 1153.67 2720.30 112789.02 19770.33
WL366HQ 2451.23 324.87 7980.74 1465.29 3134.38 121632.53 21320.62
阿尔冈金Algonuin 2437.47 303.09 8290.90 1449.29 3626.14 120732.75 21162.89
金皇后Golden empress 2697.23 242.20 6198.24 1456.48 2869.70 101782.05 17840.78
中苜3号Zhongmu No.3 1991.85 289.57 7556.72 1491.12 3422.49 104929.85 18392.60
中苜6号Zhongmu No.6 1602.59 187.13 6099.31 1135.65 2467.11 86333.43 15132.60
鲁多Luduo 1850.75 232.54 5530.30 1056.07 2237.58 87010.45 15251.28
鲁黄1号Luhuang No.1 2444.71 237.69 6360.28 1504.39 2973.62 98659.81 17293.44
无棣Wudi 1884.46 190.32 6612.01 1261.65 2939.84 93034.50 16307.31
鲁丰1号Lufeng No.1 2351.96 337.10 8725.49 1695.36 3721.97 124131.94 21758.78
保定Baoding 2402.24 393.59 9308.22 1976.32 4208.05 126814.45 22229.03
中原804 Zhongyuan804 2661.73 266.23 8476.87 1768.02 4257.91 118381.97 20750.79
敖汉Aohan 2533.91 303.58 9182.16 1889.75 4235.35 126764.82 22220.33
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