Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (17): 3576-3586.doi: 10.3864/j.issn.0578-1752.2020.17.014

• HORTICULTURE • Previous Articles     Next Articles

Optimum Content of Mineral Elements in the Leaves of Duweiwendan Pomelo (Citrus grandis (L.) Osbeck. cv. Duweiwendan)

WANG XianDa(),FAN GuoCheng,LI Jian()   

  1. Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013
  • Received:2020-01-23 Accepted:2020-05-20 Online:2020-09-01 Published:2020-09-11
  • Contact: Jian LI E-mail:564944260@qq.com;Fujianlijian@126.com

Abstract:

【Objective】The aim of this study was to research and construct a diagnosis system for mineral nutrients in the leaves of Duweiwendan Pomelo (Citrus grandis (L.) Osbeck. cv. Duweiwendan), so as to deduce a shared optimization method for the mineral nutrition balance diagnosis and recommendation integrated system (BDRIS).【Method】From 2016 to 2018, 20 representative orchards with different managers were selected in the production area of Xianyou county in Fujian Province. 200 individual leaf samples and 8 660 single fruit samples were collected, and 48 pairs of heteromorphic fruits (H-FSI normal fruit and L-FSI cracking fruit) with the same axillary growth were collected, and the contents of N, P, K, CA, Mg, Cu, Zn, Fe, Mn, B, Mo and S elements in leaves and pericarps were measured. The internal and external quality of fruits and the width of top crack were also measured. Based on the difference between the total soluble solids (TSS) and the crack average index (FCAI) of the fruit, a BDRIS diagnosis system for major mineral elements and a diagnostic criterion for critical values of trace elements were constructed.【Result】According to the difference analysis of TSS, rich N, P, K and lack of Mo significantly reduced TSS. According to the FCAI12 difference analysis, except that the suspected B toxicity excess (>177 mg·kg-1) significantly increased the fruit cracking, no significant causal relationship was found between other mineral elements and the fruit cracking, but no evidence of fruit cracking caused by B toxin was found; in addition, no significant difference was found in the paired comparative analysis of the elements in pericarp of twin shaped fruit. The distribution of N, P, K, CA, and Mg elements in the selected samples met the requirements of BDRIS modeling, P (normal) ≥0.12. According to BDRIS construction, it was found that the tree age was significantly negatively correlated with BDRIS index, and significantly positively correlated with TSS, that is to say, with the growth of tree age, the mineral nutrition of tree body tended to the origin of nutrition balance, and the fruit quality was also better, which gave a new explanation of the common sense of fruit tree cultivation that “the older the tree is, the sweeter the fruit will be”. BDRIS had no significant correlation with FCAI12; by correlation method, only when K was in morbid excess could thicken the peel, so as to reduce the cracking, which had no practical significance in correcting fruit cracking in production. It was suggested that the diagnostic standard of critical value of mineral elements in leaves should be “elements (over)”: N (<2.29%; 2.41%- 2.87%; >2.99%), P (<0.09%; 0.10%-0.14%; >0.15%), K(<1.17%; 1.39%-2.24%; >2.46%), Ca (<1.74%; 2.26%-4.21%; >4.72%), Mg (<0.20%; 0.24%-0.41%; >0.46%), Cu (<4 mg·kg -1; 6-25 mg·kg-1; >30 mg·kg-1), Zn (24-40 mg·kg-1), Fe (60-140 mg·kg-1), Mn (25-140 mg·kg-1), B (<15 mg·kg-1; 30-65 mg·kg-1; >150 mg·kg-1), Mo (<0.05 mg·kg-1; 0.1-1.0 mg·kg-1), and S (0.2%-0.4%). The shared optimization algorithm of BDRIS diagnosis parameters Mean, Std and r was provided to effectively expand the application scope of BDRIS.【Conclusion】In this study, the BDRIS diagnosis system for major mineral elements and the diagnostic criterion for critical values of trace elements were established based on the high-yield and high-quality population. In addition, there was no significant correlation between fruit cracking and the plant mineral nutrition.

Key words: Duweiwendan pomelo, mineral nutrients, appropriate standard, BDRIS

Table 1

Variance analysis of leaf mineral nutrition based on TSS difference of fruit"

元素
Element
平均值Mean
TSS≥TSSMean
n1=61
平均值Mean
TSS<TSSMean
n2=59
D 方差分析
ANOVA
协方差分析
Co-ANOVA
协变量
Co-factor
F P F P F P
可溶性固形物 TSS (%) 10.66 9.27 1.391 118.45 <.0001 - - - -
单果质量 SFW (g) 686.3 741.4 -55.10 - - 10.95 0.0013 67.98 <.0001
果形指数 FSI 0.8698 0.8733 -0.0035 0.22 0.6419 - - 0.56 0.4552
N (%) 2.861 2.955 -0.094 6.53 0.0120 - - 0.08 0.7831
P (%) 0.1325 0.1382 -0.006 - - 3.46 0.0655 22.27 <.0001
K (%) 2.295 2.444 -0.149 - - 6.64 0.0113 10.65 0.0015
Ca (%) 2.846 2.775 0.071 - - 0.41 0.5235 7.50 0.0072
Mg (%) 0.2683 0.2776 -0.009 0.93 0.3370 - - 0.00 0.9831
Cu (mg·kg-1) 15.66 15.94 -0.278 - - 0.76 0.3859 56.32 <.0001
Zn (mg·kg-1) 21.95 23.78 -1.837 - - 2.88 0.0928 12.50 0.0006
Fe (mg·kg-1) 76.26 72.81 3.450 - - 2.10 0.1501 5.80 0.0177
Mn (mg·kg-1) 49.30 57.82 -8.514 2.06 0.1538 - - 1.94 0.1667
B (mg·kg-1) 131.2 132.9 -1.705 0.05 0.8178 - - 3.33 0.0708
Mo (mg·kg-1) 0.0477 0.0398 0.008 10.25 0.0018 - - 0.10 0.7488
S (%) 0.3102 0.3065 0.004 - - 0.63 0.4308 9.75 0.0023

Table 2

Variance analysis of leaf mineral nutrition based on difference of FCAI"

元素
Element
平均值Mean
FCAI≥FCAIMean
n1=120
平均值Mean
FCAI<FCAIMean
n2=80
D 方差分析
ANOVA
协方差分析
Co-ANOVA
协变量
Co-factor
F P F P F P
果实裂宽平均指数 FCAI (%) 30.36 11.07 19.30 312.09 <.0001 - - - -
N (%) 2.863 2.863 0.00 0.00 0.9856 - - 1.67 0.1974
P (%) 0.1388 0.1377 0.00 - - 0.47 0.4932 10.68 0.0013
K (%) 2.218 2.256 -0.04 - - 1.16 0.2830 19.45 <.0001
Ca (%) 3.272 3.175 0.10 - - 1.36 0.2445 14.52 0.0002
Mg (%) 0.3072 0.2902 0.02 2.91 0.0899 - - 1.86 0.1743
Cu (mg·kg-1) 15.08 16.91 -1.82 - - 2.84 0.0937 52.55 <.0001
Zn (mg·kg-1) 22.22 20.93 1.30 - - 2.45 0.1192 8.28 0.0045
Fe (mg·kg-1) 79.54 86.77 -7.23 2.87 0.0921 - - 0.01 0.9367
Mn (mg·kg-1) 54.64 51.81 2.84 0.52 0.4696 - - 2.66 0.1045
B (mg·kg-1) 136.7 126.9 9.75 - - 3.92 0.0490 3.51 0.0625
Mo (mg·kg-1) 0.1409 0.1562 -0.02 - - 0.41 0.5233 8.46 0.0041
S (%) 0.3088 0.3005 0.01 - - 1.88 0.1723 10.28 0.0016

Table 3

Paired comparative analysis of mineral elements in peel of twin heteromorphic fruits (n=48)"

元素
Element
平均值 Mean 平均差值 △Mean
H-FSI正常果 L-FSI裂果 △Mean 标准差Std 变异系数 CV t P
N (%) 0.9135 0.9104 0.0031 0.0915 2921 0.23 0.8175
P (%) 0.0744 0.0750 -0.0006 0.0087 -1434 -0.47 0.6385
K (%) 1.4504 1.4439 0.0065 0.0843 1292 0.52 0.6022
Ca (%) 0.4857 0.4739 0.0118 0.0627 531 1.28 0.2081
Mg (%) 0.1269 0.1268 0.0001 0.0088 6759 0.10 0.9205
Cu (mg·kg-1) 5.5696 5.3089 0.2607 1.1753 451 1.50 0.1395
Zn (mg·kg-1) 6.4717 6.4009 0.0709 0.8177 1154 0.59 0.5596
Fe (mg·kg-1) 22.276 22.192 0.0846 3.3786 3995 0.17 0.8660
Mn (mg·kg-1) 11.263 11.696 -0.433 1.700 -392 -1.73 0.0908
B (mg·kg-1) 24.567 25.032 -0.465 1.996 -429 -1.58 0.1207
Mo (mg·kg-1) 0.0797 0.0764 0.0033 0.015 455 1.49 0.1427
S (%) 0.0679 0.0707 -0.0028 0.0091 -325 -1.51 0.1456

Table 4

Diagnostic criteria of mineral nutrition and Diagnostic parameters of major element BDRIS in Duweiwendan pomelo leaves"

元素
Element
临界标准[16] Critical standard BDRIS诊断参数 Diagnostic parameter (n=102)
缺乏
Lack
适宜
Suitable
过量
Over
r N P K Ca Mg
N (%) 2.29 2.41 2.87 2.99 N 1.0000 0.0293 0.0917 -0.1204 -0.0331
P (%) 0.09 0.10 0.14 0.15 P 0.0293 1.0000 0.4483 -0.0635 -0.0547
K (%) 1.17 1.39 2.24 2.46 K 0.0917 0.4483 1.0000 -0.6005 -0.2465
Ca (%) 1.74 2.26 4.21 4.72 Ca -0.1204 -0.0635 -0.6005 1.0000 0.0223
Mg (%) 0.20 0.24 0.41 0.46 Mg -0.0331 -0.0547 -0.2465 0.0223 1.0000
相对标准
Relative standard
-1.96 -1.28 1.28 1.96 平均值Mean 2.651 0.1218 1.854 3.232 0.3259
Cu (mg·kg-1) 4 6 25 35 标准差Std 0.1782 0.0150 0.3297 0.7612 0.0665
Zn (mg·kg-1) - 24* 40 - 正态检验
P (Normal)
0.9088 0.7427 0.2841 0.1831 0.1275
Fe (mg·kg-1) 60* 140*
Mn (mg·kg-1) 25* 140*
B (mg·kg-1) 15 30 65 150
Mo (mg·kg-1) 0.05 0.1* 1.0* -
S (%) 0.2* 0.4*

Table 5

Partial correlation analysis of main mineral elements in leaves and their BDRIS with fruit quality"

R BDRIS SFW TSS FSI FCAI12 Tree-age RTTD
氮 N 0.534 0.115 -0.324 -0.055 0.106 -0.198 0.180
磷 P 0.347 0.249 -0.265 0.248 -0.165 -0.245 0.101
钾 K 0.424 0.324 -0.247 0.199 -0.220 -0.279 0.202
钙 Ca -0.108 -0.206 0.144 -0.349 0.384 0.137 0.004
镁 Mg 0.029 0.088 -0.052 -0.129 -0.016 -0.046 0.059
BDRIS - 0.239 -0.216 0.126 -0.075 -0.412 0.140
单果重 SFW 0.239 - -0.411 0.356 -0.136 -0.075 0.509
可溶性固形物 TSS -0.216 -0.411 - -0.134 0.081 0.221 -0.328
果形指数 FSI 0.126 0.356 -0.134 - -0.684 -0.376 0.178
裂宽指数 FCAI12 -0.075 -0.136 0.081 -0.684 - 0.309 -0.204
树龄 Tree-age -0.412 -0.075 0.221 -0.376 0.309 - -0.177
果皮厚 RTTD 0.140 0.509 -0.328 0.178 -0.204 -0.177 -
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