Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (11): 2077-2091.doi: 10.3864/j.issn.0578-1752.2022.11.001


Application of Yr52 Gene in Wheat Improvement for Stripe Rust Resistance

FANG TaoHong1(),ZHANG Min1,MA ChunHua1,ZHENG XiaoChen1,TAN WenJing1,TIAN Ran1,YAN Qiong1,ZHOU XinLi1(),LI Xin1,YANG SuiZhuang1,HUANG KeBing1,WANG JianFeng2,HAN DeJun3,WANG XiaoJie2,KANG ZhenSheng2()   

  1. 1Wheat Research Institute, School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan
    2College of Plant Protection, Northwest A&F University/State Key Laboratory of Crop Stress Biology in Arid Areas, Yangling 712100, Shaanxi
    3College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology in Arid Areas, Yangling 712100, Shaanxi
  • Received:2022-01-11 Accepted:2022-02-28 Online:2022-06-01 Published:2022-06-16
  • Contact: XinLi ZHOU,ZhenSheng KANG;;


【Objective】 The objective of this study is to evaluate the application value of the high temperature adult plant (HTAP) resistance gene Yr52 in wheat production for improving stripe rust resistance. And wheat lines with good agronomic characters and high disease resistance were developed and selected. It laid a foundation for making full use of the existing HTAP resistance resources and improving the yield related traits.【Method】 The stripe rust resistance gene Yr52 was introgressed to Lunxuan 987 (LX987), Bainong Aikang 58 (AK58) and Han 6172 (H6172) by backcrossing and self-crossing combined with marker-assisted selection breeding. Adult-plant resistance of donor parent, receptor cultivars and their progeny lines were evaluated in the disease nursery fields at Mianyang, Sichuan and Yangling, Shaanxi by mixed endemic physiological races CYR32, CYR33 and CYR34. In comparison to the Chinese Spring reference genome, the flanking SSR markers Xcfa2040 (6.8 cm-Yr52) and Xbarc182 (1.2 cm-Yr52) of Yr52 were combined to search for markers of 35K SNP chip in the physical interval of target genes, and developed into derived cleaved amplified polymorphic sequences (dCAPS) and kompetitive allele specific PCR (KASP) markers. The resistance gene Yr52 was detected in BC2F5:6 progeny lines.【Result】 The evaluation of adult plant resistance and agronomic traits indicated that nineteen BC2F5:6 lines with LX987 background was obtained: Among of them, 11 were high resistance (IT=0-3, DS=1%-20%), 8 were moderate resistance (IT=4-6, DS=15%-30%), the average thousand kernel weight (TKW), kernels per spike (KPS), productive tiller number per line (PTN), plant height (PH) and spike length (SL) was 45.33 g, 46, 7, 113.26 cm and 10.05 cm, respectively. Four BC2F5:6 families with AK58 background: all showed high resistance (IT=0-3, DS=5%-25%); the average TKW, KPS, PTN, PH and SL were 44.67 g, 48, 7, 96.54 cm and 10.17 cm, respectively. Five BC2F5:6 lineages with H6172 background showed high resistance to stripe rust (IT=0-3, DS=5%-20%). The average TKW, KPS, PTN, PH and SL were 43.74 g, 49, 8, 109.72 cm and 10.06 cm, respectively. The detection rate of three simple sequence repeat (SSR) molecular markers Xbarc182, Xcfa2040 and Xwmc557 linked to Yr52, in offspring population were 78.57%, 66.67% and 66.67%, respectively. One dCAPS marker Xdcaps-Yr52-1 and one KASP marker Xkasp-Yr52-1 were successfully developed, and the detection rates were 73.68% and 41.67%, respectively. The agronomic traits of lines with high (IT=0-3) and medium (IT=4-6) resistance levels were compared. The results showed that the average TKW (P>0.05), PTN (P>0.05) and KPS (P<0.05) of lines with IT=0-3 were higher than those of families with moderate resistance level lines (IT=4-6). Five lines with disease resistance and stable agronomic traits were selected by evaluated of PH=80-105 cm, PTN≥6,KPS≥45, TKW≥42 g, SL≥8 cm.【Conclusion】 Yr52 was found to be resistant to all of the present predominant races in the adult plant stage. After introgression Yr52 into the main susceptible Chinese wheat varieties, the progeny lines with good disease resistance and agronomic characters could be used for breeding resistance varieties with multi-gene polymerization, it is enriching for the diversity of disease resistance genes and achieving durable utilization. The development of molecular markers will facilitate detect the utilization of Yr52 gene in resistance identification of germplasm in the future.

Key words: Triticum aestivum L., stripe rust, traits of yield components, stripe rust resistance, molecular markers development, molecular marker-assisted selection (MAS), backcross breeding, Yr52

Fig. 1

Frequency distributions of mean infection type and disease severity in lines of three crosses A: Infection type; B: Disease severity"

Table 1

Analysis of variance of resistant phenotypes data of BC2F5:6 and BC2F6:7 lines in 2020-2021 in Mianyang, Sichuan province and Yangling, Shaanxi province for three crosses"

家系反应型 IT of line 家系严重度 DS of lines (%)
最小 Min 最大 Max 平均 Mean 最小 Min 最大 Max 平均 Mean
绵阳MY (2020) 0 7 4 0 50 20
绵阳MY (2021) 0 7 3 0 40 10
杨凌YL (2021) 0 4 3 0 30 10
平均Mean 3 15
变异来源Source 自由度Df 均方MS 显著性a F. Sig. 自由度Df 均方MS 显著性a F. Sig.
家系 Lines 27 9.97 *** 27 421.34 ***
区组/环境Block/Environments 3 0.48 ns 3 49.07 **
环境Environments 2 19.54 *** 2 1156.58 ***
家系×环境 Lines×Environments 54 1.74 *** 54 106.67 ***
随机误差Error 81 0.42 81 12.01
遗传力H2 0.85 0.79

Table 2

Correlation analysis of resistant phenotypes data of wheat lines at two stripe rust identification locations (MY and YL) during two years (2020 and 2021)"

Resistant phenotypes
MY (2020)
MY (2021)
YL (2021)
反应型IT 绵阳MY (2020) 1
绵阳MY (2021) 0.5** 1
杨凌YL (2021) 0.6** 0.55** 1
DS (%)
绵阳MY (2020) 1
绵阳MY (2021) 0.21ns 1
杨凌YL (2021) 0.33ns 0.73** 1

Fig. 2

The technology roadmap of populations construction and screening a, b: BC1F1 and BC2F1 population (left) screened with the flanking SSR markers Xbarc182 and Xcfa2040 (right [27]). c: Recombination screen of BC2F4 plants, 51 segregating BC2F5 families used for selected in 2017-2018; In 2018-2019, the selection was continued, and SSR markers Xcfa2040 and Xbarc182 (marked with an asterisk) were used to detect plants containing target gene, and a total of 28 BC2F5:6 lines were obtained. d: The stability agronomic traits and resistance of 28 lines were evaluated in 2020 and 2021"

Table 3

Primer sequences for simple sequence repeat (SSR) markers linked to Yr52 and developed derived cleaved amplified polymorphic sequences (dCAPS) marker and kompetitive allele specific PCR (KASP) marker"

Marker type
Position (Mb)
Primer sequence (5-3)
Size (bp)
Xwmc557 728.08 F:GGTGCTTGTTCATACGGGCT 298[36]

Table 4

The number of selected progeny lines by combined with the resistant phenotypes and agronomic traits data"

Parent/progeny lines
抗病表现和农艺性状 Resistant performance and agronomic trait Yr52
DS (%)
SL (cm)
PH (cm)
PI 660057 0 0 0 41.77 43 6 128.33 +
轮选987 LX987 4 30 7.34 46.25 42 5 70.00 -
百农矮抗58 AK58 7 80 6.68 46.72 46 4 59.80 -
邯6172 H6172 6 60 7.84 53.50 43 6 68.40 -
LX987//LX987/PI660057-17 3 20 8.47 43.76 48 7 86.83 +
AK58//AK58/PI660057-20 2 10 9.11 43.26 45 8 84.67 +
AK58//AK58/PI660057-21 3 10 10.49 43.62 52 7 96.83 +
AK58//AK58/PI660057-23 3 20 9.33 47.26 47 8 99.17 +
H6172//H6172/PI660057-25 2 10 9.81 48.18 48 8 101.60 +

Fig. 3

The frequency distributions of histogram of mean agronomic characters on progeny lines of three crosses PTN: Productive tiller number per line; TKW: Thousand kernel weight; PH: Plant height; SL: Spike length; KPS: Kernels per spike. The same as below"

Fig. 4

Box plot for comparison of average agronomic traits in progenies between high and intermediate resistance *: The significant level of Student’s t test with no paired at P<0.05; ns: Not significant at P>0.05; Whiskers: 10%-90%"

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