大豆胞囊线虫抗性位点Rhg1和Rhg4优异等位变异在黄淮育成品种中的分布

doi:10.3864/j.issn.0578-1752.2019.15.001

Abstract

Abstract:

【Objective】The disease caused by soybean cyst nematode (SCN, Heterodera glycines) is an important worldwide soybean disease. Planting SCN-resistant cultivars has formed the primary basis for controlling SCN. SCN spread widely and infect heavily in the Huang-Huai Valleys almost wherever soybean is grown. This study genotyped the SCN resistance alleles distributed in Huang-Huai soybean varieties and the results will provide scientific basis for guiding the rational and effective utilization of resistant varieties. 【Method】In this study, four KASP developed from Rhg1 and Rhg4 loci were selected. The validity of selected KASP marker were verified by genotyping 16 known resistant varieties such as ZDD2315, Zhonghuang 57 and ShanxiXiaoheidou together with 3 known susceptible varieties such as Willams and Lee. Then, 170 soybean varieties cultivated from Huang-Huai Valleys, such as Yudou series, Shangdou series and Zhoudou series were genotyped. The resistance level of the varieties harboring more than two resistance alleles were tested by inoculating race 2, race 4, race 5 and new race X12 in greenhouse, respectively. These races are distributed widely Huang Huai Valley. 【Result】 There were 5 and 6 varieties harboring Rhg1-2(CC) and Rhg1-5(CC) resistance alleles, respectively. There were 6 and 7 varieties harboring Rhg4-3(TT) and Rhg4-5(CC) resistance alleles, respectively. There were 6 varieties names Kaidou 4, Shangdou 1201, Lu 0305-2, Luo 4903, Weidou12 and Weidou 91861 which harboring 2 or more resistance alleles and accounted 3.53% in tested varieties. By inoculation, the 6 varieties showed different sensitive degree to race 2, race 4 or race X12. Lu 0305-2 and Weidou 91861 showed high resistance to race 5 with FI (10.00 ± 0.48) and (7.00 ± 0.63), respectively. Shangdou 1201 showed resistance to race 5 with FI (26.20 ± 0.91). Kaidou 4, Luo 4903 and Weidou12 showed sensitive or high sensitive to race 5 with FI (35.00 ± 2.48), (64.80 ± 3.91) and (58.20 ± 2.19), respectively. 【Conclusion】The results showed that SCN-resistant alleles in Rhg1 or Rhg4 were rare in the released Huang Huai Valleys varieties. More attention should be paid to introduce SCN-resistant alleles in cultivating varieties. Combined with the dominant race of SCN distributed in Huang Huai Valley, the varieties that resistant to multiple races should be given priority in breeding. The four KASP used in this study could be used to phenotype and screen the resistant sources.

Key words: soybean, soybean cyst nematode, resistance alleles, Huang-Huai Valleys, screen resistant sources

LIAN Yun,LI HaiChao,LI JinYing,WANG JinShe,WEI He,LEI ChenFang,WU YongKang,LU WeiGuo. Distribution of Soybean Cyst Nematode Resistance Allele Rhg1 and Rhg4 in Huang-Huai Soybean Varieties[J].Scientia Agricultura Sinica, 2019, 52(15): 2559-2566.

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品种 Varieties	2号小种 Race 2		4号小种 Race 4		5号小种 Race 5		X12号小种 Race X 12
品种 Varieties	胞囊数目 No. of Cysts	胞囊指数 FI	胞囊数目 No. of Cysts	胞囊指数 FI	胞囊数目 No. of Cysts	胞囊指数 FI	胞囊数目 No. of Cysts	胞囊指数 FI
Lee68	179.40±9.57	—	195.40±6.36	—	111.40±8.08	—	380.00±18.99	—
开豆4号 Kaidou 4	299.67±23.19	167.04	234.00±14.06	119.75	35.00±2.48	31.42	263.80±6.75	69.42
商豆1201 Shangdou 1201	264.25±48.70	147.30	210.40±16.65	107.68	26.20±0.91	23.52	231.50±21.00	60.92
鲁0305-2 Lu 0305-2	311.40±38.23	173.58	185.80±7.88	95.09	10.00±0.48	8.98	186.75±20.03	49.14
漯4903 Luo 4903	223.60±19.52	124.64	237.20±17.80	121.39	64.80±3.91	58.17	231.80±20.74	61.00
潍豆12 Weidou 12	134.80±14.95	75.14	228.40±12.90	116.89	58.20±2.19	52.24	321.80±13.89	84.68
潍豆91861 Weidou 91861	192.00±12.96	107.02	108.60±7.72	55.58	7.00±0.63	6.28	206.80±18.57	54.42

重复	品种Varieties	胞囊数目No. of Cysts	胞囊指数FI
	Lee68	487.25±28.59	—
I	商豆1201 Shangdou 1201	288.13±11.18	59.13
	鲁0305-2 Lu0305-2	180.50±11.88	37.04
	潍豆91861 Weidou 91861	64.38±2.82	22.29
II	Lee68	288.86±11.78	—
	潍豆91861 Weidou 91861	52.25±1.89	18.09

生理小种 Race	接种量（虫卵/杯） Inoculation density (Eggs/cup)	Lee68	中黄57 Zhonghuang57	胞囊指数 FI
2号小种 Race 2	2000	108.00±59.87	20.20±0.80	9.44
	6000	194.20±41.90	2.40±0.51	1.24
	6000	281.60±69.90	20.60±2.40	7.32
4号小种 Race 4	2000	182.60±36.40	22.20±3.31	12.16
	6000	242.14±44.82	331.80±45.45	137.03
	6000	278.13±30.62	250.57±55.28	90.09

Distribution of Soybean Cyst Nematode Resistance Allele Rhg1 and Rhg4 in Huang-Huai Soybean Varieties

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