四川省草莓灰霉病菌对咯菌腈的抗性测定及其机制

doi:10.3864/j.issn.0578-1752.2018.22.006

Abstract

Abstract:

【Objective】Grey mold is an important disease in strawberry production, which seriously affects the yield and quality of strawberry. The objective of this study is to clarify the resistance frequency and resistance mechanism of Botrytis cinerea in different strawberry-growing areas in Sichuan Province, and to provide theoretical basis for the fungicide control of strawberry grey mold.【Method】The disease samples were collected from Chengdu, Deyang, Meishan, Leshan and Yaan in Sichuan Province from 2016 to 2017, and 188 strains of B. cinerea were isolated and purified. The sensitivity of 188 strains of B. cinerea was classified with the distinguish measurement method. The toxicity and osmotic pressure sensitivity of fludioxonil to some representative strains were assayed using the method of mycelial growth-inhibition capacity. The glycerol content of the resistant and sensitive strains treated with fludioxonil was determined by the method of glycerol-copper colorimetric assay. The sequences of type III histidine kinase gene BOS1 (BC1G_00374) in the resistant- and sensitive-fludioxonil strains were piecewise amplified and sequenced. The effects of mutations on the structure of BOS1 were predicted and evaluated by Swissmodle and I-TASSER, respectively.【Result】Of 188 strains, 8 strains showed high resistance, 9 strains showed medium resistance, 43 strains showed low resistance and the rest were sensitive. The EC₅₀ of representative strains ranged from 0.03 to 0.62 μg·mL ^-1, and the resistance multiple of the representative strains ranged from 2.2 to 45.9. The concentrations of 1.25-10 g·L ^-1 and 1.25-20 g·L ^-1 NaCl could stimulate the hypha growth of the sensitive- and resistant-fludioxonil strains, respectively, whereas the concentration of >40 g·L ^-1 inhibited the hypha growth, especially in the resistant strains, and the higher the resistant level, the stronger the inhibition rate. The glycerol content of representative strains ranged from 0.0025 to 0.0148 μg·mL ^-1 under normal conditions, and there was no significant correlation between glycerol content and fludioxonil resistance of the strain, but the glycerol content of the resistant and sensitive strains increased after the treatment of fludioxonil (0.1 μg·mL ^-1). The increase of glycerol content in resistant strains was significantly lower than that in sensitive strains. The low resistance strains YAHY-13, CDCZ-2 and medium resistance strain CDCZ-42 mutated in the TAR and HAMP regions, meanwhile the medium resistance strain CDCZ-20 and high resistance strains MYFC-10 and CDCZ-43 mutated in TAR and REC regions, whereas the mutation site of TAR region in CDCZ-20 was I365N, and which of MYFC-10 and CDCZ-43 was I365S. Different mutation positions showed different effects on the region structure of BOS1, in which the F127S, I365N, I365S, V1136I, A1259T were all in the irregular curl of BOS1 structure, but the I365N and I365S in TAR region made the overall deviation of the region structure irregular curl. 【Conclusion】In some areas of Sichuan Province, B. cinerea has developed resistance to fludioxonil. Compared with the sensitive strains, the tolerance ability of field resistant strains to osmotic pressure increased, but when the concentrations exceeded the tolerance range, they were highly sensitive to osmotic stress and the increase of glycerol content in the field resistant strains under the fludioxonil stress was significantly lower than that of the sensitive strains. The mutation position and mode of histidine kinase BOS1 are closely related to the resistance level of B. cinerea to fludioxonil.

Key words: Botrytis cinerea, fludioxonil, resistance, osmotic pressure, glycerol content, BOS1

GONG ChangWei,QIN YiMan,QU JinSong,WANG XueGui. Resistance Detection and Mechanism of Strawberry Botrytis cinerea to Fludioxonil in Sichuan Province[J].Scientia Agricultura Sinica, 2018, 51(22): 4277-4287.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2018.22.006

https://www.chinaagrisci.com/EN/Y2018/V51/I22/4277

Figures/Tables 9

Table 1

Table 2

Table 3

Table 4

Fig. 1

Table 5

Fig. 2

Fig. 3

Table 6

References 40

[1]	ROMANAZZI G, FELIZIANI E . Botrytis cinerea (gray mold)// BAUTISTA-BAÑOS S. Postharvest Decay: Control Strategies. Elsevier, 2014: 131-146.
[2]	张国珍, 钟珊 . 草莓灰霉病研究进展. 植物保护, 2018,44(2):1-10.
	ZHANG G Z, ZHONG S . Advances in strawberry gray mold. Plant Protection, 2018,44(2):1-10. (in Chinese)
[3]	WILLIAMSON B, TUDZYNSKI B, TUDZYNSKI P, VAN KAN J A . Botrytis cinerea: the cause of grey mould disease. Molecular Plant Pathology, 2007,8(5):561-580.
[4]	MYRESIOTIS C K, KARAOGLANIDIS G S, TZAVELLAKLONARI K . Resistance of Botrytis cinerea isolates from vegetable crops to anilinopyrimidine, phenylpyrrole, hydroxyanilide, benzimidazole, and dicarboximide fungicides. Plant Disease, 2007,91(4):407-413. doi: 10.1094/PDIS-91-4-0407
[5]	FERNÁNDEZ-ORTUÑO D, BRYSON P K, GRABKE A, SCHNABEL G . Monitoring for resistance in Botrytis cinerea from strawberry to seven chemical classes of fungicides in the eastern United States// APS-MSA Joint Meeting. 2013, 103(6): S2.43.
[6]	SCHIRRA M, D’AQUINO S, PALMA A, MARCEDDU S, ANGIONI A, CABRAS P, SCHERM B, MIGHELI Q . Residue level, persistence, and storage performance of citrus fruit treated with fludioxonil. Journal of Agricultural and Food Chemistry, 2005,53(17):6718-6724. doi: 10.1021/jf051004w pmid: 16104790
[7]	乔广行, 严红, 么奕清, 黄金宝, 李兴红 . 北京地区番茄灰霉病菌的多重抗药性检测. 植物保护, 2011,37(5):176-180. doi: 10.3969/j.issn.0529-1542.2011.05.035
	QIAO G H, YAN H, YAO Y Q, HUANG J B, LI X H . Detection of multiple fungicide resistance in Botrytis cinerea from tomato in Beijing. Plant Protection, 2011,37(5):176-180. (in Chinese) doi: 10.3969/j.issn.0529-1542.2011.05.035
[8]	BARDAS G A, VELOUKAS T, KOUTITA O, KARAOGLANIDIS G S . Multiple resistance of Botrytis cinerea from kiwifruit to SDHIs, QoIs and fungicides of other chemical groups. Pest Management Science, 2010,66(9):967-973. doi: 10.1002/ps.1968 pmid: 20730988
[9]	张玮, 乔广行, 黄金宝, 王忠跃, 李兴红 . 中国葡萄灰霉病菌对嘧霉胺的抗药性检测. 中国农业科学, 2013,46(6):1208-1212. doi: 10.3864/j.issn.0578-1752.2013.06.014
	ZHANG W, QIAO G H, HUANG J B, WANG Z Y, LI X H . Evaluation on resistance of grape gray mold pathogen Botrytis cinerea to pyrimethanil in China. Scientia Agricultura Sinica, 2013,46(6):1208-1212. (in Chinese) doi: 10.3864/j.issn.0578-1752.2013.06.014
[10]	徐建强, 平忠良, 刘莹, 马世闯, 许道超, 杨岚, 郑伟, 刘圣明, 夏彦飞, 林晓民 . 咯菌腈对四种牡丹叶片病原真菌的抑制活性. 中国农业科学, 2017,50(20):4036-4045. doi: 10.3864/j.issn.0578-1752.2017.20.018
	XU J Q, PING Z L, LIU Y, MA S C, XU D C, YANG L, ZHENG W, LIU S M, XIA Y F, LIN X M . Inhibitory activity of fludioxonil to four pathogenic fungi of peony leaves. Scientia Agricultura Sinica, 2017,50(20):4036-4045. (in Chinese) doi: 10.3864/j.issn.0578-1752.2017.20.018
[11]	FURUKAWA K, RANDHAWA A, KAUR H, MONDAL A K, HOHMANN S . Fungal fludioxonil sensitivity is diminished by a constitutively active form of the group III histidine kinase. FEBS Letters, 2012,586(16):2417-2422. doi: 10.1016/j.febslet.2012.05.057 pmid: 22687241
[12]	LAWRY S M, TEBBETS B, KEAN I, STEWART D, HETELLE J, KLEIN B S . Fludioxonil induces Drk1, a fungal group III hybrid histidine kinase, to dephosphorylate its downstream target, Ypd1. Antimicrobial Agents and Chemotherapy, 2017,61(2):e01414-16. doi: 10.1128/AAC.01414-16 pmid: 27872062
[13]	VIAUD M, FILLINGER S, LIU W, POLEPALLI JS, LE PÊCHEUR P, KUNDURU AR, LEROUX P, LEGENDRE L . A class III histidine kinase acts as a novel virulence factor in Botrytis cinerea. Molecular Plant-Microbe Interactions, 2006,19(9):1042-1050.
[14]	SEGMÜLLER N, ELLENDORF U, TUDZYNSKI B, TUDZYNSKI P . BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea. Eukaryotic Cell, 2007,6(2):211-221. doi: 10.1128/EC.00153-06 pmid: 1797955
[15]	LIU W, LEROUX P, FILLINGER S . The HOG1-like MAP kinase Sak1 of Botrytis cinerea is negatively regulated by the upstream histidine kinase Bos1 and is not involved in dicarboximide- and phenylpyrrole-resistance. Fungal Genetics and Biology, 2008,45(7):1062-1074. doi: 10.1016/j.fgb.2008.04.003 pmid: 18495505
[16]	PARKINSON J S . Signaling mechanisms of HAMP domains in chemoreceptors and sensor kinases. Annual Review of Microbiology, 2010,64:101-122. doi: 10.1146/annurev.micro.112408.134215 pmid: 20690824
[17]	AIROLA M V, WATTS K J, BILWES A M, CRANE B R . Structure of concatenated HAMP domains provides a mechanism for signal transduction. Structure, 2010,18(4):436-448. doi: 10.1016/j.str.2010.01.013 pmid: 2892831
[18]	VIGNUTELLI A, HIBER-BODMER M, HIBER U W . Genetic analysis of resistance to the phenylpyrrole fludioxonil and the dicarboximide vinclozolin in Botryotinia fuckeliana (Botrytis cinerea). Mycological Research, 2002,106(3):329-335.
[19]	FERNÁNDEZORTUÑO D, BRYSON P K, GRABKE A, SCHNABEL G . First report of fludioxonil resistance in Botrytis cinerea from a strawberry field in Virginia. Plant Disease, 2013,97(6):848-849. doi: 10.1094/PDIS-01-13-0012-PDN
[20]	武东霞 . 灰葡萄孢菌(Botrytis cinereal)对苯噻菌酯和咯菌睛的抗药性风险研究[D]. 南京: 南京农业大学, 2015.
	WU D X . Resistance risk for benzothiostrobin and fludioxonil against Botrytis cinerea[D]. Nanjing: Nanjing Agricultural University, 2015. (in Chinese)
[21]	YOSHIDA H, ANO H, ISHIDA C, TANIGAWA N, KIKUI M, TAKASHIMA T, TSUYUGUCHI I . A study of INH 0.1 microgram/ml resistant M. tuberculosis strains assessed by BrothMIC MTB-1 method. Kekkaku(Tuberculosis), 2002,77(7):533-535. pmid: 12187818
[22]	慕立义 . 植物化学保护研究方法. 北京: 中国农业出版社, 1994.
	MU L Y. Research Methods of Plant Chemical Protection. Beijing: China Agriculture Press, 1994. ( in Chinese)
[23]	赵建江, 张小风, 马志强, 王文桥, 韩秀英 . 番茄灰霉病菌对咯菌腈的敏感基线及其与不同杀菌剂的交互抗性. 农药, 2013,52(9):684-685.
	ZHAO J J, ZHANG X F, MA Z Q, WANG W Q, HAN X Y . Baseline-sensitivity of Botrytis cinerea on tomato to fludioxonil and cross-resistance against diverse fungicides. Agrochemicals, 2013,52(9):684-685. (in Chinese)
[24]	仇骏, 王大兵, 黄得庆 . 甘油铜比色法测定水中甘油的含量方法研究. 中国化工贸易, 2014,30(6):140. doi: 10.3969/j.issn.1674-5167.2014.30.125
	QIU J, WANG D B, HUANG D Q . Determination of glycerin in water by glycerol copper colorimetric method. China Chemical Trade, 2014,30(6):140. (in Chinese) doi: 10.3969/j.issn.1674-5167.2014.30.125
[25]	DUAN Y B, GE C G, LIU S G, CHEN C J, ZHOU M G . Effect of phenylpyrrole fungicide fludioxonil on morphological and physiological characteristics of Sclerotinia sclerotiorum. Pesticide Biochemistry and Physiology, 2013,106(1/2):61-67. doi: 10.1016/j.pestbp.2013.04.004
[26]	ROBERT X, GOUET P . Deciphering key features in protein structures with the new ENDscript server. Nucleic Acids Research, 2014,42(Web Server Issue):W320-W324. doi: 10.1093/nar/gku316 pmid: 24753421
[27]	LI X, FERNÁNDEZ-ORTUÑO D, GRABKE A, SCHNABEL G . Resistance to fludioxonil in Botrytis cinerea isolates from blackberry and strawberry. Phytopathology, 2014,104(7):724-732. doi: 10.1094/PHYTO-11-13-0308-R pmid: 24423402
[28]	LIU S, HAI F, JIANG J . Sensitivity to fludioxonil of Botrytis cinerea isolates from tomato in Henan Province of China and characterizations of fludioxonil-resistant mutants. Journal of Phytopathology, 2017,165(2):98-104. doi: 10.1111/jph.12542
[29]	禾丽菲, 陈乐乐, 肖斌, 赵时峰, 李秀环, 慕卫, 刘峰 . 番茄叶霉病菌对咯菌腈敏感基线的建立及田间防治效果评价. 中国农业科学, 2018,51(8):1475-1483.
	HE L F, CHEN L L, XIAO B, ZHAO S F, LI X H, MU W, LIU F . Establishment of sensitivity baseline and evaluation of field control efficacy of fludioxonil against Fulvia fulva. Scientia Agricultura Sinica, 2018,51(8):1475-1483. (in Chinese)
[30]	SANG C, REN W, WANG J, XU C, ZHANG Z H, ZHOU M G, CHEN C J, WANG K . Detection and fitness comparison of target-based highly fludioxonil-resistant isolates of Botrytis cinerea, from strawberry and cucumber in China. Pesticide Biochemistry and Physiology, 2018,147:110-118. doi: 10.1016/j.pestbp.2018.01.012 pmid: 29933980
[31]	REN W C, SHAO W Y, HAN X, ZHOU M G, CHEN C J . Molecular and biochemical characterization of laboratory and field mutants of Botrytis cinerea resistant to fludioxonil. Plant Disease, 2016,100(7):1414-1423. doi: 10.1094/PDIS-11-15-1290-RE
[32]	LI J L, WU F C, ZHU F X . Fitness is recovered with the decline of dimethachlon resistance in laboratory-induced mutants of Sclerotinia sclerotiorum after long-term cold storage. The Plant Pathology Journal, 2015,31(3):305-309. doi: 10.5423/PPJ.OA.04.2015.0066 pmid: 4564156
[33]	ZHANG Y, LAMM R, PILLONEL C, LAM S, XU J R . Osmoregulation and fungicide resistance: the Neurospora crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue. Applied and Environmental Microbiology, 2002,68(2):532-538. doi: 10.1128/AEM.68.2.532-538.2002 pmid: 11823187
[34]	KOJIMA K, TAKANO Y, YOSHIMI A, TANAKA C, KIKUCHI T, OKUNO T . Fungicide activity through activation of a fungal signalling pathway. Molecular Microbiology, 2004,53(6):1785-1796. doi: 10.1111/j.1365-2958.2004.04244.x pmid: 15341655
[35]	HOHMANN S . Osmotic stress signaling and osmoadaptation in yeasts. Microbiology and Molecular Biology Reviews, 2002,66(2):300-372. doi: 10.1128/MMBR.66.2.300-372.2002
[36]	CHEN R E, THOMER J . Function and regulation in MAPK signaling pathways: lessons learned from the yeast Saccharomyces cerevisae. Biochimica et Biophysica Acta, 2007,1773(8):1311-1340.
[37]	FILLINGER S, AJOUZ S, NICOT P C, LEROUX P, BARDIN M . Functional and structural comparison of pyrrolnitrin- and iprodione- induced modifications in the class III histidine-kinase Bos1 of Botrytis cinerea. PLoS ONE, 2012,7(8):e42520. doi: 10.1371/journal.pone.0042520 pmid: 22912706
[38]	YANG Y, LI M X, DUAN Y B, LI T, SHI Y Y, ZHAO D L, ZHOU Z H, XIN W J, WU J, PAN X Y, LI Y J, ZHU Y Y, ZHOU M G . A new point mutation in β₂-tubulin confers resistance to carbendazim in Fusarium asiaticum. Pesticide Biochemistry and Physiology, 2018,145:15-21.
[39]	尚岩 . 桃、樱桃灰霉病菌对七种杀菌剂的抗药性研究[D]. 武汉: 华中农业大学, 2016.
	SHANG Y . Study on resistance of Botrytis cinerea from peach and cherry to seven fungicides[D]. Wuhan: Huazhong Agricultural University, 2016. (in Chinese)
[40]	贾娇, 苏前富, 孟玲敏, 张伟, 李红, 刘婉丽, 晋齐鸣 . 禾谷镰孢菌对咯菌腈的抗药性诱导及对不同药剂的交互抗性//中国植物病理学会会议论文集, 2015: 553.
	JIA J, SU Q F, MENG L M, ZHANG W, LI H, LIU W L, JIN Q M . The resistance of Fusarium graminearum to fludioxonil and the interaction resistance to different medicaments// Proceeding of the Chinese Society of Plant Pathology, 2015: 553. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

采样地区 Sampling area	采样时间 Sampling time	经纬度 Latitude and longitude	菌株编号 Strain code	菌株数 Strain number
CDCZ1（成都崇州市Chongzhou, Chengdu）	2017-02-16	103o41′, 30o34′	CDCZ (1-15)	52
CDCZ2（成都崇州市Chongzhou, Chengdu）	2017-02-16	103o37′, 30o36′	CDCZ (16-30)
CDCZ3（成都崇州市Chongzhou, Chengdu）	2017-02-16	103o44′, 30o40′	CDCZ (31-52)
CDSL（成都双流市Shuangliu, Chengdu）	2016-04-10	103o35′, 30o14′	CDSL (1-9)	9
CDPZ（成都彭州市Pengzhou, Chengdu）	2017-01-05	104o10′, 30o59′	CDPZ (1-10)	10
DYGH（德阳广汉市Guanghan, Deyang）	2017-03-02	104o20′, 31o3′	DYGH (1-11)	11
MSRS1（眉山仁寿县Renshou, Meishan）	2017-03-14	104o5′, 29o33′	MSRS (1-10)	26
MSRS2（眉山仁寿县Renshou, Meishan）	2017-03-14	104o4′, 29o31′	MSRS (11-26)	26
MSDP1（眉山东坡区Dongpo, Meishan）	2017-03-14	102o36′, 29o30′	MSDP (1-8)	18
MSDP2（眉山东坡区Dongpo, Meishan）	2017-03-22	103o36′, 30o0′	MSDP (9-18)	18
LSJY1（乐山井研县Jingyan, Leshan）	2017-03-14	104o1′, 29o24′	LSJY (1-4)	11
LSJY2（乐山井研县Jingyan, Leshan）	2017-03-14	103o34′, 29o18′	LSJY (5-11)	11
YAHY（雅安汉源县Hanyuan, Yaan）	2017-03-25	102o36′, 29o30′	YAHY (1-16)	16
MYFC（绵阳涪城区Fucheng, Mianyang）	2017-03-27	104o49′, 31o20′	MYFC (1-17)	17
MYJY（绵阳江油市Jiangyou, Mianyang）	2017-03-27	104o44′, 31o47′	MYJY (1-18)	18

引物 Primer	序列 Sequence
BFl	TACCGATCGAAAAACCCAAC
BRl	TGGGCTGGTCTCTCAATCTT
BF2	CAACGTTATGGCACAAAATCTCA
BR2	AAGTTTCTGGCCATGGTGTTCA
BF3	GGTCGGAACTGATGGAACTC
BR3	CGCGGTAAGTGAGGTCTAGG
BF4	GCAAACCGTATGATCATGGA
BR4	AGCTCGATTCTCCAAAGCAG
BF5	TCCCGTTATTCATGTCAGCTT
BR5	AAGTACTCGCAGTCGGTGGT

地区 Area	菌株数 Strain number	敏感菌株数 Number of sensitive strains	敏感菌株比例 Proportion of sensitive strains (%)	低抗菌株数 Number of low resistance strains	低抗菌株比例 Proportion of low resistance strains (%)	中抗菌株数Number of medium resistance strains	中抗菌株比例Proportion of medium resistance strains (%)	高抗菌株数Number of high resistance strains	高抗菌株比例Proportion of high resistance strains (%)
CDCZ	52	37	70.59	8	15.69	4	7.84	3	5.88
CDSL	9	8	88.89	1	11.11	0	0	0	0
CDPZ	10	3	30.00	6	60.00	1	10.00	0	0
DYGH	11	6	54.55	4	36.36	1	9.09	0	0
MSRS	26	20	76.92	3	11.54	2	7.69	1	3.85
MSDP	18	16	88.88	2	11.11	0	0	0	0
LSJY	11	6	54.54	5	45.45	0	0	0	0
YAHY	16	9	56.25	7	43.75	0	0	0	0
MYFC	17	10	58.82	2	11.76	1	5.88	4	23.53
MYJY	18	13	72.22	5	27.78	0	0	0	0
总计Total	188	128	68.08	43	22.87	9	4.78	8	4.25

菌株编号 Strain code	回归方程 Regression equation (y=a+bx)			EC₅₀（95%置信区间） 95% confidence interval	抗性倍数 Resistance multiple
菌株编号 Strain code	a	b	r	EC₅₀（95%置信区间） 95% confidence interval	抗性倍数 Resistance multiple
CDCZ-2	5.7333	0.7442	0.9011	0.10 (0.06-0.17)	7.4
CDCZ-11	6.5566	1.1984	0.9801	0.05 (0.03-0.08)	3.7
CDCZ-20	5.8440	0.7696	0.9631	0.08 (0.05-0.12)	5.9
CDCZ-42	6.4192	1.4323	0.8460	0.10 (0.07-0.14)	7.4
CDCZ-43	5.4371	1.1802	0.8918	0.43 (0.27-0.68)	31.9
CDPZ-8	6.2100	0.8161	0.9945	0.03 (0.02-0.06)	2.2
YAHY-13	6.5496	1.6160	0.9865	0.11 (0.09-0.14)	8.1
MYFC-10	5.1480	0.7054	0.9456	0.62 (0.31-1.23)	45.9

菌株编号 Strain code	未加咯菌腈No fludioxonil added	加入0.1 μg·mL^-1咯菌腈1 mL Add 1 mL fludioxonil (0.1 μg·mL^-1)
CDCZ-2	0.0067±0.0008f	0.0143±0.0001c
CDCZ-11	0.0108±0.0001d	0.0300±0.0012e
CDCZ-20	0.0058±0.0002b	0.0099±0.0001b
CDCZ-42	0.0070±0.0005bc	0.0109±0.0001b
CDCZ-43	0.0081±0.0002c	0.0096±0.0001b
CDPZ-8	0.0077±0.0015g	0.0246±0.0001d
YAHY-13	0.0025±0.0002a	0.0057±0.0001a
MYFC-10	0.0148±0.0003e	0.0155±0.0016c

Resistance Detection and Mechanism of Strawberry Botrytis cinerea to Fludioxonil in Sichuan Province

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 9

References 40

Related Articles 15

Metrics

Comments

Recommended 0

菌株编号 Strain code	抗性水平 Resistance level	突变点 Discontinuity	突变结构域 Mutant domain
YAHA-13	低抗 Low resistance	127 F-S 287 V-G 365 I-N	— HAMP TAR
CDCZ-2	低抗 Low resistance	365 I-S 287 V-G 1136 V-I (+) 1259 A-T	TAR HAMP REC —
CDCZ-42	中抗 Medium resistance	127 F-S 287 V-G 365 I-N	— HAMP TAR
CDCZ-20	中抗 Medium resistance	365 I-N 1136 V-I (+) 1259 A-T	TAR REC —
MYFC-10	高抗 High resistance	365 I-S 1136 V-I (+) 1259 A-T	TAR REC —
CDCZ-43	高抗 High resistance	365 I-S 1136 V-I (+) 1259 A-T	TAR REC —

[1]	ZHANG XiaoLi, TAO Wei, GAO GuoQing, CHEN Lei, GUO Hui, ZHANG Hua, TANG MaoYan, LIANG TianFeng. Effects of Direct Seeding Cultivation Method on Growth Stage, Lodging Resistance and Yield Benefit of Double-Cropping Early Rice [J]. Scientia Agricultura Sinica, 2023, 56(2): 249-263.
[2]	SHAO ShuJun,HU ZhangJian,SHI Kai. The Role and Mechanism of Linoleyl Ethanolamide in Plant Resistance Against Botrytis cinerea in Tomato [J]. Scientia Agricultura Sinica, 2022, 55(9): 1781-1789.
[3]	LIU Jiao,LIU Chang,CHEN Jin,WANG MianZhi,XIONG WenGuang,ZENG ZhenLing. Distribution Characteristics of Prophage in Multidrug Resistant Escherichia coli as well as Its Induction and Isolation [J]. Scientia Agricultura Sinica, 2022, 55(7): 1469-1478.
[4]	GUO ZeXi,SUN DaYun,QU JunJie,PAN FengYing,LIU LuLu,YIN Ling. The Role of Chalcone Synthase Gene in Grape Resistance to Gray Mold and Downy Mildew [J]. Scientia Agricultura Sinica, 2022, 55(6): 1139-1148.
[5]	YAN LeLe,BU LuLu,NIU Liang,ZENG WenFang,LU ZhenHua,CUI GuoChao,MIAO YuLe,PAN Lei,WANG ZhiQiang. Widely Targeted Metabolomics Analysis of the Effects of Myzus persicae Feeding on Prunus persica Secondary Metabolites [J]. Scientia Agricultura Sinica, 2022, 55(6): 1149-1158.
[6]	WANG Kai,ZHANG HaiLiang,DONG YiXin,CHEN ShaoKan,GUO Gang,LIU Lin,WANG YaChun. Definition and Genetic Parameters Estimation for Health Traits by Using on-Farm Management Data in Dairy Cattle [J]. Scientia Agricultura Sinica, 2022, 55(6): 1227-1240.
[7]	ZHANG YaLing, GAO Qing, ZHAO Yuhan, LIU Rui, FU Zhongju, LI Xue, SUN Yujia, JIN XueHui. Evaluation of Rice Blast Resistance and Genetic Structure Analysis of Rice Germplasm in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2022, 55(4): 625-640.
[8]	WANG MengRui, LIU ShuMei, HOU LiXia, WANG ShiHui, LÜ HongJun, SU XiaoMei. Development of Artificial Inoculation Methodology for Evaluation of Resistance to Fusarium Crown and Root Rot and Screening of Resistance Sources in Tomato [J]. Scientia Agricultura Sinica, 2022, 55(4): 707-718.
[9]	XIANG MiaoLian, WU Fan, LI ShuCheng, WANG YinBao, XIAO LiuHua, PENG WenWen, CHEN JinYin, CHEN Ming. Effects of Melatonin Treatment on Resistance to Black Spot and Postharvest Storage Quality of Pear Fruit [J]. Scientia Agricultura Sinica, 2022, 55(4): 785-795.
[10]	HU ChaoYue, WANG FengTao, LANG XiaoWei, FENG Jing, LI JunKai, LIN RuiMing, YAO XiaoBo. Resistance Analyses on Wheat Stripe Rust Resistance Genes to the Predominant Races of Puccinia striiformis f. sp. tritici in China [J]. Scientia Agricultura Sinica, 2022, 55(3): 491-502.
[11]	TANG ZiYun,HU JianXin,CHEN Jin,LU YiXing,KONG LingLi,DIAO Lu,ZHANG FaFu,XIONG WenGuang,ZENG ZhenLing. Relationship Between Biofilm Formation and Molecular Typing of Staphylococcus aureus from Animal Origin [J]. Scientia Agricultura Sinica, 2022, 55(3): 602-612.
[12]	LI ZhiLing,LI XiangJu,CUI HaiLan,YU HaiYan,CHEN JingChao. Development and Application of ELISA Kit for Detection of EPSPS in Eleusine indica [J]. Scientia Agricultura Sinica, 2022, 55(24): 4851-4862.
[13]	ZHANG Qi,DUAN Yu,SU Yue,JIANG QiQi,WANG ChunQing,BIN Yu,SONG Zhen. Construction and Application of Expression Vector Based on Citrus Leaf Blotch Virus [J]. Scientia Agricultura Sinica, 2022, 55(22): 4398-4407.
[14]	DU JinXia,LI YiSha,LI MeiLin,CHEN WenHan,ZHANG MuQing. Evaluation of Resistance to Leaf Scald Disease in Different Sugarcane Genotypes [J]. Scientia Agricultura Sinica, 2022, 55(21): 4118-4130.
[15]	FENG AiQing,WANG CongYing,ZHANG MeiYing,CHEN Bing,FENG JinQi,CHEN KaiLing,WANG WenJuan,YANG JianYuan,SU Jing,ZENG LieXian,CHEN Shen,ZHU XiaoYuan. Pathotype Analysis of Xanthomonas oryzae pv. oryzae in Main Rice Producing Regions of China and Establishment of Differential Hosts of Near-Isogenic Lines [J]. Scientia Agricultura Sinica, 2022, 55(21): 4175-4195.