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    16 November 2018, Volume 51 Issue 22
    CONTENTS
    Scientia Agricultura Sinica. 2018, 51(22):  0-0.  doi:
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    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    The Evaluation of Cd Accumulation in Grains of Different Wheat Materials
    MING Yi,ZHANG XiZhou,YU HaiYing
    Scientia Agricultura Sinica. 2018, 51(22):  4219-4229.  doi:10.3864/j.issn.0578-1752.2018.22.001
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    【Objective】 The screening of the low-Cd accumulation genotypes for wheat not only benefits for the safe production of Cd-contaminated agricultural soils, but also provides breeding materials for the study of hereditary characteristics of wheat. 【Method】 In this study, 139 wheat lines were considered to be the research objectives and two consecutive field trials were carried out under Cd heavy pollution in soil in 2014-2015 and 2015-2016. Cd concentrations in grains of the 139 wheat lines for the two field trials were evaluated to investigate Cd accumulation difference among the 139 wheat lines, so as to obtain wheat lines with the characteristics of low-Cd accumulation in grains. Followly, the characteristics of Cd accumulation and yield of the low-Cd accumulation wheat materials were explored under Cd heavy pollution in soil by a pot experiment. With the index of Cd concentration in grain and grain yield, cluster analysis was also carried out to obtain superior wheat lines with low-Cd accumulation and high yield. The difference of Cd accumulation and translocation in organs between the two wheat lines was analyzed to get further understanding of the mechanisms of low-Cd accumulation in grains of the low-Cd accumulation wheat lines.【Result】For the two field experiments in 2014-2015 and 2015-2016, the range and coefficient of variation (CV) of Cd concentrations in grains of the 139 wheat materials were 0.002-0.271 mg·kg -1 DW -1, 63.097% and 0.095-0.343 mg·kg -1 DW -1, 24.960%, respectively. There was a significant difference for Cd concentrations in grains among the 139 wheat materials. According to the cluster analysis, 16 low-Cd accumulation wheat lines were obtained for the two field experiments, including 12N551 (M033), JIMAI22 (M059), SHUMAI375 (M075), ZHONGLIANG22 (M079), 30389 (M092), B7094 (M094), ML2652 (M114), G219-24 (M116), 77782 (M121), NANNONGOzy23 (M123), SHENGC90097 (M124), Pm99915-1 (M126), NANNONG02y39 (M127), SHENGCXK027-4 (M129), 02Y23 (M131) and LIANGMAI No.2 (M139). For the pot experiment, significant difference for Cd concentrations in grains and grain yield among the 16 low-Cd accumulation wheat lines was observed. Cd concentrations in grains and grain yield among the 16 low-Cd accumulation wheat lines ranged from 0.286-0.910 mg·kg -1, 3.199-7.716 g·plant -1 and 0.881-1.381 mg·kg -1, 3.075-8.252 g·plant -1, respectively. when exposed to 1 mg·kg -1 and 4 mg·kg -1 Cd, The CV of Cd concentrations in grains and grain yield among the 16 low-Cd accumulation wheat lines was 33.706%, 24.044% and 12.276%, 12.276%, respectively. Then, the dominant wheat materials (12N551 (M033), ZHONGLIANG22 (M079), G219-24 (M116) and LIANGMAI No. 2 (M139) ) with low-Cd content and high yield were obtained according to the cluster analysis. Compared with the high-Cd accumulative materials (KANGXIU3816 (M060) and SHE1136 (M073)), Cd concentrations in different organs of the low-Cd accumulation wheat lines (12N551 (M033), ZHONGLIANG22 (M079), G219-24 (M116) and LIANGMAI No. 2 (M139) ) exposed to 4 mg·kg -1 Cd were significantly higher than those exposed to 1 mg·kg -1 Cd. Cd concentrations in grains of the low-Cd accumulation wheat lines (12N551 (M033), ZHONGLIANG22 (M079), G219-24 (M116) and LIANGMAI No. 2 (M139) ) were significantly lower than the high-Cd accumulation wheat lines(KANGXIU3816 (M060), SHE1136 (M073)). The low-Cd accumulation wheat lines presented lower Cd translocation from straws to grains and therefore lower Cd accumulation in grains. 【Conclusion】 According to the two-year field experiments and pot experiment, (12N551 (M033), ZHONGLIANG22 (M079), G219-24 (M116) and LIANGMAI No. 2 (M139)) showed lower Cd accumulation and greater grain yield in grains under different experimental conditions of field and pot experiments in 2014-2017, thus could be considered to be ideal candidates for the cultivation in the moderate Cd-contaminated agricultural soils and also providing materials for the study of genetic characteristics of the low Cd accumulation in grains.

    Functional Characterization of AtNEK6 Overexpression in Cotton Under Drought and Salt Stress
    FAN Xin,ZHAO LeiLin,ZHAI HongHong,WANG Yuan,MENG ZhiGang,LIANG ChengZhen,ZHANG Rui,GUO SanDui,SUN GuoQing
    Scientia Agricultura Sinica. 2018, 51(22):  4230-4240.  doi:10.3864/j.issn.0578-1752.2018.22.002
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    【Objective】 AtNEK6 is a NIMA related kinase in Arabidopsis thaliana. Overexpression of AtNEK6 in Arabidopsis can promote plant growth, and improve the salt tolerance and drought tolerance of plants. By transforming AtNEK6 into cotton, the molecular mechanism of its resistance to stress was studied, so as to provide theoretical basis and germplasm resources for breeding new cotton varieties with drought tolerance and salinity tolerance.【Method】The AtNEK6 gene was introduced into cotton by the Agrobacterium transformation method, and the expression level of AtNEK6 in transgenic lines was analyzed by real-time PCR. The growth and development of transgenic cotton were observed by observing the phenotype of transgenic plants and observing epidermal cells by scanning electron microscope. Mannitol and NaCl were used to simulate drought tolerance and drought tolerance of transgenic cotton by simulated drought treatment and salt treatment. The contribution of AtNEK6 to the stress tolerance of transgenic cotton was identified by measuring related physiological indexes.【Result】The transgenic seedlings were screened by Kanamycin, and 10 different transgenic lines were identified by PCR. qRT-PCR analysis was used to select L7, L17 and L25 with higher expression levels. Under normal conditions, the transgenic lines were exhibited higher height and larger leaf than wild-type plants. But the cell surface area of transgenic cotton leaves was not significantly different from that of wild type by scanning electron microscope. The expressions of cell cycle related genes CYCB1, 1 and CYCA3, 1 and growth related genes GhGRF5, GhEOD, GhAN3 and GhEBP1 were upregulated in the transgenic lines. Salt and drought tolerance of transgenic cotton was analyzed. On the normal 1/2MS medium, the root length, fresh weight and dry weight of transgenic lines were not significantly different from those of wild type, and the number of lateral roots increased. However, in medium containing 250 mmol·L -1 mannitol, the root length, the lateral root number, fresh weight and dry weight of transgenic lines were significantly higher than those of wild type, showing a better growth state. In medium containing 200 mmol·L -1 NaCl, the number of lateral roots, fresh weight and dry weight of the transgenic lines were significantly higher than those of the wild type. Cotton seedlings of 30 days normal growth in the greenhouse were treated with 300 mmol·L -1 mannitol, the SOD activity of the transgenic plants was increased by 0.65 times, 0.42 times, 1.45 times compared with the wild type, and the CAT activity was increased by 0.65 times, 0.64 times, 0.42 times, and the MDA content was decreased. 0.51 times, 0.41 times, 0.22 times. Similarly, the changes of physiological indexes in transgenic lines in 250 mmol·L -1 NaCl treatment were higher than the ones in WT. In addition, the expression levels of related stress responsive genes GhAREB, GhDREB, GhNCEDGhLEA5 in transgenic cotton were significantly higher than those in wild type cotton, which further showed that overexpression of AtNEK6 in cotton could increase salt tolerance and drought tolerance of plants.【Conclusion】AtNEK6 promotes the growth of cotton by participating in the regulation of cell cycle and growth. At the same time, it improved the salt tolerance and drought tolerance of cotton in adversity.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY
    Effects of Drought Stress on Photosynthetic Characteristics of Wheat Near-Isogenic Lines with Different Wax Contents
    YANG YanHui,MA Xiao,ZHANG ZiShan,GUO Jun,LI YueNan,LIANG Ying,SONG JianMin,ZHAO ShiJie
    Scientia Agricultura Sinica. 2018, 51(22):  4241-4251.  doi:10.3864/j.issn.0578-1752.2018.22.003
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    【Objective】 The objective of this paper was to investigate the effects of different drought levels on the photosynthetic characteristics of wheat with different wax contents. 【Method】 A pair of wheat near-isogenic lines (NILs), including high-wax line JM205 and low-wax line JM204, were used as experimental materials to simulate the process of soil moisture change in the field by means of gradual drought stress in the artificial climate chamber. The two wheat near-isogenic lines with different waxy contents were planted in the same pot to ensure the same drought degree. With the prolongation of drought treatment, the relative soil water content decreased gradually. Water potential, photosynthetic gas exchange parameters and fluorescence parameters of wheat flag leaf under different soil water contents were measured. 【Result】 Under low water stress (60%-49% of relative soil water content), there was no significant difference in photosynthetic rate (Pn) between flag leaf of JM205 and JM204, however, the photosynthetic rate of both wax and waxy lines decreased gradually, but the less waxy line JM204 showed a greater decline. Under moderate drought stress (Relative soil water content between 49% and 32%), the high-wax plant had higher water potential and larger stomatal opening than that of the low-wax one, so the CO2 supply was sufficient and the photosynthetic rate was higher. Under moderate drought stress, the PSII actual photochemical efficiency (ΦPSⅡ) and maximum photochemical efficiency (Fv/Fm) decreased more rapidly in the low-wax line, which indicated that PSII electron transfer inhibition and light inhibition were more serious. The JIP-test analysis found that blocked PSII electron transport was mainly due to QA to QB receptor-side electron transfer restrictions. In contrast, the high-wax line maintained relatively higher photosynthetic capacity under drought conditions, with a smoother electron transfer and less excess light energy, resulting in less light suppression. When relative soil water content dropped below 32%, the water potential and photosynthetic capacity of both NILs decreased significantly and there was no significant difference between them. 【Conclusion】 This study preliminarily improved understanding of the physiological mechanism of leaf wax improving drought tolerance of plants, and the high-wax line JM205 has significant drought resistance in the soil moisture range of 49%-32%, providing theoretical basis for selection and cultivation of drought-resistant wheat varieties.

    Cellulose Deposition Characteristics of High Strength Cotton Fiber and Optimal Temperature Requirements in Xinjiang Region
    TIAN JingShan,ZHANG XuYi,HU XiaoBing,SUI LongLong,ZHANG PengPeng,WANG WenMin,GOU Ling,ZHANG WangFeng
    Scientia Agricultura Sinica. 2018, 51(22):  4252-4263.  doi:10.3864/j.issn.0578-1752.2018.22.004
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    【Objective】 Xinjiang region is a major cotton growing-region in northwest China. Temperatures in the region, especially night-time temperatures, drop drastically at the end of the cotton growing season. The objective of research was to explicate the effects of temperatures on fiber development, so as to benefit to cotton production. 【Method】 Three field experiments were conducted by different sowing dates, elevated night-time temperature during entire fiber development (from anthesis to boll opening) and elevated night-time temperature during different stage of fiber development (from anthesis to the onset of rapid cellulose deposition, between the onset and termination of rapid cellulose deposition, and from the termination of rapid cellulose deposition to boll opening). The effects of temperatures on cellulose deposition were analyzed to explore the temperature conditions for producing high fiber strength. 【Result】 Fiber strength was significantly affected by the duration of rapid cellulose deposition (T), the average rate of cellulose deposition from anthesis until the onset of rapid cellulose deposition (V1) and the maximum cellulose content (Wm). Fiber strength was quadratically related to the V1 and was positively correlated with the Wm. Growing degree days was the factor associated with cellulose deposition in cotton fiber. The relationship between both was going to go the other way during the different fiber developing stages. During the early stage of fiber development (anthesis to the onset of rapid cellulose deposition), growing degree days was significantly and negatively correlated with fiber strength, whereas there was positive relationship between both during the period between the onset and termination of rapid cellulose deposition. During the period from anthesis until the onset of rapid cellulose deposition, the average rate of cellulose deposition decreased as growing degree days increased, which had an unfavorable about fiber strength. During the period of rapid cellulose deposition, growing degree days increased significantly the cellulose deposition rate during from anthesis until the onset of rapid cellulose deposition and the duration time of rapid cellulose deposition, then fiber strength increased. There was a possibility of producing more than 30 cN/tex of fiber strength, when the following conditions were met: (1) the V1 was between 1.32%·d -1and1.76%·d -1; (2) cellulose deposition entered the onset of rapid cellulose deposition (t1) at 6.7-13.3 days post-anthesis (dpa); (3) the duration of rapid cellulose deposition (T) was 20.2-25.6 days;(4) the time of cellulose deposition was 39.0-46.9 days, and (5) the growing degree days was 5.6℃-96.3℃ and 181.5℃-262.3℃ during from anthesis until the onset of rapid cellulose deposition and the duration time of rapid cellulose deposition, respectively. 【Conclusion】 Therefore, growing degree days resulted in different effects on fiber strength during the different fiber developing, and the main reason was that growing degree days caused variability effects on the average cellulose rate during the period from anthesis until the onset of rapid cellulose deposition.

    Effects of Planting Pattern and Density on Population Structure and Yield of Sorghum
    XIAO JiBing,LIU Zhi,KONG FanXin,XIN ZongXu,WU HongSheng
    Scientia Agricultura Sinica. 2018, 51(22):  4264-4276.  doi:10.3864/j.issn.0578-1752.2018.22.005
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    【Objective】 The combination of planting pattern and planting density was one of the effective ways to increase the utilization rate of light energy and to increase the yield of crops in dryland farming area, which was of great significance in dryland agricultural production. The effects of different planting densities and planting modes on the structure of sorghum canopy were studied to provide a theoretical basis for further excavating the yield potential of sorghum in the semi-arid region of western Liaoning. 【Method】 The brewed sorghum variety Liaoza 19 was used as the experimental material. Two factors split plot design was used to examine the effects of different treatments on photosynthetic characteristics of the population and yield by analyzing plant shape index, photosynthetic physiological indexes, and aboveground biomass. The main area was planted with 60 cm equal row spacing (P1) and 80 cm+40 cm wide-narrow row planting (P2), and the split area was planting density with 75 000 plants/hm 2(D1), 105 000 plants/hm 2(D2), 135 000 plants/hm 2(D3) and 165 000 plants/hm 2(D4). The experiment was repeated three times. 【Result】 In the two years, grain yield of sorghum was in turn D3>D2>D4>D1 from big to small under the same planting pattern. The average 2-year yield of P2D2 was 5.02% higher than that of P1D2, and that of P2D3 was 6.96% higher than that of P1D3, and that of P2D1 was 0.27% lower than that of P1D1, respectively. The yield of P2D4 was 2.55% lower than that of P1D4 in 2017. The yield of P2D3, which was the highest in all treatments, was 10 267.14 kg·hm -2. In accordance with the increase of planting density, the plant height, population leaf area index and leaf orientation value showed an increasing trend, while stem diameter, stem diameter coefficient, leaf area per plant, angle between leaf and stem, light transmittance, SPAD value and net photosynthetic rate decreased. The wide-narrow row planting had the advantages in the aspects of stem diameter coefficient, group leaf area index, light transmittance, net photosynthetic rate, SPAD value compared with equidistant row planting under D2 and D3. P2D2 increased by 2.80%, 3.17%, 16.33% compared with P1D2 and P2D3 increased by 9.29%, 7.27%, 17.57% compared with P1D3 respectively in the aspects of mean stem diameter coefficient, mean population leaf area index and mean net photosynthetic rate in the two years. In the stage of flowering and milking, P2D2 increased by 22.55%, 15.81% compared with P1D2, respectively, and P2D3 increased by 37.45%, 102.09% compared with P1D3 in the aspect of mean light transmittance at the bottom, respectively, and P2D2 increased by 38.72%, 8.16% compared with P1D2, respectively, and P2D3 increased by 56.59%, 93.60% compared with P1D3 in the aspect of mean light transmittance in the middle of the canopy, respectively, in the two years. In the stage of flowering and milking, P2D2 increased by 6.46%, 5.41% compared with P1D2, respectively, and P2D3 increased by 8.75%, 5.46% compared with P1D3 on two years' average SPAD value, respectively. Under the density of D2 and D3, the upper leave of the wide-narrow row planting was relatively straight and leaf area was lesser, which could improve the light receiving condition of the middle and lower leaves, the lower blade was relatively flat and leaf area was larger, which could reduce the loss of light leakage and improve the utilization ratio of light energy compared with equidistant row planting in the two years. 【Conclusion】Appropriate increase of planting density was the key to increase sorghum yield. Under optimum planting density, wide-narrow row planting, which was an important way to realize the synergistic gain and yield enhancement of crop population structure and plant individual function, could effectively improve canopy light transmittance, increase population leaf area index, expand photosynthetic area, and improve the photosynthetic performance of the leaves, especially the middle and lower leaves.

    PLANT PROTECTION
    Resistance Detection and Mechanism of Strawberry Botrytis cinerea to Fludioxonil in Sichuan Province
    GONG ChangWei,QIN YiMan,QU JinSong,WANG XueGui
    Scientia Agricultura Sinica. 2018, 51(22):  4277-4287.  doi:10.3864/j.issn.0578-1752.2018.22.006
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    【Objective】Grey mold is an important disease in strawberry production, which seriously affects the yield and quality of strawberry. The objective of this study is to clarify the resistance frequency and resistance mechanism of Botrytis cinerea in different strawberry-growing areas in Sichuan Province, and to provide theoretical basis for the fungicide control of strawberry grey mold.【Method】The disease samples were collected from Chengdu, Deyang, Meishan, Leshan and Yaan in Sichuan Province from 2016 to 2017, and 188 strains of B. cinerea were isolated and purified. The sensitivity of 188 strains of B. cinerea was classified with the distinguish measurement method. The toxicity and osmotic pressure sensitivity of fludioxonil to some representative strains were assayed using the method of mycelial growth-inhibition capacity. The glycerol content of the resistant and sensitive strains treated with fludioxonil was determined by the method of glycerol-copper colorimetric assay. The sequences of type III histidine kinase gene BOS1 (BC1G_00374) in the resistant- and sensitive-fludioxonil strains were piecewise amplified and sequenced. The effects of mutations on the structure of BOS1 were predicted and evaluated by Swissmodle and I-TASSER, respectively.【Result】Of 188 strains, 8 strains showed high resistance, 9 strains showed medium resistance, 43 strains showed low resistance and the rest were sensitive. The EC50 of representative strains ranged from 0.03 to 0.62 μg·mL -1, and the resistance multiple of the representative strains ranged from 2.2 to 45.9. The concentrations of 1.25-10 g·L -1 and 1.25-20 g·L -1 NaCl could stimulate the hypha growth of the sensitive- and resistant-fludioxonil strains, respectively, whereas the concentration of >40 g·L -1 inhibited the hypha growth, especially in the resistant strains, and the higher the resistant level, the stronger the inhibition rate. The glycerol content of representative strains ranged from 0.0025 to 0.0148 μg·mL -1 under normal conditions, and there was no significant correlation between glycerol content and fludioxonil resistance of the strain, but the glycerol content of the resistant and sensitive strains increased after the treatment of fludioxonil (0.1 μg·mL -1). The increase of glycerol content in resistant strains was significantly lower than that in sensitive strains. The low resistance strains YAHY-13, CDCZ-2 and medium resistance strain CDCZ-42 mutated in the TAR and HAMP regions, meanwhile the medium resistance strain CDCZ-20 and high resistance strains MYFC-10 and CDCZ-43 mutated in TAR and REC regions, whereas the mutation site of TAR region in CDCZ-20 was I365N, and which of MYFC-10 and CDCZ-43 was I365S. Different mutation positions showed different effects on the region structure of BOS1, in which the F127S, I365N, I365S, V1136I, A1259T were all in the irregular curl of BOS1 structure, but the I365N and I365S in TAR region made the overall deviation of the region structure irregular curl. 【Conclusion】In some areas of Sichuan Province, B. cinerea has developed resistance to fludioxonil. Compared with the sensitive strains, the tolerance ability of field resistant strains to osmotic pressure increased, but when the concentrations exceeded the tolerance range, they were highly sensitive to osmotic stress and the increase of glycerol content in the field resistant strains under the fludioxonil stress was significantly lower than that of the sensitive strains. The mutation position and mode of histidine kinase BOS1 are closely related to the resistance level of B. cinerea to fludioxonil.

    Effects of Colorful Rice on the Degree of Occurrence and Damage by Cnaphalocrocis medinalis and Parasitic Behavior of Its Egg Parasitoids
    ZHENG XuSong,TIAN JunCe,HOU JianJun,LÜ ZhongXian
    Scientia Agricultura Sinica. 2018, 51(22):  4288-4296.  doi:10.3864/j.issn.0578-1752.2018.22.007
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    【Objective】The application of colorful rice in field painting is an important part of creative agriculture, but it may affect the occurrence and damage of the main pests in paddy field. The objective of this study is to investigate the effects of colorful rice on Cnaphalocrocis medinalis and its egg parasitic wasps, and to provide a theoretical basis for the control of C. medinalis in creative agriculture.【Method】The biological characteristics of C. medinalis in purple rice, yellow rice and common rice (control) were compared, including the developmental duration of larval and pupal stage, survival rate of larvae and pupae, pupal weight and sex ratio. The oviposition selectivity of C. medinalis, and parasitic selectivity of its important egg parasitoids (Trichogramma chilonis and Trichogramma japonicum) between colorful rice and common rice were also compared. The occurrence of C. medinalis in colorful rice paddy field and creative field painting with purple rice was investigated as well.【Result】The results of laboratory test showed that C. medinalis preferred to lay eggs on colorful rice, and the percentage of egg laying on colorful rice was more than 60%, which was significantly higher than that of green common rice. The ecological fitness of C. medinalis on colorful rice was higher than that on green common rice. The larval and pupae survival rates of C. medinalis fed with purple rice and yellow rice were significantly higher than those of common rice. The colorful rice had no significant effect on the developmental duration, pupal weight and sex ratio of C. medinalis. The parasitic selectivity of different Trichogramma species to the eggs of C. medinalis on different rice varieties was different. T. chilonis preferred to parasitize the C. medinalis eggs laid on purple rice, and had the lowest parasitic selectivity on yellow rice, but the parasitic rate had no significant difference. However, T. japonicum preferred to parasitize the eggs laid on green common rice, and had the lowest selectivity of the eggs laid on purple rice. The parasitic rate of T. japonicum on the eggs of common rice was 4.3 times higher than that of purple rice and 2.1 times higher than that of yellow rice when three kinds of rice existed at the same time. The rate of rolled leaf caused by C. medinalis and the larva density in colorful rice field were significantly higher than those in common rice field. The rate of rolled leaf in purple rice area was the highest (15.3%), which was 10.9 times of that of common rice and 3 times of that of yellow rice. The residual rate of C. medinalis in purple rice and yellow rice was 4.5% and 3.3%, respectively, which was significantly higher than that of common rice (1.1%). When purple rice was used to write Chinese characters in paddy field, the rate of rolled leaf in purple rice was above 6%, which was significantly higher than that in common rice field (below 4%). 【Conclusion】Compared with common rice, colorful rice is easier to attract C. medinalis, and the ecological fitness of C. medinalis on colorful rice is higher than that of common rice, while T. japonicum has the lowest selectivity to C. medinalis eggs on purple rice. It is unfavorable to biological control of C. medinalis on purple rice. The negative ecological effects should be fully considered when using colorful rice to paint the creative agricultural paddy fields, and corresponding control strategies should be worked out.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Quality Control of Soil Map Database at 1:50 000 Scale in China
    XU AiGuo,ZHANG RenLian,TIAN YouGuo,JI HongJie,ZHANG HuaiZhi,LONG HuaiYu
    Scientia Agricultura Sinica. 2018, 51(22):  4297-4305.  doi:10.3864/j.issn.0578-1752.2018.22.008
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    【Background】 Soil large data with time and space dimension, could provide data support for research work and policy decision in agriculture, environment, and land management. The soil survey maps and reports are the base on building soil time and space database. Because of the large differences of soil mapping standards between different areas and different periods, quality control is necessary for developing high quality soil database from non-standard soil maps and soil survey reports. 【Objective】 The purpose of this study was to analyze the status of paper soil maps, to identify the key issues of quality control in its digital integration database, and to explore the corresponding technical standards and specifications, so as to provide quality guarantee for large scale soil map database. 【Method】 By analyzing the situation of soil maps and soil survey reports and combining related research for precision control of digital topographic map in China, precision requirements suitable for digitizing soil map were developed. After comparing soil type names from county level soil maps and soil standard names in national standard, soil names, especially soil great group names, were revised in soil map database and every soil name was encoded. 【Result】 For building soil map database from different sources large scale soil maps in large area, the quality control included the geographic precision, standardization of digital soil map elements, integration of soil map database, and coding elements type for the whole database. The contents of geographic precision included scanning precision of paper maps, coordinate system, geometric correction precision, as well as collection and numbers of control points. Standardization of map elements extraction mainly included the collection of soil elements and non-soil elements, classification and code of digital element types, and standard of layer name and map sheets. The integration for whole database included revision of soil types, code of soil types and non-soil types, and standard of boundary by using uniform boundary. In the process of soil data integration and harmonization, revising soil names, especially revising soil great group names and coding, was the mainly quality control method. According to national standard and soil profile records in soil survey reports of county, province and national level for reference, more than 300 non-standard soil great group names were revised to 60 national standard names. By hierarchical code method, a unique code for each soil type in the entire database was achieved with five-level coding of soil order, soil great group to soil species. Using uniform boundary, the boundaries of adjacent counties were seamless. Based on the integration of the entire soil special database, the conversion were achieved from counties map sheet to international standard map sheet at 1:50 000. 【Conclusion】 The quality control is very important in building soil map database of large area by using non-standard soil maps from different region and period. The key of quality control were geographic precision, standardization of digital soil map elements, integration of soil map database and coding soil types for the entire database. The quality control methods and corresponding indicators for these three issues conformed to the actual situation of soil survey maps in China. Using the above methods, the 1:50 000 soil map database had a total 13 240 standard map sheets of 1:50 000 scale, and involving 1 688 counties and county farms. In addition, we discussed the status of this database and prospected the future direction for expansion, in the hope that to provide a data basis for the study of the long-term soil properties data mining by combining the physical and chemical properties of the soil.

    Effects of Biochar and Other Amendments on the Cabbage Growth and Soil Fertility in Yellow-Brown Soil and Red Soil
    LÜ Bo,WANG YuHan,XIA Hao,YAO ZiHan,JIANG CunCang
    Scientia Agricultura Sinica. 2018, 51(22):  4306-4315.  doi:10.3864/j.issn.0578-1752.2018.22.009
    Abstract ( 374 )   HTML ( 8 )   PDF (403KB) ( 332 )   Save
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    【Objective】A pot experiment of cabbage was conducted to investigate the various effects of 3 soil amendments (biochar (C), potassium humate (HA-K) and lime (CaO)) on plant growth and soil fertility of yellow-brown soil and red soil, so as to provide information for reasonable use of soil amendments.【Method】The present research set 4 different treatments in 2 different types of soil to study biomass, nutrient content, soluble protein and malondialdehyde content of cabbage and pH, available nutrients content, exchange-aluminum (Al) content and enzyme activity in soil.【Result】The results showed that: (1) compared with control treatment, the application of biochar and lime promoted the growth and stress-tolerance of cabbage in yellow-brown soil and red soil, in consequence of improved cabbage yield, nitrogen (N) and potassium (K) content, soluble protein content, and reduced malondialdehyde content in leaf. However, after HA-K application, the cabbage yield in yellow-brown soil was increased to 25.93 g/plant, while only 0.18 g/plant in red soil; (2) The effects of the 3 amendments on soil fertility differed in yellow-brown soil and red soil were different. Biochar application increased soil pH, available phosphorus (P), available K, organic matter, activities of urease and acid phosphatase, which improved soil fertility of the two types of soil. In addition, the supply of C significantly decreased alkaline hydrolysis N and exchange-Al content in soil. The soil pH and organic matter content in yellow-brown soil increased by 1.39 units and 168.4%, respectively, and the exchange-Al content decreased by 89.3%, while the pH value and organic matter content in red soil increased by 0.82 and 775.6%, respectively, and the exchange-Al content decreased by 93.9%. What was more, the application of HA-K and CaO significantly increased soil pH and invertase activity, and reduced exchange-Al content in both two types of soil. However, there were no significant effects of HA-K on available P, available K, organic matter, urease and acid phosphatase activities, and exchange-Al content, while significantly reduced the content of alkali-hydrolyzed N. The application of CaO only reduced soil alkaline N, available P and organic matter.【Conclusion】Generally, All of the 3 different soil amendments had great effects on the growth of cabbage and soil fertility in two different types of soil. Lime and biochar were recommended soil amendments, which could improve not only the soil fertility but also the yield of cabbage, and the application effect of humate potassium in yellow brown soil was better than that in red soil.

    The Factors of Farmland Conversion and Its Temporal and Spatial Characteristics: An Integrated Model
    CUI XuFeng,MA YunMeng,ZHANG GuangHong
    Scientia Agricultura Sinica. 2018, 51(22):  4316-4327.  doi:10.3864/j.issn.0578-1752.2018.22.010
    Abstract ( 306 )   HTML ( 12 )   PDF (1354KB) ( 242 )   Save
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    【Objective】The purpose of this paper was to reveal the temporal and spatial characteristics of the factors affecting farmland conversion, and to provide decision-making information support for policy making for the protection and utilization of farmland.【Method】Based on the panel data of farmland conversion from 2006 to 2015, an integrated model of "ordinary regression model-panel model-geographically weighted regression-geographically and temporally weighted regression" (abbreviately named “OPGT” ) was established to analyze the factors of farmland conversion【Result】The ordinary regression model, GWR and GTWR model results showed that urban population growth, fixed asset investment, economy, arable and industrial structure variables all passed the significance test; Moran's I of farmland conversion was 0.740, and passed significance test at the 1% level. The results showed that there was a significant positive spatial correlation of farmland conversion. Ordinary regression model, GWR and GTWR models were used to estimate the equations, and the fit goodness of the equations were 0.689, 0.785 and 0.858, respectively. The interpretation ability of GWR and GTWR models was improved significantly under the condition of adding spatio-temporal weight information. The results of GWR and GTWR models showed that the elastic coefficients of factors were spatio-temporal non-stationary. The results of spatial analysis showed that the influence of urban population growth and farmland resource endowment on farmland conversion was declining from west to east in longitude direction, and reversed U-shaped curve in latitude direction. The influence of fixed assets investment and level of economic development was increasing from west to east in longitude direction, and U-shaped curve in latitude direction. The influence of industrial structure was increasing from west to east in longitude direction, and declining from north to south in latitude direction. From the perspective of temporal evolution, the coefficients of urban population growth, fixed assets investment and level of economic development had a downward trend, while coefficients of farmland resource endowment tended to increase. Coefficients of industrial structure had been reduced in some provinces.【Conclusion】(1) OPGT was an organic whole, each part was mutually tested and complementary, which could describe the spatio-temporal effect of factors in more detail. (2) In terms of the overall action intensity of the factors, the largest elastic coefficient was industrial structure, followed by level of economic development, fixed asset investment and farmland resource endowment, and the smallest was urban population growth. (3) In terms of the spatial characteristics of factor intensities, the influence of urban population growth and farmland resource endowment on farmland conversion was declining from Western China to Eastern China, while fixed assets investment, level of economic development and industrial structure increasing. (4) From the perspective of temporal evolution, the influence of urban population growth, fixed assets investment and level of economic development on farmland conversion had a downward trend. The relationship between farmland resource endowment and farmland conversion tended to strengthen. Although the influence of industrial structure had been reduced in some provinces, its degree of overall influence was still relatively high.

    HORTICULTURE
    Cloning and Expression Analysis of BoSPx in Brassica oleracea
    WANG YuKui,BAI XiaoJing,LIAN XiaoPing,ZHANG HeCui,LUO ShaoLan,PU Min,ZUO TongHong,LIU QianYing,ZHU LiQuan
    Scientia Agricultura Sinica. 2018, 51(22):  4328-4338.  doi:10.3864/j.issn.0578-1752.2018.22.011
    Abstract ( 324 )   HTML ( 12 )   PDF (2107KB) ( 199 )   Save
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    【Objective】Self-incompatibility (SI) is a genetic barrier to inhibit self-pollination and promote hybridization in flowering plants. Here, cloning of a novel gene co-responsive to SI and calcium production during pollination, and spatio-temporal specific expression analysis of the novel gene BoSPx under self-pollination conditions, screening its interaction proteins were conducted to explore. The responding mechanism of BoSPx to self-pollination stimulated stigma in order to provide some further insights into SI process in Brassica oleracea.var.Capitata.【Method】BoSPx was cloned by using transcriptome sequencing, self-pollination and cross-pollination differential screening, and PCR cloning. Amino acid sequence alignment and conserved domain analysis were performed by DNAMAN and Smart software. Expasy online software was used to predict BoSPx protein molecular weight, isoelectric point, secondary structure and transmembrane domain. Phylogenetic tree that constructed by the neighboring method in MEGA6.0 software was used to speculate on the function of BoSPx protein after self-pollination. RT-PCR and qRT-PCR were used to detect BoSPx tissue-specific expression and the relative expression of BoSPx after self-pollination and cross-pollination. The BoSPx-GFP expression vector was constructed and the subcellular localization of BoSPx was observed under confocal microscopy; The interaction proteins were searched by using yeast two-hybrid system. 【Result】A novel gene, which contains a single exon without any introns, named BoSPx was cloned. The open reading frame of BoSPx is 396 bp, encodes a protein with 131 amino acid residues. BoSPx is a hydrophilic protein, no signal peptide and transmembrane, and the theoretical isoelectric point is 4.54. Conserved domains analysis found BoSPx contains three conserved EF-hand motifs (48-60, 64-80, and 81-96). About 500 bp up-stream of BoSPx translation start code contains an auxin response element. RT-PCR analysis found that BoSPx was expressed highest in stigma, BoSPx was also expressed in sepals, leaves, anthers and petals in flowering stage. The BoSPx expression levels in both self-pollinated and cross-pollinated stigma showed “up-down-up” expression pattern. Moreover, The expression of BoSPx in the stigma of self-pollination and cross-pollination increased at first and then decreased down to the highest expression level in stigma in flowering stage. The expression of BoSPx increased rapidly after self-pollination at 15 min and then decreased sharply so that result in SI progress. The decreased value of BoSPx was 1-2 days before flowering. Subcellular location analysis found that BoSPx expression in both the nucleus and cytoplasm. Yeast two-hybrid system did not detect interaction between BoSPx and SRK and ARC1, but BoSPx interacted with auxin family proteins BoSAUR71 and BoPID. 【Conclusion】BoSPx is highly expressed by self-pollination, which may be an auxin-regulated calcium-binding protein, which has a common response to SI and calcium. The protein has multi-tissue expression and nuclear and cytoplasmic properties, indicating that BoSPx may be involved in unknown signaling pathways other than the SRK-ARC1-ExO70A1 pathway.

    Cloning and Function Analysis of Apple Gibberellin Oxidase Gene MdGA2ox8
    LI FeiHong,HOU YingJun,LI XueHan,YU XinYi,QU ShenChun
    Scientia Agricultura Sinica. 2018, 51(22):  4339-4351.  doi:10.3864/j.issn.0578-1752.2018.22.012
    Abstract ( 388 )   HTML ( 7 )   PDF (1159KB) ( 279 )   Save
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    【Objective】The objective of this study is to clone the open reading frame (ORF) of a gibberellin 2-oxidase gene (gibberellin2-oxidase 8, GA2ox8) from apple cultivar ‘Sushuai’, to analyze its sequence characteristics and tissue expression specificity. The effects of overexpression of MdGA2ox8 on tobacco growth and development were studied in order to provide a theoretical reference for the functional analysis and application of the gene.【Method】The ORF region of MdGA2ox8 was cloned from apple by RT-PCR method. The amino acid sequence alignment and conserved domain analysis were performed by NCBI, DNAMAN and Pfam online software. The composition, theoretical molecular weight and isoelectric point (pI) of the amino acid were deduced by Expasy software online. The protein signal peptide and transmembrane domain were analyzed by SignalP and TMHMM Server V.2.0. The phylogenetic tree was constructed by a neighbor-joining (NJ) method using MEGA 7.0 program. The expression level in different tissues of apple was detected by RT-qPCR. To characterize the function of MdGA2ox8, MdGA2ox8 ORF driven by the 35S promoter was delivered into tobacco by Agrobacterium-mediated transformation approach. Transgenic plants with hygromycin resistance were obtained and identified by using GUS staining, genomic PCR, and RT-PCR. After transplanting, the plant height, internode length, blade aspect ratio, content of chlorophyll, and content of GA1, GA4 in tobacco were measured at the first flowering stage. The expression level of related genes in tobacco was analyzed by RT-qPCR.【Result】The ORF sequence of MdGA2ox8 obtained from ‘Sushuai’ is 1 122 bp in length, encoding a putative protein about 373 amino acids. The predicted molecular weight of MdGA2ox8 is 42.8 kD, the theoretical pI is 5.44 and the instability coefficient is 49.73. MdGA2ox8 contains conserved domains DIOX_N and 20G-Fell_Oxy, without obvious hydrophobic region, transmembrane domain and signal peptide. The MdGA2ox8 protein is a non-secreted protein. Phylogenetic analysis showed that the MdGA2ox8 protein was closely related to Pyrus bretschneideri GA2ox8 protein. The RT-qPCR results showed that MdGA2ox8 was differentially expressed in apple tissues, with the highest expression in flowers, followed by old leaf>young leaf>phloem, but almost no expression in xylem and fruitlet. Five transgenic positive lines were obtained by transferring MdGA2ox8 into model plant tobacco. Compared with the wild-type, the GA4 content decreased and GA1 content increased in overexpressed MdGA2ox8 tobacco plants. The average content of GA1 in wild-type and transgenic tobacco was 1.26 and 1.75 ng·g -1, respectively. The average content of GA4 in wild-type and transgenic tobacco was 5.43 and 1.07 ng·g -1, respectively. Compared with the wild-type, the total content of GA4 and GA1 in transgenic tobacco plants decreased, resulting in shorter internode length, dwarfing and delayed flowering. The average height of wild-type and transgenic tobacco plants was 38.50 and 7.36 cm, respectively. The average internode length of tobacco plants was 9.5 and 3.3 cm, respectively. The leaf of transgenic tobacco was dark green and the leaf aspect was reduced. Moreover, the expression level of endogenous gibberellin synthesis pathway-related genes NtGA3ox1 and NtGA3ox2 was positively regulated by MdGA2ox8 in transgenic tobacco.【Conclusion】The ORF of gibberellin 2-oxidase gene MdGA2ox8 was obtained. The difference of tissue expression in MdGA2ox8 was found. The total content of GA1 and GA4 in MdGA2ox8 overexpressed tobacco decreased, which resulted in the shortening of internode length and dwarfing of plants.

    FOOD SCIENCE AND ENGINEERING
    Qualitative and Quantitative Detection Methods of Pork in Beef and Its Chinese Processing Products
    ZHU Yang,LIU YongFeng,WEI YanChao,SHEN Qian,WANG YiFan
    Scientia Agricultura Sinica. 2018, 51(22):  4352-4363.  doi:10.3864/j.issn.0578-1752.2018.22.013
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    【Objective】 The objective of this paper was to establish a qualitative and quantitative detection method for pork components in beef and Chinese processed products, so as to guarantee the purity of beef products. 【Method】 The pig genomic DNA from pork and different processed pork products were extracted, and then effects of processing methods on pig DNA quality, sensitivity and detection limit were analyzed through DNA quality testing, PCR amplification and sensitivity test. The raw beef was prepared, and the dried, steamed, boiled fried, stewed and roasted beef products were mixed with the binary mixed meat of different proportions (10%, 5%, 1%, 0.1%) of pork, and then the qualitative and quantitative detections of common PCR and real-time PCR were carried out. The application of DNA in adulteration identification. 【Result】 The DNA quality test results of different processing methods showed that different processing methods significantly affected the purity of DNA (P<0.05). The DNA purity (A260nm/A280nm) ranged from 1.893 to 1.977 in raw pork and seven kinds of pork products, which were higher than the theoretical value of 1.8. The DNA content ranged from 110 to 277 μg·g -1, and the DNA content of the processed pork products was significantly higher than that of the raw pork treatment group (P<0.05); Agarose electrophoresis showed that the DNA of raw pork and seven kinds of meat products was seriously degraded after 6 months of storage, but raw pork still obtained some unclear long-segment DNA, and the pig DNA of seven meat products all degraded into small fragments of DNA, indicating that long-term placement and heat treatment significantly affect the integrity of pig DNA; Although the degradation of DNA in pork products was serious, the mitochondrial genes were amplified by ordinary PCR, and the PCR products of all samples were presented as clear and single bands. It could be seen that the DNA extracted from the processed meat product could be tested for sensitivity and adulteration; The sensitivity test results showed that the common PCR was highly sensitive. The 10-fold gradient dilution showed that the minimum detection limit of pig DNA extracted from the eight test group samples was 0.005 ng. The standard curve formed by fluorescence quantitative PCR amplification of pig DNA was also formed, which had a good linear relationship. The slope of the standard curve was between -3.1 and -3.7, the coefficient of determination R 2 was greater than 0.99, the PCR amplification efficiency was between 89% and 100%, and the quantitative PCR could be detected 0.005 ng of pig DNA. Qualitative quantitative PCR test results of adulterated samples showed that the minimum detection limit of qualitative test for mixed raw meat and other six mixed meat products was 0.1% except for fried mixed meat (1%), indicating that ordinary PCR could detect trace amounts of pork composition. In the quantitative test of mixed meat, the coefficient of determination (R 2) of the standard curve of eight test groups established according to different adulteration ratios was more than 0.99, and the slope was -3.1--3.6. Each curve had a good linear relationship and could realize beef with quantitative detection of medium pork components. Comparing the quantitative results of raw meat and meat products, there was a difference of about 0.1 to 0.6 cycles between the intercepts of the standard curve of mixed raw meat and mixed meat products. 【Conclusion】 Different processing could significantly affect the content, purity and integrity of DNA in meat, but it did not affect the detection limit and sensitivity of DNA in meat products. Both ordinary PCR and quantitative PCR could detect the micro-content of adulterated meat. It could be seen that the detection method based on PCR technology had high sensitivity, high speed and high specificity, and the quantitative detection standard curve had high linear correlation and amplification efficiency, which could provide reliable quality control and inspection plan for meat industry and verification label declaration. The results could be applied to some commercial samples to ensure the purity of meat products.

    ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT
    Effects of the Daily Cycle Variation of the Moderate Ambient Temperatures on the Serum Brain Gut Peptide and the Diversity of Caecal Microflora in Broilers
    CHANG ShuangShuang,LI Meng,LI XiuMei,SHI YuXiang,ZHANG MinHong,FENG JingHai
    Scientia Agricultura Sinica. 2018, 51(22):  4364-4372.  doi:10.3864/j.issn.0578-1752.2018.22.014
    Abstract ( 276 )   HTML ( 12 )   PDF (487KB) ( 226 )   Save
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    【Objective】Stress can alter the function of brain - gut axis and the structure of intestinal flora, among which heat stress is one of the most important environmental factors affecting the health of livestock and poultry. This study was carried out to investigate the effects of the daily cycle variation of the moderate ambient temperatures on the serum brain gut peptide and the diversity of caecal microflora in broilers, and to provide a theoretical basis for the healthy broiler breeding mode. 【Method】 The study included trials with Arbor Acres (AA) broilers. At the 22d, 120broilers with equal average body weights were selected, randomly divided into 2treatments × 6 replicates of 10 birds each(male and female half and half) . The pretest period lasted for 7 days and broilers were kept at 21℃ and 60% relative humidity. When the chickens were aged 29 days, formal tests began. The environmental temperature was changed by 24h cycle (26 -29, -32, -26, -29), and the relative humidity was set to 35%, 60%, 85%, respectively. Both kept constant until the end of the experiment. The trial period lasted for 14 days. On day 21 and 42, one birds from each replicate were randomly selected and killed. The broiler was sampled with jugular vein. The contents of 5-HT, VIP and CCK in serum of broilers were measured with Elisa kit. Meanwhile cecal contents were aseptically collected, placed in a centrifugal tube, rapidly frozen in liquid nitrogen, and stored at -80℃. The effect of different humidity on serum brain gut peptide and bacterial diversity in the ceacal digesta of broilers at the daily cycle variation of the moderate ambient temperatures was studied by using 16S r DNA-based denaturing gradient gel electrophoresis (DGGE). 【Result】(1) At the 7d , the content of Serum 5-HT at the daily cycle variation of the moderate ambient temperatures was higher than control group (P<0.05); the content of serum 5-HT and VIP in the control group and were no significant difference; at the 14d, the content of serum VIP in broilers at the daily cycle variation of the moderate ambient temperatures was lower than the control group (P<0.05); the content of serum 5-HT and SP in the control group and were no significant difference. (2)At the 7d, the bacteria bands at the daily cycle variation of the moderate ambient temperatures were lower than the control group; at the 14d, the bacteria bands at the daily cycle variation of the moderate ambient temperatures and the control group were same, but broiler cecal microflora diversity decreased at the daily cycle variation of the moderate ambient temperatures. (3) Common microflora of broiler cecum included Clostridium josui, Bacteroides vulgatus, Parabacteroides johnsonii, Bacteroides dorei and Ruminococcus flavefaciens; The daily cycle variation of the moderate ambient temperatures was beneficial to the growth of Parabacteroides johnsonii and Bacteroides dorei, and inhibited the colonization of Intestinimonas butyriciproducen and Subdoligranulum variabile.【Conclusion】Compared with the control group, at the 7d, the content of serum 5-HT in broilers increased at the daily cycle variation of the moderate ambient temperatures , and at the 7d, the serum VIP content in broilers decreased at the daily cycle variation of the moderate ambient temperatures , and there was no significant difference in SP between the two groups; The daily cycle variation of the moderate ambient temperatures affected the cecal microflora structure and diversity in broilers.

    RNA-seq Analysis on Development Arrest of Duck Pectoralis Muscle During Semi-Late Embryonic Period
    LIU HongXiang,XU WenJuan,ZHU ChunHong,TAO ZhiYun,SONG WeiTao,ZHANG ShuangJie,LI HuiFang
    Scientia Agricultura Sinica. 2018, 51(22):  4373-4386.  doi:10.3864/j.issn.0578-1752.2018.22.015
    Abstract ( 352 )   HTML ( 10 )   PDF (1210KB) ( 294 )   Save
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    【Objective】 In order to find the molecular varying mechanism involving the development arrest of pectoralis, Chinese native breeds, including Gaoyou Duck (GYD) and Jinding Duck (JDD), were selected for RNA-seq study using the pectoralis samples during the semi-late embryonic period.【Method】3 ducks of GYD and JDD , respectively, in the 21th embryonic day (ed21) and ed27 were selected to collect pectoralis major muscle and to extract total RNA to build cDNA library for RNA-seq with HiseqTM2000 of Illumina. At last, bioinformatics methods were used to extract differentially expressed genes (DEGs) between different breeds and time points, and to analyze the gene function annotation for studying molecular mechanism of pectoralis development retardation during ed21 and ed27. 【Result】 The results showed that the base ratios with quality value higher than 20 in reads (Q20) were more than 94%, and the base ratios of Q30 were more than 89%, which indicated a reliable sequencing result for the following analysis. The RNA level correlation inspection and mRNA expression level cluster graph both manifest that the correlation of mRNA expression patterns of GYD and JDD at ed21 or ed27 were higher than that of GYD (JDD) during ed21 and ed27. The numbers of DEGs between ed21 and ed27 (6128 DEGs for GYD and 6452 DEGs for JDD) were both apparently more than the numbers of DEGs between GYD and JDD in ed21 (522 DEGs) and ed27 (299 DEGs). qRT-PCR results of selected genes showed a strong correlation with RNA-seq results. GO and KEGG enrichment analysis showed the results that the genes involved in energy metabolism (mainly was coenzyme Q related genes, ATP enzymic synthesis related genes, and cytochrome C related genes) up regulated and DNA replication and cell cycle related-genes (mainly was minichromosome maintenance complex related genes and replication factor C related genes) down regulated significantly. This varies of related genes expression might relate to the slow myoblast proliferation and gradually exit the cell cycle to prepare for the next stage of fusing to multi-nuclei myotube and form myofiber. In the key genes involving in muscle growth and development, IGF1 (a gene promoting muscle growth) and MyoG (inducing terminal differentiation of myoblast) both down regulated dramatically. However, MUSTN1 gene, accelerating muscle fibre into differentiation and fusing stage, and MyoD gene, promoting muscle progenitor cell differentiated to myoblast, were expressed in a higher level in ed27 than in ed21. 【Conclusion】 A lot of genes differentially expressed between ed21 and ed27 in pectoralis muscle of duck, among of which the up-regulation of energy metabolism related genes, the down-regulation of DNA replicate and cell cycle related genes, and up-regulated MUSTN1, down-regulated IGF1 and MyoG, might closed relate to arrest phenomenon of pectoralis development during the semi-late stage of duck embryos.

    Research and Application of Honeybee Non-Grafting Larvae Technology
    ZHANG Bo,WU XiaoBo,LIAO ChunHua,HE XuJiang,YAN WeiYu,ZENG ZhiJiang
    Scientia Agricultura Sinica. 2018, 51(22):  4387-4394.  doi:10.3864/j.issn.0578-1752.2018.22.016
    Abstract ( 631 )   HTML ( 11 )   PDF (2932KB) ( 305 )   Save
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    【Objective】 In the beekeeping industry, beekeepers need to conduct artificial larvae grafting either for royal jelly harvesting or for rearing queens artificially. Artificially grafting larvae requires beekeepers to have good eyesight and high proficiency. Especially with the increase of labor cost and the aging of beekeepers, artificially grafting larvae is a technical limitation that needs to be solved in the production of beekeeping. Under the support of the National Bee Industry Technology System for ten consecutive years, our team has been engaged in the research of non-grafting larvae technology. The objective of this study is to remove the limitation of artificially grafting larvae and to provide technical support for scientific beekeeping.【Method】Based on the biological characteristics of bees and the principle of bionics, a food-grade plastic worker comb foundation with regular holes was designed. The holes in the plastic worker comb foundation allow the assembly of larvae supporting devices or single queen cell bases. The idea was to assemble the hollow plastic worker comb foundation and the larvae supporting device or queen cell bases together to make a complete comb foundation firstly, and then to let the workers build the comb. Next, the queen is allowed to lay eggs in the cells of the comb. This is followed by removing the larvae supporting device or queen cell bases and assembling them into the queen cell bar with regular holes. In this way, royal jelly production or breeding queens can be achieved. This study tested the feasibility of the improved 10th generation of the non-grafting larvae technique of royal jelly harvesting and rearing queens, using the Italian bee (Apis mellifera ligustica) as the experimental system. Firstly, the number of combs in a colony which is about to swarm was reduced, and then the workers were allowed to build 10-12 pieces of combs based on the hollow plastic comb foundation, then the effect of royal jelly harvesting or rearing queens without larvae grafting was investigated. The experiment of royal jelly harvesting without artificially grafting larvae mainly measured the oviposition rate of one queen, two queens, multi-queen (four queens). The acceptance rate of newly assembled queen cells by nursing bees during royal jelly production was also measured. In the experiment of breeding queens without artificially grafting larvae, a single queen was allowed to lay eggs for 6 h. Then the birth weight and the number of ovarioles of the newly bred queens that developed from the eggs were measured. The methods of rearing queens without and with artificially grafting larvae were compared.【Result】Worker bees can build a complete comb based on the plastic hollow comb foundation, and the queen can lay eggs on the newly built comb. The oviposition rate of a single queen, double queens, multi-queen was 91.24%, 92.45% and 91.29%, respectively. The acceptance rate of newly assembled queen cells during royal jelly harvesting was 91.12%, 92.63% and 90.19%, respectively. There was no significant difference among them (P>0.05). The weight of the newly queens using the method of egg-based rearing queens without artificially grafting larvae and the method of grafting larvae based rearing queens was (256.31±3.75) mg and (243.43±2.05) mg, respectively. The number of ovarioles in unilateral ovary was (163.87±9.40) and (154.77±6.74), respectively. There was a significant difference between the two methods (P<0.05).【Conclusion】This study showed that the improved 10th generation of non-grafting larvae oviposition device can be used in royal jelly production and for rearing queens. It is worth popularizing and applying in the beekeeping industry.

    NEWS
    China Launches the IAS1000 Project
    Scientia Agricultura Sinica. 2018, 51(22):  4395-4396.  doi:10.3864/j.issn.0578-1752.2018.22.017
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