干旱胁迫对蜡质含量不同小麦近等基因系光合特性的影响

doi:10.3864/j.issn.0578-1752.2018.22.003

Abstract

Abstract:

【Objective】 The objective of this paper was to investigate the effects of different drought levels on the photosynthetic characteristics of wheat with different wax contents. 【Method】 A pair of wheat near-isogenic lines (NILs), including high-wax line JM205 and low-wax line JM204, were used as experimental materials to simulate the process of soil moisture change in the field by means of gradual drought stress in the artificial climate chamber. The two wheat near-isogenic lines with different waxy contents were planted in the same pot to ensure the same drought degree. With the prolongation of drought treatment, the relative soil water content decreased gradually. Water potential, photosynthetic gas exchange parameters and fluorescence parameters of wheat flag leaf under different soil water contents were measured. 【Result】 Under low water stress (60%-49% of relative soil water content), there was no significant difference in photosynthetic rate (Pn) between flag leaf of JM205 and JM204, however, the photosynthetic rate of both wax and waxy lines decreased gradually, but the less waxy line JM204 showed a greater decline. Under moderate drought stress (Relative soil water content between 49% and 32%), the high-wax plant had higher water potential and larger stomatal opening than that of the low-wax one, so the CO₂ supply was sufficient and the photosynthetic rate was higher. Under moderate drought stress, the PSII actual photochemical efficiency (ΦPSⅡ) and maximum photochemical efficiency (Fv/Fm) decreased more rapidly in the low-wax line, which indicated that PSII electron transfer inhibition and light inhibition were more serious. The JIP-test analysis found that blocked PSII electron transport was mainly due to Q_A to Q_B receptor-side electron transfer restrictions. In contrast, the high-wax line maintained relatively higher photosynthetic capacity under drought conditions, with a smoother electron transfer and less excess light energy, resulting in less light suppression. When relative soil water content dropped below 32%, the water potential and photosynthetic capacity of both NILs decreased significantly and there was no significant difference between them. 【Conclusion】 This study preliminarily improved understanding of the physiological mechanism of leaf wax improving drought tolerance of plants, and the high-wax line JM205 has significant drought resistance in the soil moisture range of 49%-32%, providing theoretical basis for selection and cultivation of drought-resistant wheat varieties.

Key words: common wheat (Triticumaestivum L), nearly isogenetic lines, drought stress, wax, photosynthetic characteristics

YANG YanHui,MA Xiao,ZHANG ZiShan,GUO Jun,LI YueNan,LIANG Ying,SONG JianMin,ZHAO ShiJie. Effects of Drought Stress on Photosynthetic Characteristics of Wheat Near-Isogenic Lines with Different Wax Contents[J].Scientia Agricultura Sinica, 2018, 51(22): 4241-4251.

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References 44

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