大豆抗豆卷叶螟的转录组和蛋白质组关联分析

doi:10.3864/j.issn.0578-1752.2018.07.003

Abstract

Abstract: 【Objective】In order to lay a solid basis for a holistic understanding of the defense molecular mechanism of soybean resistance to Lamprosema indicata in soybean, the correlation analysis of the transcriptomic and proteomic of soybean leaves (the highly resistant line (Gantai-2-2) and the highly susceptible line (Wan 82-178)) under Lamprosema indicate larva feeding for 0 h and 48 h was conducted. 【Method】 In this study, soybeans with high resistance and susceptibility to Lamprosema indicata were selected as the research objects. The gene and protein expression abundance was analyzed for the soybean following the Lamprosema indicata feedings using RNA-Seq and iTRAQ technology, respectively. Then the correlation value between the protein level and the transcription level was calculated.【Result】There were 236 DEPs and 1 064 DEGs, 250 DEPs and 680 DEGs, 213 DEPs and 605 DEGs, 211 DEPs and 468 DEGs identified in HR48/HR0, HS48/HS0, HR0/HS0 and HR48/HS48. The results indicated that the correlations between the quantitative protein and mRNA were 0.156, 0.2687, 0.1149, and 0.035 in HR48/HR0, HS48/HS0, HR0/HS0 and HR48/HS48, and 11, 9, 3 and 4 DEPs/DEGs were identified, of which 11, 9, 2 and 3 with same expression trend, respectively. These differential expression proteins were revealed to be involved in metabolic pathways, ribosome, flavonoid biosynthesis, linoleic acid metabolism, amino sugar and nucleotide sugar metabolism, aminoacyl-RNA biosynthesis, alpha-linoleic acid metabolism, biosynthesis of secondary metabolism, phenylpropanoid biosynthesis, RNA transport, glutathione metabolism, ascorbate andaldarate metabolism. The function correlation analysis results showed that 27 pathways correlated to 101 DEPs and 23 DEGs were identified in HR48/HR0, 15 pathways correlated to 147 DEPs and 16 DEGs were identified in HS48/HS0, 18 pathways correlated to 82 DEPs and 10 DEGs were identified in HR0/HS0, and 1 pathway correlated to 71 DEPs and 2 DEGs were identified in HR48/HS48. Candidate differential expression proteins closely related to insect resistance, such as trypsin inhibitor A-like, kunitz-type trypsin inhibitor, chalcone isomerase 4-like, lipoxygenase-9, alpha-dioxygenase 1-like, linoleate 9S-lipoxygenase 1-like isoform 1, lectin precursor, peroxidase 12-like, stress-induced protein SAM22, scorbate peroxidase 1, cytosolic, and so on. Finally, qRT-PCR analysis confirmed that the two kinds of “omics” results were reliable. 【Conclusion】 In this study, some proteins and metabolic pathways were related to insect-resistant, these differential proteins were directly or indirectly involved in soybean response to Lamprosema indicata.

Key words: soybean, Lamprosema indicata, transcriptomic, proteomic, correlation analysis

ZENG WeiYing, SUN ZuDong, LAI ZhenGuang, CAI ZhaoYan, CHEN HuaiZhu, YANG ShouZhen, TANG XiangMin. Correlation Analysis on Transcriptomic and Proteome of Soybean Resistance to Bean Pyralid(Lamprosema indicata)[J].Scientia Agricultura Sinica, 2018, 51(7): 1244-1260.

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Correlation Analysis on Transcriptomic and Proteome of Soybean Resistance to Bean Pyralid(Lamprosema indicata)

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