Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (17): 3267-3277.doi: 10.3864/j.issn.0578-1752.2022.17.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effects of Seedlings Enriched with Zinc on Cadmium Accumulations and Related Transporter Genes Expressions in Different Rice Cultivars

JIANG XiaoTing1(),HUANG GaoXiang1,2(),XIONG XiaoYing1,HUANG YunPei1,DING ChangFeng2,DING MingJun1,WANG Peng1   

  1. 1School of Geography and Environment, Jiangxi Normal University/Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Nanchang 330022
    2Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008
  • Received:2022-04-07 Accepted:2022-05-22 Online:2022-09-01 Published:2022-09-07
  • Contact: GaoXiang HUANG E-mail:2414461377@qq.com;gxhuang@jxnu.edu.cn

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Abstract:

【Objective】 Rice seedlings enriched with zinc (Zn) is a new method to antagonize cadmium (Cd) within rice, studying the various effects and underlying mechanisms among different rice cultivars is useful to provide a basis for the application of this method. 【Method】 Hydroponic and pot experiments were conducted to test the effects of the Zn enrichment in four cultivars including the Wuyunjing21, Zhejingyou1578, Huanghuazhan, and Huiliangyousimiao. Dynamics of Zn accumulation in different cultivars, and variations of Cd uptake and transport in rice tissues were investigated. Furthermore, the expressions of the Cd-related transporter genes of the root were analyzed to explore the underlying mechanisms responsible for the various effects in different cultivars. 【Result】 Results showed that the Zn cultivation didn’t affect the growth of all rice seedlings. Zn accumulations varied obviously among different cultivars, the Zhejingyou1578 had the highest Zn level (230 μg Zn per plant), followed by the Wuyunjing21 (124 μg Zn per plant), the Huanghuazhan and Huiliangyousimiao had the low levels (85.1-95.0 μg Zn per plant). Zn enrichment greatly influenced the expression of Cd-related transporters in the Zhejingyou1578, the OsZIP7 was down-regulated by 48.7% and OsZIP1 was up-regulated by 81.3%. The expressions of the OsIRT1 and OsZIP7 in the Wuyunjing21 were also down-regulated by 35.9% and 35.0%, respectively, and the OsZIP1 was up-regulated by 31.1%. However, the Cd-related transporters in the Huanghuazhan and Huiliangyousimiao were insensitive to Zn enrichment. As a result, Zn enrichment significantly reduced Cd concentrations in the root and shoot by 26.7% and 36.7% of the Wuyunjing21, and 32.0% and 40.0% of the Zhejingyou1578 with a 12.0% inhibition on the Cd transport, respectively. Furthermore, brown rice Cd were reduced by 37.5%, 36.7% and 25.3% in the Wuyunjing21, Zhejingyou1578, and Huanghuazhan, respectively, while no difference occurred in the Huiliangyousimiao, revealing various effects on reducing Cd among different cultivars induced by the Zn enrichment. Correlation analysis showed that the brown rice Cd was negatively correlated with the shoot Cd which was negatively correlated with the root and shoot Zn, revealing a significant antagonism between Zn and Cd within rice. However, the root and shoot Zn were negatively correlated with the OsZIP7 and OsIRT1 and positively correlated with the OsZIP1 expression, indicating that the Zn enrichment within seedlings influenced the brown rice Cd mainly through regulating the expressions of the OsZIP1, OsZIP7 and OsIRT1. 【Conclusion】 The regulations on Cd-related transporters through Zn enrichment were varied among different cultivars, and the Zhejingyou1578 and Wuyunjing21 were more sensitive, so that the reductions of brown rice Cd were higher than other cultivars.

Key words: soil Cd contamination, antagonistic effect, transporter gene, brown rice, correlation analysis

Table 1

Physical-chemical properties of soils"

pH 有机质
Organic matter (g·kg-1)		 阳离子交换量
Cation exchange capacity (cmol·kg-1)		 黏粒含量
Clay content (%)		 土壤Cd含量
Soil Cd (mg·kg-1)		 有效Cd含量
Available Cd (mg·kg-1)
4.81±0.04 34.50±3.14 8.03±0.42 14.20±1.03 0.68±0.03 0.21±0.02

Fig. 1

Dynamic changes of dry weight of four rice seedlings during the process of Zn cultivation Variance analysis was used to test the difference among different treatments at the same time, and different letters indicated significant differences at P<0.05 level. The same as below"

Fig. 2

Dynamic changes of the total Zn accumulation in four rice seedlings during the process Zn cultivation"

Fig. 3

Relative expressions of Zn and Cd related transporter genes in the roots of four cultivars after enriching rice seedlings with Zn"

Fig. 4

Concentrations of Cd in rice roots (A), shoots (B) and Cd transport factors (C) at the tillering stage"

Fig. 5

Concentrations of Cd in brown rice of different rice cultivars at the mature stage"

Fig. 6

Heatmap of the correlation coefficients among the key factors including the Cd and Zn levels in rice tissues and the related transporter genes * and ** indicate significant differences at the levels of P<0.05 and P<0.01, respectively"

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