不同铵硝配比条件下BPDS-Fe(Ⅱ)对玉米幼苗耐低铁胁迫差异的影响

doi:10.3864/j.issn.0578-1752.2017.07.005

Abstract

Abstract: 【Objective】Iron deficiency in crop production is a very common problem in both developing and developed regions. And screening of Fe-efficient genotypes has become more and more meaningful and promising. In this study, two maize inbred lines Wu312 and Ye478 were used to investigate the effects of BPDS-Fe(Ⅱ) on the phenotypes and physiological traits in maize. This work aimed at unraveling the difference in tolerance to iron deficiency between these two maize inbred lines and their mechanisms. 【Method】BPDS (4,7-diphenyl-l,10-phenanthrolinedisulfonic acid) was used to chelate ferrous iron supplied with two different NH₄⁺-N/NO₃^--N ratios. Phenotypes were observed and some physiological traits were measured, including SPAD value of young leaves, dry matter weights of shoot and root, active Fe concentration in young leaves, and Fe, Mn, Cu, Zn concentrations in shoots. SPAD value was determined by SPAD-502 plus. Samples were cut into pieces and digested by HNO₃-H₂O₂. Trace elements were determined by ICP-AES. Leaf samples of 2.00 g were soaked in 20 mL HCl (mol·L^-1) and determined by ICP-AES.【Result】Root exudation was generated in two inbred lines and the solution pH decreased from 6.0 to approximately 4.0 during the last 24 hours when supplied with NH₄NO₃. Compared with the treatment without iron, shoot dry weights of Wu312 and Ye478 in the treatment of 40 μmol·L^-1 BPDS-Fe(Ⅱ) were decreased by 31% and 64%, respectively; root dry weight of Wu312 showed no significant difference; and root dry weight of Ye478 was declined by 63%. The ratios of R/S of both inbred lines were out of range (normal value, 0.20-0.28). Solution pH has decreased by no more than one unit and plants turned to become healthy when supplied with 0.5 mmol·L^-1 NH₄⁺-N and 3.5 mmol·L^-1 NO₃^--N. Ye478 has the ability to sustain more dry weights in shoots and roots, higher leaf SPAD value as well as active Fe concentration than Wu312, showing remarkable advantages of growth and staying green. When supplied with 0.1 μmol·L^-1 BPDS-Fe(Ⅱ), Wu312 could not compete with Ye478 to produce its fifth leaf, finally turned out to be dead. However, Ye478 produced the fifth leaf and turned to be green obviously. There was no significant difference in shoot Fe concentrations between these two lines. Shoot Fe content was all negatively correlated to Mn, Cu and Zn concentrations in shoots. 【Conclusion】Reduction of the ratio of NH₄⁺-N has improved the solution pH and inhibited root exudation. Ye478 is a Fe-efficient genotype and Wu312 is a Fe-inefficient genotype. The most significant difference of phenotypes between the two inbred lines occurred with 0.1 μmol·L^-1 BPDS-Fe(Ⅱ). Dry matter weights of shoot and root, together with leaf SPAD value were identified to be most suitable physiological traits charactering the tolerance to iron deficiency of maize seedlings. R/S ratios and active Fe concentration were found to be good indicators. Possible mechanisms contained the release of protons, preferential allocation of biomass to roots, and the translocation and redistribution of iron.

Key words: maize, BPDS, ferrous iron, tolerance to iron deficiency, ammonium/nitrate ratios

XU JianQin, CHEN XuLei, YU FuTong. Effects of BPDS-Fe(Ⅱ) on the Difference in Tolerance to Iron Deficiency of Maize Seedlings Under Different Ammonium/Nitrate Ratios[J].Scientia Agricultura Sinica, 2017, 50(7): 1223-1233.

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Effects of BPDS-Fe(Ⅱ) on the Difference in Tolerance to Iron Deficiency of Maize Seedlings Under Different Ammonium/Nitrate Ratios

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