球孢白僵菌对葱蝇成虫血淋巴蛋白质及游离氨基酸的影响

doi:10.3864/j.issn.0578-1752.2017.03.018

Abstract

Abstract: 【Objective】Beauveria bassiana is an important biological agent for control of Delia antiqua. The objective of this study is to assess the changes of proteins and free amino acid in haemolymph of D. antiqua adult infected by B. bassiana at different times, understand the relationship between the contents of proteins and free amino acids and the infection of B. bassiana.【Method】Based on the result of dose-response bioassay against D. antiqua adults infected by B. bassiana,individual newly enclosed D. antiqua adults were inoculated with 5 µL 1×10⁸conidia/mL conidial suspension of the GZGY-1-3 strain by micro-drop method. Then the contents of proteins and free amino acid of at different times (12, 24, 36, 48, 60 and 72 h) were measured in infected and uninfected D. antiqua adult using BCA and HPLC-MS/MS. Finally, the relationship between the infection and the contents of the key free amino acid was analyzed. 【Result】The content of proteins declined first and then increased at 12-36 h. The treatment group and control group showed similar changes. However the contents of proteins of the treatment group were higher than the control group. The content of proteins increased to the maximum in 24 h after inoculation, was 1.20 times greater than the control group. The content of proteins of the treatment group declined to the minimum at 48 h, was only 0.83 lower times than the control group, and increased at 60-72 h. The results of amino acids analysis showed that there were no different of free amino acid between the treatment and control groups of D. antiqua adult. The content of free amino acids decreased in 12-36 h, increased in 48-60 h and decreased in 72 h again after inoculation. The content of free amino acids declined to the minimum in 36 h after inoculation, was 0.63 times lower than the control group and increased to the maximum in 60 h after inoculation, was 2.01 times greater than the control group. After infection, there was no change in the type of free amino acids in the hamolymph, free amino acid content of various fluctuations was quite different. The infection of B. bassiana had significant influence on the contents of important amino acids in the haemolymph. 【Conclusion】 The B. bassiana invaded into the insect body affected the balance of the proteins and amino acids in the haemolymph. It seriously hampered the normal physiological activity of the D. antiqua adult. The results of the experiment indicated that B. bassiana strains couldeffectively control D. antiqua adult.

Key words: Delia antique, Beauveria bassiana, haemolymph, protein, free amino acid

ZHANG Hui, WU ShengYong, WANG XiaoQing, LEI ZhongRen. Changes in the Contents of Proteins and Free Amino Acid in haemolymph of Delia antique Adult Infected by Beauveria bassiana

[J].Scientia Agricultura Sinica, 2017, 50(3): 591-598.

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Changes in the Contents of Proteins and Free Amino Acid in haemolymph of Delia antique Adult Infected by Beauveria bassiana

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