Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (20): 3886-3895.doi: 10.3864/j.issn.0578-1752.2016.20.003

• TILLAGE & CULTIVATION·PHYSIOLOGY & ECOLOGY • Previous Articles     Next Articles

Enzyme Activities and Soil Nutrient Status Associated with Different Aggregate Fractions of Paddy Soils Fertilized with Returning Straw for 24 Years

LI Wei-tao1,2, LI Zhong-pei1,2, LIU Ming1, JIANG Chun-yu1, WU Meng1, CHEN Xiao-fen1,2   

  1. 1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008
    2University of Chinese Academy of Sciences, Beijing 100049
  • Received:2016-04-08 Online:2016-10-16 Published:2016-10-16

Abstract: 【Objective】Paddy soils in subtropical China derived from Quaternary red clay are generally deficient in available nutrients, and fertilizers have long been identified as dominant contributors to increase of crop production. Therefore, a long-term experiment was set up in a paddy field that used to be a wasteland earlier to study the distribution of nutrients and enzyme activities in water stable aggregates under long term application of fertilizers, and the findings will provide a sound basis for rational application of fertilizers to ensure sustainable crop production. 【Method】Soil samples were collected from a 24-year long-term field experiment, which was established in 1990 in the Yingtan Red Soil Ecological Experiment Station. The experiment included four treatments: CK (without fertilization), SM (straw application plus manure), NSM (straw application plus manure and nitrogen fertilizer), NPKSM (straw application plus manure, nitrogen, phosphorus and potassium fertilizers). Undisturbed bulk soils were separated into five aggregate-size classes (>2 mm, 1-2 mm, 0.25-1 mm, 0.053-0.25 mm and <0.053 mm) by wet sieving. Three soil enzymes, invertase, urease, and phosphatase, in water-stable aggregates (WSA) and total nitrogen (TN), available phosphorus (AP), and soil organic C (SOC) were determined. 【Result】Application of fertilizers to infertile paddy soil significantly increased the percentage of macro aggregates (larger than 0.25 mm), decreased the percentage of micro aggregates (smaller than 0.25 mm), and increased the mean weight diameter (MWD) of WSA, which could improve soil structure. The combined application of straw, manure and inorganic fertilizer significantly increased the soil enzyme activities in each size fraction. NSM treatment had the largest impact on invertase and urease activities. NPKSM treatment had the most significant impact on acid phosphatase activity. Compared with the control, NSM treatment increased invertase activity in five size fractions by 20.3%-396.2%; urease increased by 58.6%-372.1%. In NPKSM treatment, acid phosphatase activity in five size fractions increased by 48.9%-94.5%. Compared with the control, SOC in each size fraction of NSM treatment increased by 31.6%-65.1%. Total N increased by 19.8%-51.9%. In NPKSM treatment, available phosphorus content in each of the size fractions increased by 7.4-10 times. Aggregated boosted trees (ABT) analysis showed that the relative influence of SOC on invertase was the largest, accounting for 40.6% of the variation; the composition of soil particle had the largest relative influence on the activity of urease, accounting for 44.9% of the variation. Soil AP had the largest contribution of 41% to the variation in the activity of acid phosphatase. Nonmetric multidimensional scaling (NMDS) analysis indicated that soil aggregates within NPKSM treatment differed from those in CK, SM and NSM treatments. However, SM and NSM treatments showed similar effect on soil fertility. 【Conclusion】The combined application of straw, manure, and inorganic fertilizer significantly increased the mean weight diameter (MWD) of WSA, the contents of SOC, TN, AP, and soil enzyme activities, which improved the soil structure and biological functions.

Key words: paddy soil, straw return, water-stable aggregates, soil nutrients, soil enzymes

[1]    杨宾娟, 黄国勤, 钱海燕. 秸秆还田配施化肥对土壤温度、根际微生物及酶活性的影响. 土壤学报, 2014, 51(1): 150-157.
Yang B J, Huang G Q, Qian H Y. Effects of straw incorporation plus chemical fertilizer on soil temperature, root micro-organisms and enzyme activities. Acta Pedological Sinica, 2014, 51(1): 150-157. (in Chinese)
[2]    黄昌勇. 土壤学. 北京: 中国农业科技出版社, 2000: 199-201.
Huang C Y. Soil Science. Beijing: Chinese Agricultural Science and Technology Press, 2000: 199-201. (in Chinese)
[3]    Tisdall J M. Possible role of soil microorganisms in aggregation in soils. Plant Soil, 1994, 159: 115-121.
[4]    Denef K, Six J. Clay mineralogy determines the importance of biological versus abiotic processes for macroaggregate formation and stabilization. European Journal of Soil Science, 2005, 56(4): 469-479.
[5]    周礼恺. 土壤酶学. 北京: 科学出版社, 1987.
Zhou L K. Soil Enzymology. Beijing: Science Press, 1987. (in Chinese)
[6]    聂军, 郑圣先, 杨曾平, 廖育林, 谢坚. 长期施用化肥、猪粪和稻草对红壤性水稻土物理性质的影响. 中国农业科学, 2010, 43(7): 1404-1413.
NIE J, ZHENG S X, YANG Z P, LIAO Y L, XIE J. Effects of long-term application of chemical fertilizer, pig manure and rice straw on physical properties of a reddish paddy soil. Scientia Agricultura Sinica, 2010, 43(7): 1404-1413. (in Chinese)
[7]    Wang R Z, Dorodnikov M, Yang S, Zhang Y Y, Filley T R, Turco R F, Zhang Y G, Xu Z W, Li H, Jiang Y. Responses of enzymatic activities within soil aggregates to 9-year nitrogen and water addition in a semi-arid grassland. Soil Biology and Biochemistry, 2015, 81: 159-167.
[8]    劳秀荣, 孙伟红, 王真, 郝艳茹, 张昌爱. 秸秆还田与化肥配合施用对土壤肥力的影响. 土壤学报, 2003, 40(4): 618-623.
Lao X R, Sun W H, Wang Z, Hao Y R, Zhang C A. Effect of matching use of straw and chemical fertilizer on soil fertility. Acta Pedological Sinica, 2003, 40(4): 618-623. (in Chinese)
[9]    Wang W, Chen W C, Wang K R, Xie X L, Yin C M, Chen A L. Effects of long-term fertilization on the distribution of carbon, nitrogen and phosphorus in water-stable aggregates in paddy soil. Agricultural Sciences in China, 2011, 10(12): 1932-1940.
[10]   Zhang Q, Zhou W, Liang G Q, Sun J W, Wang X B, He P. Distribution of soil nutrients, extracellular enzyme activities and microbial communities across particle-size fractions in a long-term fertilizer experiment. Applied Soil Ecology, 2015, 94: 59-71.
[11]   陈晓芬, 李忠佩, 刘明, 江春玉. 不同施肥处理对红壤水稻土团聚体有机碳、氮分布和微生物生物量的影响. 中国农业科学, 2013, 46(5): 950-960.
Chen X F, Li Z P, Liu M, Jiang C Y. Effects of different fertilizations on organic carbon and nitrogen contents in water-stable aggregates and microbial biomass content in paddy soil of subtropical China. Scientia Agricultura Sinica, 2013, 46(5): 950-960. (in Chinese)
[12]   Tian K, Zhao Y C, Xu X H, Hai N, Huang B A, Deng W J. Effects of long-term fertilization and residue management on soil organic carbon changes in paddy soils of China: A meta-analysis. Agriculture, Ecosystems and Environment, 2015, 204: 40-50.
[13]   谢正苗, 吕军, 俞正炎, 黄昌勇. 红壤退化过程与生态位研究. 应用生态学报, 1998, 9(6): 669-672.
Xie Z M, Lü J, Yu Z Y, Huang C Y. Degradation process of red soil and its niche. Chinese Journal of Applied Ecology, 1998, 9(6): 669-672. (in Chinese)
[14]   中国科学院南京土壤研究所. 中国土壤. 北京: 科学出版社, 1978: 50-53.
Institute of Soil Science, Chinese Academy of Science. Chinese Soil. Beijing: Science Press, 1978: 50-53. (in Chinese)
[15]   李忠佩, 唐永良, 石华, 高坤林. 不同施肥制度下红壤稻田的养分循环与平衡规律. 中国农业科学, 1998, 31(1): 46-54.
Li Z P, Tang Y L, Shi H, Gao K L. Nutrient cycling and balance of paddy fields in different fertilization systems in red soil region of subtropical China. Scientia Agricultura Sinica, 1998, 31(1): 46-54. (in Chinese)
[16]   Elliott E T. Aggregate structure and carbon, nitrogen and phosphorus in native and cultivated soils. Soil Science Society of America Journal, 1986, 50(3): 627-633.
[17]   关松荫. 土壤酶学研究方法. 北京: 中国农业科技出版社, 1986: 274-314.
Guan S Y. Methods of soil enzymology Chinese) . Beijing: Chinese Agricultural Science and Technology Press, 1986: 274-314. (in
[18]   鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 1999.
Lu R K. Analytical Methods of Soil Agricultural Chemistry. Beijing: Chinese Agricultural Science and Technology Press, 1999. (in Chinese)
[19]   De'ath G. Boosted trees for ecological modeling and prediction. Ecology, 2007, 88: 243-251.
[20]   陈春梅, 谢祖彬, 朱建国. 土壤有机碳激发效应研究进展. 土壤, 2006, 38(4): 359-365.
Chen C M, Xie Z B, Zhu J G. Advances in research on priming effect of soil organic carbon. Soils, 2006, 38(4): 359-365.
[21]   Su J Q, Ding L J, Xue K, Yao H Y, Quensen J, Bai S J, Wei W X, Wu J S, Zhou J Z, Tiedje J M, Zhu Y G. Long-term balanced fertilization increases the soil microbial functional diversity in a phosphorus-limited paddy soil. Molecular Ecology, 2015, 24: 136-150.
[22]   Liu Y R, Li X, Shen Q R, Xu Y C. Enzyme activity in water-stable soil aggregates as affected by long-term application of organic manure and chemical fertiliser. Pedosphere, 2013, 23(1): 111-119.
[23]   Tyler S W, Wheatcraft S W. Fractal scaling of soil particle- size distributions-analysis and limitations. Soil Science Society of America Journal, 1992, 56(2): 362-369.
[24]   Lagomarsino A, Grego S, Kandeler E. Soil organic carbon distribution drives microbial activity and functional diversity in particle and aggregate-size fractions. Pedobiologia, 2012, 55(2), 101-110.
[25]   Oades J M. Soil organic-matter and structural stability - mechanisms and implications for management. Plant and Soil, 1984, 76(1/3): 319-337.
[26]   Bach E M, Hofmockel K S. Soil aggregate isolation method affects measures of intra-aggregate extracellular enzyme activity. Soil Biology and Biochemistry, 2014, 69: 54-62.
[27]   Chen X F, Li Z P, Liu M, Jiang C Y, Che Y P. Microbial community and functional diversity associated with different aggregate fractions of a paddy soil fertilized with organic manure and/or NPK fertilizer for 20 years. Journal of Soils Sediments, 2015, 15: 292-301.
[28]   Burns R G. Soil enzymes. London: Academic Press, 1978: 9-11.
[29]   WANG J, WANG D J, ZHANG G, WANG C. Effect of wheat straw application on ammonia volatilization from urea applied to a paddy field. Nutrient Cycling in Agroecosystems, 2012, 94(1): 73-84.
[30]   Sinsabaugh R L. Enzymatic Analysis of Microbial Pattern and Process. Biology and Fertility of Soils, 1994, 17(1): 69-74.
[31]   Schnecker J, Wild B, Takriti M, Alves R J E, Gentsch N, Gittel A, Hofer A, Klaus K, Knoltsch A, Lashchinskiy N, Mikutta R, Richter A. Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia. Soil Biology and Biochemistry, 2015, 83: 106-115.
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