外源一氧化氮对干旱胁迫下玉米幼苗光合作用的影响

doi:10.3864/j.issn.0578-1752.2016.02.006

Abstract

Abstract: 【Objective】To evaluate the effects of exogenous nitric oxide on the photosynthesis of maize (Zea mays L.) under drought stress. 【Method】The hydroponic experiment was conducted in a growth chamber with the Zhuyu 309 as the material. After being pretreated with sodium nitroprusside (SNP) for three days, these materials were simulated drought stress with 20% PEG-6000 for another three days. The changes of maize seedling’s growth, gas exchange, and chlorophyll fluorescence parameters, chloroplast ultrastructure and expression of D1 protein were investigated; 【Result】The photosynthetic rate (Pn), stomatal conductance (Gs), PSII maximum photochemical efficiency (Fv/Fm), PSII potential activity (Fv/Fo) were decreased by 68.4%, 87.2%, 15.8%, 31.3%，respectively under drought stress; The parameters of relative variable fluorescence intensity at J-step (Vj) and dissipated energy flux per reaction center (DIo/RC) increased by 20.9% and 21.2%. The ultrastructure of chloroplast in the leaf was destroyed and the structure of stacked grana was disintegrated. Moreover, the osmiophilic grains increased and the chloroplast was slowly divorced from the cell wall due to the drought. The content of D1 protein decreased significantly with Western-blotting analysis. However, with the treatment of SNP and then drought stress, the Pn, Gs, Fv/Fm, Fv/Fo were increased by 56.6 %, 202.5 %, 15.8 %, 30.7 % and the Vj decreased by 22.7% and 25.4% compared with the drought stress. The thylakoid lamellae arranged regularly and the grana were clear; and the relative amounts of D1 protein increased by 94.7%. The above changes were related to the increased NO content after SNP pretreatment under drought condition.【Conclusion】These results suggested that NO is involved in regulating the D1 protein expression and stabilizing the structure and function of PSII reaction centers to improve photosynthesis and growth of maize seedlings, which improved the adaptability of maize seedlings to drought stress.

Key words: maize, drought stress, nitric oxide, D1 protein, photosynthesis, chloroplast ultrastructure

SHAO Rui-xin, LI Lei-lei, ZHENG Hui-fang, XIN Long-fei, SU Xiao-yu, RAN Wu-ling, YANG Qing-hua. Effects of Exogenous Nitric Oxide on Photosynthesis of Maize Seedlings Under Drought Stress[J].Scientia Agricultura Sinica, 2016, 49(2): 251-259.

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Effects of Exogenous Nitric Oxide on Photosynthesis of Maize Seedlings Under Drought Stress

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