一个黄麻矮秆种质株高性状对外源激素的敏感性

doi:10.3864/j.issn.0578-1752.2015.10.003

Abstract

Abstract: 【Objective】The aim of this study was to illuminate the sensibility of the exogenous hormones to plant height of a dwarf germplasm Aidianyehuangma so as to determine which kind of hormone sensitivity this germplasm belongs to, which will facilitate dissecting the relationship between the genetic basis of plant height and hormonal regulation in jute. 【Method】 Firstly, the performance of important agricultural traits, e.g. plant height, were compared between Aidianyehuangma and the variety Huangma 179 with normal plant height. Secondly, the dwarf germplasm of Aidianyehuangma was treated with different concentrations of exogenous GA₃, IAA and BR. And the hypocotyl length in the Ms culture media and plant height variation in the field were investigated. Meanwhile, the hypocotyl length of Huangma 179 and Aidianyehuangma on the GA₃ culture media was evaluated. Thirdly, the effects of continuous spraying of 100 mg·L^-1GA₃ every 3 days on plant height of Aidianyehuangma were analyzed. Furthermore, the activities of α - amylase of Aidianyehuangma were analyzed after it was once treated with GA₃. 【Result】 The comparison analysis of important agricultural traits showed that the plant height of Aidianyehuangma (1.89 m) was significantly lower than that of Huangma 179 (4.28 m) without exogenous hormone treatment, indicating that Aidianyehuangma is a typical dwarf germplasm in jute. After being treated with MS culture media of 0.1 mg^.L^-1 exogenous IAA and BR, the hypocotyl length of Aidianyehuangma did not show a significant difference with that of the control. However, after being treated with MS culture media of 0.1 mg·L^-1 exogenous GA₃, the hypocotyl length of Aidianyehuangma showed a significant difference compared with Aidianyehuangma without being treated with GA₃, and the hypocotyl length of Aidianyehuangma and Huangma 179 was similar to that of Huangma 179 without being treated with GA₃. It suggested that the plant height of Aidianyehuangma is sensitive to exogenous GA₃ while that of Huangma 179 is not sensitive to the exogenous GA₃. After being treated with different concentrations of exogenous hormone in the field, plant height of Aidianyehuangma with high concentration (100 mg·L^-1) of GA₃ was significantly higher than that with the other two lower concentrations of GA₃. By contrast, plant height of Aidianyehuangma treated with different concentrations of exogenous IAA and BR did not show a significant difference with that of the control. It further suggested that Aidianyehuangma is a GA₃ sensitive dwarf germplasm. After being treated with 100 mg·L^-1 exogenous GA₃ continuously in the field, the plant height of Aidianyehuangma showed a significant difference in comparison of Aidianyehuangma without being treated with GA₃, reaching a significant level, and appoached that of Huangma 179. It showed that plant height of Aidianyehuangma could be resumed using exogenousGA₃. After Aidianyehuangma was once treated with GA₃, α- amylase activity of Aidianyehuangma in the first 3 days began to increase in comparison with that before treatment, and enhanced significantly in the first 6 days, which indicated that the exogenous GA₃ could increase the α- amylase activity of Aidianyehuangma. 【Conclusion】Aidianyehuangma is a typical dwarf germplasm in jute. The plant height of Aidianyehuangma is not sensitive to exogenous IAA and BR, but sensitive to the exogenous GA₃, and can be resumed to normal plant height with continuous exogenous GA₃ treatment. The exogenous GA₃ could increase the α- amylase activity of Aidianyehuangma. Aidianyehuangma is a GA₃ sensitive dwarf germplasm.

Key words: jute, dwarf, plant height, hormones, GA₃sensitivity

ZHANG Li-wu, HUANG Wen-xuan, TAO Ai-fen, LIN Li-hui, XU Jian-tang, FANG Ping-ping, QI Jian-min. Research of Exogenous Hormone Sensitivity on Plant Height of a Dwarf Germplasm in Jute (Corchorus capsularis L.)[J].Scientia Agricultura Sinica, 2015, 48(10): 1892-1899.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2015.10.003

https://www.chinaagrisci.com/EN/Y2015/V48/I10/1892

References

[1] 熊和平. 麻类作物育种学: 第1版. 北京: 中国农业科学技术出版社, 2008: 156-185.

Xiong H P. Breeding Sciences of Bast and Leaf Fiber Crops: 1st edn. Beijing: China Agricultural Science and Technology Press, 2008: 156-185. (in Chinese)

[2] 祁建民, 卢浩然, 郑云雨, 王英娇. 黄麻数量性状遗传关系分析. 作物学报, 1991, 17(2): 145-150.

Qi J M, Lu H R, Zheng Y Y, Wang Y J. Genetic relationship analysis of quantitative traits in jute. Acta Agronomica Sinica, 1991, 17(2): 145-150. (in Chinese)

[3] 孙家曾, 余隆其, 何广文. 黄麻主要数量性状遗传力和相关性的研究. 中国农业科学, 1981, 14(03): 25-32.

Sun J Z, Yu J Q, He G W. Heritability and correlation studies of main quantitative traits in jute. Scientia Agricultura Sinica, 1981, 14(03): 25-32. (in Chinese)

[4] Peng J, Richards D E, Hartley N M, Murphy G P, Devos K M, Flintham J E, Beales J, Fish L J, Worland A J, Pelica F, Sudhakar D, Christou P, Snape J W, Gale M D, Harberd N P. ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature, 1999, 400: 256-261.

[5] Hedden P. The genes of the Green Revolution. Trends in Genetics, 2003, 19(1): 5-9.

[6] Sun T, Gubler F. Molecular mechanism of gibberellin signaling in plants. Annual Review of Plant Biology, 2004, 55: 197-223.

[7] Suzuki Y, Saso K, Fujioka S, Yoshida S, Nitasaka E, Nagata S, Nagasawa H, Takatsuto S, Yamaguchi I. A dwarf mutant strain of Pharbitis nil, Uzukobito (kobito), has defective brassinosteroid biosynthesis. The Plant Journal, 2003, 36: 401-410.

[8] Sazuka T, Kamiya N, Nishimura T, Ohmae K, Sato Y, Imamura K, Nagato Y, Koshiba T, Nagamura Y, Ashikari M, Kitano H, Matsuoka M. A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. The Plant Journal, 2009, 60: 227-241.

[9] Phinney B O. Gibberellin A₁ dwarfism and shoot elongation in higher plants. Biologia Plantarum, 1985, 27(2/3): 172-179.

[10] Harberd N P, Freeling M. Genetics of dominant gibberellin-insensitive dwarfism in maize. Genetics, 1989, 121: 827-838.

[11] Mitsunaga S, Tashiro T, Yamaguchi J. Identification and characterization of gibberellin-insensitive mutants selected from among dwarf mutants of rice. Theoretical and Applied Genetics, 1994, 87: 705-712.

[12] Koka C V, Cerny R E, Gardner R G, Noguchi T, Fujioka S, Takatsuto S, Yoshida S, Clouse S D. A putative role for the tomato genes DUMPY and CURL-3 in brassinosteroid biosynthesis and response. Plant Physiology, 2000, 122: 85-98.

[13] Yamamuro C, Ihara Y, Wu X, Noguchi T, Fujioka S, Takatsuto S, Ashikari M, Kitano H, Matsuoka M. Loss of function of a rice brassinosteroid insensitive homolog prevents internode elongation and bending of the lamina joint. The Plant Cell, 2000, 12: 1591-1605.

[14] Nomura T, Kitasaka Y, Takatsuto S, Reid J B, Fukami M, Yokota T. Brassinosteroid/sterol synthesis and plant growth as affected by lka and lkb mutations of pea. Plant Physiology, 1999, 119: 1517-1526.

[15] Raventos D, Meier C, Jensen O M B, Mundy J. Fusion genetic analysis of gibberellin signaling mutants. The Plant Journal, 2000, 22(5): 427-438.

[16] Mitsunaga S, Tashiro T, Yamaguchi J. Identification and characterization of gibberellin-insensitive mutants selected from among dwarf mutants of rice. Theoretical and Applied Genetics, 1994, 87: 705-712.

[17] 卢浩然, 郑云雨, 朱秀英, 王英娇. 黄麻七个经济性状遗传力的研究. 中国麻作, 1980, 1: 6-8.

Lu H R, Zheng Y Y, Zhu X Y, Wang Y J. Genetic studies of seven economic traits in jute. China’s Fiber Crops, 1980, 1: 6-8. (in Chinese)

[18] 郭安平, 龚友才. 圆果种黄麻主要农艺性状的遗传相关及通径分析. 中国麻作, 1988, 2: 6-9.

Guo A P, Gong Y C. Genetic correlation and path analysis of the main agronomic traits in white jute, China’s Fiber Crops, 1988, 2: 6-9. (in Chinese)

[19] Green C E, Phillips R L. Plant regeneration from tissue cultures of maize. Crop Science, 1975, 15: 417-421.

[20] 王学奎. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 167-176.

Wang X K. Experiment Theory and Technology on Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2000: 167-176. (in Chinese)

[21] 张超, 孙君灵, 贾银华, 周忠丽, 潘兆娥, 何守朴, 徐正君, 杜雄明. 外源激素对一个新的棉花极端矮化突变体 AS98植株生长和酶活性的影响. 中国农业科学, 2010, 43(7): 1370-1378.

Zhang C, Sun J L, Jia Y H, Zhou Z L, Pan Z E, He S P, Xu Z J, Du X M. Effect of exogenous hormone on plant growth and enzyme activity of a novel cotton dwarf-mutant AS98. Scientia Agricultura Sinica, 2010, 43(7): 1370-1378. (in Chinese)

[22] Wang E, Wang J, Zhu X, Hao W, Wang L, Li Q, Zhang L, He W, Lu B, Lin H, Ma H, Zhang G, He Z. Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nature Genetics, 2008, 40: 1370-1374.

[23] Xu Y H, Zhu Y Y, Zhou H C, Li Q, Sun Z X, Liu Y G, Lin H X, He Z H. Identification of a 98-kb DNA segment containing the rice Eui gene controlling uppermost internode elongation, and construction of a TAC transgene sublibrary. Molecular Genetics and Genomics, 2004, 272: 149-155.

[24] Li S, Li W, Huang B, Cao X, Zhou X, Ye S, Li C, Gao F, Zou T, Xie K, Ren Y, Ai P, Tang Y, Li X, Deng Q, Wang S, Zheng A, Zhu J, Liu H, Wang L, Li P. Natural variation in PTB1 regulates rice seed setting rate by controlling pollen tube growth. Nature Communications, 2013, 4: 2793-2805.

[25] Spielmeyer W, Ellis M H, Chandler P M. Semidwarf (sd-1), “green revolution”rice, contains a defective gibberellin 20-oxidase gene. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99: 9043-9048.

[26] Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, Maehara Y, Tanji M, Sato M, Nasu S, Minobe Y. Positional cloning of rice Semidwarfing Gene, sd-1: Rice ‘green revolution gene’ encodes a mutant enzyme involved in gibberellin synthesis. DNA Research, 2002, 9: 11-17.

Related Articles 15

[1]	LIU Shuo,ZHANG Hui,GAO ZhiYuan,XU JiLi,TIAN Hui. Genetic Variations of Potassium Harvest Index in 437 Wheat Varieties [J]. Scientia Agricultura Sinica, 2022, 55(7): 1284-1300.
[2]	SHA YueXia, HUANG ZeYang, MA Rui. Control Efficacy of Pseudomonas alcaliphila Strain Ej2 Against Rice Blast and Its Effect on Endogenous Hormones in Rice [J]. Scientia Agricultura Sinica, 2022, 55(2): 320-328.
[3]	LI XiaoYing, WU JunKai, WANG HaiJing, LI MengYuan, SHEN YanHong, LIU JianZhen, ZHANG LiBin. Characterization of Volatiles Changes in Chinese Dwarf Cherry Fruit During Its Development [J]. Scientia Agricultura Sinica, 2021, 54(9): 1964-1980.
[4]	GAO ZhiYuan,XU JiLi,LIU Shuo,TIAN Hui,WANG ZhaoHui. Variations of Winter Wheat Nitrogen Harvest Index in Field Wheat Population [J]. Scientia Agricultura Sinica, 2021, 54(3): 583-595.
[5]	GUO ShuQing,SONG Hui,YANG QingHua,GAO JinFeng,GAO XiaoLi,FENG BaiLi,YANG Pu. Analyzing Genetic Effects for Plant Height and Panicle Traits by Means of the Mixed Inheritance Model of Major Gene Plus Polygene in Foxtail Millet [J]. Scientia Agricultura Sinica, 2021, 54(24): 5177-5193.
[6]	CHEN Yang,WANG Lei,BAI YouLu,LU YanLi,NI Lu,WANG YuHong,XU MengZe. Quantitative Relationship Between Effective Accumulated Temperature and Plant Height & Leaf Area Index of Summer Maize Under Different Nitrogen, Phosphorus and Potassium Levels [J]. Scientia Agricultura Sinica, 2021, 54(22): 4761-4777.
[7]	WANG JunJie,TIAN Xiang,QIN HuiBin,WANG HaiGang,CAO XiaoNing,CHEN Ling,LIU SiChen,QIAO ZhiJun. Regulation Effects of Photoperiod on Growth and Leaf Endogenous Hormones in Broomcorn Millet [J]. Scientia Agricultura Sinica, 2021, 54(2): 286-295.
[8]	ZHENG FengSheng,WANG HaiHua,WU QingTao,SHEN Quan,TIAN JianHong,PENG XiXu,TANG XinKe. Genome-Wide Identification of VQ Gene Family in Fagopyrum tataricum and Its Expression Profiles in Response to Leaf Spot Pathogens [J]. Scientia Agricultura Sinica, 2021, 54(19): 4048-4060.
[9]	LI YanLin,SHAHID Iqbal,SHI Ting,SONG Juan,NI ZhaoJun,GAO ZhiHong. Isolation of PmARF17 and Its Regulation Pattern of Endogenous Hormones During Flower Development in Prunus mume [J]. Scientia Agricultura Sinica, 2021, 54(13): 2843-2857.
[10]	LIU HaiYing,FENG BiDe,RU ZhenGang,CHEN XiangDong,HUANG PeiXin,XING ChenTao,PAN YinYin,ZHEN JunQi. Relationship Between Phytohormones and Male Sterility of BNS and BNS366 in Wheat [J]. Scientia Agricultura Sinica, 2021, 54(1): 1-18.
[11]	ZHU XingHao,CHEN Qing,SHAO BingHao,GUO YuJun,ZHANG XiangLi,DU PengFei,ZHU Yao,HUANG YanQun,CHEN Wen. Effect of the Heterozygous Sex-Linked Dwarf Gene on Fat Deposition in Normal Type Chickens [J]. Scientia Agricultura Sinica, 2021, 54(1): 213-223.
[12]	WANG JunJie,WANG HaiGang,CAO XiaoNing,CHEN Ling,LIU SiChen,TIAN Xiang,QIN HuiBin,QIAO ZhiJun. Comprehensive Evaluation of Photoperiod Sensitivity Based on Different Traits of Broomcorn Millet [J]. Scientia Agricultura Sinica, 2020, 53(3): 474-485.
[13]	HAO YanRong,DU Wei,HOU SiYu,WANG DongHang,FENG HongMei,HAN YuanHuai,ZHOU MeiLiang,ZHANG KaiXuan,LIU LongLong,WANG JunZhen,LI HongYing,SUN ZhaoXia. Identification of ARF Gene Family and Expression Pattern Induced by Auxin in Fagopyrum tataricum [J]. Scientia Agricultura Sinica, 2020, 53(23): 4738-4749.
[14]	ZOU JianQiu. New Research Progress on Sorghum Breeding and Cultivation Techniques [J]. Scientia Agricultura Sinica, 2020, 53(14): 2769-2773.
[15]	ZOU JianQiu,WANG YanQiu,LI JinHong,ZHU Kai. Dwarfing Effect and Molecular Mechanism of An Elite Sorghum Male Sterile Line 01-26A in Its Hybrids [J]. Scientia Agricultura Sinica, 2020, 53(14): 2814-2827.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Research of Exogenous Hormone Sensitivity on Plant Height of a Dwarf Germplasm in Jute (Corchorus capsularis L.)

RichHTML

PDF

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0