Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (24): 5081-5088.doi: 10.3864/j.issn.0578-1752.2013.24.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Main, Environmentally Interacted and Epistatic QTL for Seed Shape Traits in Soybean

 LIANG  Hui-Zhen-1, YU  Yong-Liang-1, YANG  Hong-Qi-1, ZHANG  Hai-Yang-1, DONG  Wei-1, DU  Hua-1, CUI  Wei-Wen-1, LIU  Xue-Yi-2, FANG  Xuan-Jun-3   

  1. 1.Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002;
    2.Economical Crops Institute, Shanxi Academy of Agricultural Sciences, Fenyang 032200, Shanxi;
    3.Hainan Institute of Tropical Agriculture Resources, Sanya 572025, Hainan
  • Received:2013-07-24 Online:2013-12-16 Published:2013-09-30

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Abstract: 【Objective】In this study, the mixed linear model method was used to identify the main-effect, environmentally interacted and epistatic quantitative trait loci (QTLs) for seed shape traits in soybean.【Method】A total of 447 recombinant inbred lines (RILs) derived from a cross of Jindou 23 (cultivar, female parent) and ZDD2315 (semi-wild, male parent) were scanned by 232 SSR markers and measured for the above traits in 2010 to 2012. The marker information was used to construct linkage groups. All the phenotypic values along with marker and linkage-group information were used to detect all kinds of QTLs for the above traits.【Result】 Seven QTLs for seed length, seed width, seed thickness, seed length-to-width ratio, seed length-to-thickness ratio and seed width-to-thickness ratio, were mapped and placed on linkage groups D2, C2, J_2 and O. Positive additive effects of QTLs for seed length, seed length-to-thickness ratio and seed width-to-thickness ratio were observed and their elite alleles were derived from Jindou 23. Three pairs of additive × additive epistasis and their interactions with environment for seed width, seed width-to-thickness ratio were detected.【Conclusion】The main-effect, epistatic and environmentally interacted QTLs have the biggest, middle and smallest influences on the above traits, respectively.

Key words: soybean , seed shape trait , QTL and environment interaction , epistasis , mixed linear model

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