Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (22): 4646-4656.doi: 10.3864/j.issn.0578-1752.2013.22.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Soybean Maturity SSSLs and QTL Mapping Based on Meta-Analysis

 CHE  Jing-Yu-12, LIU  Chun-Yan-3, JIANG  Hong-Wei-13, HAN  Xue-3, MAO  Yan-Zhi-12, XIN  Da-Wei-1, CHEN  Qing-Shan-1, HU  Guo-Hua-3   

  1. 1.Agriculture College, Northeast Agriculture University, Harbin 150030;
    2.Keshan Branch of Heilongjiang Academy of Agriculture Science, Keshan 161606, Heilongjiang
    3.Crop Research and Breeding Center of Land-Reclamation, Harbin 150090
  • Received:2013-05-26 Online:2013-11-15 Published:2013-09-12

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Abstract: 【Objective】Soybean maturity is a quantitative trait controlled by multiple genes, which is a key trait affecting soybean yield and adaptability. The heredity of soybean maturity with single segment substitution lines (SSSLs) was studied and the main effect QTLs of soybean maturity were identified. 【Method】 Fifteen sets of backcross introgression lines were constructed by using Hongfeng 11 as recurrent parent and 15 soybean varieties as the donor parent, respectively. Based on the results of meta-analysis of soybean maturity (R8), the SSR markers from real QTL interval were used to identify SSSLs. Using the method of figure genotype calculated the length of substitution fragment, using the statistical software SPSS analyzed the main effect QTLs of soybean maturity. 【Result】Sixteen substitution fragments were detected on linkage group C2 and L. Among them, 7 substitution fragments were detected on linkage group C2, substitution fragment length was 9.8cM in total. Nine substitution fragments were detected on linkage group L, and the substitution fragment length was 37.212 cM in total. Previous researches show that Sat_010 and Satt156 are the specific markers for E4/e4 on linkage group L, and 5 QTLs（Sat_238, Satt460, Sct_010, Satt166, and Sat_113 ） were identified on the maturity in one-way. Single fragment Satt460 could shorten the growth period of soybean, and single fragments Sat_238, Sct_010 and Sat_113 could delay soybean growth stages. Sat_238 Satt460, Sct_010, Satt166, and Sat_113 were identified to be the important SSR markers on soybean maturity. 【Conclusion】Five markers from meta-analysis were identified as the important markers on soybean maturity. The single fragment Satt460 could shorten the soybean growth period, single segments Sat_238, Sct_010, and Sat_113 could delay soybean growth period.

Key words: soybean , maturity , meta-analysis , single segment substitution lines (SSSLs) , SSR

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