Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4197-4209.doi: 10.3864/j.issn.0578-1752.2013.20.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity Research of Mungbean Germplasm Resources by SSR Markers in China

 LIU  Yan, CHENG  Xu-Zhen, WANG  Li-Xia, WANG  Su-Hua, BAI  Peng, WU  Chuan-Shu   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2013-04-10 Online:2013-10-15 Published:2013-07-08

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Abstract: 【Objective】The purpose of this research was to investigate the genetic diversity, relationship and differentiation of cultivated mungbean germplasm resources in China, and provide useful information on effective utilization of resources, new genes mining and breeding.【Method】The genetic diversity of 18 mungbean groups from different areas was analyzed by using 40 SSR markers.【Result】A total of 125 alleles were identified with an average of 3.1 alleles per locus. The average of effective number of alleles (NE), Nei's gene diversity (H), polymorphism information content(PIC), expected heterozygosity (He), and Shannon’s information index (I) were 1.8, 0.4233, 0.3497, 0.4241, and 0.6754, respectively. Hebei, Shandong and Anhui provinces had a higher genetic variation than other provinces. The average observed heterozygosity (Ho) was 0.1001 and the average of inbreeding coefficient among population(Fis) was 0.6759, indicating that a certain degree of inbreeding existed in Chinese mungbean germplasm. The gene flow (Nm= 0.6936) and the average genetic differentiation (Fst= 0.2649) also showed a high genetic variation within Chinese mungbean. A total of 272 individuals were divided into 2 groups and 18 populations were divided into 3 groups based on Popgene software. The genetic distances between groups were agreeable with their geographical origin.【Conclusion】There was a high genetic diversity level within Chinese mungbean germplasm. Geographical environment and ecological factors affect the genetic differentiation among different populations.

Key words: mungbean , SSR , genetic diversity , genetic relationship

[1]郑卓杰. 中国食用豆类学. 北京: 中国农业出版社, 1997: 141-166.

Zheng Z J. Food Legumes in China. Beijing: Chinese Agricultural Press, 1997: 141-166. (in Chinese)

[2]王丽侠, 程须珍, 王素华. 绿豆种质资源、育种及遗传研究进展. 中国农业科学, 2009, 42(5): 1519-1527.

Wang L X, Cheng X Z, Wang S H. Advances in research on genetic resources, breeding and genetics of mungbean (Vigna radiata L.). Scientia Agricultura Sinica, 2009, 42(5): 1519-1527. (in Chinese)

[3]程须珍, 王素华. 中国绿豆品种资源研究. 作物品种资源, 1998, 4: 9-11.

Cheng X Z, Wang S H. Research of mungbeanvariety resourcesin China. Crop Varietyresource, 1998, 4: 9-11. (in Chinese)

[4]韩粉霞, 李桂英. 绿豆主要农艺性状的相关分析. 华北农学报, 1998, 13(4): 66-69.

Han F X, Li G Y. Mungbean correlation analysis of main agronomic traits. Acta Agriculturae Boreali Sinica, 1998, 13(4): 66-69. (in Chinese)

[5]刘长友, 程须珍, 王素华, 王丽侠, 孙蕾, 梅丽, 徐宁. 中国绿豆种质资源遗传多样性研究. 植物遗传资源学报, 2006, 7(4): 459-463.

Liu C Y, Cheng X Z, Wang S H, Wang L X, Sun L, Mei L, Xu N. The genetic diversity of mungbean germplasm in China. Journal of Plant Genetic Resources, 2006, 7(4): 459-463. (in Chinese)

[6]王丽侠, 程须珍, 王素华, 李金榜, 李金秀. 中国绿豆应用型核心样本农艺性状的分析. 植物遗传资源学报, 2009, 10(4): 589-593.

Wang L X, Cheng X Z, Wang S H, Li J B, Li J X. Genetic variability of agronomic traits in mungbean applied core collection of China. Journal of Plant Genetic Resources, 2009, 10(4): 589-593. (in Chinese)

[7]王成, 闺锋, 崔秀辉, 李清泉, 曾玲玲, 刘峰. 绿豆农艺性状的遗传多样性分析. 杂粮作物, 2010, 30(3): 182-184.

Wang C, Gui F, Cui X H, Li Q Q, Zeng L L, Liu F. Genetic diversity analysis of mungbean agronomic traits. Rain Fed Crops, 2010, 30(3): 182-184. (in Chinese)

[8]张毅华, 张耀文, 张泽燕. 绿豆种质资源表型性状多样性分析. 农学学报, 2013, 3(1): 15-19.

Zhang Y H, Zhang Y W, Zhang Z Y. Phenotypic diversity analysis of mungbean germplasm resources. Journal of Agriculture, 2013, 3(1): 15-19. (in Chinese)

[9]程须珍, 杨又迪. RAPD分析在绿豆亲缘关系研究中的应用. 遗传, 1998, 20(增刊): 27-29.

Cheng X Z, Yang Y D. Study of mungbean genetic relationship using RAPD markers. Heredita, 1998, 20(Suppl.): 27-29. (in Chinese)

[10]程须珍, Charles Y Y. 利用RAPD标记鉴定绿豆组植物种间亲缘关系. 中国农业科学, 2001, 34(2): 216-218.

Cheng X Z, Charles Y Y. Study on relationship among species inmungbean group using RAPD markers. Scientia Agricultura Sinica, 2001, 34(2): 216-218. (in Chinese)

[11]Afzal M A, Haque M M, Shanmugasundatam S. Random amplifiedpolymorphic DNA (RAPD) analysis of selected mungbean[Vignaradiata (L.) Wilczek] cultivars. Asian Journal of Plant Science, 2004, 3: 20-24.

[12]Chattopadhyay K, Ali M N, Sarkar H K, Mandal N, Bhattacharyya S. Diversity analysis by RAPD and ISSR markers among the selectedmungbean [Vigna radiata (L.) Wilczek] genotypes. The Indian Journal of Genetics & Plant Breeding, 2005, 65: 173-175.

[13]Datta S, Gangwar S, Kumar S, Gupta S, Rai R, Kaashyap M, Singh P, Chaturvedi S, Singh B, Nadarajan N. Genetic diversity in selected Indian mungbean [Vigna radiata (L.) Wilczek] cultivars using RAPD markers. American Journal of Plant Sciences, 2012, 3: 1085-1091.

[14]王丽侠, 程须珍, 王素华, 梁辉, 赵丹, 徐宁. 应用SSR标记对小豆种质资源的遗传多样性分析, 作物学报, 2009, 35(10): 1858-1865.

Wang L X, Cheng X Z, Wang S H, Liang H, Zhao D, Xu N. Genetic diversity of adzuki bean germplasm resources revealed by SSR markers. Acta Agronomica Sinica, 2009, 35(10): 1858-1865. (in Chinese)

[15]赵庆勇, 张亚东, 朱镇, 赵凌, 陈涛, 王才林. 30个粳稻品种SSR标记遗传多样性分析. 植物遗传资源学报, 2010, 11(2): 218-223.

Zhao Q Y, Zhang Y D, Zhu Z, Zhao L, Chen T, Wang C L. Analysis on genetic diversity of 30 japonica rice varieties using SSR markers. Journal of Plant Genetic Resources, 2010, 11(2): 218-223. (in Chinese)

[16]Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation and characterization of seven tetranucleotide microsatellite loci in mung-bean, Vigna radiata. Molecular Ecology Notes, 2002, 2: 293-295.

[17]Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation ofmicrosatellite markers in mungbean, Vigna radiata. Molecular Ecology Notes, 2002, 2: 96-98.

[18]Miyagi M, Humphry M, Ma Z Y, Lambrides C J, Bateson M, Liu C J. Construction of bacterial artificial chromosome libraries andtheir application in developing PCR-based markers closely linkedto a major locus conditioning bruchid resistance in mungbean. Theoretical and Applied Genetics, 2004, 110: 151-156.

[19]Gwag J G, Chung J W, Chung H K, Lee J H, Ma K H, Dixit A, Park Y J, Cho E G, Kim T S, Lee S H. Characterization of new microsatellite markers in mungbean(Vigna radiata L.). Molecular Ecology Notes, 2006, 6: 1132-1134.

[20]刘长友, 程须珍, 王素华, 王丽侠, 孙蕾, 梅丽, 徐宁. 用于绿豆种质资源遗传多样性分析的SSR及STS引物的筛选. 植物遗传资源学报, 2007, 8(3): 298-302.

Liu C Y, Cheng X Z, Wang S H, Wang L X, Sun L, Mei L, Xu N. The screening of SSR and STS markers for genetic diversity analysis of mungbean germplasm. Journal of Plant Genetic Resources, 2007, 8(3): 298-302. (in Chinese)

[21]钟敏, 程须珍, 王丽侠, 王素华, 王小宝. 绿豆基因组SSR引物在豇豆属作物中的通用性分析.作物学报, 2012, 38(2): 223-230.

Zhong M, Cheng X Z, Wang L X, Wang S H, Wang X B. Transferability analysis of mungbean genomic-SSR markers to other Vigna species. Acta Agronomica Sinica, 2012, 38(2): 223-230. (in Chinese)

[22]Porebski S, Bailey L G, Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide andpolyphenol components. Plant Molecular Biology Reporter, 1997, 15(1): 8-15.

[23]Liu K, Muse S V. PowerMarker: An integrated analysis environmentfor genetic marker data. Bioinformatics, 2005, 21(9): 2128-2129.

[24]Yeh F C, Yang R C, Boyle T. Popgene Version 1. 31. Quick UserGuide. Canada: University of Alberta, and Centre for InternationalForestry Research, 1999.

[25]施永泰, 边红武, 韩凝, 潘建伟, 童微星, 朱睦元. 中国江、浙地区栽培大麦遗传资源的RAPD研究. 作物学报, 2004, 30(3): 258-265.

Shi Y T, Bian H W, Han N, Pan J W, Tong W X, Zhu M Y. Geneticvariation analysis by RAPD of some barley cultivars in China. Acta Agronomica Sinica, 2004, 30(3): 258- 265. (in Chinese)

[26]宏棋斌, 侯磊, 罗小英, 李德谋, 肖月华, 斐炎, 杨开俊, 甲错. 应用RAPD标记分析川西北高原青稞的遗传背景. 中国农业科学, 2001, 34(2): 133- 138.

Hong Q B, Hou L, Luo X Y, Li D M, Xiao Y H, Pei Y, Yang K J, Jia C. Using RAPD for evaluating genetic background among naked barleyvarieties in Sichuan northwestern region. Scientia Agricultura Sinica, 2001, 34(2): 133-138. (in Chinese)

[27]王丽侠, 程须珍, 王素华, 刘长友, 梁辉. 小豆SSR引物在绿豆基因组中的通用性分析. 作物学报, 2009, 35(5): 816-820.

Wang L X, Cheng X Z, Wang S H, Liu C Y, Liang H. Transferability of SSR from adzuki bean to mungbean. Acta Agronomica Sinica, 2009, 35(5): 816-820. (in Chinese)

[28]徐宁, 程须珍, 王丽侠, 王素华, 刘长友, 孙蕾, 梅丽. 用于中国小豆种质资源遗传多样性分析SSR分子标记筛选及应用. 作物学报, 2009, 35(2): 219-227.

Xu N, Cheng X Z, Wang L X, Wang S H, Liu C Y, Sun L, Mei L. Screening and application of SSR molecular markers for genetic diversity analysis of Chinese adzuki bean germplasm resources. Acta Agronomica Sinica, 2009, 35(2): 219-227. (in Chinese)

[29]Sangiri C, Kaga A, Tomooka N, Vaughan D, Srinives P. Genetic diversity of the mungbean (Vigna radiata, Leguminosae) genepool on the basis of microsatellite analysis. Australian Journal of Botany, 2007, 55: 837-847.

[30]刘长友. 中国绿豆种质资源遗传多样性分析及初选核心种质构建[D]. 北京: 中国农业科学院, 2007.

Liu C Y. Analysis on genetic diversity of mungbean germplasm and primary core germplasm building in China[D]. Beijing: Chinese Academy of Agricultural Sciences, 2007. (in Chinese)

[31]周连第. 板栗种质资源遗传多样性研究[D]. 北京: 中国农业大学, 2005.

Zhou L D. Research on genetic diversity of chestnut germplasm [D]. Beijing: China Agricultural University, 2005. (in Chinese)

[32]Slatkin M. Gene flow and the geographic structure of natural populations. Science, 1987, 236(4803): 787-792.

[33]Ellstrand N C, Elam D R. Population genetic consequences of populationsize: Implications for plant conservation. Annual Review of Ecology and Systematic, 1993, 24: 217-242.
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