Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4173-4178.doi: 10.3864/j.issn.0578-1752.2013.19.023

• RESEARCH NOTES • Previous Articles    

Research on the Inhibitory Mechanism of Imazethapyr to Nodule Nitrogenase Activity in Soybean

 LI  Hui, DING  Wei, JIANG  Jun-Feng, WANG  Huai-Biao   

  1. Department of Plant Protection, Agronomy College, Northeast Agricultural University, Harbin 150030
  • Received:2013-03-25 Online:2013-10-01 Published:2013-07-18

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Abstract: 【Objective】The objective of this study is to discover the key inhibitory mechanism of imazethapyr to nodule nitrogenase activity in soybean for providing methods to resolve the toxity on nodule nitrogen fixation after imazethapyr being over used.【Method】A field experiment was conducted to determine the relationship between inhibited nodule nitrogenase activity and leghemoglobin content, ammonia content in nodule rhizobium and in nodule cytoplasm after imazethapyr pre-emergence used.【Result】Nodule nitrogenase activity was inhibited more pronounced during 21 days after treatment (DAT). One the 7 and 14 DAT, the inhibited ratio of leghemoglobin content peaked 30.52% and 35.41%, respectively at 112.5 and 25.0 g a.i./hm2 imazethapyr and then recovered to the levels of non-treated plants on the 21 and 28 DAT, respectively. The inhibited ratio of ammonia content in nodule rhizobium peaked 35.33% and 48.96%, respectively, on the 14 DAT and recovered to the levels of non-treated plants on the 28 DAT. Ammonia content in nodule cytoplasm increased more pronounced and recovered to the normal levels respectively on the 21 and 28 DAT.【Conclusion】It is an important reason that leghemoglobin content significantly decreased after imazethapyr pre-emergence applied leads to nodule nitrogenase activity inhibited, and ammonia accumulation in nodule cytoplasm also causes feedback inhibition to nodule nitrogenase.

Key words: imazethapyr , soybean , nodule nitrogenase activity , leghemoglobin , ammonia content

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