Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (4): 657-667.doi: 10.3864/j.issn.0578-1752.2013.04.001

• AGRICULTURE INFORMATION TECHNOLOGY •     Next Articles

The Molecular Identification of Transgenic Hpa110-42 Wheat and Resistance Evaluation on Fusarium Head Blight

 YANG  Min, QIN  Bao-Ping, LIU  Chang-Lai, CAI  Hong-Sheng, WANG  Zhen-Lin, LIANG  Yuan-Cun, YIN  Yan-Ping   

  1. 1山东农业大学农学院/作物生物学国家重点实验室/山东省作物生物学重点实验室，山东泰安 271018
    2.南京农业大学植物保护学院，南京210095
    3.山东农业大学植物保护学院，山东泰安 271018
  • Received:2012-11-20 Online:2013-02-15 Published:2013-01-24

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Abstract: 【Objective】This study aims to screen Fusarium head blight (FHB) resistance plants in Hpa110-42 transgenic wheat plants and provide materials for FHB resistance breeding of wheat.【Method】T2 transgenic wheat plants were identified by PCR, Southern blot and RT-PCR, assuring whether the foreign Hpa110-42 gene was integrated and expressed in transgenic wheat plants. The resistance to FHB of transgenic wheat plants was evaluated by a dripping method to single flower, and its resistance physiology was studied.【Result】PCR, Southern blotting and RT-PCR analyses have confirmed successful integration of the foreign Hpa110-42 gene into the genome of the Yangmai 158 with 1 to 3 copies and stable inheritance and expression. Disease bioassays of transgenic plants revealed that the average percentage of diseased spikelets of transgenic lines T2-17, T2-15, T2-68, T2-44 and T2-36 were highly significantly lower than YM158. Compared with Sumai 3, the average percentage of diseased spikelets of all transgenic lines was highly significantly higher than Sumai 3 excepting line T2-17. In the transgenic lines, the average percentage of diseased spikelets of line T2-17 was significantly lower than other lines, in which T2-36, T2-11 and T2-20 have reached highly significant difference level. It is showed by physiological analysis that the activities of PAL, chitinase and β-1,3-glucanase in the high resistance transgenic plants increased faster than Yangmai 158 after inoculation. The soluble protein content in all plants had a downward trend, while its content in high resistance plants is consistently higher than others. Moreover, β-1,3-glucanase activity and soluble protein content were negatively correlated with resistance grade of FHB. 【Conclusion】 The foreign Hpa110-42 gene was stably inherited, normally expressed and positively participated in the regulation of FHB resistance, and Hpa110-42 transgenic wheat plants with resistance to FHB were obtained.

Key words: Hpa110-42 , transgenic wheat plants , gene expression , Fusarium head blight , resistance physiology

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