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Table of Content

    15 February 2013, Volume 46 Issue 4
    AGRICULTURE INFORMATION TECHNOLOGY
    The Molecular Identification of Transgenic Hpa110-42 Wheat and Resistance Evaluation on Fusarium Head Blight
    YANG Min, QIN Bao-Ping, LIU Chang-Lai, CAI Hong-Sheng, WANG Zhen-Lin, LIANG Yuan-Cun, YIN Yan-Ping
    Scientia Agricultura Sinica. 2013, 46(4):  657-667.  doi:10.3864/j.issn.0578-1752.2013.04.001
    Abstract ( 550 )   PDF (614KB) ( 597 )   Save
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    【Objective】This study aims to screen Fusarium head blight (FHB) resistance plants in Hpa110-42 transgenic wheat plants and provide materials for FHB resistance breeding of wheat.【Method】T2 transgenic wheat plants were identified by PCR, Southern blot and RT-PCR, assuring whether the foreign Hpa110-42 gene was integrated and expressed in transgenic wheat plants. The resistance to FHB of transgenic wheat plants was evaluated by a dripping method to single flower, and its resistance physiology was studied.【Result】PCR, Southern blotting and RT-PCR analyses have confirmed successful integration of the foreign Hpa110-42 gene into the genome of the Yangmai 158 with 1 to 3 copies and stable inheritance and expression. Disease bioassays of transgenic plants revealed that the average percentage of diseased spikelets of transgenic lines T2-17, T2-15, T2-68, T2-44 and T2-36 were highly significantly lower than YM158. Compared with Sumai 3, the average percentage of diseased spikelets of all transgenic lines was highly significantly higher than Sumai 3 excepting line T2-17. In the transgenic lines, the average percentage of diseased spikelets of line T2-17 was significantly lower than other lines, in which T2-36, T2-11 and T2-20 have reached highly significant difference level. It is showed by physiological analysis that the activities of PAL, chitinase and β-1,3-glucanase in the high resistance transgenic plants increased faster than Yangmai 158 after inoculation. The soluble protein content in all plants had a downward trend, while its content in high resistance plants is consistently higher than others. Moreover, β-1,3-glucanase activity and soluble protein content were negatively correlated with resistance grade of FHB. 【Conclusion】 The foreign Hpa110-42 gene was stably inherited, normally expressed and positively participated in the regulation of FHB resistance, and Hpa110-42 transgenic wheat plants with resistance to FHB were obtained.
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Analysis on the Genetic Diversity in Chinese Barley Landrace and Genomic Wide Association of α-amylase Activity Genes
    JIANG Xiao-Dong, GUO Gang-Gang, ZHANG Jing
    Scientia Agricultura Sinica. 2013, 46(4):  668-677.  doi:10.3864/j.issn.0578-1752.2013.04.002
    Abstract ( 683 )   PDF (520KB) ( 740 )   Save
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    【Objective】The objective of this study is to understand genetic diversity in Chinese barley landraces and screen valuable molecular marker for α-amylase activity.【Method】 A total 257 Chinese barley landraces were genotyped with 41 SSR markers distributing on seven chromosomes of barley. Nei’s genetic distance and neighbour-joining were adoped in cluster analysis.【Result】A total of 709 alleles were identified at 41 SSR loci in 257 Chinese barley landraces. The number of alleles per locus ranged from 5 to 44, with an average of 17. The polymorphism information content value for SSRs ranged from 0.23 (Bmag 0385) to 0.94(Bmac0032) with a mean of 0.6385. Cluster analysis detected that nine clusters were identified based on Nei’s genetic distance in 257 accessions. Association analysis showed that five SSR markers significantly associated with α–amylase activity. 【Conclusion】 Chinese barley landraces have rich genetic diversity and could be clustered into nine different groups based on morphological characteristics, geographic origin and genetic relationships. Among the five SSR markers associated with α–amylase activity, Bmag0385 on chromosome-7H carrying allele A215 possessed the highest α-amylase activity, and Bmac0273 on 7H carrying with allele A141 had relatively high positive effect on α-amylase activity. All of them could be used in marker-assisted selection for malting barley breeding.
    Functional Analysis of T1N6_22 in Arabidopsis thaliana Against the Infection of Pst DC3000
    HAO Cong-Cong, ZHENG Hui-Xin, JIA Jiao, SI He-Long, CHEN Zhan, ZHAO Bin, ZHANG Jing, XING Ji-Hong, DONG Jin-Gao
    Scientia Agricultura Sinica. 2013, 46(4):  678-685.  doi:10.3864/j.issn.0578-1752.2013.04.003
    Abstract ( 614 )   PDF (421KB) ( 1440 )   Save
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    【Objective】 The objective of this study is to investigate the function of the T1N6_22, a resistant gene of Arabidopsis against B. cinerea, in Arabidopsis resistance to Pseudomonas syringae pv. tomato DC3000, to further analyze the mechanism of T1N6_22 genes in Arabidopsis resistance to Pst DC3000. 【Method】 The symptoms, increase of the content of callose and bacterial concentration of the t1n6_22 and t1n6_22/T1N6_22 in plants inoculated with Pst DC3000 were investigated to study the function of the T1N6_22 in Arabidopsis resistance to Pst. DC3000. RT-PCR technology was used to analyze the expression of T1N6_22 in Col-0 treatment with SA, JA, ET and the expression of defence-related genes in Col-0, the t1n6_22 and t1n6_22/T1N6_22 plants. Quantitative real-time PCR technology was used to analyze the expression of T1N6_22 and the key genes involved in the SA, JA/ET signal pathway in Col-0 inoculated with Pst DC3000. 【Result】 The t1n6_22 exhibited enhanced resistance to Pst DC3000, the Col-0 and complemental plants showed an obvious susceptibility to Pst DC3000. The expression of T1N6_22 in Col-0 treatment with SA was significantly enhanced, suggesting the expression of T1N6_22 induced by SA. Compared with the Col-0 and t1n6_22/T1N6_22 plants, the expression of PAL, PR4, PPO, SOD and CAT genes were downregulated in the t1n6_22 plants. The expression of T1N6_22 and the key genes involved in the SA, JA/ET signal pathway, such as PR1, PR3, PR5 and PDF1.2 genes, were upregulated in Col-0 after inoculation of Pst DC3000.【Conclusion】T1N6_22 gene is a negative regulatory component of Arabidopsis against Pst DC3000 and the T1N6_22 gene expression is induced by SA. T1N6_22 may be involved in the regulation of plant secondary metabolites in impact of the resistance to Pst DC3000 in Arabidopsis.
    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY
    The Productive Advantages and Formation Mechanisms of “Indica Rice to Japonica Rice”
    ZHANG Hong-Cheng, ZHANG Jun, GONG Jin-Long, CHANG Yong, LI Min, GAO Hui, DAI Qi-Gen, HUO Zhong-Yang, XU Ke, WEI Hai-Yan
    Scientia Agricultura Sinica. 2013, 46(4):  686-704.  doi:10.3864/j.issn.0578-1752.2013.04.004
    Abstract ( 667 )   PDF (635KB) ( 1025 )   Save
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    【Objective】 The objective of this study was to reveal the productive advantages of “indica rice to japonica rice” and summarize its physiological ecological mechanisms systematically from the viewpoint of cultivation. 【Method】 A field experiment was conducted with representative japonica rice varieties (Wuyunjing24, Xudao3, Lianjing7, Xuyou733, etc.) and indica rice varieties (Liangyoupeijiu, Ⅱyou084, Xinliangyou6380, Yangliangyou6, etc.) as materials in rice-wheat cropping areas (Yangzhou, Xinghua, Donghai of Jiangsu) in the lower reaches of the Yangtze River in 2010-2011. Difference of growth security, grain yield, quality, utilization of temperature and solar radiation, dynamics of population growth, plant type and lodging characteristics between japonica rice and indica rice were studied systematically. 【Result】 Under the simplified cultivation conditions of mechanical and swing transplanting, the growth security of japonica rice was better than indica rice, for japonica rice possessed longer milky stage and the resistance to low temperature at the later period compared to indica rice. The average yields of japonica rice in both years were, 10.90 and 10.87 t•hm-2 in Yangzhou, 10.93 and 10.65 t•hm-2 in Xinghua, 10.49 and 10.41 t•hm-2 in Donghai, higher than indica rice significantly. Grain filling characteristics of japonica rice exceeded indica rice, which was the key point of harvesting high yield of japonica rice, with the roughly same level of population spikelets or slightly less. Milling quality and cooking and eating quality of japonica rice were better than indica rice significantly, while the appearance and nutritional quality followed an opposite tendency accordingly. The physiological ecological characteristics of productive advantages of “indica rice to japonica rice” were as follows. Firstly, growth duration of japonica rice was longer than indica rice significantly and japonica rice was more adapted to cool weather at the later period of grain filling, increasing utilization of temperature and solar radiation and ensuring japonica rice maturing safely. Secondly, strong photosynthetic capacity of japonica rice at the later period increased dry matter weight and total filling. Thirdly, japonica rice improved the resistance to cold and premature senescence, and lodging due to strong root, stem and sheath, guaranteeing the enrichment and support of large sink. 【Conclusion】 Under the mechanizing simplified cultivation conditions, japonica rice, not only matured safely with strong growth advantages at the later period, but also achieved high grain yield, quality, efficiency and comprehensive productivity because of making full use of temperature and solar radiation in comparison to indica rice.
    A Preliminary Study of Integrated Assessment of Wheat Natural Disaster Risk in China
    ZHAO Jun-Ye, ZHANG Qiao, ZHAO Si-Jian
    Scientia Agricultura Sinica. 2013, 46(4):  705-714.  doi:10.3864/j.issn.0578-1752.2013.04.005
    Abstract ( 502 )   PDF (495KB) ( 819 )   Save
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    【Objective】In order to explore the integrated assessment method of crop natural disaster risk, comprehensive evaluation and analysis of wheat natural disaster risk on the provincial units were carried out.【Method】 Based on the regional natural disaster theories, crop natural disaster integrated risk assessment index system was established. Using the analytic hierarchy process (AHP) and differentiated indexes weighted methods, the integrated assessment index of wheat natural disaster risk (I) was acquired, by which integrated assessment and zoning of wheat natural disaster risk was carried out, and the assessment results was verified by the estimated value of wheat yield loss (L) due to disasters.【Result】Based on frequency data of different natural disasters with wheat as hazard-bearing body, the components and hazard of disaster-causing factors of different provinces were analyzed. Forty indexes were chosen to appreciate stability of disaster environment (S), hazard of disaster-causing factor (H), vulnerability of wheat (V) and capability of disaster defending and resistance (C), which were compared within different provinces, and the risk integrated index I was assessed by the assessment model. The risk map of wheat natural disaster risk was drawn based on GIS, which showed that the higher risk regions mainly concentrated in Northwest wheat zone, Huanghuai wheat zone, and mid lower reaches of the Yangtze River wheat zone. The assessment result was found to be significantly correlated with the result of wheat yield loss (L) estimated.【Conclusion】The assessment method and results can be used to explore and develop integrated strategies including risk monitoring, forecasting, defending and resistance so as to mitigate wheat production risks.
    甘蓝型油菜黄化突变体的叶绿体超微结构、 气孔特征参数及光合特性
    XIAO Hua-Gui, YANG Huan-Wen, RAO Yong, YANG Bin, ZHU Ying, ZHANG Wen-Long
    Scientia Agricultura Sinica. 2013, 46(4):  715-727.  doi:10.3864/j.issn.0578-1752.2013.04.006
    Abstract ( 642 )   PDF (1030KB) ( 1109 )   Save
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    【Objective】 In order to discuss the yellowing mechanism and provide a theoretical basis in rape breeding practice, the relationship between chloroplast ultrastructure, stomatal characteristic parameters, photosynthetic pigment content and photosynthetic characteristics of the spontaneous chlorophyll-reduced mutant NY in B. napus L. were studied.【Method】Taking the rape mutant NY, wild type NG, F1 (NY×NG) and rF1(NG×NY) as research materials, the heart-leaf and the flatten-leaf at five-leaf stage were used for chloroplast ultrastructure observation, stomatal characteristic parameters investigation, photosynthetic pigment content determination, photosynthetic characteristics measurement and agronomic traits investigation.【Result】In general, the chloroplast development degree in yellow heart-leaf and yellow-green flatten-leaf from mutant NY was worse than that of wild type NG, F1 (NY×NG) and rF1(NG×NY) from chloroplast ultrastructure; the chloroplast numbers of heart-leaf in a lower epidermis stoma guard cell from mutant NY was decreased about 40%, whereas the number in yellow-green flatten-leaf was close to that of the wild-type NG in stomatal characteristic parameters; the Chla, Chlb, Chl(a+b), carotenoids and the net photosynthetic rate of mutant were significantly lower than those of the same period of wild-type and F1, rF1 in content and composition of photosynthetic pigment as well as photosynthetic characteristics. Correspondingly, the growth period delayed, economic traits deteriorated and grain yield per plant decreased, which happened in the mutant, but the above agronomic traits and photosynthetic characteristics from F1 and rF1 were restored to normal levels.【Conclusion】The mutant NY is one of total chlorophyll deficiency type caused by chloroplast structure developmental defects. Abnormal chloroplast structure, less grana and grana lamella and lower chlorophyll content are main reasons for mutant lower net photosynthetic rates and worst agronomic traits.
    PLANT PROTECTION
    Characteristics of Mating Types, Physiological Races, Metalaxyl Sensitivity and mtDNA Haplotypes of Phytophthora infestans in Sichuan Province
    LI Hong-Hao, PENG Hua-Xian, XI Ya-Dong, WANG Xiao-Li, LIU Bo-Wei
    Scientia Agricultura Sinica. 2013, 46(4):  728-736.  doi:10.3864/j.issn.0578-1752.2013.04.007
    Abstract ( 572 )   PDF (593KB) ( 609 )   Save
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    【Objective】The objective of this study is to provide a scientific basis to control potato late blight through the systematical analysis of populations of Phytophthora infestans. 【Method】The mating types, metalaxyl sensitivity, physiological races and mtDNA haplotypes of P. infestans isolates collected in Sichuan during 2008 to 2011 were analyzed. 【Result】Most P. infestans isolates in Sichuan were A2 mating type with the frequency of 62.5%. The frequency of A1 mating type and self-fertility in the isolates were 18.8% and 18.4%, respectively. The isolates of A1 mating type were only detected in Jiulong and Puge Counties. Fifty-five races were identified among 192 isolates and race 1.2.3.4.5.6.7.8.9.10.11 were the dominant races in Sichuan. There were 99.48% isolates contained at least two virulence genes. The sensitivity test to metalaxyl showed that resistant, intermediate and sensitive isolates were detected in Sichuan with the frequency of 63.0%, 22.0% and 15.0%, respectively. The sensitive isolates distributed in Puge, Daofu and Jiulong Counties. Two mtDNA haplotypes,Ⅰa and Ⅱa, were detected from populations of P. infestans in Sichuan with the frequency of 97.4% and 2.6%, respectively, which belonged to the “new” populations, following the second global migration of the pathogen.Ⅰa haplotype distributed in all potato producing areas extensively, including A1 mating type, A2 mating type, self-fertility and unknown mating type isolates. 【Conclusion】The composition of populations of P. infestans is getting more complex in Sichuan. To control potato late blight scientifically it is necessary to explore new resistant germplasms, breed horizontal resistant varieties, dispose resistant varieties reasonably and take full advantage of the potato late blight forecasting system.
    Effects of Temperature on the Distribution of the Colorado Potato Beetle (Leptinotarsa decemlineata)—Effect of High Temperature on Its Emergence in Turpan, Xinjiang
    LI Chao, CHENG Deng-Fa, LIU Huai, ZHANG Yun-Hui, SUN Jing-Rui
    Scientia Agricultura Sinica. 2013, 46(4):  737-744.  doi:10.3864/j.issn.0578-1752.2013.04.008
    Abstract ( 542 )   PDF (797KB) ( 457 )   Save
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    【Objective】 The objective of this study is to establish a causal relationship between dispersal of Colorado potato beetle, Leptinotarsa decemlineata (Say) and temperature, suggesting that temperature is the key limiting factor preventing Colorado potato beetle to colonize in Turpan area, Xinjiang, China. And to provide an empirical basis for the sustainable management of Colorado potato beetle. 【Method】 The emergency rates of the Colorado potato beetle larvae were observed under different temperatures and different times. And the critical high-temperature tolerance of Colorado potato beetle emergence was obtained. Combined with the historical climate data of Xinjiang, spatial analysis function of ArcGIS software was used to create a distribution map of the daily mean maximum temperatures of the Xinjiang in summer. 【Result】 With the increasing of temperature, the eclosion rate of Colorado potato beetle larvae was gradually decreased, and the developmental duration was gradually extended. When the temperature reached 39℃, the emergence rate approached zero, the critical high temperature of Colorado potato beetle eclosion tolerance was 39℃, and at the same time, the tolerance duration at temperature of 39℃ was 72 h. 【Conclusion】 Summer temperature in Turpan area can rise above 39℃ and lasts for more than a month. The extreme heat prevents Colorado potato beetle from colonizing in local areas and limits beetle’s dispersal eastward. It is necessary to strengthen the inspection and quarantine measures to prevent spreading of Colorado potato beetle eastward through human-facilitated transmission. This precautionary measure is critical for the sustainable production of potatoes and other Solanaceae crops.
    Toxicity and Persistence of the Insecticide Spirotetramat on the Peach Aphid (Myzus persicae)
    JIANG Chun-Yan, GONG Ya-Jun, SHI Bao-Cai, WANG Ze-Hua, KANG Zong-Jiang, GU Yun, ZHU Liang, WEI Shu-Jun
    Scientia Agricultura Sinica. 2013, 46(4):  745-755.  doi:10.3864/j.issn.0578-1752.2013.04.009
    Abstract ( 676 )   PDF (786KB) ( 710 )   Save
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    【Objective】The objective of this study is to evaluate the toxicity and persistence of the spirotetramat, a new two-way systemic insecticide, to the peach aphid (Myzus persicae).【Method】Three bioassay methods were tested in the laboratory. The toxicity was analyzed using probit analysis and time-dose-mortality. Additionally, the persistence of this pesticide was studied using clip-cage method.【Result】The bioassay results differed among the three bioassay methods, i.e. clip-cage, triplex-glass and round-leaf methods. The clip-cage method kept the leaf the longest time and generated more reliable result. The mortality of the aphid nymph increased with the increasing of detecting time, reached the highest value at 120 h after spray. The probit analysis showed that the LC50 of triplex-glass, clip-cage and round-leaf method was 0.0886, 0.1258 and 0.8459 mg•kg-1, respectively. The time-dose-mortality analysis showed that, at 1.5625 mg•kg-1, LT50 of triplex-glass, clip-cage and round-leaf method was 2.5, 2.9 and 3.5 d, respectively. The LT50 decreased with the increasing of the concentration of the spirotetramat. The spirtetramat had a long time of persistence. The mortality of the aphid nymph decreased from 100% to less than 20% after spray for seven days.【Conclusion】The study suggests that the clip-cage for the bioassay of the spirotetramat will contribute to the bioassay method of those pesticides with long time of persistence. The peach aphids collected from the field of Beijing area of China are sensitive to the spirotetramat.
    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Effects of Different Carbon and Nitrogen Managements on Yield, Straw Decomposition, Soil CO2 Flux of the Winter Wheat / Summer Maize
    HUANG Tao, CHOU Shao-Jun, DU Juan, SHI Zhen-Xia, JU Xiao-Tang
    Scientia Agricultura Sinica. 2013, 46(4):  756-768.  doi:10.3864/j.issn.0578-1752.2013.04.010
    Abstract ( 613 )   PDF (699KB) ( 955 )   Save
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    【Objective】 The responses of different carbon and nitrogen managements to the winter wheat/summer maize rotation system were systematically studied in North China Plain. It was expected that the research results would provide a theoretic basis and data support for high yield, soil fertility and environment-friendly “triple-win” goal.【Method】The methods of Nmin test, buried nylon mesh bag and static alkali absorption were adopted for study the effects of different nitrogen fertilizer managements on nitrate accumulation in 0-1 m soil profile, straw decomposition and soil CO2 flux in different periods of winter wheat/summer maize season, respectively.【Result】The results showed that not only the ratios of optimized treatments and balanced treatments to conventional treatments were 100.8%-115.9% for winter wheat yield, and 96.0%-116.4% for summer maize yield, but also could save 48.2%-70.4% nitrogen fertilizer input. The 0-1 m nitrate accumulation from the conventional treatments was the highest, which could reach to 456.7 and 419.8 kgN•hm-2. But the highest 0-1 m nitrate accumulation from the optimized treatments and balanced treatments were only 283.3 and 180.6 kgN•hm-2, respectively. Hence, the soil nitrate leaching risk in the conventional treatments was higher than the optimized treatments and balanced treatments. The decomposition of maize straw in the cold and arid winter wheat season was slow, which the decomposition rate was 61.7%-70.1%. The decomposition of wheat straw in the hot and moist summer maize season was fast, which the decomposition rate was 56.7%-79.3%. Soil CO2 flux had obvious seasonal variation, which the average daily CO2 flux in the winter wheat season was 4.8-10.8 gC•m-2, but 12.7-20.7 gC•m-2 in the summer maize season. The accumulated soil CO2 flux from the balanced treatments with organic fertilizer were the highest, which were 3 844.2 and 4 642.3 gC•m-2, respectively. Furthermore, it was significantly higher than the other treatments.【Conclusion】The optimized and balanced C & N managements not only reduced N fertilizer inputs, stabilized crop production but also decrease a the 0-1 m soil nitrate accumulation and cultivating soil fertility.
    Research on the Structural Characteristics of Promoted-Release Phosphate Rocks and Its Phosphorus Bioavailability Mechanism
    HUANG Lei, MAO Xiao-Yun, WANG Jun, DENG Bing-Lu, WANG Guan-Hao, LIAO Zong-Wen
    Scientia Agricultura Sinica. 2013, 46(4):  769-779.  doi:10.3864/j.issn.0578-1752.2013.04.011
    Abstract ( 680 )   PDF (976KB) ( 445 )   Save
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    【Objective】The objective of the study is to disclose the structural characteristics and their internal relation with phosphorus validity in the promoted-release phosphate rocks (PRPr), and discuss the method for quantitative determination of active phosphorus.【Method】Continuous water extraction (CWE) and digestion with acetic acid and sodium acetic mixture (ASA) were involved, and the re-activated experiments and spectroscopic techniques were carried out to explore the phosphorus validity mechanism of PRPr from promoted-release persistence and molecular structure.【Result】The water-soluble phosphorus and active phosphorus in PRPrs treated by sodium lignosulphonate (SL) increased significantly. Compared with the phosphate rocks, the accumulative release rates of phosphorous of four PRPrs were 14.0, 5.6, 8.8 and 5.1 times higher and active phosphorus in PRPrs increased by 9.8%-93.1%, respectively. The results of infrared spectrum (IR) and X-ray diffraction(XRD)analysis showed that the characterastic abasorption spectrum of H2PO42- was strengthened or new characterastic absorption spectrum of H2PO42- appeared, the structure of apatite crystal was destroyed and amorphous apatite increased. These constantly made insoluble and citrate-soluble phosphorus in phosphate rock changed into active status and improved the bioavailability of phosphorus.【Conclusion】The promoted-release treatment could significantly improve the bioavailability of phosphorus in phosphate rock through constantly translate insoluble and citrate-soluble phosphorus into active status. The continuous water extraction and ASA can be used to make a proper assessment on bioavailability of PRPrs.
    HORTICULTURE
    Isolation and Identification of NBS-LRR Resistance Gene Analogs Sequences from Vitis davidii
    ZHANG Ying, LI Feng, LIU Chong-Huai, FAN Xiu-Cai, SUN Hai-Sheng, JIANG Jian-Fu, ZHANG Guo-Hai
    Scientia Agricultura Sinica. 2013, 46(4):  780-789.  doi:10.3864/j.issn.0578-1752.2013.04.012
    Abstract ( 544 )   PDF (1059KB) ( 725 )   Save
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    【Objective】 The purpose of this study was to get white rot disease resistant homologous clips from Vitis davidii ‘Vitis davidii cv. Gaoshan 2’, which will provide an effective means for screening white rot disease resistant gene. 【Method】 According to the known plant resistance genes NBS-LRR conservative area, degenerate primers were designed and used to clone targeted resistance gene analogs using templates of genomic DNA and cDNA from white rot disease resistant variety of Vitis davidii ‘Vitis davidii cv. Gaoshan 2’. Then the gene expression analysis was performed. 【Result】 Ten NBS-LRR kinds of resistance gene analogs from Vitis davidii ‘Vitis davidii cv. Gaoshan 2’ were obtained, and their amino acid sequences showed rich polymorphism. Further analysis found that among the ten NBS sequences, four genes belong to non-TIR-NBS-LRR kind of resistance genes, six belong to TIR-NBS-LRR kind of resistance genes. Quantitative PCR analysis showed that NB7 gene was induced by white rot fungus, and NB7 gene was less abundantly expressed in the leaves of Vitis davidii, NBS6 gene is down-regulated expression after induced by white rot disease. 【Conclusion】 Ten RGAs were successfully obtained from Vitis davidii, and fluorescence quantitative PCR analysis showed that, both NB7 gene and NBS6 gene expression are induced by white rot fungus. The results of this study have layed a foundation for final cloning white rot disease resistant gene from Vitis.
    Qualitative and Quantitative Analysis of Floral Volatile Components from Different Varieties of Lilium spp.
    ZHANG Hui-Xiu, HU Zeng-Hui, LENG Ping-Sheng, WANG Wen-He, XU Fang, ZHAO Jing
    Scientia Agricultura Sinica. 2013, 46(4):  790-799.  doi:10.3864/j.issn.0578-1752.2013.04.013
    Abstract ( 858 )   PDF (567KB) ( 1085 )   Save
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    【Objective】The aim of this experiment was to analyze the floral volatile composition and emission, and find out the key aroma components of Lilium spp.【Method】Dynamic headspace collection and ATD-GC/MS (Auto Thermal Desorber-Gas Chromatography/Mass Spectrometry) were used to qualitatively and quantitatively analyze the floral volatile components of seven varieties of Lilium spp. from four lily hybrids. 【Result】A total of 64 floral volatile compounds were identified from Lilium spp, 39 compounds were identified from 3 varieties of Asiatic hybrids and Longiflorum×Asiatic hybrids with weak fragrance, 54 compounds from 4 varieties of Oriental hybrids and Longiflorum hybrids with strong fragrance. The results showed that the floral volatile components were significantly different among lily hybrids. The amount of hydrocarbons compounds were the highest in Asiatic hybrids and Longiflorum×Asiatic hybrids, the main volatile components were 2-ethyl-1-hexanol, ethylbenzene, o-xylene, 2,2,4,6,6-pentamethyl-heptane, 5-ethyl-2,2,3-trimethyl-heptane, 3-methyl-undecane and 2,2,6-trimethyl decane. But the main components released from the flowers of Oriental hybrids and Longiflorum hybrids were terpenes,including β-myrcene, β-ocimene, linalool and 2-ethyl-1-hexanol.【Conclusion】Terpenes and benzoic acid methyl ester were not detected from 3 lily varieties with weak aroma, while it was found that terpens were released with the highest amounts in 4 lily varieties with strong aroma. β-ocimene and linalool were the main terpenes and may be identified as key aroma components of lily flowers.
    STORAGE·FRESH-KEEPING·PROCESSING
    The “Value” Expression Pattern and Technical Procedure for Genetically Modified Organism Detection Reference Materials
    LI Yun-Jing, SONG Gui-Wen, SHEN Ping, ZHAO Xin, ZHOU Yun-Long
    Scientia Agricultura Sinica. 2013, 46(4):  800-809.  doi:10.3864/j.issn.0578-1752.2013.04.014
    Abstract ( 551 )   PDF (572KB) ( 709 )   Save
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    This paper describes the worldwide production, characterization and application status of genetically modified organism (GMO) detection reference materials (RMs). The different "value" expression patterns and technical approaches of current GMO detection RMs were systematically compared. The advantages and disadvantages of each "value" expression pattern were also elucidated. According to the comparison results, it was suggested that the most suitable pattern of "value" expression and technical approaches of RMs for GMO safety supervision in China. These will provide references for the establishment of characterization system and production and application platform for GMO detection RMs.
    Molecular Identification and rDNA-ITS Sequence Analysis of Fungal Pathogens in Kiwifruit During Storage
    DUAN Ai-Li, LEI Yu-Shan, SUN Xiang-Yu, GAO Gui-Tian, ZHAO Jin-Mei, GU Liu-Jie
    Scientia Agricultura Sinica. 2013, 46(4):  810-818.  doi:10.3864/j.issn.0578-1752.2013.04.015
    Abstract ( 643 )   PDF (751KB) ( 978 )   Save
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    【Objective】The fungal pathogens of ‘China optimal’, ‘Haiwode’ and ‘Qinmei’ kiwi fruit that were harvested in Zhouzhi County, Shaanxi Province, were isolated during storage and identified by rDNA-ITS sequences analysis.【Method】With rDNA-ITS analysis and morphological characterization, the species of Fungal pathogens were differentiated and identified. By means of the genetic relationship analysis and multiple comparison analysis of ITS sequences of fungal pathogens, the diversity of intra-species in the pathogens were investigated.【Result】Five strains were identified, e.g. Penicillium, Trichoderma, Alternaria, Mucor and Simplicillium. Penicillium was the dominant species, accounting for 63.3% of the tested strains. And Trichoderma was the second dominant species, accounting for 26.7%. There were little differences in ITS sequences among Trichoderm, approval ratings were 99%. But the differences of ITS sequences between the Penicillium were significant .Such as Penicillium sp., Penicillium purpurogenum, Penicillium chrysogenum, Penicillium paneum and Penicillium commune.【Conclusion】Penicillium are mainly fungal pathogens of ‘China optimal’, ‘Haiwode’ and ‘Qinmei’ kiwifruit that were harvested in Zhouzhi County, Shaanxi Province. The differences of rDNA-ITS sequences between Penicillium are obvious. So, rDNA-ITS sequence between ourtra-species and intra-species have a certain reference value.
    ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT
    Progresses in Research of Genome-Wide Association Studies in Livestock and Poultry
    WANG Ji-Ying, WANG Hai-Xia, CHI Rui-Bin, GUO Jian-Feng, WU Ying
    Scientia Agricultura Sinica. 2013, 46(4):  819-829.  doi:10.3864/j.issn.0578-1752.2013.04.016
    Abstract ( 630 )   PDF (609KB) ( 2708 )   Save
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    Genome-wide association study (GWAS) is a new research method for complex traits developed in recent years. In the last several years, many GWAS were performed focusing on economically important traits, genetic defect diseases, resistant or susceptible ability to complex diseases, breed characteristics of the main livestock and poultries. These studies not only enlarged molecular markers used in the marker-assisted selection, but provided important information for elaborate the genetic mechanism of these traits. In this study, the populations, main statistical methods and results of the GWAS in main livestock and poultry were reviewed. Furthermore, some prospect of GWAS in the future was made. This review will provide reference for further research on genetic background of the important traits of livestock and poultry by GWAS.
    Molecular Cloning and Expression Pattern of Duck Serum Albumin Gene During Viral Infection
    XU Qi, CHEN Yang, LI Xiu, HUANG Zheng-Yang, ZHANG Yang, LI Xin-Yu, TONG Yi-Yu, DUAN Xiu-Jun, CHEN Guo-Hong
    Scientia Agricultura Sinica. 2013, 46(4):  830-840.  doi:10.3864/j.issn.0578-1752.2013.04.017
    Abstract ( 663 )   PDF (1546KB) ( 581 )   Save
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    【Objective】This experiment was conducted to clone serum albumin (ALB) gene and explore albumin gene molecular structure and expression regularity in duck. 【Method】Screening albumin as a candidate gene based on the results of suppression subtractive hybridization (SSH). The disease models infected duckling virus hepatitis and polyriboinosinic polyribocytidylic acid, Poly(I:C) were constructed. The cDNA and 5′ flanking sequence of ALB were obtained by RT-PCR, RACE and genome walking, and then the sequences were analyzed by bioinformatics approaches. The temporal spatial expression of ALB in tissues was detected by RT-qPCR. The results showed that the full-length cDNA of ALB gene was 2 107 bp, including 47 bp of 5′UTR, 212 bp of 3′UTR, and 1 848 bp of open reading frame (ORF). And the potential transcriptional factor binding sites were predicted in 5′ flanking region including TAAT box, CAAT box, HSF, HNF, C/EBP and so on in liver. The results of RT-qPCR showed that ALB mRNA was specifically expressed in liver, the ALB mRNA levels of livers decreased first, then increased and remained stable 24 hours late in the 3-day-old ducklings treated with duckling virus hepatitis and Poly(I:C). 【Conclusion】The cDNA and 5′ flanking sequence of ALB were detected successfully in duck, its genetic relationship with other kinds of flow were closing by constructing phylogenetic tree. The albumin gene expression regularity was discovered after infected duckling virus hepatitis and Poly(I:C).
    Selection of Excellent Germplasm Resources of Elymus nutans in Alpine Region
    CHEN Hong-Yang, FU Hua, HUANG De-Jun, ZHOU Hong-Jin
    Scientia Agricultura Sinica. 2013, 46(4):  841-848.  doi:10.3864/j.issn.0578-1752.2013.04.018
    Abstract ( 685 )   PDF (594KB) ( 523 )   Save
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    【Objective】 This study aimed to provide basic materials for parents matching and synthetic variety breeding of E. nutans with high yield and good quality in alpine region. 【Method】 Through field experiments, hay yield traits, crude protein (CP), water soluble carbohydrate (WSC), neutral detergent fiber (NDF) and acid detergent fiber (ADF) of 11 E. nutans populations from Qinghai-tibet plateau were investigated and appraised. 【Result】 The variations of eleven E. nutans populations were great in hay yield characters and nutritive qualities, the coefficient of variation of hay yield is the largest, which is 52.13%, while the variation coefficient of NDF is the smallest, which is 3.97%. A single character comparison between different populations showed that P8 had high hay yield, being 2971.66 kg•hm-2, while the highest CP was 22.61% for P1, which also has the lowest NDF and ADF, being 59.75% and 36.26%, respectively; P3, P4, P5, P6 and P7 had high sugar content, being 5.18%, 4.46%, 5.53%, 5.79% and 6.15%, respectively. 【Conclusion】 Based on the single character, P8 can be used as the parent material of high yield, P1 and P9 can be used as the parent materials of high nitrogen and low fiber, respectively, while P3, P4, P5, P6 and P7 can be used as the parent materials of high sugar, the mentioned materials all can participate in the conventional hybrid breeding. Based on yield and nutrient quality characters, P1 is of middle yield, high nitrogen and low fiber material, P2 is of high yield and middle nitrogen materials, P8, P9 and P10 are of high yield and middle sugar materials, while P3 and P4 is of middle yield and high sugar materials. The above mentioned materials as high yield and good quality materials under selective breeding can be used to develop new varieties rapidly.
    RESEARCH NOTES
    Characterization of a Dehydrin Gene TaDHN-1 and Its Response to Abiotic Stresses in Wheat
    ZHANG Ning, SUN Min-Shan, LIU Lu-Lu, MENG Fan-Rong, REN Jiang-Ping, YIN Jun, LI Yong-Chun
    Scientia Agricultura Sinica. 2013, 46(4):  849-858.  doi:10.3864/j.issn.0578-1752.2013.04.019
    Abstract ( 652 )   PDF (718KB) ( 704 )   Save
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    【Objective】 Abiotic stresses are major factors which significantly affecting the growth and yield of crop plants. In this study, the objective is to characterize a dehydrin gene and its response to abiotic stresses, such as drought, high-salt, cold and heat. The biological functions of the dehydrin gene during the stress tolerance of wheat plant were also discussed, which would provide important information for the application of the dehydrin gene in wheat molecular breeding focused on the improvement of stress tolerance.【Method】RT-PCR technology was used in cDNA cloning of TaDHN-1 and proper bioinformatic methods were applied for characterizing the dehydrin gene and its deduced protein. The gene expression patterns were analyzed by using the real time qRT-PCR. 【Result】A wheat dehydrin gene TaDHN-1 was cloned and sequencing analysis showed that the cDNA of the dehydrin gene is 487 bp in length, and encodes 112 amino acids with a predicted molecular weight of about 11.5 kD and pI of 6.6. Amino acid sequence analysis indicated that the TaDHD-1 included a conserved C-terminal K-segment and was sorted into Kn subfamily of dehydrins. Protein structural analysis demonstrated that the region of loops accounted for 82.1% of the whole protein and this feature was related to the higher hydrophilia of the dehydrin. PredictProtein analysis showed that no transmemberane region was detected in TaDHN-1 and it was located in cytoplasm. The expression analysis revealed that TaDHN-1 was induced by plant hormone ABA, as well as by drought-, salt- and chilling- stresses, while insensitive to the heat stress in wheat plants. Interestingly, the expression level of TaDHN-1 was gradually decreased with the development of wheat kernels and the expression was very weak at later period of seed maturation, which indicated that TaDHN-1 was not involved in the dehydration protection during the maturation of wheat seeds. 【Conclusion】 The wheat dehydrin gene TaDHN-1 belongs to the Kn subclass of plant dehydrin family, and it was involved in the ABA dependent stress-responding pathway. It was deduced that TaDHN-1 plays important roles during the stress tolerance of drought, high-salt and cold, while is insensitive to heat stress and not involved in the dehydration protection during the maturation of wheat seeds.
    Proteomic Analysis of Tobacco Rosette Stage Leaves Under Different Climatic Conditions
    CAI Yong-Zhan, ZHOU Pu-Xiong, LI Fu-Lin, ZHAO Chang-Ling, LIN Chun, YANG Huan-Wen, MAO Zi-Chao
    Scientia Agricultura Sinica. 2013, 46(4):  859-870.  doi:10.3864/j.issn.0578-1752.2013.04.020
    Abstract ( 519 )   PDF (806KB) ( 725 )   Save
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    【Objective】 The effects of different climatic conditions on the molecular developmental mechanism of rosette stage tobacco were studied. 【Method】 Under the same conditions of soil, cultivation and management, flue-cured tobacco cultivar Yun 87 was pot-planted in the farm of Yunnan Agricultural University in Kunming and Jinzhuang of Yulong county in Lijiang, respectively. When tobacco developed into the rosette stage, the agronomic traits, the SPAD values and the net photosynthetic rate were determined, simultaneously, total proteins of the tobacco leaves were separated by two-dimensional electrophoresis(2-DE) and the more than 2 times differential expression proteins were analyzed through MALDI-TOF-MS. 【Result】 Compared with the Kunming experimental site, the tobaccos planted in Jingzhuang had the higher SPAD value, net-photosynthetic rate and the speeding of developmental process resulted in the increase of the plant height, length of inter stem nodes, stem circumference, leaf width as well as leaf area. The proteomic analysis shown that the leaves of tobaccos planted in both experimental sites had 39 protein spots with more than 2 times differential expression, in which 33 were identified and annotated. Among the annotated ones, compared with Kunming site, tobaccos of Jingzhang site had 3 (9.09%) specifically expressed, 7 (21.21%) up-regulated, 19 (57.57%) down-regulated proteins, whilst the tobaccos planted in Kunming had 4 (12.12%) specific proteins. The functional classification of the differentially expressed proteins belonged to tobacco metabolism of photosynthesis, defense or (and) stress tolerance , chloroplast protein synthesis, mineral metabolism and so on.【Conclusion】 Compared with rosette stage tobaccos of Kunming, the counterparts of Jingzhuang had higher SPAD value, net-photosynthetic rate,and larger agronomic traits, such as plant height, length of inter stem nodes, stem circumference, leaf width as well as leaf area, and those performance was consistent to that the Jingzhuang tobaccos accumulated proteins related to higher photosynthetic efficiency, whilst the Kunming tobaccos enriched the proteins for improving stress tolerance, chloroplast protein biosynthesis and mineral metabolism as well. The results preliminarily illuminated the molecular mechanism of Lijiang tobacco development with characteristics which contains much more total sugars and reduction sugars , while less total nitrogen and nicotine.