Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (22): 4552-4560.doi: 10.3864/j.issn.0578-1752.2012.22.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Detection of Quantitative Trait Loci for Plant Height in Different Environments Using an RIL Population in Cucumber

 MIAO  , HAN  , GU  Xing-Fang, ZHANG  Sheng-Ping, ZHANG  Zhong-Hua, HUANG  San-Wen, WANG  , YE    

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture, Beijing 100081
  • Received:2012-01-19 Online:2012-11-15 Published:2012-09-26

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Abstract: 【Objective】Plant height is related closely to cucumber (Cucumis sativus L.) yield and plant development. QTL analysis for the traits related to plant height in cucumber can provide a basis for fine mapping and gene cloning. The result not only provide a theoretical basis for molecular assistant selection (MAS) breeding in plant morphogenesis and high yield, but also integrate research results of plant height.【Method】Phenotypic data of 148 F9 recombinant inbred lines (RILs) which originated from a narrow-cross between 9110Gt and 9930 were investigated four times in different seasons. Using a SSR linkage map, the multiple QTL model (MQM) method of software package MapQTL version 4.0 was employed to map and analyze QTLs. Based on the whole genome sequence, the genomic regions harboring QTLs related to plant height were analyzed using comparative mapping and BLAST soft. 【Result】 QTL mapping analysis was conducted for three cucumber traits related to plant height, length of the main stem internode, and node number of main stem, in this study. Eleven QTLs were detected for these traits. These QTLs were mapped on chromosomes 1, 2, 5 and 6, respectively. Their LOD values varied between 3.03 and 12.73, which explained 6.2%-32.1% of the phenotypic variation. Five QTLs explained phenotypic variation more than 10%. Three QTLs (27.3% ) were found to be expressed consistently under four cropping seasons in greenhouse cultivation. QTL cluster was detected on Chr.1. 【Conclusion】 Based on the results in this study, it is speculated that there are at least four genes controlling plant height of cucumber, de gene controlling the determinate habit in cucumber was found in the region of QTLs mapped on the long-arm of Chr.6.

Key words: cucumber , plant height , quantitative trait locus (QTL) , recombinant inbred lines (RILs) , sequence analysis

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