Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (22): 4543-4551.doi: 10.3864/j.issn.0578-1752.2012.22.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Relationship Between the Expression of Genes Encoding Resistance-related Enzymes and the Resistance to Sclerotinia sclerotiorum in Resynthesized Brassica napus with High Level of Resistance

 WAN  Hua-Fang, LIU  , YAO  , MEI  Jia-Qin, DING  Yi-Juan, LIANG  , YING  , QU  Cun-Min, LU  , KUN  , LI  Jia-Na, QIAN  , WEI    

  1. College of Agronomy and Biotechnology, Southwest University/ Engineering Research Center of South Upland Agriculture, Ministry of Education/Chongqing Rapeseed Engineering and Technology Research Center, Chongqing 400716
  • Received:2012-09-04 Online:2012-11-15 Published:2012-10-08

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Abstract: 【Objective】 The relationship between the expression of genes encoding resistance-related enzymes and the resistance to S. sclerotiorum in resynthesized B. napus RB165 with high level of resistance was investigated.【Method】 RB165 was employed to investigate sclerotinia resistance using detached leaf and stem assay and to monitor the expression of genes encoding resistance-related enzymes (oxalate oxidase, Cu/Zn superoxidase dismutase, β-1,3-glucanase and chitinase) after sclerotinia inoculation with qRT-PCR, together with the resistant parental adopter (RP), four natural rapeseed lines with diverse levels of resistance.【Result】The leaf resistance was highly and positively correlated to that of the stem (r = 0.93) among 6 accessions. The resistance of RB165 was lower than that of RP, but significantly higher than that of natural rapeseed. The pattern of gene relative expression detected with qRT-PCR at 0, 6, 12, 24, 36 hours post inoculation (hpi) of S. sclerotiorum significantly differed in leaf, but the dynamics was similar among 6 accessions, i.e. OXO was inhibited, Cu/Zn SOD was inhibited at the beginning of inoculation, and then activated, PR2 was strongly induced twice and PR3 was dramatically activated. 【Conclusion】 RB165 was more resistant to S. sclerotiorum than natural rapeseed. No significant relationship was detected between S. sclerotiorum resistance and the expression of OXO, Cu/Zn SOD, PR2 and PR3.

Key words: Brassica incana, resynthesized Brassica napus, Sclerotinia sclerotiorum, pathogenesis related genes, qRT-PCR

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