Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (21): 4465-4474.doi: 10.3864/j.issn.0578-1752.2012.21.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Cloning, Expression and Bioinformatics Analysis of Porcine CatSperB and CatSperG Genes

 SONG  Cheng-Yi, ZHOU  Jia-Qing, FENG  Xiao-Jun, XIE  Yu-Xiu, LI  Qing-Ping, WU  Han, GAO  Bo, WANG  Xiao-Yan   

  1. 1.扬州大学动物科学与技术学院，江苏扬州225009
  • Received:2012-07-11 Online:2012-11-01 Published:2012-07-27

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Abstract: 【Objective】The aim of the current study is to confirm the existence of porcine CatSperB and CatSperG genes, and investigate the protein structures, evolutionary relationship and the spatial-temporal expression profiles of CatSperB and CatSperG. 【Method】The in silico and molecular cloning was used to identify the full length cDNAs of porcine CatSperB and CatSperG, and the spatial-temporal expression profile was investigated by qualitative and fluorescence quantitative RT-PCR. 【Result】The in silico transcripts of 3 508 bp CatSperB and 3 715 bp CatSperG were identified, and they contain 3 330 bp and 3 483 bp ORFs of CatSperB and CatSperG, respectively, and the sequences were confirmed by TA cloning. The sequence similarity of coding sequences (CDS) of porcine CatSperB and CatSperG with human, cattle, horse, and dog, and other animals was above 80%. CatSperB is a 125.79 kD and stable protein, while CatSperG is a 133.40 kD and unstable protein. Both CatSperB and CatSperG contain seven conservative trans-membrane domains, and a coiled-coil motif was also identified in the C terminal of CatSperG, but this motif was not found in CatSperB. The porcine CatSperB and CatSperG displayed higher degree of homology with the orthologs of cattle, dog and horse, and lower homology with those of human and mouse. The RT-PCR analysis showed that CatSperB and CatSperG were detected mainly in testis, but CatSperB also expressed in other tissues. The mRNA expression of CatSperB and CatSperG was significantly improved at the around stage of spermatogenesis (Day 60), puberty (Day 90) and sex maturity (Day 150) (P＜0.05). 【Conclusion】The cDNA clones of CatSperB and CatSperG, and a series of bioinformatics parameters of these proteins were obtained. Seven conservative trans-membrane domains in CatSperB and CatSperG and the homology among species were revealed. Furthermore, results of this study had proved that CatSperB and CatSperG expressed mainly in tests, and the change of mRNA expression was paralleled with sexual development of boar.

Key words: pig, CatSperB gene, CatSperG gene, cloning, spatial-temporal expression

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