Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (21): 4475-4483.doi: 10.3864/j.issn.0578-1752.2012.21.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Characterization of Tissue Expression and Cloning of Nuclear Factor I/B Gene in Chinese Merino (Xinjiang Junken Type)

 RONG  恩Guang, YU  Lei, YANG  Hua, ZHANG  Yong-Sheng, MA  Chun-Ping, LI  Hui, WANG  Ning   

  1. 1.东北农业大学动物科学技术学院，哈尔滨 150030
    2.新疆农垦科学院畜牧兽医研究所，新疆石河子 832000
    3.新疆西部牧业股份有限责任公司，新疆石河子 832000
  • Received:2012-03-05 Online:2012-11-01 Published:2012-05-18

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Abstract: 【Objective】The objective of this study was to explore the expression level of nuclear factor I/B gene in various sheep tissues, and clone the full-length coding sequence (CDS) of sheep NFIB gene in Chinese Merino (Xinjiang Junken type).【Method】Tissue expression profile of NFIB gene was detected by semi-quantitative reverse transcription PCR (SqRT-PCR). The full-length CDS region of NFIB gene was amplified by RT-PCR, cloned into T vector, and sequenced. The sequence was analyzed by using bioinformatics software.【Result】SqRT-PCR analysis showed that the NFIB gene was ubiquitously expressed in sheep internal organs and skin with different degrees. Comparatively, it was highly expressed in skin. The skin expression of NFIB gene in super fine wool strain had significant difference in different seasons (P＜0.05), but no significant difference in skins from various anatomic locations. There was no significant difference in the NFIB gene expression of body side skin among the 6 sheep strains/breeds (P＞0.05). Sequence analysis indicated that the sheep NFIB gene generated three NFIB protein isoforms of 420, 375 and 345 aa by alternative splicing.【Conclusion】NFIB gene is highly expressed in the skin of Chinese Merino (Xinjiang Junken type), and its expression in the skin of super fine wool strain has significant difference in different seasons. Sheep NFIB has at least three protein isoforms. This study not only has laid a foundation for clarifying the function of sheep NFIB gene, but also provided a theoretical basis for creating high quality merino sheep.

Key words: NFIB gene, gene expression, Chinese merino, gene cloning, protein isoform, super fine wool strain

[1]杨  华, 杨永林, 刘守仁, 王  宁, 李  辉, 石国庆, 张永胜, 冯  静. 中国美利奴羊(新疆军垦型)品系间羊毛理化性能及性状相关分析. 中国畜牧兽医, 2011, 38(6): 37-40.

Yang H, Yang Y L, Liu S R, Wang N, Li H, Shi G Q, Zhang Y S, Feng J. Correlation analysis of the physical and chemical properties of wool among strains of Chinese Merino (Xinjiang Junken type). China Animal Husbandry and Veterinary Medicine, 2011, 38(6): 37-40. (in Chinese)

[2]Chaudhry A Z, Lyons G E, Gronostajski R M. Expression patterns of the four nuclear factorⅠgenes during mouse embryogenesis indicate a potential role in development. Developmental Dynamics, 1997, 208(3): 313-325.

[3]Kruse U, Sippel A E. Transcription factor nuclear factor I proteins form stable homo- and heterodimers. Federation of European Biochemical Societies Letters, 1994, 348(1): 46-50.

[4]Goyal N, Knox J, Gronostajski R M. Analysis of multiple forms of nuclear factor I in human and murine cell lines. Molecular and Cellular Biology, 1990, 10(3): 1041-1048.

[5]Kruse U, Sippel A E. The genes for transcription factor nuclear factor I give rise to corresponding splice variants between vertebrate species. Journal of Molecular Biology, 1994, 238(5): 860-865.

[6]Osada S, Imagawa M, Nishihara T. Organization of gene structure and expression of nuclear factor 1 family in rat. DNA Sequence, 2005, 16(2): 151-155.

[7]Gronostajski R M. Roles of the NFI/CTF gene family in transcription and development. Gene, 2000, 249(1/2): 31-45.

[8]Steele-Perkins G, Plachez C, Butz K G, Yang G, Bachurski C J, Kinsman S L, Litwack E D, Richards L J, Gronostajski R M. The transcription factor gene Nfib is essential for both lung maturation and brain development. Molecular and Cellular Biology, 2005, 25(2): 685-698.

[9]Grunder A, Ebel T T, Mallo M, Schwarzkopf G, Shimizu T, Sippel A E, Schrewe H. Nuclear factor I-B (Nfib) deficient mice have severe lung hypoplasia. Mechanisms of Development, 2002, 112(1/2): 69-77.

[10]Moon H G, Hwang K T, Kim J A, Kim H S, Lee M J, Jung E M, Ko E, Han W, Noh D Y. NFIB is a potential target for estrogen receptor- negative breast cancers. Molecular Oncology, 2011, 5(6): 538-544.

[11]丁振华, 郭  玲, 郑  莉, 吕  超, 何英杰. Hsa-miR-365在UVB诱导HaCaT凋亡中的作用及靶基因NFIB的验证. 生物物理学报, 2009, 16(25): 147-148.

Ding Z H, Guo L, Zheng L, Lü C, He Y J. The role of Hsa-miR-365 in HaCaT apoptosis induced by UVB and the validation of target genes of NFIB. Acta Biophysica Sinica, 2009, 16(25): 147-148. (in Chinese)

[12]Perez-Casellas L A, Wang X, Howard K D, Rehage M W, Strong D D, Linkhart T A. Nuclear factorⅠtranscription factors regulate IGF binding protein 5 gene transcription in human osteoblasts. Biochimica et Biophysica Acta, 2009, 1789(2): 78-87.

[13]Dooley A L, Winslow M M, Chiang D Y, Banerji S, Stransky N, Dayton T L, Snyder E L, Senna S, Whittaker C A, Bronson R T, Crowley D, Barretina J, Garraway L, Meyerson M, Jacks T. Nuclear factor I/B is an oncogene in small cell lung cancer. Genes Development, 2011, 25(14): 1470-1475.

[14]de Jong R N, van der Vliet P C. Mechanism of DNA replication in eukaryotic cells: cellular host factors stimulating adenovirus DNA replication. Gene, 1999, 236(1): 1-12.

[15]Grunder A, Qian F, Ebel T T, Mincheva A, Lichter P, Kruse U, Sippel A E. Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor nuclear factor one. Gene, 2003, 304: 171-181.

[16]Hsu Y C, Osinski J, Campbell C E, Litwack E D, Wang D, Liu S, Bachurski C J, Gronostajski R M. Mesenchymal nuclear factor I B regulates cell proliferation and epithelial differentiation during lung maturation. Developmental Biology, 2011, 354(2): 242-252.

[17]Lin K K, Chudova D, Hatfield G W, Smyth P, Andersen B. Identification of hair cycle-associated genes from time-course gene expression profile data by using replicate variance. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(45): 15955-15960.

[18]Kim J, Johnson K, Chen H J, Carroll S, Laughon A. Drosophila Mad binds to DNA and directly mediates activation of vestigial by decapentaplegic. Nature, 1997, 388(6639): 304-308.

[19]Yingling J M, Das P, Savage C, Zhang M, Padgett R W, Wang X F. Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(17): 8940-8944.

[20]Paus R, Cotsarelis G. The biology of hair follicles. New England Journal of Medicine, 1999, 341(7): 491-497.

[21]Galbraith H. Fundamental hair follicle biology and fine fibre production in animals. Animal, 2010, 4(09): 1490-1509.

[22]Schlake T. Determination of hair structure and shape. Seminars in Cell and Developmental Biology, 2007, 18(2): 267-273.

[23]Ideker T, Thorsson V, Ranish J A, Christmas R,Buhler J, Eng J K, Bumgarner R, Goodlett D R, Aebersold R, Hood L. Integrated genomic and proteomic analyses of a systematically perturbed metabolic network. Science, 2001, 292(5518): 929-934.

[24]汪诗平, 王艳芬, 陈佐忠. 影响内蒙古细毛羊羊毛生产的主要因素. 草业学报, 2001, 10(2): 78-86.

Wang S P, Wang Y F, Chen Z Z. The factors influencing wool production of Inner Mongolia Fine sheep. Acta Prataculturae Sinica, 2001, 10(2): 78-86. (in Chinese)

[25]李  勇, 狄  江, 拉扎提, 李武臣, 陈为江, 苏爱国. 超细型羊毛季节性生长规律研究. 家畜生态学报, 2008, 29(5): 36-38.

Li Y, Di J, Lazhati, Li W C, Chen W J, Su A G. Study on seasonal growth of super fine wool. Acta Ecologae Animalis Domastici, 2008, 29(5): 36-38. (in Chinese)

[26]刘志红, 任立明, 郭  英, 胡晓湘, 崔文涛, 李  宁, 尹  俊, 李金泉. 毛囊的周期性变化和分子调控及其在绒山羊上的研究进展. 中国畜牧兽医, 2009, 36(4): 103-107.

Liu Z H, Ren L M, Guo Y, Hu X X, Cui W T, Li N, Yin J, Li J Q. The cyclical variation and regulation of hair follicles and the study on cashmere goat. China Animal Husbandry and Veterinary Medicine, 2009, 36(4): 103-107. (in Chinese)

[27]Gaussin A, Modlich U, Bauche C, Niederlander N J, Schambach A, Duros C, Artus A, Baum C, Cohen-Haguenauer O, Mermod N. CTF/NF1 transcription factors act as potent genetic insulators for integrating gene transfer vectors. Gene Therapy, 2012, 19(1): 15-24.
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