Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (20): 4216-4223.doi: 10.3864/j.issn.0578-1752.2012.20.011

• HORTICULTURE • Previous Articles     Next Articles

Effects of Exogenous Salicylic Acid on Nitrate Reduction and Assimilation in Chinese Chive Under Weak Light

 LI  Lin-Yan, WANG  Jun-Ling, WANG  Mei, GAO  Zhi-Kui   

  1. 1.河北农业大学园艺学院，河北保定 071000
    2.河北农业大学生命科学学院，河北保定 071000
  • Received:2012-04-06 Online:2012-10-15 Published:2012-08-31

PDF

934

Cited

1

Abstract: 【Objective】The effect of exogenous salicylic acid (SA) on accumulation of nitrate, the key enzyme activity of nitrogen metabolism, the apparent electron transport rate (ETR) and the main amino acids and soluble protein was studied in Chinese chive under the weak light in order to understand the effects of exogenous salicylic acid on nitrate reduction and assimilation.【Method】With Chinese chive as an experimental material, two treatment factors  including SA (3.0 mmol•L-1) and light intensity (the weak light and natural light) were designed in the experiment. 【Result】Under the weak light, exogenous SA pretreatment reduced the decrease in the key enzyme activity of nitrogen metabolism (nitrate reductase (NR), glufamine synthetase (GS), glutamic- oxaloacetic-transaminase (GOT) and glutamate-pyruvate-transaminase (GPT)), while decreased nitrate accumulation. Compared with no SA treatments，NR and GS activities in SA treatments increased significantly , 23.7% and 12.3%, respectively. Also, the content of chlorophyll (Chl), ETR , the content of free amino acids and soluble protein of weak light were enhanced after SA application. In addition, SA increased the content of most free amino acid components under weak light, with tryptophan and serine as the most effective, reaching 89.8% and 50.6%, respectively, and at the same time reduced the free amino acids and soluble protein ratio (A/P). 【Conclusion】Weak light reduced the capacity of the nitrogen assimilation and material production, however, exogenous SA spraying on leaves obviously improved nitrate reduction and assimilation, which, meanwhile, brought transaminations into play vigorously, and promoted turning NO3- into free amino acid and dissolvable protein. Improvement of nitrate reduction and assimilation might be a key reason for reducing nitrate accumulating into the vacuole of Chinese chive under weak light.

Key words: salicylic acid, shading, Chinese chive, nitrate reduction and assimilation, nitrogen metabolism

[1]高秀瑞，陈凤敏，刁春英，潘秀清，武彦荣，李　冰，陈贵林. 不同形态氮素替代硝态氮对蔬菜硝酸盐含量变化的影响. 华北农学报，2008, 23(6): 208-211.

Gao X R, Chen F M, Diao C Y, Pan X Q, Wu Y R, Li B, Chen G L. Effect of partial replacement of nitrate in solution by amino acid and urea on accumulation of nitrate in non-heading Chinese cabbage and lettuce. Acta Agriculturae Boreali-Sinica, 2008, 23(6): 208-211. ( in Chinese)

[2]宋艳霞，杨文钰，李卓玺，雍太明，刘 岚. 套作遮荫对大豆不同品种苗期氮代谢的影响. 中国油料作物学报, 2010, 32(3): 390-394.

Song Y X, Yang W Y, Li Z X, Yong T M, Liu L. Effect of maize-soybean relay cropping shade on nitrogen metabolism of soybean seedlings. Chinese Journal of Oil Crop Sciences, 2010, 32(3): 390-394. ( in Chinese)

[3]Hecht U, Oelmüller R, Schmidt S, Mohr H. Action of light, nitrate and ammonium on the levels of NADH and ferredoxin-dependent glutamate synthases in the cotyledons of mustard seedlings. Planta, 1988, 175: 130-138.

[4]于 莎，王友华，周治国，吕丰娟，刘敬然，马伊娜，陈 吉，阿布都克尤木•阿不都热孜克. 花铃期遮阴对棉花氮素代谢的影响及其机制. 作物学报, 2011, 37(10): 1879-1887.

Yu S, Wang Y H, Zhou Z G, Lü F J, Liu J R, Ma Y N, Chen J, Abudukeyoumu A. Effect of shade on nitrogen metabolism and its mechanism in cotton plant at flowering and boll-forming stage. Acta Agronomica Sinica, 2011, 37(10): 1879-1887. ( in Chinese)

[5]孟雪娇，邸  昆，丁国华. 水杨酸在植物体内的生理作用研究进展. 中国农学通报, 2010, 26(15): 207-214.

Meng X J, Di K, Ding G H. Progress of study on the physiological role of salicylic acid in plant. Chinese Agricultural Science Bulletin, 2010, 26(15): 207-214. ( in Chinese)

[6]Belinda A, Barbaros N. Effects of cold and salicylic acid treatments on nitrate reductase activity in spinach leaves, Turkish Journal of Biology, 2011, 35: 443-448.

[7]曹岩坡，高志奎，何俊萍，王  梅，高荣孚. 外源水杨酸对韭菜硝酸盐还原与同化的影响. 园艺学报, 2009, 36(3): 415-420.

Cao Y P, Gao Z K, He J P, Wang M, Gao R F. Effects of exogenous salicylic acid on nitrate accumulation and reduction and assimilation in the leaves of chinese chive. Acta Horticulturae Sinica, 2009, 36(3): 415-420. ( in Chinese)

[8]Singh B, Usha K. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation, 2003, 39: 137-141.

[9]Jain A, Srivastava H S. Effect of salicylic acid on nitrate reductase activity in maize seedlings. Plant Physiology, 1981, 51:339.

[10]刘  伟，艾希珍，梁文娟，王洪涛，刘升学，郑  楠．低温弱光下水杨酸对黄瓜幼苗光合作用及抗氧化酶活性的影响．应用生态学报, 2009, 20(2): 441-445．

Liu W, Ai X Z, Liang W J, Wang H T, Liu S X, Zheng N. Effects of salicylic acid on the leaf photosynthesis and antioxidant enzyme activities of cucumber seedlings under low temperature and light intensity. Chinese Journal of Applied Ecology, 2009, 20(2): 441-445．( in Chinese)

[11]霍 捷，高志奎，王俊玲，赵 飞. 高温胁迫下外源水杨酸对韭菜硝酸盐累积及PSⅡ光能吸收利用的影响. 中国农学通报，2011, 27(6): 133-137.

Huo J, Gao Z K, Wang J L, Zhao F. Effects of exogenous salicylic acid on nitrate accumulation and light absorption and utilization of photosystemⅡin the leaves of chinese chive under high temperature stress. Chinese Agricultural Science Bulletin, 2011, 27(6): 133-137. ( in Chinese)

[12]郝敬虹，易 旸，尚庆茂，董春娟，张志刚. 水杨酸处理对干旱胁迫下黄瓜幼苗氮素同化及其关键酶活性的影响. 园艺学报，2012, 39(1): 81-90.

Hao J H, Yi Y, Shang Q M, Dong C J, Zhang Z G. Effect of exogenous salicylic acid on nitrogen assimilation of cucumber seedling under drought stress. Acta Horticulturae Sinica, 2012, 39(1): 81-90. ( in Chinese)

[13]孙春杰，徐 杰，刘成连，原永兵. 水杨酸诱导黄瓜幼苗抗冷性的基因表达. 中国农学通报, 2009, 25(10): 178-182.

Sun C J, Xu J, Liu C L, Yuan Y B. Gene expression of chilling tolerance induced by salicylic acid in cucumber seedling. Chinese Agricultural Science Bulletin, 2009, 25(10): 178-182. (in Chinese)

[14]张以顺，黄 霞，陈云凤. 植物生理学试验教程. 北京: 高等教育出版社, 2009.

Zhang Y S, Huang X, Chen Y F. Plant Physiology Experiment Tutorial. Beijing: Higher Education Press, 2009. (in Chinese)

[15]吴良欢，蒋式洪，陶勤南. 植物转氨酶(GOT和GPT)活度比色测定方法及其应用. 土壤通报, 1998, 29(3): 136-138.

Wu L H, Jiang S H, Tao Q N. Plant aminotransferase (GOT and GPT) activity the colorimetric determination method and its application. Chinese Journal of Soil Science, 1998, 29(3): 136-138. (in Chinese)

[16]Mackown C T, Jakson W A, Volk R G. Partioning of previously accumulated nitrate to translocation, reduction, and efflux in corn roots. Planta, 1983, 157: 8-14.

[17]卢凤刚，陈贵林，吕桂云，任良玉. 不同供氮水平对韭菜产量和品质的影响. 园艺学报, 2005, 32(1): 131-133.

Lu F G, Chen G L, Lü G Y, Ren L Y. Effects of different nitrogen concentrations on yield and quality of Chinese chive. Acta Horticulturae Sinica, 2005, 32(1): 131-133. ( in Chinese)

[18]王 强，钟旭华，黄农荣，郑海波. 光、氮及其互作对作物碳氮代谢的影响研究进展. 广东农业科学, 2006(2): 37-40.

Wang Q, Zhong X H, Huang N R, Zheng H B. Interactions of nitrogen with light in the photosynthetic traits and metabolism of carbon and nitrogen of crop. Guangdong Agricultural Sciences, 2006 (2): 37-40. ( in Chinese)

[19]Bowsher C G, Long D M, Oaks A, Rothstein S J. Effect of light/dark cycles on expression of nitrate assimilatory genes in maize shoots and roots. Journal of Plant Physiology, 1991, 95: 281-285.

[20]泰兹(美), 奇格尔(美). 宋纯鹏,王学路等译. 植物生理学. 第四版. 北京: 科学出版社, 2009: 236-243.

Taiz L, Zeiger E. Translated by Song C P, Wang X L, et al. Plant Physiology. Fourth edition. Beijing: Science Press, 2009: 236-243. (in Chinese)

[21]沈国栋. 水杨酸调节怀槐培养细胞合成异黄酮与碳氮代谢的关系研究[D]. 合肥: 合肥工业大学, 2004.

Shen G D. Study on the relationship between isoflavone synthesis and carbon- and nitrogen-metabolism regulated by salicylic acid in cultured cells of Maackia amurensis[D]. Hefei:  Hefei University of Technology, 2004. (in Chinese)

[22]El-Tayeb M A. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, 2005, 45(3): 215-224.

[23]Ananiera E A, Alexieva V S, Popova L. Treatment with salicylic acid decreases the effects of paraquat on photosynthesis. Journal of Plant Physiology, 2002, 159: 685-693.

[24]王利军，李家承，刘允芬，刘琪瑾，黄卫东，石玉林. 高温干旱胁迫下水杨酸和钙对柑橘光合作用和叶绿素荧光的影响. 中国农学通报, 2003, 19(6): 185-189.

Wang L J, Li J C, Liu Y F, Liu Q J, Huang W D, Shi Y L. Effects of salicylic acid and CaCl2 on photosynthesis and chlorophyll fluorescence of citrus under heat and drought stress. Chinese Agricultural Science Bulletin, 2003, 19(6): 185-189. ( in Chinese)

[25]黄清泉，孙  歆，张年辉，冯  鸿，袁  澍，代其林，梁厚果，杜林方，林宏辉．水杨酸对水分胁迫黄瓜幼苗叶片生理过程的影响．西北植物学报, 2004, 24(12): 2202-2207．

Huang Q Q, Sun X, Zhang N H, Feng H, Yuan S, Dai Q L, Liang H G, Du L F, Lin H H. Effects of salicylic acid on leaves of cucumber seedlings under water stress. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(12): 2202-2207. (in Chinese)

[26]陈仕江，钟国跃，徐金辉. 黄连生育期间可溶性糖和氨基酸含量动态的研究. 中国中药杂志, 2005, 30(17): 1324-1327.

Chen S J, Zhong G Y, Xu J H. Dynamic change of soluble saccharides and amino acids during the growing period of Coptis chinensis. China Journal of Chinese Material Medicine, 2005, 30(17): 1324-1327. ( in Chinese)

[27]陈贵林，高秀瑞. 氨基酸和尿素替代硝态氮对水培不结球白菜和生菜硝酸盐含量的影响. 中国农业科学, 2002, 35(2): 187-191.

Chen G L, Gao X R. Effect of partial replacement of nitrate by amino acid and urea on nitrate content of nonheading Chinese cabbage and lettuce in hydroponics. Scientia Agricultura Sinica, 2002, 35(2): 187-191. (in Chinese)

[28]Miller A J, Fan X R, Shen Q R, Smith S J. Amino acids and nitrate as signals for the regulation of nitrogen acquisition. Journal of Experimental Botany, 2008, 59(1): 111-119.

[29]周诗毅，陈晓菁，黄建国，何光源. 八种氨基酸对水稻硝酸盐吸收的影响. 生物技术, 2009, 19(4): 58-60.

Zhou S Y, Chen X Q, Huang J G, He G Y. Effects of eight amino acids on the nitrate uptake in rice. Biological Technology, 2009, 19(4): 58-60. ( in Chinese)

[30]Migge A, Bork C, Hell R, Becker T W. Negative regulation of nitrate reductase gene expression by glutamine or as paragine accumulating in leaves of sulfur-deprived tobacco. Planta, 2000, 211: 587-595.

[31]彭新湘，李明启. 光呼吸代谢物乙醇酸、乙醛酸和草酸对烟草叶片硝酸还原的影响. 植物生理学报, 1987, 13(2): 182-189.

Peng X X, Li M Q. Effects of photorespiratory metabolites glycolate, glyoxylate and oxalate on nitrate reduction in tobacco leaf. Acta Phytophysiologica Sinica, 1987, 13(2): 182-189. (in Chinese)
[1] YU QiLong,HAN YingYan,HAO JingHong,QIN XiaoXiao,LIU ChaoJie,FAN ShuangXi. Effect of Exogenous Spermidine on Nitrogen Metabolism of Lettuce Under High-Temperature Stress [J]. Scientia Agricultura Sinica, 2022, 55(7): 1399-1410.
[2] WANG Juan,CHEN HaoNing,SHI DaChuan,YU TianYi,YAN CaiXia,SUN QuanXi,YUAN CuiLing,ZHAO XiaoBo,MOU YiFei,WANG Qi,LI ChunJuan,SHAN ShiHua. Functional Analysis of AhNRT2.7a in Response to Low-Nitrogen in Peanut [J]. Scientia Agricultura Sinica, 2022, 55(22): 4356-4372.
[3] HU YaLi,NIE JingZhi,WU Xia,PAN Jiao,CAO Shan,YUE Jiao,LUO DengJie,WANG CaiJin,LI ZengQiang,ZHANG Hui,WU QiJing,CHEN Peng. Effect of Salicylic Acid Priming on Salt Tolerance of Kenaf Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(14): 2696-2708.
[4] ZHAO Ke,ZHENG Lin,DU MeiXia,LONG JunHong,HE YongRui,CHEN ShanChun,ZOU XiuPing. Response Characteristics of Plant SAR and Its Signaling Gene CsSABP2 to Huanglongbing Infection in Citrus [J]. Scientia Agricultura Sinica, 2021, 54(8): 1638-1652.
[5] HAN ZhanYu,WU ChunYan,XU YanQiu,HUANG FuDeng,XIONG YiQin,GUAN XianYue,ZHOU LuJian,PAN Gang,CHENG FangMin. Effects of High-Temperature at Filling Stage on Grain Storage Protein Accumulation and Its Biosynthesis Metabolism for Rice Plants Under Different Nitrogen Application Levels [J]. Scientia Agricultura Sinica, 2021, 54(7): 1439-1454.
[6] WANG JinFeng,WANG ZhuangZhuang,GU FengXu,MOU HaiMeng,WANG Yu,DUAN JianZhao,FENG Wei,WANG YongHua,GUO TianCai. Effects of Nitrogen Fertilizer and Plant Density on Carbon Metabolism, Nitrogen Metabolism and Grain Yield of Two Winter Wheat Varieties [J]. Scientia Agricultura Sinica, 2021, 54(19): 4070-4083.
[7] ZHANG JingYun,LIU YuNuo,WANG ZhaoHao,PENG AiHong,CHEN ShanChun,HE YongRui. Analysis of Resistance Mechanism of CiNPR4 Transgenic Plants to Citrus Canker [J]. Scientia Agricultura Sinica, 2021, 54(18): 3871-3880.
[8] LI Ming,LI YingChun,NIU XiaoGuang,MA Fen,WEI Na,HAO XingYu,DONG LiBing,GUO LiPing. Effects of Elevated Atmospheric CO2 Concentration and Nitrogen Fertilizer on the Yield of Summer Maize and Carbon and Nitrogen Metabolism After Flowering [J]. Scientia Agricultura Sinica, 2021, 54(17): 3647-3665.
[9] QIN XiuJuan,QI JingJing,DOU WanFu,CHEN ShanChun,HE YongRui,LI Qiang. Identification of Rboh Family and the Response to Hormone and Citrus Bacterial Canker in Citrus [J]. Scientia Agricultura Sinica, 2020, 53(20): 4189-4203.
[10] KONG FanLin,LI Yuan,TANG MengQi,MA ManPeng,FU Tong,DIAO QiYu,CHENG SiYuan,TU Yan. Effects of Amino Acid Deficiency on Growth Development, Dietary Nutrients Digestion and Metabolism in Heifers [J]. Scientia Agricultura Sinica, 2020, 53(2): 418-430.
[11] CHEN QinCao,DAI WeiDong,LIN ZhiYuan,XIE DongChao,LÜ MeiLing,LIN Zhi. Effects of Shading on Main Quality Components in Tea (Camellia Sinensis (L) O. Kuntze) Leaves Based on Metabolomics Analysis [J]. Scientia Agricultura Sinica, 2019, 52(6): 1066-1077.
[12] HUANG XinHui,GAO Jia,REN BaiZhao,ZHAO Bin,LIU Peng,ZHANG JiWang. Effects of Phytase Q9 on Yield Formation of Summer Maize Shading in the Field [J]. Scientia Agricultura Sinica, 2019, 52(19): 3309-3322.
[13] ZHANG Xiang, WANG Jian, PENG Sheng, RUI QiuZhi, LI LiNan, CHEN Yuan, CHEN Yuan, CHEN DeHua. Relationship Between Temperature, Soil Moisture, and Insecticidal Protein Content in Bt Cotton Boll Shell and the Mechanism of Nitrogen Metabolism [J]. Scientia Agricultura Sinica, 2018, 51(7): 1261-1271.
[14] GONG XiangWei, HAN HaoKun, ZHANG DaZhong, LI Jing, WANG Meng, XUE ZhiHe, YANG Pu, GAO XiaoLi, FENG BaiLi. Effects of Nitrogen Fertilizer on Dry Matter Accumulation, Transportation and Nitrogen Metabolism in Functional Leaves of Broomcorn Millet at Late Growth Stage [J]. Scientia Agricultura Sinica, 2018, 51(6): 1045-1056.
[15] QianQian ZHOU,HuaRong QIU,XiaoWen HE,XianPu WANG,XiuXia LIU,BaoHua LI,ShuJing WU,XueSen CHEN. MdWRKY40 Mediated Improvement of the Immune Resistance of Apple and Arabidopsis thaliana to Botryosphaeria dothidea [J]. Scientia Agricultura Sinica, 2018, 51(21): 4052-4064.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd