Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 3955-3964.doi: 10.3864/j.issn.0578-1752.2012.19.006

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effects of Soybean Continuous Cropping on Microbial and Soil Enzymes in Soybean Rhizosphere

 GU  Yan, QIU  Qiang, WANG  Zhen-Min, CHEN  Xi-Feng, WU  Chun-Sheng   

  1. 1.吉林农业大学作物研究中心/大豆区域技术创新中心，长春 130118
    2.吉林省农业科学院，长春 130124
  • Received:2012-02-07 Online:2012-10-01 Published:2012-07-19

PDF

1220

Cited

17

Abstract: 【Objective】The objective of the experiment was to determine the effects of continuous and alternate cropping on microbe community structures and soil enzyme activities in soybean, and to provide a scientific basis for the obstacles of continuous cropping of soybean. 【Method】Rhizosphere soils of different growth stages was used to characterize the community structure of soil microbe based on phospholipid fatty acid (PLFA) method, and the contents of soil microbial biomass carbon and enzyme activities were determined. 【Result】In the soils of alternate and continuous cropping, the total PLFAs biomass, the microbial biomass carbon contents, the activities of urease and invertase all decreased, and the ratios of fungi and bacteria were enhanced, and the activities of catalase had no significant difference among the treatments. There were different influences on microbial and soil enzymes activities in different years of continuous cropping and growth stage, which closely related with the number and type of root exudates and straw decomposing matter. The correlation analysis showed that there was a positive relationship significantly between the total PLFAs biomass and microbial biomass carbon content and the activity of soil urease. There was no relationship between the total PLFAs biomass and the activity of soil catalase and invertase.【Conclusion】The microbial community structures and enzyme activities were changed by continuous and alternate soybean. The obstacles of continuous soybean were the result of the comprehensive effect of soil micro-ecological system and environmental factor.

Key words: soybean, phospholipid fatty acid, continuous cropping, community structure

[1]Susser J N, Uzzell J G. Control of the soybean cyst nematode by crop rotation in combination with a nematicide. Journal of Nematology, 1991,23(3):344-347.

[2]王金龙, 徐 冉, 陈存来, 王彩洁. 大豆连作下土壤环境条件变化的概述. 大豆科学,2000,19(4):367-371.

Wang J L, Xu R, Chen C L, Wang C J. General review in the study of barrier mechanism caused by continuous soybean cropping. Soybean Science, 2000,19(4):361-367.(in Chinese)

[3]Liu X B, Herbert S J. Fifteen years of research examining cultivation of continuous soybean in northeast China: A review. Field Crops Research, 2002,79(6):1-7.

[4]阮维斌, 王敬国, 张福锁. 连作障碍因素对大豆养分吸收和固氮作用的影响. 生态学报,2003,23(1):22-29.

Ruan W B, Wang J G, Zhang F S. The effect of continous cropping factors on soybean seedling growth and nitrogen fixation. Acta Ecologica Sinica,2003,23(1):22-29.(in Chinese)

[5]王树起, 韩晓增, 乔云发, 王守宇, 李晓慧, 许艳丽. 寒地黑土大豆轮作与连作不同年限土壤酶活性及相关肥力因子的变化. 大豆科学,2009, 28(4):611-615.

Wang S Q, Han X Z, Qiao Y F, Wang S Y, Li X H, Xu Y L. Variation of soil enzymes activity and relevant nutrients at different years of soybean (Glycine Max L.) rotation, alternate and continuous cropping. Soybean Science, 2009, 28(4):611-615.(in Chinese)

[6]季尚宁, 肖玉珍. 土壤灭菌对连作大豆生长发育的影响. 东北农业大学学报,1996,27(4):326-329.

Ji S N, Xiao Y Z. Effects of sterized soil from soybean and cornstubbie field on the growth development of soybean. Journal of Northeast Agricultural University, 1996,27(4):326-329.(in Chinese)

[7]台莲梅, 张红梅, 闫风云, 辛惠普. 重迎茬对大豆根际土壤微生物数量的影响. 土壤肥料,2003(6):41-43. 

Tai L M, Zhang H M, Yan F Y, Xin H P. Effect of the amount of soil microbe in soybean rhizospher under the condition of soybean continuous or alternate cropping. Soils and Fertilizers, 2003(6):41-43.(in Chinese)

[8]马春梅, 唐远征, 季尚宁. 作物定位轮作体系长期试验研究(Ⅱ)不同轮作方式对大豆田土壤微生物数量的影响. 东北农业大学学报,2004,35(6):645-650.

Ma C M, Tang Y Z, Ji S N. Long-term crop rotation research (Ⅱ)The impact on soil microorganisms quantity of the soybean field of different crop rotation ways. Journal of Northeast Agricultural University, 2004,35(6):645-650.(in Chinese)

[9]邹 莉, 袁晓颖, 李 玲, 王欣宇. 连作对大豆根部土壤微生物的影响研究. 微生物学杂志,2005,25(2):27-30.

Zou L, Yuan X Y, Li L, Wang X Y. Effects of continuous cropping on soil microbes on soybean root. Journal of Microbiology, 2005,25(2):27-30.(in Chinese)

[10]刘金波, 许艳丽, 李春杰, 魏 巍, 马丽丽. 长期连作对大豆根际真菌主要类群的影响. 农业系统科学与综合研究,2009,25(1):105-113.

Liu J B, Xu Y L, Li C J, Wei W, Ma L L. Effect of soybean long term continual cropping on main groups of rhizosphere fungi. System Sciences and Comprehensive Studies in Agriculture, 2009,25(1): 105-113.(in Chinese)

[11]陈中宽, 黄复民, 郭桂清, 张清华, 郑海春, 卢亚东, 崔文华. 大豆连作土壤肥力变化与有害生物发生的关系. 中国农学通报,2006,22(7):373-376.

Chen Z K, Huang F M, Guo G Q, Zhang Q H, Zheng H C, Lu Y D, Cui W H. Therelation between soil fertility caused by continuous soybean and the harmful livings. Chinese Agricultural Science Bulletin, 2006,22(7):373-376.(in Chinese)

[12]Garbeva P, van Veen J A, van Elsas J D. Microbial diversity in soil: selection of microbial populations by plant and soil type and implications for disease suppressive ness. Annual Review of Phytopathology,2004,42:243-270.

[13]李春格, 李晓鸣, 王敬国. 大豆连作对土体和根际微生物群落功能的影响. 生态学报,2006,26(4):1144-1150.

Li C G, Li X M, Wang J G. Effect of soybean continuous cropping on bulk and rhizosphere soil microbial community function. Acta Ecologica Sinica, 2006,26(4):1144-1150.(in Chinese)

[14]刘金波, 许艳丽. 我国连作大豆土壤微生物研究现状. 中国油料作物学报, 2008,30(1):132-136.

Liu J B, Xu Y L. Current research of soil microbial of successive soybean cropping in China. Chinese Journal of Oil Crop Sciences, 2008,30(1):132-136.(in Chinese)

[15]Nsabimana D, Haynes R J, Wallis F M. Size, activity and catabolic diversity of the soil microbial biomass as affected by land use. Applied Soil Ecology,2004, 26(2):81-92.

[16]Elfstrand S, Hedlund K, Mårtensson A. Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring. Applied Soil Ecology, 2007, 35(3): 610-621.

[17]卜洪震, 王丽宏, 尤金成, 肖小平, 杨光立, 胡跃高, 曾昭海. 长期施肥管理对红壤稻田土壤微生物量碳和微生物多样性的影响. 中国农业科学,2010,42(16):3340-3347.

Bu H Z, Wang L H, You J C, Xiao X P, Yang G L, Hu Y G, Zeng Z H. Impact of long-term fertilization on the microbial biomass carbon and soil microbial communities in paddy red soil. Scientia Agricultura Sinica, 2010,42(16):3340-3347.(in Chinese)

[18]Acosta-Martínez V,  Acosta-Mercado D,  Sotomayor-Ramírez D,  Cruz-Rodríguez L.  Microbial communities and enzymatic activities under different management in semiarid soils. Applied Soil Ecology,2008, 38(3): 249-260.

[19]Bais H P, Weir T L, Perry L G, Gilroy S, Vivanco J M. The role of root exudates in rhizosphere interactions with plants and other organisms. Annual Review of Plant Biology,2006, 57: 233-266.

[20]Böhme L, Böhme F. Soil microbiological and biochemical properties affected by plant growth and different long-term fertilization. European Journal of Soil Biology, 2006,42(1):1-12.

[21]Stark C H, Condron L M, O’Callaghan M, Stewart A, Di H J. Differences in soil enzyme activities, microbial community structure and short-term nitrogen mineralization resulting from farm management history and organic matter amendments. Soil Biology and Biochemistry,2008,40(6):1352-1363.

[22]冯慧敏, 何红波, 白 震, 武叶叶, 郭柏栋, 张 明, 张旭东. 乙草胺的微生物降解及其对土壤磷脂脂肪酸特性的影响. 应用生态学报,2008,19(7):1585-1590.

Feng H M, He H B, Bai Z, Wu Y Y, Guo B D, Zhang M, Zhang X D. Microbial degradation of acetochlor in mollisol and the effects of acetochlor on the characteristics of soil phospholipid fatty acids. Chinese Journal of Applied Ecology, 2008, 19(7): 1585-1590. (in Chinese)

[23]齐鸿雁, 薛 凯, 张洪勋. 磷脂脂肪酸谱图分析方法及其在微生物生态学领域的应用. 生态学报,2003,23(8):1577-1582.

Qi H Y, Xue K, Zhang H X. Phospholipid fatty acid analysis and its applications in microbial ecology. Acta Ecologica Sinica, 2003,23(8):1577-1582.(in Chinese)

[24]中国科学院南京土壤研究所微生物室. 土壤微生物研究法. 北京:科学出版社,1985:65-83.

Microbiology Laboratory in Institute of Soil Science, Chinese Academy of Sciences. Methods for Studying Soil Microbe. Beijing: China Scientic Press,1985:65-83.(in Chinese)

[25]Wang Y P, Shi J Y, Wang H, Qi L, Chen X C, Chen Y X. The influence of soil Heavy metals pollution on soil microbial biomass, enzyme activity, and community composition near a copper smelter. Ecotoxicology and Environmental Safety,2007, 67(1): 75-81.

[26]于 树, 汪景宽, 李双异. 应用PLFA方法分析长期不同施肥处理对玉米地土壤微生物群落结构的影响. 生态学报, 2008, 28(9): 4221-4227.

Yu S, Wang J K, Li S Y. Effect of long-term fertilization on soil microbial community structure in corn field with the method of PLFA. Acta Ecologica Sinica, 2008,28(9):4221-4227.(in Chinese)

[27]冯慧敏, 武叶叶, 何红波, 张旭东. 内标法测定土壤中乙草胺残留的气相色谱法研究. 环境科学与技术, 2007,30(12):32-34.

Feng H M, Wu Y Y, He H B, Zhang X D. Study on the quantification of acetochlor residue in soil by internal standard method using GC-ECD. Environmental Science and Technology, 2007, 30(12): 32-34. (in Chinese)

[28]关松荫. 土壤酶及研究方法. 北京:农业出版社,1986:274-332.

Guan S Y. Soil Enzyme and Research Method. Beijing: China Agricultural Press,1986:274-332.(in Chinese)

[29]刘建国, 张 伟, 李彦斌, 孙艳艳, 卞新民. 新疆绿洲棉花长期连作对土壤理化性状与土壤酶活性的影响. 中国农业科学,2009,42(2):725-733.

Liu J G, Zhang W, Li Y B, Sun Y Y, Bian X M. Effects of long-term continuous cropping system of cotton on soil physical-chemical properties and activities of soil enzyme in oasis in Xinjiang. Scientia Agricultura Sinica, 2009,42(2):725-733. (in Chinese)

[30]Ibekwe A M, Papiernik S K, Can J Y, Yates S R, Yang C H, Crowley D E. Impact of fumigants on soil microbial communities. Applied Environment Microbiology,2001,67(3): 3245-3257.

[31]张洪勋, 王晓谊, 齐鸿雁. 微生物生态学研究方法进展. 生态学报,2003,23(5): 988-995.

Zhang H X, Wang X Y, Qi H Y. Development in research methods of microbial ecology. Acta Ecologica Sinica, 2003,23(5): 988-995.(in Chinese)

[32]张淑香, 高子勤, 刘海玲. 连作障碍与根际微生态研究Ⅲ.土壤酚酸物质及其生物学效应. 应用生态学报,2000,11(5):741-744.

Zhang S X, Gao Z Q, Liu H L. Continuous cropping obstacle and rhizospheric microecology Ⅲ.Soil phenolic acids and their biological effect. Chinese Journal of Applied Ecology, 2000,11(5):741-744.(in Chinese)

[33]刘金波, 许艳丽, 吕国忠, 李春杰, 赵志慧, 魏 巍. 黑土区不同轮作系统大豆根际镰孢菌种群结构和数量. 大豆科学, 2009, 28(1): 97-102

Liu J B, Xu Y L, Lv G Z, Li C J, Zhao Z H, Wei W. Black soil region Fusarium population structure and quantity in soybean rhizosphere of different rotation system. Soybean Science, 2009,28(1):97-102.(in Chinese)

[34]Bååth E, Anderson T H. Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA based techniques. Soil Biology and Biochemistry, 2003,35:955-963.

[35]毕明丽, 宇万太, 姜子绍, 马 强, 张 璐, 徐永刚. 利用PLFA方法研究不同土地利用方式对潮棕壤微生物群落结构的影响. 中国农业科学,2010,43(9):1834-1842.

Bi M L, Yu W T, Jiang Z S, Ma Q, Zhang L, Xu Y G. Study of the effects of different land use patterns on microbial community structure in aquic brown soil by utilizing PLFA method. Scientia Agricultura Sinica, 2010,43(9):1834-1842.(in Chinese)

[36]Li C G, Li X M, Kong W D, Wu Y, Wang J G. Effect of monoculture soybean on soil microbial community in the Northeast China. Plant and Soil,2010,330:423-433.

[37]Guo Z Y, Kong C H, Wang J G, Wang Y F. Rhizosphere isoflavones (daidzein and genistein) levels and their relation to the microbial community structure of mono-cropped soybean soil in field and controlled conditions. Soil Biology and Biochemistry, 2011, 43: 2257-2264.

[38]殷勤燕, 陈宗泽, 杨振明, 戴秉利, 邹永久. 大豆重迎茬土壤微生物生物量碳的研究. 大豆科学,1996,15(4):357-361.

Yin Q Y, Chen Z Z, Yang Z M, Dai B L, Zou Y J. Study on biomass of soil microorgamisns of soybean follows soybean or follows next crops. Soybean Science, 1996,15(4):357-361.(in Chinese)

[39]刘素慧, 刘世琦, 张自坤, 尉 辉, 齐建建, 段吉锋. 大蒜连作对其根际土壤微生物和酶活性的影响. 中国农业科学, 2010, 43(5): 1000-1006.

Liu S H, Liu S Q, Zhang Z K, Wei H, Qi J J, Duan J F. Influence of garlic continuous cropping on rhizosphere soil microorganisms and enzyme activities. Scientia Agricultura Sinica, 2010,43(5):1000-1006. (in Chinese)

[40]傅慧兰, 邹永久, 韩丽梅, 闫 飞, 刘金萍. 大豆连作土壤障碍因素研究Ⅱ.连作土壤酶活性与肥力因素间的相关性分析. 大豆科学,1995,15(4):332-339.

Fu H L, Zou Y J, Han L M, Yan F, Liu J P. Effect of continuous soybean cropping on obstacle factors of soil. Soybean Science, 1995,15(4):332-339.(in Chinese)

[41]Lin R Y, Wang H B, Guo X K, Ye C Y, He H B, Zhou Y, Lin W X. Impact of applied phenolic acids on the microbes, enzymes and available nutrients in paddy soils. Allelopathy Journal, 2011,28(2): 225-236.

[42]Li P D, Dai C C, Wang X X, Zhang T L, Chen Y. Variation of soil enzyme activities and microbial community structure in peanut monocropping system in subtropical China. African Journal of Agricultural Research, 2012,7(12):1870-1879.
[1] DONG YongXin,WEI QiWei,HONG Hao,HUANG Ying,ZHAO YanXiao,FENG MingFeng,DOU DaoLong,XU Yi,TAO XiaoRong. Establishment of ALSV-Induced Gene Silencing in Chinese Soybean Cultivars [J]. Scientia Agricultura Sinica, 2022, 55(9): 1710-1722.
[2] LI YiLing,PENG XiHong,CHEN Ping,DU Qing,REN JunBo,YANG XueLi,LEI Lu,YONG TaiWen,YANG WenYu. Effects of Reducing Nitrogen Application on Leaf Stay-Green, Photosynthetic Characteristics and System Yield in Maize-Soybean Relay Strip Intercropping [J]. Scientia Agricultura Sinica, 2022, 55(9): 1749-1762.
[3] GUO ShiBo,ZHANG FangLiang,ZHANG ZhenTao,ZHOU LiTao,ZHAO Jin,YANG XiaoGuang. The Possible Effects of Global Warming on Cropping Systems in China XIV. Distribution of High-Stable-Yield Zones and Agro-Meteorological Disasters of Soybean in Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(9): 1763-1780.
[4] MA XiaoYan,YANG Yu,HUANG DongLin,WANG ZhaoHui,GAO YaJun,LI YongGang,LÜ Hui. Annual Nutrients Balance and Economic Return Analysis of Wheat with Fertilizers Reduction and Different Rotations [J]. Scientia Agricultura Sinica, 2022, 55(8): 1589-1603.
[5] JIANG FenFen, SUN Lei, LIU FangDong, WANG WuBin, XING GuangNan, ZHANG JiaoPing, ZHANG FengKai, LI Ning, LI Yan, HE JianBo, GAI JunYi. Geographic Differentiation and Evolution of Photo-Thermal Comprehensive Responses of Growth-Periods in Global Soybeans [J]. Scientia Agricultura Sinica, 2022, 55(3): 451-466.
[6] YAN Qiang,XUE Dong,HU YaQun,ZHOU YanYan,WEI YaWen,YUAN XingXing,CHEN Xin. Identification of the Root-Specific Soybean GmPR1-9 Promoter and Application in Phytophthora Root-Rot Resistance [J]. Scientia Agricultura Sinica, 2022, 55(20): 3885-3896.
[7] WANG QiaoJuan,HE Hong,LI Liang,ZHANG Chao,CAI HuanJie. Research on Soybean Irrigation Schedule Based on AquaCrop Model [J]. Scientia Agricultura Sinica, 2022, 55(17): 3365-3379.
[8] YUAN Cheng,ZHANG MingCong,WANG MengXue,HUANG BingLin,XIN MingQiang,YIN XiaoGang,HU GuoHua,ZHANG YuXian. Effects of Intertillage Time and Depth on Photosynthetic Characteristics and Yield Formation of Soybean [J]. Scientia Agricultura Sinica, 2022, 55(15): 2911-2926.
[9] ZHAO DingLing,WANG MengXuan,SUN TianJie,SU WeiHua,ZHAO ZhiHua,XIAO FuMing,ZHAO QingSong,YAN Long,ZHANG Jie,WANG DongMei. Cloning of the Soybean Single Zinc Finger Protein Gene GmSZFP and Its Functional Analysis in SMV-Host Interactions [J]. Scientia Agricultura Sinica, 2022, 55(14): 2685-2695.
[10] GONG XiaoYa,SHI JiBo,FANG Ling,FANG YaPeng,WU FengZhi. Effects of Flooding on Soil Chemical Properties and Microbial Community Composition on Farmland of Continuous Cropped Pepper [J]. Scientia Agricultura Sinica, 2022, 55(12): 2472-2484.
[11] REN JunBo,YANG XueLi,CHEN Ping,DU Qing,PENG XiHong,ZHENG BenChuan,YONG TaiWen,YANG WenYu. Effects of Interspecific Distances on Soil Physicochemical Properties and Root Spatial Distribution of Maize-Soybean Relay Strip Intercropping System [J]. Scientia Agricultura Sinica, 2022, 55(10): 1903-1916.
[12] HanXi LIU,Hao LÜ,GuangYu GUO,DongXu LIU,Yan SHI,ZhiJun SUN,ZeXin ZHANG,YanJiao ZHANG,YingNan WEN,JieQi WANG,ChunYan LIU,QingShan CHEN,DaWei XIN,JinHui WANG. Effect of rhcN Gene Mutation on Nodulation Ability of Soybean Rhizobium HH103 [J]. Scientia Agricultura Sinica, 2021, 54(6): 1104-1111.
[13] JiaJia LI,HuiLong HONG,MingYue WAN,Li CHU,JingHui ZHAO,MingHua WANG,ZhiPeng XU,Yin ZHANG,ZhiPing HUANG,WenMing ZHANG,XiaoBo WANG,LiJuan QIU. Construction and Application of Detection Model for the Chemical Composition Content of Soybean Stem Based on Near Infrared Spectroscopy [J]. Scientia Agricultura Sinica, 2021, 54(5): 887-900.
[14] Qian CAI,ZhanXiang SUN,JiaMing ZHENG,WenBin WANG,Wei BAI,LiangShan FENG,Ning YANG,WuYan XIANG,Zhe ZHANG,Chen FENG. Dry Matter Accumulation, Allocation, Yield and Productivity of Maize- Soybean Intercropping Systems in the Semi-Arid Region of Western Liaoning Province [J]. Scientia Agricultura Sinica, 2021, 54(5): 909-920.
[15] SHAO MeiQi,ZHAO WeiSong,SU ZhenHe,DONG LiHong,GUO QingGang,MA Ping. Effect of Bacillus subtilis NCD-2 on the Growth of Tomato and the Microbial Community Structure of Rhizosphere Soil Under Salt Stress [J]. Scientia Agricultura Sinica, 2021, 54(21): 4573-4584.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd